Carbon-Ion Beam Radiosensitivity Study and Biological Responses of High-Yielding Rice Line, MR219-PL-5

The carbon ion-beam has emerged as a novel physical mutagen for creating genetic variability and crop improvement. In this study, seeds of a high-yielding pyramided rice line MR219-PL-5 were exposed to carbon ion beam irradiation at 10, 20, 40, 60, 80, and 100 Gy. The radiosensitivity test was conducted to determine the optimum dose of carbon ion beam irradiation based on the lethal dose 50% (LD50) using Sandwich Blotter Technique. The biological responses of carbon-ion beam irradiation were also observed in other characteristics such as germination rate (GeR), survival rate (SR), growth rate (GRoR), shoot length (SL), root length (RL), seedling height (SH), days to flowering (DTF), fertility rate (FR) and thousand-grains weight (TGW). Based on the polynomial curve of SR graph, the lethal dose 50% (LD50) value was 86.12 Gy. However, the optimum dose range of carbon ion-beam irradiation was between 40 and 60 Gy as these two doses recorded the highest SR, 63 and 67%, respectively. Furthermore, the shoulder dose in this study was 60 Gy since SR decreased significantly at higher doses. M1 individuals irradiated at 40 and 60 Gy had the best biological responses where significant differences were found for SR, SL, RL, GRoR, SH, DTF and FR at these two doses compared to the other doses. Further studies on M2 and M3 populations could help to identify potential individuals as well as to understand the inheritance of each trait of interest from one generation to the next.

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Evaluation of DNA Damage Response After Carbon-Ion Beam Irradiation in p53-Mutant Cancer Cells

International Journal of Radiation Oncology*Biology*Physics ◽

10.1016/j.ijrobp.2014.05.2274 ◽

2014 ◽

Vol 90 (1) ◽

pp. S786

Author(s):

N. Amornwichet ◽

T. Oike ◽

M. Komachi ◽

M. Isono ◽

H. Ogiwara ◽

...

Keyword(s):

Dna Damage ◽

Cancer Cells ◽

Dna Damage Response ◽

Ion Beam ◽

Beam Irradiation ◽

Ion Beam Irradiation ◽

Carbon Ion ◽

Damage Response ◽

Carbon Ion Beam

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Identification of Substitutions and Small Insertion-Deletions Induced by Carbon-Ion Beam Irradiation in Arabidopsis thaliana

Frontiers in Plant Science ◽

10.3389/fpls.2017.01851 ◽

2017 ◽

Vol 8 ◽

Cited By ~ 21

Author(s):

Yan Du ◽

Shanwei Luo ◽

Xin Li ◽

Jiangyan Yang ◽

Tao Cui ◽

...

Keyword(s):

Arabidopsis Thaliana ◽

Ion Beam ◽

Beam Irradiation ◽

Ion Beam Irradiation ◽

Carbon Ion ◽

Carbon Ion Beam

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The type of mutations induced by carbon-ion-beam irradiation of the filamentous fungus Neurospora crassa

Fungal Biology ◽

10.1016/j.funbio.2013.01.002 ◽

2013 ◽

Vol 117 (4) ◽

pp. 227-238 ◽

Cited By ~ 8

Author(s):

Liqiu Ma ◽

Yusuke Kazama ◽

Hirokazu Inoue ◽

Tomoko Abe ◽

Shin Hatakeyama ◽

...

Keyword(s):

Neurospora Crassa ◽

Filamentous Fungus ◽

Ion Beam ◽

Beam Irradiation ◽

Ion Beam Irradiation ◽

Carbon Ion ◽

Carbon Ion Beam

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Research of photosynthesis and genomewide resequencing on a yellow-leaf Lotus japonicus mutant induced by carbon ion beam irradiation

Grassland Science ◽

10.1111/grs.12216 ◽

2018 ◽

Vol 65 (1) ◽

pp. 41-48 ◽

Cited By ~ 2

Author(s):

Tao Cui ◽

Shanwei Luo ◽

Yan Du ◽

Lixia Yu ◽

Jiangyan Yang ◽

...

Keyword(s):

Ion Beam ◽

Lotus Japonicus ◽

Yellow Leaf ◽

Beam Irradiation ◽

Ion Beam Irradiation ◽

Carbon Ion ◽

Carbon Ion Beam

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Effects of Carbon Ion Beam Irradiation on Butanol Tolerance and Production of Clostridium acetobutylicum

Frontiers in Microbiology ◽

10.3389/fmicb.2020.602774 ◽

2020 ◽

Vol 11 ◽

Author(s):

Yue Gao ◽

Miaomiao Zhang ◽

Xiang Zhou ◽

Xiaopeng Guo ◽

Cairong Lei ◽

...

Keyword(s):

Cell Membrane ◽

Wild Type Strain ◽

Ion Beam ◽

Membrane Integrity ◽

Beam Irradiation ◽

Ion Beam Irradiation ◽

Butanol Tolerance ◽

Carbon Ion ◽

Cell Membrane Integrity ◽

Carbon Ion Beam

Clostridium acetobutylicum (C. acetobutylicum) has considerable potential for use in bioenergy development. Owing to the repeated use of traditional mutagenesis methods, the strains have developed a certain tolerance. The rheology of the bioprocess and the downstream processing of the product heavily depend on the ability of C. acetobutylicum mutants to produce butanol. Carbon ion beam irradiation has advantages over traditional mutation methods for fermentative production because of its dose conformity and superb biological effectiveness. However, its effects on the specific productivity of the strains have not been clearly understood. In this study, we screened five mutants through carbon ion beam irradiation; mutant Y217 achieved a butanol-production level of 13.67 g/L, exceeding that of wild-type strain ATCC 824 (i.e., 9.77 g/L). In addition, we found that the mutant maintained normal cell membrane integrity under the stimulation of 15 g/L butanol, whereas the intracellular macromolecules of wild-type strain ATCC 824 leaked significantly. Subsequently, we used the response surface methodology (RSM) to determine if the mutant cell membrane integrity improved the butanol tolerance. We verified that with the addition of butanol, the mutant could be fermented to produce 8.35 g/L butanol, and the final butanol concentration in the fermentation broth could reach 16.15 g/L. In this study, we proved that under butanol stress, mutant Y217 features excellent butanol production and tolerance and cell membrane integrity and permeability; no prior studies have attempted to do so. This will serve as an interesting and important illustration of the complexity of genetic control of the irradiation mutation of C. acetobutylicum strains. It may also prove to be useful in the bioengineering of strains of the mutant for use in the predevelopment stage.

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