Evaluating fixed-film nitrification systems in autotrophic mode: Enhanced biomass retention does not improve bioreactor performance during alkalinity induced stress

Use of fixed-film systems has shown promise towards improving the process stability of biological nitrogen removal (BNR). It allows for biofilm formation, which can offer enhanced resilience to environmental stressors...

Convergent Microbial Community Formation in Replicate Anaerobic Reactors Inoculated from Different Sources and Treating Ersatz Crew Waste

Future manned space travel will require efficient recycling of nutrients from organic waste back into food production. Microbial systems are a low-energy, efficient means of nutrient recycling, but their use in a life support system requires predictability and reproducibility in community formation and reactor performance. To assess the reproducibility of microbial community formation in fixed-film reactors, we inoculated replicate anaerobic reactors from two methanogenic inocula: a lab-scale fixed-film, plug-flow anaerobic reactor and an acidic transitional fen. Reactors were operated under identical conditions, and we assessed reactor performance and used 16s rDNA amplicon sequencing to determine microbial community formation. Reactor microbial communities were dominated by similar groups, but differences in community membership persisted in reactors inoculated from different sources. Reactor performance overlapped, suggesting a convergence of both reactor communities and organic matter mineralization. The results of this study suggest an optimized microbial community could be preserved and used to start new, or restart failed, anaerobic reactors in a life support system with predictable reactor performance.

Efficiency studies of modified IFAS-OSA system upgraded by an anoxic sludge holding tank

An upgraded integrated fixed-film activated sludge-oxic settling anoxic (IFAS-OSA) system is a new technology for reducing nutrients and excess sludge. The results showed that the average TN removal efficiency of the IFAS-OSA system was gradually increased up to 7.5%, while the PO4–3-P removal efficiency increased up-to 27%, compared with that of the IFAS system. The COD removal efficiency of the IFAS-OSA system was slightly increased up-to 5.4% and TSS removal efficiency increased up to 10.5% compared with the control system. Biomass yield coefficient (Yobs) in the IFAS and IFAS-OSA systems were 0.44 and 0.24 (gr MLSS/ gr COD). Hence, sludge production decreased by 45%. The average SVI was decreased by 48% in IFAS-OSA system compared with IFAS. This study demonstrated the better performance of the IFAS-OSA system compared to that of the IFAS system.

Sequencing Batch Integrated Fixed-Film Activated Sludge Membrane Process for Treatment of Tapioca Processing Wastewater

Tapioca processing industries are very popular in the rural community to produce a variety of foods as the end products. Due to their small scales and scattered locations, they require robust modular systems to operate at low capacity with minimum supervision. This study explores the application of a novel sequencing batch-integrated fixed-film activated sludge membrane (SB-IFASM) process to treat tapioca processing wastewater for reuse purposes. The SB-IFASM employed a gravity-driven system and utilizes biofilm to enhance biodegradation without requiring membrane cleaning. The SB-IFASM utilizes the biofilm as a secondary biodegradation stage to enhance the permeate quality applicable for reuse. A lab-scale SB-IFASM was developed, preliminarily assessed, and used to treat synthetic tapioca processing industry wastewater. The results of short-term filtration tests showed the significant impact of hydrostatic pressure on membrane compaction and instant cake layer formation. Increasing the pressure from 2.2 to 10 kPa lowered the permeability of clean water and activated sludge from 720 to 425 and from 110 to 50 L/m2·h bar, respectively. The unsteady-state operation of the SB-IFASM showed the prominent role of the bio-cake in removing the organics reaching the permeate quality suitable for reuse. High COD removals of 63–98% demonstrated the prominence contribution of the biofilm in enhancing biological performance and ultimate COD removals of >93% make it very attractive for application in small-scale tapioca processing industries. However, the biological ecosystem was unstable, as shown by foaming that deteriorated permeability and was detrimental to the organic removal. Further developments are still required, particularly to address the biological stability and low permeability.

Organic Matter Removal in a Simultaneous Nitrification-Denitrification Process Using Fixed-Film System.

This study focused on evaluating different support media for COD and nitrogen compound removal from an Upflow Anaerobic Sludge Blanket (UASB) reactor fed with swine wastewater. Maximum specific nitrification (MSNA) and denitrification (MSDA) activity tests were performed in two fixed-film systems with (1) polyurethane foam (R1) and (2) polyethylene rings (R2). The results showed that the R2 system performed more efficiently than R1, reaching organic matter removal of 77 ± 8% and nitrogen of 98 ± 4%, attributed to higher specific denitrifying activity recorded (5.3 ± 0.34 g NO3--N/g VTS ∙h). In this sense, MSDA tests indicated that the suspended biomass was responsible for at least 70% of nitrogen removal in the form of ammonium compared with 20% attributed to biomass in the form of biofilm. On the other hand, 40 ± 5% of initial nitrogen could not be quantified in the system effluents, but 10 ± 1% was attributed to loss by volatilization. According to the analyses, the previous information infers the development of simultaneous nitrification-denitrification (SND) routes. Respect to the analyses of microbial diversity and abundance in the biofilm of R2 rings, the presence of the genus Pseudomonas dominated the prokaryotic community of the system in 54.4%.

Pilot-scale study based on integrated fixed-film activated sludge process for cement industrial wastewater treatment

Due to the lack of freshwater resources in Egypt, cement wastewater treatment was performed to widen the range of the water used in irrigation to face the massive future water scarcity. In this study, integrated fixed-film activated sludge (IFAS) was used as a biological treatment method. A laboratory pilot was established as a simulation of the IFAS process. The scale-pilot consists of a primary sedimentation tank, an IFAS tank equipped with an air blower, and a final settling tank. Three experimental attempts were performed using 3 different bio-carriers. In the first trial, Luffa sponges were used as natural bio-carriers and polyurethane sponges (PU) as artificial bio-carriers in the second trial, in addition to a combination between Luffa and PU sponges as a hybrid bio-carrier in the third trial. After analyzing the physicochemical properties of wastewater at the national research center in Cairo, the removal efficiency of TSS (total suspended solids), COD (chemical oxygen demand) , BOD(biological oxygen demand), TN (total nitrogen), and TP (total phosphorous) was 94.5%, 87.8%, 90.8%, 75.9%, and 69.4%, respectively in case of using the combination between Luffa and PU sponges. It can be concluded that using IFAS process was effective for cement wastewater treatment and the effluent wastewater met the Egyptian code limitations for reuse in agriculture purposes.