In vitro propagation of plume cultivars selected in Amur Region, Russia

2021 ◽  
Vol 26 ◽  
Author(s):  
E.V. Nekrasov ◽  
◽  
L.A. Shelikhan ◽  
Keyword(s):  
Tissue Culture ◽  
Agar Medium ◽  
Shoot Proliferation ◽  
Amur Region ◽  
Proliferation Rate ◽  
Culture Initiation ◽  
Clonal Micropropagation ◽  
Lateral Buds ◽  
Culture Production

Results are presented on clonal micropropagation for three cultivars selected in Amur Region: 'Blagoveshchenskii chernosliv', 'Lyudmila', and 'Oranzhevaya rannyaya'. Tips of growing young shoots are preferable explants for the tissue culture production as compared to lateral buds of the same shoots. The Quoirin-Lepoivre (QL) agar medium supplemented with sorbitol (20 g/L) and 6-benzylaminopurine (BA, 3 mg/L) was used for the establishment of explants and tissue culture initiation. Shoot proliferation was conducted on a QL agar medium with a modified microelement and vitamin composition and supplemented with sucrose (30 g/L), BA (0.2, 0.5, or 2.0 mg/L) and indole-3-butyric acid (IBA, 0.04 mg/L). Optimal shoot proliferation was noted to be achieved by alternate cultivation cycles on the medium with various BA concentration: 2 mg/L for an increased proliferation rate and 0.2–0.5 mg/L for an increased microshoot length. Higher values of shoot proliferation (the proliferation rate and microshoot length) were found for cv. 'Blagoveshchenskij chernosliv' (1.6 and 1.9 respectively) as compared to cv. 'Lyudmila' (1.3 and 1.5) and cv. 'Oranzhevaya rannyaya' (1.4 and 1.2). In vitro rooting was achieved after a preliminary incubation of microshoots in the aqueous solution of IBA (15 mg/L) with the subsequent cultivation on the growth regulator-­free QL agar medium. The cultivar 'Blagoveshchenskij chernosliv' had higher values of rooting (5.8 roots per shoot and 11.6 cm of total root length) as compared to cv. 'Lyudmila' (4.6 and 6.6 respectively) and cv. 'Oranzhevaya rannyaya' (5.3 and 7.4). The protocol was used for production of own­rooted plants of the plum cultivars.

scholarly journals IN VITRO PROPAGATION OF THE OSTRICH FERN (Matteuccia struthiopteris)

1985 ◽  
Vol 65 (4) ◽  
pp. 1025-1032 ◽  
Author(s):  
BRIAN W. DYKEMAN ◽  
BRUCE G. CUMMING
Keyword(s):  
Tissue Culture ◽  
Cross Section ◽  
In Vitro Propagation ◽  
Shoot Proliferation ◽  
Inorganic Salts ◽  
Adenine Sulphate ◽  
Lateral Buds ◽  
Morphogenesis In Vitro ◽  
Half Strength

Methods were developed for the successful in vitro propagation of ostrich fern (Matteuccia struthiopteris (L.) Todaro) clones utilizing shoot tips derived by forcing lateral buds on the rhizome. Maximum shoot proliferation was attained with 6-furfurylaminopurine (kinetin) at 1.0 mg/L with half-strength Murashige and Skoog (MS) inorganic salts and sucrose, agar, NaH2PO4, adenine sulphate, i-inositol and thiamine∙HCl at 30 000, 4000, 85, 40, 100, 0.4 mg/L, respectively. Excellent frond and root development was achieved with half-strength MS salts and sucrose, agar, i-inositol and thiamine∙HCl at 7500, 4000, 100 and 0.4 mg/L, respectively. The methods developed were satisfactory for a cross section of clones. Morphogenesis in vitro was dependent on medium osmotic potential.Key words: Matteuccia struthiopteris, in vitro propagation, tissue culture, morphogenesis, fern (ostrich)

scholarly journals In vitro shoot proliferation of sweet cherry cultivars Karešova and Rivan

2008 ◽  
Vol 35 (No. 3) ◽  
pp. 95-98 ◽  
Author(s):  
J. Sedlák ◽  
F. Paprštein
Keyword(s):  
Tissue Culture ◽  
Sweet Cherry ◽  
Shoot Proliferation ◽  
Proliferation Rate ◽  
Future Research ◽  
Ms Medium ◽  
Culture Methods ◽  
Shoot Production ◽  
Sweet Cherry Cultivars

The objective of this study was to investigate the possibility of optimizing routine tissue culture methods to proliferate two sweet cherry cultivars Karešova and Rivan. Shoot tips of two genotypes were successfully established <i>in vitro</I>. Six proliferation MS media containing 1, 2 and 4 mg/l BAP (6-benzylaminopurine), 0.5 and 1 mg/l TDZ (thidiazuron) or 10 mg/l 2iP (6-(&gamma;, &gamma;-dimethylallylamino)purine) were tested. The highest proliferation rate (3.0 ± 0.1) was obtained for Rivan on MS medium containing 2 mg/l BAP. In the case of cultivar Karešova, any of the cytokinins tested did not promote satisfactory proliferation. The highest proliferation rate (1.6) achieved on MS medium with 2 mg/l 2iP is not sufficient for a larger scale <i>in vitro</i> shoot production. It was proved that different genotypes of sweet cherry do not respond in the same way during proliferation <i>in vitro</i>. Future research and testing of other media and plant growth regulators will be carried out.

scholarly journals Micropropagation ofOriganum acutidens(HAND.-MAZZ.) IETSWAART Using Stem Node Explants

2013 ◽  
Vol 2013 ◽  
pp. 1-3 ◽  
Author(s):  
Mehmet Ugur Yildirim
Keyword(s):  
Tissue Culture ◽  
Culture Media ◽  
Landscape Management ◽  
Shoot Proliferation ◽  
Ornamental Plant ◽  
Peat Moss ◽  
Ms Medium ◽  
Regeneration Rate ◽  
Stem Node

Origanum acutidens(HAND.-MAZZ.) IETSWAART is a promising ornamental plant that can be widely used in landscape management. It is endemic to Eastern Anatolian region of Turkey. Tissue culture has not been used to micropropagate it. The study reports stem node explants from one-week-old seedlings of the plant for successful micropropagation. The stem nodes were cultured on MS medium containing 0.6, 1.2, 1.8, and 2.4 mg/L BAP with 0.2 mg/L NAA. Visible effects of culture media on shoot proliferation were recorded. Shoot regeneration rate was maximum on MS medium containing 1.80 mg/L BAP-0.2 mg/L NAA. The micropropagated shoots were rooted on MS medium containing 0.2 mg/L NAA. All microrooted plantlets survived during acclimatisation on peat moss. It was concluded thatO. acutidenscan be successfully micropropagated underin vitroconditions.

scholarly journals Propagation Techniques for a Spineless Acacia wrightii

HortScience ◽  
2005 ◽  
Vol 40 (6) ◽  
pp. 1832-1837 ◽  
Author(s):  
Donita L. Bryan ◽  
Michael A. Arnold ◽  
R. Daniel Lineberger ◽  
W. Todd Watson
Keyword(s):  
Tissue Culture ◽  
Acetic Acid ◽  
Butyric Acid ◽  
Shoot Proliferation ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Root Number ◽  
Indole Butyric Acid ◽  
Tissue Culture Propagation

Three spineless phenotypes of Acacia wrightii G. Bentham ex A. Gray were identified with aesthetic landscape potential. Experiments in seed, cutting, grafting, and tissue culture propagation were undertaken to perpetuate this desired spineless phenotype. Germination percentages for mechanically scarified seeds ranged from 33% to 94%, however yield of spineless seedlings was low (0% to 34%). Sulfuric acid scarification for 10, 20, 30, or 60 minutes hastened and unified germination compared to nontreated seeds by 7 to 8 days. Vegetative propagation was successful for softwood cuttings. Rooting measures increased with auxin (2:1 indole butyric acid to naphthalene acetic acid) concentrations from 0 to 15000 mg·L–1, with maximum rooting percentage (70%), root number (9.2), and root length (12.4 cm) per softwood cutting at 15000 mg·L–1 auxin 8 weeks after treatment. Rooting was not successful for semi-hardwood or hardwood cuttings. Whip-and-tongue or T-bud grafting was not successful. Tissue culture of shoots from in vitro germinated seedlings indicated that shoot proliferation was greatest in Murashige and Skoog (MS) medium with 15 μm zeatin. The number of shoots that rooted in vitro increased with increasing concentrations of indole-3-butyric acid from 0 to 25 μm.

scholarly journals 027 MULTIPLICATION OF ROSE SPECIES IN VITRO

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 431d-431
Author(s):  
Yan Ma ◽  
David H. Byrne ◽  
Jing Chen ◽  
Amanda Byrne
Keyword(s):  
Basal Medium ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Multiple Shoots ◽  
Naphthalene Acetic Acid ◽  
Good Growth ◽  
Lateral Buds ◽  
Half Strength ◽  
The Media

Several rose species (Rosa rugosa, R. wichuraiana, R. setigera, R. laevigata, R. banksiae, R. roxburghii, R. odorata and hybrids) were employed to establish the appropriate nutrient media for shoot multiplication and root initiation of cultured shoots and to describe a procedure for the successful transfer to soil of plants obtained in vitro. Cultured shoot tips and lateral buds from different genotypes proliferated multiple shoots on a basal medium (MS salt, vitamins, glycine, sucrose and agar) supplemented with 0mg/l to 6mg/l 6-benzylamino purine (BA) and 0mg/l to 0.5 mg/l naphthalene acetic acid (NAA). Most rose species cultured in a modified MS medium supplemented with 2mg/l BA showed good growth and shoot proliferation. The buds nearest the apex exhibited the slowest rate of bud development. Root development was enhanced and shoot development inhibited by lowering the concentration of MS salts to quarter- and half-strength. With difficult-to-root species, rooting was improved by supplementing the media with auxin or giving them 3-7days of dark treatment.

scholarly journals CLONAL MICROPROPAGATION OF MALE-STERILE ZINNIA ELEGANS

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1124F-1124
Author(s):  
R.B. Rogers ◽  
M.A.L. Smith ◽  
R. Cowen
Keyword(s):  
Large Scale ◽  
Basal Medium ◽  
Shoot Proliferation ◽  
Zinnia Elegans ◽  
Scale Production ◽  
Male Sterile ◽  
Clonal Micropropagation ◽  
Large Scale Production ◽  
High Humidity Environment

The only method for large scale production of pure hybrid seed in Zinnia elegans involves the use of male sterile individuals. The male sterile trait, however, is a three gene recessive which at best produces only 50% male sterile progeny from seed. Since no method of clonal propagation is available, seed-produced female lines require labor intensive field roguing to insure removal of all normal flowered individuals. Clonal micropropagation was investigated as a means of mass producing male steriles for use as female lines. Sterilization procedures were developed for seed and axillary bud explants. Shoot proliferation media containing various levels of BAP, 2ip, and kinetin were screened using in vitro germinated seedling explants of the inbred line `Orange Starlight'. Microshoots demonstrated a high rooting percentage after 2 weeks on basal medium without growth regulators. Plantlets were easily acclimated in 1 to 2 weeks in a high humidity environment. In vitro derived plants of identified male sterile plants were phenotypically evaluated as to their suitability for use in field production.

IN VITRO PROPAGATION OF OTTAWA 3 APPLE ROOTSTOCK

1983 ◽  
Vol 63 (1) ◽  
pp. 183-188 ◽  
Author(s):  
E.-C. PUA ◽  
CALVIN CHONG ◽  
G. L. ROUSSELLE
Keyword(s):  
Culture Medium ◽  
Agar Medium ◽  
Shoot Proliferation ◽  
Normal Growth ◽  
Naphthalene Acetic Acid ◽  
Shoot Cultures ◽  
Apple Rootstock ◽  
In Vitro Proliferation ◽  
In Vitro Shoot Proliferation

Methods were developed for obtaining normal shoot cultures and for rapid in vitro proliferation and rooting of Ottawa 3 apple rootstock from meristem tips. The presence of naphthalene acetic acid (NAA) and benzyladenine (BA), both at concentrations of either 0.5 or 1.0 mg/L in the culture medium, was most effective for in vitro shoot proliferation, but growth was abnormal. Normal growth was achieved when shoots were cultured with a combination of 0.5 mg/L NAA, 0.5 mg/L BA and 5.0 mg/L gibberellic acid. One hundred percent rooting was achieved after 2 wk on agar medium supplemented with 6.25 mg/L indole butyric acid.Key words: Tissue culture, Malus, meristem cloning, growth regulators

scholarly journals Withania Coagulans (Stocks) Dunal: Biotechnological Achievements And Perspectives

2015 ◽  
Vol 23 (1) ◽  
pp. 5-12 ◽  
Author(s):  
Jaime A. Teixeira da Silva ◽  
Mafatlal M. Kher ◽  
Deepak Soner ◽  
M. Nataraj
Keyword(s):  
Tissue Culture ◽  
Medicinal Plant ◽  
Large Scale ◽  
Culture Initiation ◽  
Surface Sterilization ◽  
Withania Coagulans ◽  
Important Means ◽  
Important Medicinal Plant

AbstractWithania coagulans (Stocks) Dunal is an important medicinal plant of the Solanaceae. Biotechnological studies on this plant started in 2009 and are still in a nascent phase of development. Even so, some important advances have been made, particularly in the field of tissue culture, which is an important means for its large-scale propagation and in vitro conservation. This review focuses on methods for surface sterilization, culture initiation, multiplication, rooting and acclimatization of W. coagulans.

scholarly journals Establishment of an efficient in vitro propagation protocol for Sumac (Rhus coriaria L.) and confirmation of the genetic homogeneity

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Saleh Amiri ◽  
Reza Mohammadi
Keyword(s):  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
High Rate ◽  
Naphthalene Acetic Acid ◽  
Root Induction ◽  
Ms Medium ◽  
Lateral Buds ◽  
Rhus Coriaria ◽  
Four Levels

AbstractThe conventional reproduction methods are not efficient for regeneration of Sumac (Rhus coriaria L.). The purpose of this work was to study the micropropagation of R. coriaria using lateral buds as explant in Murashige and Skoog (MS) medium with different concentrations of plant growth regulator (PGRs). Four concentrations of Benzylaminopurine (BAP) in combination with three concentrations of indol-3-butyric acid (IBA) and 1.0 mg/L gibberellic acid (GA3) were tested for establishment and shoot multiplication. For root induction, IBA was used at four levels combined with 0, 0.5 and 1 mg/L of naphthalene acetic acid (NAA) in full and half strength of MS medium. BAP at 2 mg/L with 1 mg/L IBA was best, with 88.88% of establishment. The highest shoot proliferation (12.30 ± 0.30) was obtained in medium fortified with 2 mg/L BAP plus 0.5 mg/L IBA and the highest shoot length (8.50 cm) was obtained at 3 mg/L BAP plus 1 mg/L IBA. The highest rooting (100%) was observed in 1/2-strength MS medium containing 1 mg/L IBA with 0.5 mg/L NAA. In conclusion, an efficient protocol with high rate of proliferation and rooting is described for R. coriaria, which can be used in massive propagation.

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