SUGAR ACCUMULATION IN HIGHBUSH BLUEBERRY FRUIT AS AFFECTED BY ARTIFICIAL POLLINATION WITH DIFFERENT POLLEN SOURCES IN RELATION TO SEED NUMBER, INVERTASE ACTIVITIES AND ABA CONTENT

2002 ◽  
pp. 47-51 ◽  
Author(s):  
K. Kobashi ◽  
S. Sugaya ◽  
M. Fukushima ◽  
S. Iwahori
HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 784D-784
Author(s):  
Jorge B. Retamales ◽  
Cerardo A. Accedondo

Calcium gradients were established in firm (`Bluecrop' and `Blueray') and soft (`Ivanhoe') highbush blueberry (Vaccinium corymbosum L.) fruits from a 7-year-old planting at La Union, southern Chile. Manual firmness measurements established that `Ivanhoe' fruit was softer than either `Bluecrop' or `Blueray'. In all varieties, Ca concentrations were: seed > pulp > epidermis; opposite trends were found for K+Mg/Ca ratios. Seed number and Ca concentration in the pulp were negatively correlated in `Bluecrop' and `Ivanhoe', but not in `Blueray'. In a related experiment, the response of `Bluecrop' to preharvest sprays of two calcium sources (chloride and nitrate) in four doses (0, 47.5, 35, or 190 g Ca/100 liters of water) was studied; dose and source interactions were not significant. Both calcium sources affected fruit Ca concentrations similarly;l calcium applications, either as nitrate or chloride, increased Ca significantly in epidermis and seed; the highest dose was required to raise significantly Ca concentrations in the pulp. K+Mg/Ca ratios in nitrate- or chloride-treated fruit were: pulp > seed > epidermis.


Plants ◽  
2018 ◽  
Vol 7 (4) ◽  
pp. 96
Author(s):  
Kenichi Doi ◽  
Ryouichi Nozaki ◽  
Kouji Takahashi ◽  
Naoto Iwasaki

In present research, differences in the number of seeds per berry (NSB), berry fresh weight (BW), days to ripening from flowering (DRF), and the duration of a slow growth phase (DS II) among pollen sources were investigated in highbush blueberry (Vaccinium corymbosum). NSB, as well as BW and DRF, were significantly different among the pollen sources. Analysis of covariance (ANCOVA) with NSB as the covariate showed significant interaction between the NSB and pollen sources on BW and DRF when self-pollination was included. However, ANCOVA without self-pollination showed no significant effect of the pollen source on BW and DRF. On the other hand, DS II was negatively correlated with NSB, and no significant interaction between NSB and pollen sources was found, even though self-pollination was included. Although the relationship between NSB and DS II appeared not to be statistically influenced by the different pollen sources, there seemed to be some difference between self- and cross-pollination. DS II shortened as the NSB increased, which may have led to a decrease in DRF.


HortScience ◽  
2016 ◽  
Vol 51 (10) ◽  
pp. 1228-1232
Author(s):  
Lisa W. DeVetter ◽  
Sean Watkinson ◽  
Ramesh Sagili ◽  
Timothy Lawrence

Commercial production of northern highbush blueberry (Vaccinium corymbosum) is dependent upon rented colonies of honey bees (Apis mellifera) for meeting pollination requirements. Despite the prevalent use of honey bees, growers in Washington State and the greater Pacific Northwest (PNW), particularly those located in the western regions, claim pollination is limited and yield potential is subsequently reduced due to pollination deficits. However, there have been no studies or surveys that document this occurrence for this economically important region of blueberry production. The objective of this study was to survey honey bee activity in commercial plantings of ‘Duke’ highbush blueberry in western and eastern Washington and to assess the relationship between honey bee activity, growing region, and select yield components. Honey bee colony strength was also assessed to evaluate this variable’s relationship to honey bee activity and measured yield components. Sixteen and 18 commercial ‘Duke’ blueberry fields across Washington State were surveyed in 2014 and 2015, respectively. Average number of honey bee visitations per plant and honey bee colony strength were determined to evaluate overall honey bee activity. Estimated yield, berry number per plant, berry size (mass), and seed number per berry were also determined and analyzed to determine their relationship to honey bee activity through regression analysis. Honey bee visitation rates differed between western and eastern Washington, with western Washington sites consistently below recommended honey bee densities. Colony strength was also below recommended levels, but was lower for western Washington relative to eastern Washington. Estimated yield and berry number differed across sites and years, but were not related to honey bee visitation rates. Regression analysis revealed few significant relationships, although honey bee visitation rates were positively related to seed number per berry and seed number was positively related to berry size (R2 = 0.25 and 0.16, respectively). Berry size was also positively related to colony strength (R2 = 0.63). This study demonstrates that honey bee activity is limited in Washington blueberry production, particularly in western Washington, when compared with recommendations for optimal honey bee activity in blueberry. However, yields were unaffected between the compared regions. The lack of a relationship between honey bee visitation rates and yields suggests that pollination is sufficient for ‘Duke’ blueberry in Washington State and pollination deficits do not limit yield for this cultivar under the conditions of the study.


1997 ◽  
Vol 122 (6) ◽  
pp. 891-896 ◽  
Author(s):  
Kenna E. MacKenzie

The effects of pollination treatments on fruit set and five berry characteristics [mass, diameter, number of apparently viable seeds (well-developed, plump with dark seed coat), total seed number (includes apparently viable and partially developed seeds), and harvest date] were examined on three highbush blueberry cultivars. Pollination treatments included unpollinated, open pollinated, emasculated, and three hand pollinations that used pollen from the same flower, from the same cultivar, or from a different cultivar. Berries matured earliest and were smallest with the most apparently viable seeds in `Northland', `Patriot' had the greatest fruit set and smallest seed number, and `Bluecrop' matured the latest. Fruit set was greater, berry size larger, seed number smaller, and maturation later in 1990 than 1991. For all three cultivars, berries were generally smallest, latest maturing, and had the fewest seeds when pollination was prevented and were largest with the most seeds and earliest maturing in open visitation. Emasculation resulted in berries similar to those from unpollinated flowers. For berry characteristics, cross-pollination was of benefit for `Patriot' and possibly `Northland' but not `Bluecrop'. Thus, commercial highbush blueberry planting designs must be based on the pollination requirements of the particular cultivar. `Northland' berries almost always had seeds, while `Patriot' showed high levels and `Bluecrop' low levels of parthenocarpy.


2016 ◽  
Vol 26 (2) ◽  
pp. 213-219 ◽  
Author(s):  
Sarah K. Taber ◽  
James W. Olmstead

Cross-pollination has been associated with improved fruit set, weight, and shortened time to ripening in southern highbush blueberry [SHB (Vaccinium corymbosum interspecific hybrids)]. Because of this, growers commonly plant two or more cultivars in small blocks to facilitate cross-pollination. However, many SHB cultivars may vary in the degree of improvement in each parameter after cross-pollination. Understanding the impacts of cross-pollination on a particular cultivar is crucial to forming planting recommendations, particularly as growers begin to transition to fields designed for machine harvest where large solid blocks would increase the harvest efficiency. The objective of this study was to examine the effects of cross- and self-pollination among 13 commonly planted or newly released SHB cultivars. Cross-pollination typically improved fruit set, fruit weight, and seed number while decreasing the average days to harvest. Cross-pollinated fruit always weighed more than self-pollinated fruit from the same cultivar, which was highly correlated to seed number per fruit. Although there was variation for each trait, interplanting with another unrelated cultivar sharing a similar bloom time remains the best recommendation to ensure early, high yield among these SHB cultivars.


HortScience ◽  
1999 ◽  
Vol 34 (3) ◽  
pp. 537B-537
Author(s):  
Ingrith D. Martinez ◽  
P.M. Lyrene

Fruit set, fruit size, and seed production after hand pollination in a greenhouse were compared for southern highbush blueberry managed in two ways: a) 69 clones were allowed to go dormant and lose their leaves in the field before being dug and subjected to 1000 hours at 5 °C and b) 26 clones were kept growing in a greenhouse through fall and winter without leaf loss and without chilling, to induce flowering on plants that had mature leaves. On each plant in both management systems, some flowers were self-pollinated, some were cross-pollinated, and others had the styles removed before anthesis to prevent pollination. For >1000 flowers per pollination treatment on the deciduous plants, fruit set averaged 1% for no pollination, 46% for self-pollination, and 76% for cross-pollination. The corresponding values for the evergreen plants were 23%, 59%, and 81%. Parthenocarpic berries averaged 0.37 g/berry for deciduous plants and 1.01 g for evergreen plants. Both crossed and selfed berry weights averaged slightly higher for the evergreen plants than for the deciduous plants, but seed number per berry was much lower for the evergreen plants (12 seeds in crossed berries and four seeds in selfed berries) compared to deciduous plants (37 and 8). Southern highbush blueberry plants that flower without going dormant appear to have much higher parthenocarpic capabilities than those that flower after a dormant period.


HortScience ◽  
2019 ◽  
Vol 54 (10) ◽  
pp. 1728-1736
Author(s):  
Bernadine C. Strik ◽  
Amanda J. Vance

The relationship between individual berry weight and viable seed number of small- (<15 mm), medium- (15–19 mm), and large-diameter (>19 mm) berries was studied over 2 years in nine cultivars (Aurora, Bluecrop, Bluegold, Draper, Duke, Liberty, Legacy, Ozarkblue, and Reka) through their harvest seasons. Plants were grown with two different preplant amendment-mulch treatments, but this treatment had no effect on the variables measured, so data were pooled. The highest average seeds/berry was in ‘Bluecrop’ (55.5) and ‘Duke’ (50.0) and the lowest in ‘Bluegold’ (17.1), ‘Aurora’ (22.5), and ‘Liberty’ (23.5). Average berry weight over the fruiting season ranged from 1.79 and 1.80 g for ‘Liberty’ and ‘Reka’, respectively, to 2.30 and 2.44 g in ‘Ozarkblue’ and ‘Draper’, respectively. The average number of seeds/berry was higher in 2010 than in 2009 for all cultivars, ranging from 14% higher in ‘Reka’ to 96% higher in ‘Liberty’. The flowering period was earlier in 2010 than in 2009, but bloom was concentrated in 2009 (28 days) compared with 2010 (45 days), likely affecting pollinator success. In general, seeds/berry and berry weight declined through the harvest season. Some cultivars had a considerable difference in the number of seeds in large-sized than in small-sized berries (e.g., 89%, 107%, 108%, and 147% more seeds in ‘Aurora’, ‘Reka’, ‘Bluegold’, and ‘Liberty’, respectively), whereas others had relatively little difference (14% and 36% in ‘Draper’ and ‘Bluecrop’, respectively). There was a linear relationship between berry weight and viable seeds/berry. Cultivars did not differ in the berry weight per seed (slope of the line) between the 2 years, except for ‘Draper’ (only significant in 2009), ‘Legacy’, and ‘Reka’. ‘Bluegold’ had the greatest berry weight per seed and ‘Reka’ the lowest. The weakest relationship between berry weight and seed number was in ‘Draper’. Cultivars that produced parthenocarpic fruit of marketable size were ‘Aurora’, ‘Bluegold’, and ‘Liberty’. ‘Duke’, ‘Legacy’, ‘Reka’, and ‘Ozarkblue’ produced commercially acceptable fruit (0.75–1.0 g) with fewer than 7 seeds/berry. By contrast, some cultivars required a relatively large number of seeds to produce a berry including ‘Bluecrop’ (28–40 seeds), ‘Draper’ (15–23 seeds), and ‘Legacy’ in 2010 (20 seeds). The number of seeds per berry accounted for as much as 87% of the variability in berry weight indicating the importance of good pollination and seed set for berry weight and yield in these cultivars.


HortScience ◽  
2018 ◽  
Vol 53 (2) ◽  
pp. 191-194
Author(s):  
Matthew Arrington ◽  
Lisa Wasko DeVetter

Yield components including fruit set and berry size in northern highbush blueberry (Vaccinium corymbosum) can be limited in key production regions like western Washington. Climactic conditions influence the activity levels of blueberry’s primary commercial pollinator, honey bee (Apis mellifera). Cool springs with frequent rainfall, which are common during the spring bloom period in western Washington, can reduce honey bee activity, pollination efficiency, and subsequent fruit set and yields. Increasing honey bee hive density may be a simple technique that growers can employ to increase the number of honey bees foraging during periods of good weather, interspersed with the poor weather, and therefore, increase fruit set and related yield components. The objective of this study was to evaluate if increased honey bee hive densities improve pollination and subsequent yield components in western Washington blueberry. Three field sites with mature ‘Duke’ plants were stocked with 10 hives/ha of honey bees (control), and three other field sites (also ‘Duke’) were stocked with 20 hives/ha (high hive density). Honey bee visitation and yield components, including fruit set and berry weight, were measured. Estimated yield, seed number/berry, and fruit firmness were also monitored. There were no significant differences in fruit set regardless of honey bee hive density. However, honey bee visitation and estimated yield increased with increased honey bee hive density. Berry weight and seed number per berry were also increased with increased honey bee hive density, although firmness was unaffected. Results indicate that increasing honey bee hive densities can help blueberry growers improve berry size and overall yields, suggesting this is a practice growers can implement if their production is constrained by insufficient pollination.


1997 ◽  
Vol 122 (5) ◽  
pp. 625-629 ◽  
Author(s):  
Y.H. Huang ◽  
C.E. Johnson ◽  
G.A. Lang ◽  
M.D. Sundberg

Ovule abortion occurred between 5 and 10 days after pollination (DAP) in self- and cross-pollinated `Sharpblue' blueberry (Vaccinium corymbosum L.) fruit. More ovule abortion occurred with self-pollination (35%) than with cross-pollination (22% for `Sharpblue' × `O'Neal' and 29% for `Sharpblue' × `Gulfcoast'), and there were more poorly developed ovules with self-pollination (88.1%) than with cross-pollination (× `O'Neal', 33.6%; × `Gulfcoast' 50.8%). The increase in ovule area correlated exponentially with fruit growth during early developmental stages, regardless of pollination treatment. However, cross-pollination resulted in significantly greater ovule area and fruit mass during early fruit development as well as at ripening. Ovule area was maximum at 25 to 30 DAP for both pollination treatments, followed by exponential fruit growth (stage III). Cross-pollination resulted in greater fruit growth and a shorter stage III. At 10 DAP, ovules from cross-pollination were larger than those from self-pollination, suggesting that cross-pollination initiated ovule growth immediately after fertilization. This research suggests that southern highbush blueberry fruit growth and development is intimately associated with ovule growth and development, which is affected by pollen sources.


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