scholarly journals Visualization of Borna Disease Virus Protein Interactions with Host Proteins using in situ Proximity Ligation Assay

2016 ◽  
Vol 3 (1) ◽  
pp. 11-23
Author(s):  
Jonas Johansson Wensman ◽  
Keyword(s):  
Disease Virus ◽  
Protein Interactions ◽  
Proximity Ligation Assay ◽  
Virus Protein ◽  
Host Proteins ◽  
Borna Disease Virus ◽  
Proximity Ligation ◽  
Borna Disease

scholarly journals Borna Disease in a Dog with Lethal Meningoencephalitis

1998 ◽  
Vol 36 (7) ◽  
pp. 2127-2130 ◽  
Author(s):  
Herbert Weissenböck ◽  
Norbert Nowotny ◽  
Patrick Caplazi ◽  
Jolanta Kolodziejek ◽  
Felix Ehrensperger
Keyword(s):  
In Situ Hybridization ◽  
Nucleotide Sequence ◽  
Disease Virus ◽  
Nested Pcr ◽  
Borna Disease Virus ◽  
Neurological Signs ◽  
Pcr Product ◽  
Borna Disease ◽  
Brain Tissues

A dog was euthanatized because of progressive neurological signs. Histologically, a nonsuppurative meningoencephalitis was found. By immunohistochemistry, in situ hybridization, and nested PCR procedures, Borna disease virus (BDV) antigen and BDV-specific RNA were demonstrated in brain tissues of the dog. The nucleotide sequence of the PCR product showed 94 to 98% homology to published BDV sequences. This is the first description of Borna disease in a dog.

scholarly journals Investigation of fatal human Borna disease virus 1 encephalitis outside the previously known area for human cases, Brandenburg, Germany – a case report

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Dennis Tappe ◽  
Kirsten Pörtner ◽  
Christina Frank ◽  
Hendrik Wilking ◽  
Arnt Ebinger ◽  
...  
Keyword(s):  
Disease Virus ◽  
Next Of Kin ◽  
Borna Disease Virus ◽  
Case Presentation ◽  
Recent Outbreak ◽  
Polymerase Chain ◽  
Clinical Awareness ◽  
Generation Sequencing ◽  
Borna Disease

Abstract Background The true burden and geographical distribution of human Borna disease virus 1 (BoDV-1) encephalitis is unknown. All detected cases so far have been recorded in Bavaria, southern Germany. Case presentation A retrospective laboratory and epidemiological investigation of a 2017 case of fatal encephalitis in a farmer in Brandenburg, northeast Germany, demonstrated BoDV-1 as causative agent by polymerase chain reaction, immunohistochemistry and in situ hybridization. Next-generation sequencing showed that the virus belonged to a cluster not known to be endemic in Brandenburg. The investigation was triggered by a recent outbreak of animal Borna disease in the region. Multiple possible exposures were identified. The next-of-kin were seronegative. Conclusions The investigation highlights clinical awareness for human BoDV-1 encephalitis which should be extended to all areas endemic for animal Borna disease. All previously diagnosed human cases had occurred > 350 km further south. Further testing of shrews and livestock with Borna disease may show whether this BoDV-1 cluster is additionally endemic in the northwest of Brandenburg.

scholarly journals Proximity Ligation Assay for Detecting Protein‐Protein Interactions and Protein Modifications in Cells and Tissues in Situ

2020 ◽  
Vol 89 (1) ◽  
Author(s):  
Marihan Hegazy ◽  
Eran Cohen‐Barak ◽  
Jennifer L. Koetsier ◽  
Nicole A. Najor ◽  
Constadina Arvanitis ◽  
...  
Keyword(s):  
Protein Interactions ◽  
Proximity Ligation Assay ◽  
Protein Protein Interactions ◽  
Protein Modifications ◽  
Proximity Ligation ◽  
In Cells

In Situ Proximity Ligation Assay (In Situ PLA) to Assess PTP-Protein Interactions

2016 ◽  
pp. 217-242 ◽  
Author(s):  
Sina Koch ◽  
Irene Helbing ◽  
Sylvia-Annette Böhmer ◽  
Makoto Hayashi ◽  
Lena Claesson-Welsh ◽  
...  
Keyword(s):  
Protein Interactions ◽  
Proximity Ligation Assay ◽  
Proximity Ligation

Using an in Situ Proximity Ligation Assay to Systematically Profile Endogenous Protein–Protein Interactions in a Pathway Network

2014 ◽  
Vol 13 (12) ◽  
pp. 5339-5346 ◽  
Author(s):  
Tzu-Chi Chen ◽  
Kuan-Ting Lin ◽  
Chun-Houh Chen ◽  
Sheng-An Lee ◽  
Pei-Ying Lee ◽  
...  
Keyword(s):  
Protein Interactions ◽  
Proximity Ligation Assay ◽  
Protein Protein Interactions ◽  
Pathway Network ◽  
Proximity Ligation ◽  
Endogenous Protein
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