scholarly journals Morphology of the Myocardium after Experimental Bone Tissue Trauma and the Use of Extracellular Vesicles Derived from Mesenchymal Multipotent Stromal Cells

2021 ◽  
Vol 11 (11) ◽  
pp. 1206
Author(s):  
Igor Maiborodin ◽  
Marina Klinnikova ◽  
Sergey Kuzkin ◽  
Vitalina Maiborodina ◽  
Sergey Krasil’nikov ◽  
...  

The effect of extracellular vesicles (EVs) of various origins on the heart structures in the time of health and disease has been well studied. At the same time, data on the distribution of EVs throughout the body after introduction into the tissues and the possibility of the influence of these EVs on organs distant from the injection site are practically absent. It is also necessary to note a certain inconsistency in the results of various researchers: from articles on the direct absorption of EVs derived from mesenchymal multipotent stromal cells (MSC EVs) by cardiomyocytes to the data that the heart is inherently immune to drug delivery mediated by nanoparticles. In this regard, the morphological changes in the myocardium of outbred rabbits of both sexes weighing 3–4 kg were studied at various times after experimental trauma of the bone tissue in the proximal condyle of the tibia (PCT) and the use of MSC EVs. As a result of modeling the PCT defect, rabbits develop myocardial edema in the heart muscle by the 3rd day, their lymphatic vessels expand, and then, on the 7th day, the blood vessels become dilated. In the myocardium, the relative and absolute contents of neutrophils, erythrocytes, and macrophages increase, but the percentage of lymphocytes decreases. By day 10, almost all of these changes return to their initial values. The detected transformations of the myocardium are most likely due to the ingress of detritus with the blood flow from the PCT. The use of MSC EVs to influence the regeneration of damaged tissue of PCT promotes earlier dilatation of the blood vessels of the heart with pronounced diapedesis of erythrocytes or even hemorrhages, prolongation of edema, the formation of blood clots in vessels with obliteration of their lumen, sclerotic transformation of vascular walls and paravascular tissues. In the myocardium, the number density of neutrophils, the percentage of lymphocytes, and neutrophils become smaller, with a simultaneous increase in the relative numbers of erythrocytes and macrophages, and changes in the content of macrophages remained until the end of the observation—up to 10 days after the surgery. The discovered effect of MSC EVs is most likely associated with the suppression of the activity of the inflammatory process in the PCT area, which, in turn, was caused by a longer ingress of detritus with blood flow into the myocardium. The absence of statistically significant differences between changes in the myocardium of the left and right ventricles may indicate that both detritus from the surgical site and MSC EVs affect the heart spreading through the coronary artery system.

2021 ◽  
Vol 22 (16) ◽  
pp. 8774
Author(s):  
Igor Maiborodin ◽  
Aleksandr Shevela ◽  
Vera Matveeva ◽  
Vitaly Morozov ◽  
Michael Toder ◽  
...  

Herein, the aim was to study the state of the bone tissue adjacent to dental implants after the use of extracellular vesicles derived from multipotent stromal cells (MSC EVs) of bone marrow origin in the experiment. In compliance with the rules of asepsis and antiseptics under general intravenous anesthesia with propofol, the screw dental implants were installed in the proximal condyles of the tibia of outbred rabbits without and with preliminary introduction of 19.2 μg MSC EVs into each bone tissue defect. In 3, 7, and 10 days after the operation, the density of bone tissue adjacent to different parts of the implant using an X-ray unit with densitometer was measured. In addition, the histological examinations of the bone site with the hole from the removed device and the soft tissues from the surface of the proximal tibial condyle in the area of intra-bone implants were made. It was found out that 3 days after implantation with the use of MSC EVs, the bone density was statistically significantly higher by 47.2% than after the same implantation, but without the injection of MSC EVs. It is possible that as a result of the immunomodulatory action of MSC EVs, the activity of inflammation decreases, and, respectively, the degree of vasodilation in bones and leukocyte infiltration of the soft tissues are lower, in comparison with the surgery performed in the control group. The bone fragments formed during implantation are mainly consolidated with each other and with the regenerating bone. Day 10 demonstrated that all animals with the use of MSC EVs had almost complete fusion of the screw device with the bone tissue, whereas after the operation without the application of MSC EVs, the heterogeneous histologic pattern was observed: From almost complete osseointegration of the implant to the absolute absence of contact between the foreign body and the new formed bone. Therefore, the use of MSC EVs during the introduction of dental implants into the proximal condyle of the tibia of rabbits contributes to an increase of the bone tissue density near the device after 3 days and to the achievement of consistently successful osseointegration of implants 10 days after the surgery.


2019 ◽  
Vol 6 (5) ◽  
pp. 55-67
Author(s):  
I. V. Maiborodin ◽  
S. V. Khomenyuk ◽  
T. V. Mikheeva ◽  
G. Yu. Yarin ◽  
V. I. Maiborodina ◽  
...  

Background. The scientific literature contents clearly not enough data at interaction of collagen materials with a living organism and about influence of multipotent stromal cells (MSC) on this process. There are controversies about the collagen degradation and the development of foreign body reactions. However, without account these results, it is impossible to estimate the timeframes for complete lysis of such materials, to progress effective methods for the prevention and treatment of developing complications. Objective. To study the features of the collagen-based material degradation after implantation with adsorbed autologous mesenchymal MSC of bone marrow origin (AMMSCBMO) in the experiment. Design and methods. In different times the condition of tissues around the implanted collagen membrane with adsorbed AMMSCBMO was studied by method of light microscopy. Results. The number of vessels and cellular elements in the collagen material implanted without AMMSCBMO increases to 3 weeks and remains at this level until the end of the observation. A distinctive features of the use of collagen membrane with adsorbed AMMSCBMO are increased vascularization and cellular infiltration of the material in the first 2 weeks after surgery. This effect further leads to a more rapid degradation of all implanted collagen, including its more dense parts, but does not prevent the formation of multinuclear macrophages with fused cytoplasm. Conclusion. As a result of more significant vascularization and cellular infiltration caused by AMMSCBMO adsorbed on the surface of the collagen material, to the 4th week the entire implant is full destroyed and absorbed, and dense fibrous connective tissue is formed in its place. For the implantation into the body, one should choose the most homogeneous collagen materials, without areas of different density, as slowly degrading fragments can cause the development of granulomatous inflammation and the failure of the implantation procedure.


2018 ◽  
Vol 6 (9) ◽  
Author(s):  
DR.MATHEW GEORGE ◽  
DR.LINCY JOSEPH ◽  
MRS.DEEPTHI MATHEW ◽  
ALISHA MARIA SHAJI ◽  
BIJI JOSEPH ◽  
...  

Blood pressure is the force of blood pushing against blood vessel walls as the heart pumps out blood, and high blood pressure, also called hypertension, is an increase in the amount of force that blood places on blood vessels as it moves through the body. Factors that can increase this force include higher blood volume due to extra fluid in the blood and blood vessels that are narrow, stiff, or clogged(1). High blood pressure can damage blood vessels in the kidneys, reducing their ability to work properly. When the force of blood flow is high, blood vessels stretch so blood flows more easily. Eventually, this stretching scars and weakens blood vessels throughout the body, including those in the kidneys.


2013 ◽  
Vol 154 (4) ◽  
pp. 537-543 ◽  
Author(s):  
E. G. Skurikhin ◽  
E. S. Khmelevskaya ◽  
O. V. Pershina ◽  
N. N. Ermakova ◽  
V. A. Krupin ◽  
...  

2010 ◽  
Vol 316 (15) ◽  
pp. 2447-2455 ◽  
Author(s):  
Egor Y. Plotnikov ◽  
Tatyana G. Khryapenkova ◽  
Svetlana I. Galkina ◽  
Gennady T. Sukhikh ◽  
Dmitry B. Zorov

Author(s):  
Md Zeeshan ◽  
Deshbandhu Joshi

The cardiovascular system refers to the heart, blood vessels and the blood. Blood contains oxygen and other nutrients which your body needs to survive. The body takes these essential nutrients from the blood. Angiography is an imaging test that uses X-rays to view your body's blood vessels. The X-rays provided by an angiography are called angiograms. This test is used to study narrow, blocked, enlarged, or malformed arteries or veins in many parts of your body, including your brain, heart, abdomen, and legs. Angioplasty is a procedure to restore blood flow through the artery. You have angioplasty in a hospital. The doctor threads a thin tube through a blood vessel in the arm or groin up to the involved site in the artery. The tube has a tiny balloon on the end. Cardiovascular disease generally refers to conditions that involve narrowed or blocked blood vessels that can lead to a heart attack, chest pain (angina) or stroke. Other heart conditions, such as those that affect your heart's muscle, valves or rhythm, also are considered forms of heart disease. Keyword: cardiovascular system, angiography, angioplasty


2010 ◽  
Vol 90 (7) ◽  
pp. 985-996 ◽  
Author(s):  
Ali H Amin ◽  
Zakaria Y Abd Elmageed ◽  
Devika Nair ◽  
Megan I Partyka ◽  
Philip J Kadowitz ◽  
...  

2010 ◽  
Vol 341 (1) ◽  
pp. 147-158 ◽  
Author(s):  
Julie A. Semon ◽  
Lauren H. Nagy ◽  
Claire B. Llamas ◽  
H. Alan Tucker ◽  
Ryang Hwa Lee ◽  
...  

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