scholarly journals THE EFFECT OF MULTIAXIAL DEFORMATION ON THE DYNAMICS OF BIODEGRADATION AND CELL COLONIZATION OF ALLOY WE43

2021 ◽  
Vol 20 (2) ◽  
pp. 76-84
Author(s):  
N. S. Martynenko ◽  
N. Yu. Anisimov ◽  
K. M. Novruzov ◽  
S. V. Dobatkin ◽  
M. V. Kiselevskiy ◽  
...  
Keyword(s):  
Magnesium Alloys ◽  
Stromal Cells ◽  
Degradation Rate ◽  
Materials Science ◽  
Multipotent Stromal Cells ◽  
Gas Formation ◽  
Digital Microscope ◽  
Cell Colonization ◽  
Alloy We43 ◽  
Mesenchymal Multipotent Stromal Cells

Introduction. The development of materials for bioresorbable implants is an urgent issue in medicine and materials science. Magnesium alloys are promising materials for this purpose. In particular, alloy WE43 (Mg-Y-Nd-Zr) has proven itself well in this field. However, the use of magnesium alloys is limited by a high degradation rate, which is often accompanied with nonuniform corrosion, which negatively affects the load bearing capacity of the product. In addition, the increased degradation rate usually seriously worsens the biocompatibility of magnesium alloys. Therefore, the study of the corrosion resistance of magnesium alloys, as well astheir biocompatibility, is an urgent task.Purpose of the study was to investigate the effect of multiaxial deformation (MAD), aimed at increasing the mechanical characteristics of the alloy WE43, on its biodegradation kinetics, as well as on cell colonization.Materials and methods. The alloy WE43 in two states  – homogenized (WE43 hom) and strengthened by MAD (WE43 MAD) was investigated in this work. The kinetics of biodegradation was investigated on an xCELLigence RTCA Systems analyzer. A method for estimating the volume of hydrogen was used to study the process of gas formation, which was recorded using an automated digital microscope LionheartTM FX. The corrosive medium was a solution based on Dulbecco’s Modified Eagle’s Medium. A culture of mesenchymal multipotent stromal cells was used to study the colonization of the alloy surface by cells.Results. MAD of the alloy WE43 leads to a decrease in the biodegradation rate and the intensity of gas formation. The period of stabilization of biodegradation for the alloy after the MAD is 16 hours versus 3 hours for the alloy after homogenization. In this case, the volume of released hydrogen was 65.0 ± 4.4  mm3H2/mm3 alloy and 211.0 ± ± 21.1 mm3H2/mm3 alloy for the alloy after MAD and homogenization, respectively. MAD improves the biocompatibility of the alloy WE43, stimulating the colonization of mesenchymal multipotent stromal cells.Conclusion. MAD reduces biodegradation and improves the biocompatibility of the alloy WE43, which makes it a promising medical material, including for the purposes of oncoorthopedics

Differentiation of Mesenchymal Multipotent Stromal Cells of the Lungs in Pneumofibrosis

2013 ◽  
Vol 154 (4) ◽  
pp. 537-543 ◽  
Author(s):  
E. G. Skurikhin ◽  
E. S. Khmelevskaya ◽  
O. V. Pershina ◽  
N. N. Ermakova ◽  
V. A. Krupin ◽  
...  
Keyword(s):  
Stromal Cells ◽  
Multipotent Stromal Cells ◽  
Mesenchymal Multipotent Stromal Cells

Cytoplasm and organelle transfer between mesenchymal multipotent stromal cells and renal tubular cells in co-culture

2010 ◽  
Vol 316 (15) ◽  
pp. 2447-2455 ◽  
Author(s):  
Egor Y. Plotnikov ◽  
Tatyana G. Khryapenkova ◽  
Svetlana I. Galkina ◽  
Gennady T. Sukhikh ◽  
Dmitry B. Zorov
Keyword(s):  
Stromal Cells ◽  
Multipotent Stromal Cells ◽  
Tubular Cells ◽  
Renal Tubular Cells ◽  
Organelle Transfer ◽  
Renal Tubular ◽  
Mesenchymal Multipotent Stromal Cells

scholarly journals ROLE OF MESENCHYMAL MULTIPOTENT STROMAL CELLS IN REMODELING OF BONE DEFECTS

2018 ◽  
Vol 20 (4) ◽  
pp. 515-522
Author(s):  
M. V. Kiselevsky ◽  
N. Yu. Anisimova ◽  
Yu. I. Dolzhikova ◽  
R. Ya. Vlasenko ◽  
F. S. Senatov ◽  
...  
Keyword(s):  
Stromal Cells ◽  
Bone Defects ◽  
Multipotent Stromal Cells ◽  
Mesenchymal Multipotent Stromal Cells

scholarly journals Morphology of the Myocardium after Experimental Bone Tissue Trauma and the Use of Extracellular Vesicles Derived from Mesenchymal Multipotent Stromal Cells

2021 ◽  
Vol 11 (11) ◽  
pp. 1206
Author(s):  
Igor Maiborodin ◽  
Marina Klinnikova ◽  
Sergey Kuzkin ◽  
Vitalina Maiborodina ◽  
Sergey Krasil’nikov ◽  
...  
Keyword(s):  
Blood Flow ◽  
Blood Vessels ◽  
Bone Tissue ◽  
Extracellular Vesicles ◽  
Stromal Cells ◽  
The Body ◽  
Simultaneous Increase ◽  
Multipotent Stromal Cells ◽  
Vascular Walls ◽  
Mesenchymal Multipotent Stromal Cells

The effect of extracellular vesicles (EVs) of various origins on the heart structures in the time of health and disease has been well studied. At the same time, data on the distribution of EVs throughout the body after introduction into the tissues and the possibility of the influence of these EVs on organs distant from the injection site are practically absent. It is also necessary to note a certain inconsistency in the results of various researchers: from articles on the direct absorption of EVs derived from mesenchymal multipotent stromal cells (MSC EVs) by cardiomyocytes to the data that the heart is inherently immune to drug delivery mediated by nanoparticles. In this regard, the morphological changes in the myocardium of outbred rabbits of both sexes weighing 3–4 kg were studied at various times after experimental trauma of the bone tissue in the proximal condyle of the tibia (PCT) and the use of MSC EVs. As a result of modeling the PCT defect, rabbits develop myocardial edema in the heart muscle by the 3rd day, their lymphatic vessels expand, and then, on the 7th day, the blood vessels become dilated. In the myocardium, the relative and absolute contents of neutrophils, erythrocytes, and macrophages increase, but the percentage of lymphocytes decreases. By day 10, almost all of these changes return to their initial values. The detected transformations of the myocardium are most likely due to the ingress of detritus with the blood flow from the PCT. The use of MSC EVs to influence the regeneration of damaged tissue of PCT promotes earlier dilatation of the blood vessels of the heart with pronounced diapedesis of erythrocytes or even hemorrhages, prolongation of edema, the formation of blood clots in vessels with obliteration of their lumen, sclerotic transformation of vascular walls and paravascular tissues. In the myocardium, the number density of neutrophils, the percentage of lymphocytes, and neutrophils become smaller, with a simultaneous increase in the relative numbers of erythrocytes and macrophages, and changes in the content of macrophages remained until the end of the observation—up to 10 days after the surgery. The discovered effect of MSC EVs is most likely associated with the suppression of the activity of the inflammatory process in the PCT area, which, in turn, was caused by a longer ingress of detritus with blood flow into the myocardium. The absence of statistically significant differences between changes in the myocardium of the left and right ventricles may indicate that both detritus from the surgical site and MSC EVs affect the heart spreading through the coronary artery system.

FP039AGEING OF MESENCHYMAL MULTIPOTENT STROMAL CELLS: PHYSIOLOGICAL ALTERATIONS AFFECTING THE THERAPEUTIC EFFICACY

2019 ◽  
Vol 34 (Supplement_1) ◽  
Author(s):  
Egor Plotnikov ◽  
Tatyana Danilina ◽  
Kirill Goryunov ◽  
Denis Silachev ◽  
Ljubava Zorova ◽  
...  
Keyword(s):  
Stromal Cells ◽  
Therapeutic Efficacy ◽  
Multipotent Stromal Cells ◽  
Physiological Alterations ◽  
Mesenchymal Multipotent Stromal Cells

scholarly journals Prospects of macacamulatta’s mesenchymal multipotent stromal cells usage for tissue -engineered scaffolds creation

2017 ◽  
Vol 12 (1) ◽  
Author(s):  
Elena Gubareva ◽  
Еlena Kuevda ◽  
Alexander Sotnichenko ◽  
Irina Gilevich ◽  
Ivan Gumenyuk ◽  
...  
Keyword(s):  
Stromal Cells ◽  
Multipotent Stromal Cells ◽  
Mesenchymal Multipotent Stromal Cells

Cytogenetic Characteristics of Diploid Lines of Mesenchymal Multipotent Stromal Cells

2021 ◽  
Vol 15 (6) ◽  
pp. 604-615
Author(s):  
V. A. Nikitina ◽  
T. A. Astrelina ◽  
I. V. Kobzeva ◽  
V. Yu. Nugis ◽  
E. E. Lomonosova ◽  
...  
Keyword(s):  
Stromal Cells ◽  
Multipotent Stromal Cells ◽  
Mesenchymal Multipotent Stromal Cells

scholarly journals Mesenchymal multipotent stromal cells and cancer safety: two sides of the same coin or a double-edged sword (review of foreign literature)

2021 ◽  
Vol 8 (1) ◽  
pp. 64-84
Author(s):  
D. A. Ivolgin ◽  
D. A. Kudlay
Keyword(s):  
Stromal Cells ◽  
Antitumor Agents ◽  
Epithelial Mesenchymal Transition ◽  
Chemotherapeutic Agents ◽  
Oncolytic Viruses ◽  
Antitumor Action ◽  
Malignant Neoplasms ◽  
Multipotent Stromal Cells ◽  
Mesenchymal Transition ◽  
Mesenchymal Multipotent Stromal Cells

Knowledge about the mechanisms of action of mesenchymal multipotent stromal cells (MSC) has undergone a significant evolution since their discovery. From the first attempts to use the remarkable properties of MSC in restoring the functions of organs and tissues, the most important question arose – how safe their use would be? One of the aspects of safety of the use of such biomaterial is tumorogenicity and oncogenicity. Numerous studies have shown that the mechanisms by which MSC realize their regenerative potential can, in principle, have a stimulating effect on tumor cells. This review presents specific mechanisms that have a potentially pro-tumor effect, which include the homing of MSC to the tumor site, support for replicative and proliferative signaling of both cancer cells and cancer stem cells, angiogenesis, and effects on the epithelial-mesenchymal transition. Along with pro-tumor mechanisms, the mechanisms of possible antitumor action are also described – direct suppression of tumor growth, loading and transportation of chemotherapeutic agents, oncolytic viruses, genetic modifications for targeting cancer, delivery of “suicide genes” to the tumor. Also, in conclusion, a small review of the current clinical trials of MSC as antitumor agents for malignant neoplasms of various localization (gastrointestinal tract, lungs, ovaries) is given. 

Sign in / Sign up

Export Citation Format

Share Document