scholarly journals Transfection of Capsaspora owczarzaki  using calcium-phosphate precipitation v1 (protocols.io.p4adqse)

protocols.io ◽  
2018 ◽  
Author(s):  
Helena Parra ◽  
N ria ◽  
Alberto Perez ◽  
Aleksandra Kozyczkowska ◽  
Sebasti n
Keyword(s):  
Calcium Phosphate ◽  
Calcium Phosphate Precipitation

Duration of Dentinal Tubule Occlusion Formed by Calcium Phosphate Precipitation Method: In vitro Evaluation Using Synthetic Saliva

1995 ◽  
Vol 74 (10) ◽  
pp. 1709-1714 ◽  
Author(s):  
T. Suge ◽  
K. Ishikawa ◽  
A. Kawasaki ◽  
M. Yoshiyama ◽  
K. Asaoka ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Precipitation Method ◽  
Dentinal Tubule ◽  
In Vitro Evaluation ◽  
Calcium Phosphate Precipitation Method ◽  
Tubule Occlusion ◽  
Calcium Phosphate Precipitation

Molecular Basis of Salivary Proline-rich Protein and Peptide Synthesis: Cell-free Translations and Processing of Human and Macaque Statherin mRNAs and Partial Amino Acid Sequence of their Signal Peptides

1987 ◽  
Vol 66 (2) ◽  
pp. 462-466 ◽  
Author(s):  
F.G. Oppenheim ◽  
D.I. Hay ◽  
D.J. Smith ◽  
G.D. Offner ◽  
R.F. Troxler
Keyword(s):  
Calcium Phosphate ◽  
Macaca Fascicularis ◽  
Electrophoretic Analysis ◽  
Proteolytic Processing ◽  
Oral Environment ◽  
Reticulocyte Lysate ◽  
Polyprotein Precursor ◽  
Phosphate Salts ◽  
Calcium Phosphate Precipitation

Acidic proline-rich phosphoproteins and phosphopeptides are abundant components of parotid and submandibular salivary secretions in man and in the subhuman primate, Macaca fascicularis. The major acidic proline-rich proteins and the proline-rich phosphopeptide, statherin, of man and macaques have been shown to be potent inhibitors of calcium phosphate precipitation and are thought to function in the oral environment by maintaining saliva supersaturated with respect to calcium phosphate salts. Little is known about the biosynthesis of these proline-rich phosphoproteins and peptides, and the aim of the present work was to determine the structural relationship between statherin precursors and native human and macaque statherin. RNA was isolated from human submandibular gland, and poly(A+) mRNA was selected by affinity chromatography on oligo(dT) cellulose and translated in a reticulocyte lysate. Electrophoretic analysis of the translation products revealed that this mRNA directed the synthesis of a large number of polypeptides with M,s ranging from 5000 to 70,000. Immunoprecipitates, prepared with an antiserum directed against human statherin, contained a single component with a Mr of 7800, approximately 2000 daltons larger than native statherin. Radiosequencing of the in vitro precursor of statherin in immunoprecipitates demonstrated the presence of a 19-residue signal peptide. These results suggest that statherin is derived from a unique structural gene, and does not result from proteolytic processing of a large polyprotein precursor.

Effect of Electrical Polarization on the Behavior of Bioactive Glass Containing MgO and B2O3 in SBF

2006 ◽  
Vol 309-311 ◽  
pp. 333-336
Author(s):  
Emiko Amaoka ◽  
Erik Vedel ◽  
Satoshi Nakamura ◽  
Yusuke Moriyoshi ◽  
Jukka I. Salonen ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Simulated Body Fluid ◽  
Bioactive Glass ◽  
Body Fluid ◽  
Charged Surface ◽  
Depolarization Current ◽  
Electrical Polarization ◽  
Thermally Stimulated Depolarization Current ◽  
Thermally Stimulated Depolarization ◽  
Calcium Phosphate Precipitation

We investigated the electrical polarizability of MgO and B2O3 containing bioactive glass (MBG). The MBG material with good manufacturing properties but low bioactivity was electrically polarized at a high dc field. The electrical polarizability of MBG was evaluated by thermally stimulated depolarization current (TSDC) measurements and immersion in simulated body fluid (SBF). The early precipitation of calcium phosphate on the negatively charged surface of the treated MBG demonstrated the increased bioactivity of the material and confirmed its polarizability. It is suggested that the electrical interactions between the polarized MBG and ions in SBF promoted the formation of the calcium phosphate precipitation. Accordingly, the increased bioactivity of the MBG in SBF is suggested to demonstrate the conversion of MBG into electrovector ceramics by the polarization treatment.

scholarly journals Real-Time Raman Monitoring of Calcium Phosphate Precipitation in a Semi-Batch Stirred Crystallizer

2018 ◽  
Vol 18 (3) ◽  
pp. 1622-1628 ◽  
Author(s):  
Bing Han ◽  
Marjatta Louhi-Kultanen
Keyword(s):  
Calcium Phosphate ◽  
Real Time ◽  
Calcium Phosphate Precipitation

Control of Calcium Phosphate Precipitation in Hydrogel

2007 ◽  
pp. 79-82
Author(s):  
Masanobu Kamitakahara ◽  
Shinichi Ogata ◽  
Masao Tanihara ◽  
Chikara Ohtsuki
Keyword(s):  
Calcium Phosphate ◽  
Calcium Phosphate Precipitation

Effect of temperature and reactant concentration on calcium phosphate precipitation

2020 ◽  
Vol 552 ◽  
pp. 125909
Author(s):  
Luisa R. Brasil ◽  
Marcela C. Rodrigues ◽  
Thiago L.R. Hewer ◽  
Bruna M. Fronza ◽  
Victor E. Arana-Chavez ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Effect Of Temperature ◽  
Reactant Concentration ◽  
Calcium Phosphate Precipitation

Phosphate Precipitation in Biofilms and Flocs

1983 ◽  
Vol 15 (3-4) ◽  
pp. 65-85 ◽  
Author(s):  
Erik Arvin ◽  
Gert Holm Kristensen
Keyword(s):  
Mathematical Model ◽  
Calcium Phosphate ◽  
Carbon Source ◽  
Practical Interest ◽  
Calcium Bicarbonate ◽  
Fixed Film ◽  
Phosphate Phase ◽  
Film Reactor ◽  
Calcium Phosphate Precipitation ◽  
Apatitic Phase

The experiments have confirmed that denitrification in biofilms may lead to calcium phosphate precipitation inside the biofilms due to the increased pH created by the denitrification reaction. This has been shown in a laboratory fixed film reactor with well defined geometry and feed with well defined substrates. The phosphate precipitate was in one case found similar to a carbonate containing apatitic phase. It accumulated in the bio-film to 9.3% P based on dry solids. A mathematical model has been developed relating the phosphate to nitrate molar removal rates to the concentration of phosphate, calcium, bicarbonate, the solubility of the solid phosphate phase and the type of carbon source used for denitrification. The model was supported by the experiments. The experiments show that the biofilm precipitation may be of significant practical interest, but factors as filamentous growth on the biofilm surface or precipitation of an easy soluble amorphous calcium phosphate can also make the biofilm precipitation insignificant. The precipitation effect is expected to be feasible in floes, if a homogeneous biomatrix with a thickness of more than approx. 100 µm exist in the floc substructure.

STUDY OF NICKEL ION RELEASE IN SIMULATED BODY FLUID FROM C+-IMPLANTED NICKEL TITANIUM ALLOY

2016 ◽  
Vol 23 (05) ◽  
pp. 1650045 ◽  
Author(s):  
MUHAMMAD AHSAN SHAFIQUE ◽  
G. MURTAZA ◽  
SHAHZAD SAADAT ◽  
ZEESHAN ZAHEER ◽  
MUHAMMAD SHAHNAWAZ ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Simulated Body Fluid ◽  
Body Fluid ◽  
Niti Alloy ◽  
Niti Shape Memory Alloy ◽  
Ion Release ◽  
Nickel Ion ◽  
Oxidation Layer ◽  
X Ray ◽  
Calcium Phosphate Precipitation

Nickel ion release from NiTi shape memory alloy is an issue for biomedical applications. This study was planned to study the effect of C[Formula: see text] implantation on nickel ion release and affinity of calcium phosphate precipitation on NiTi alloy. Four annealed samples are chosen for the present study; three samples with oxidation layer and the fourth without oxidation layer. X-ray diffraction (XRD) spectra reveal amorphization with ion implantation. Proton-induced X-ray emission (PIXE) result shows insignificant increase in Ni release in simulated body fluid (SBF) and calcium phosphate precipitation up to [Formula: see text][Formula: see text]ions/cm2. Then Nickel contents show a sharp increase for greater ion doses. Corrosion potential decreases by increasing the dose but all the samples passivate after the same interval of time and at the same level of [Formula: see text] in ringer lactate solution. Hardness of samples initially increases at greater rate (up to [Formula: see text][Formula: see text]ions/cm[Formula: see text] and then increases with lesser rate. It is found that [Formula: see text][Formula: see text]ions/cm2 ([Formula: see text] is a safer limit of implantation on NiTi alloy, this limit gives us lesser ion release, better hardness and reasonable hydroxyapatite incubation affinity.

Proteoglycan inhibition of calcium phosphate precipitation in liposomal suspensions

Glycobiology ◽  
1992 ◽  
Vol 2 (6) ◽  
pp. 571-578 ◽  
Author(s):  
Edward D. Eanes ◽  
Arthur W. Hailer ◽  
Ronald J. Midura ◽  
Vincent C. Hascall
Keyword(s):  
Calcium Phosphate ◽  
Calcium Phosphate Precipitation

Exceptional Fossil Conservation through Phosphatization

2014 ◽  
Vol 20 ◽  
pp. 59-82 ◽  
Author(s):  
James D. Schiffbauer ◽  
Adam F. Wallace ◽  
Jesse Broce ◽  
Shuhai Xiao
Keyword(s):  
Calcium Phosphate ◽  
Soft Tissues ◽  
Geochemical Processes ◽  
Comprehensive Description ◽  
Animal Evolution ◽  
Pore Fluid Chemistry ◽  
Type Preservation ◽  
Fossil Preservation ◽  
Calcium Phosphate Precipitation ◽  
Taphonomic Processes

This paper addresses the taphonomic processes responsible for fossil preservation in calcium phosphate, or phosphatization. Aside from silicification and rarer examples of carbonaceous compression, phosphatization is the only taphonomic mode claimed to preserve putative subcellular structures. Because this fossilization window can record such valuable information, a comprehensive understanding of its patterns of occurrence and the geochemical processes involved in the replication of soft tissues are critical endeavors. Fossil phosphatization was most abundant during the latest Neoproterozoic through the early Paleozoic, coinciding with the decline of non-pelletal phosphorite deposits. Its temporal abundance during this timeframe makes it a particularly valuable window for the study of early animal evolution. Several occurrences of phosphatization from the Ediacaran through the Permian Period, including Doushantuo-type preservation of embryo-like fossils and acritarchs, phosphatized gut tracts within Burgess Shale-type carbonaceous compressions, Orsten-type preservation of meiofaunas, and other cases from the later Paleozoic are reviewed. In addition, a comprehensive description of the geochemical controls of calcium phosphate precipitation from seawater is provided, with a focus on the rates of phosphate nucleation and growth, favorable nucleation substrates, and properties of substrate tissue and pore-fluid chemistry. It is hoped that the paleontological and geochemical summaries provided here offer a practical and valuable guide to the Neoproterozoic–Paleozoic phosphatization window.

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