EFFECT OF FRUIT CRYOPRESERVATION ON IN VITRO GERMINATION OF ZYGOTIC EMBRYOS OF SEVERAL SPECIES OF ILEX

ABSTRACT The interspecific oil palm hybrid BRS Manicoré (E. guineensis x E. oleifera) has superior agronomic characteristics. However, the germination rate is low (30%) and the process is slow when the seeds are sown in a conventional form. The purpose of this study was to optimize the in vitro germination of zygotic embryos of this hybrid comparing seed lots. The viability of zygotic embryos was evaluated by the tetrazolium test (0.075%) for 4 h. The embryos were cultured on MS and Y3 culture media, with and without the addition of NaH2PO4, as well as on MS, MS1/2 and N6 medium. In MS medium containing NaH2PO4, the germination rate was increased from 40 to 70% in comparison with the medium without sodium phosphate. The comparison between the culture media MS, MS 1/2, N6 and Y3 showed that 75% of zygotic embryos cultured in the Y3 medium formed whole plants (with roots and shoots defined), a higher percentage than embryos cultured on MS, MS 1/2 and N6 media (46, 35 and 17% respectively). In the same Y3 culture medium, the embryos were larger (36% ≥ 2 cm and 30% ≥ 5 cm) than in the other media. Results obtained by the tetrazolium test were similar to those of germination, showing the effect of the genotype of each seed lot. For the germination and development of plantlets it is essential to add NaH2PO4 to a culture medium containing no phosphate or with a low phosphate concentration.

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GA3 induces expression of E2F-like genes and CDKA during in vitro germination of zygotic embryos of Cocos nucifera (L.)

Plant Cell Tissue and Organ Culture (PCTOC) ◽

10.1007/s11240-011-9996-5 ◽

2011 ◽

Vol 107 (3) ◽

pp. 461-470 ◽

Cited By ~ 4

Author(s):

Mayra Montero-Cortés ◽

Iván Cordova ◽

Jean-Luc Verdeil ◽

Valerie Hocher ◽

América Pech y Ake ◽

...

Keyword(s):

Cocos Nucifera ◽

Zygotic Embryos ◽

In Vitro Germination ◽

Cocos Nucifera L

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Culture of isolated zygotic embryos of Pinus radiata D. Don. Part I: Factors influencing in vitro germination and growth of isolated embryos

In Vitro Cellular & Developmental Biology - Plant ◽

10.1079/ivp2001260 ◽

2002 ◽

Vol 38 (2) ◽

pp. 191-197 ◽

Cited By ~ 2

Author(s):

Xueqin Lin ◽

David W. M. Leung

Keyword(s):

Pinus Radiata ◽

Zygotic Embryos ◽

In Vitro Germination ◽

Factors Influencing ◽

Germination And Growth

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In vitro germination of Abricó-de-Macaco (Couroupita guianensis aubl.)zygotic embryos in different culture media and light spectra

Ciência Rural ◽

10.1590/0103-8478cr20190906 ◽

2022 ◽

Vol 52 (1) ◽

Author(s):

Christian Aparecido Demétrio ◽

Jéssica Fernanda de Oliveira Jacob ◽

Guilherme Bovi Ambrosano ◽

Paulo Hercílio Viegas Rodrigues

Keyword(s):

Plant Growth ◽

Culture Media ◽

Zygotic Embryos ◽

In Vitro Germination ◽

Forest Species ◽

In Vitro Morphogenesis ◽

Light Spectra ◽

Couroupita Guianensis ◽

Secondary Roots

ABSTRACT: Couroupita guianensis Aubl. is an Amazonian forest species with important medicinal and ornamental value. This study evaluated the effect of different culture media and light spectra on the in vitro germination and development of the zygotic embryos of C. guianensis. The culture media, MS and WPM, were evaluated without the addition of plant growth regulators and were associated with four LED light spectra: white (CW), 70% red + 30% blue (R2B), 100% red (R), and 100% blue (B). One hundred percent of the seeds successfully underwent in vitro germination, and the culture media did not interfere with embryo development. In addition to this, the different light spectra induced in vitro morphogenesis and R2B treatment significantly promoted the production of secondary roots. This effect may aid in the rooting and acclimatization of seedlings of this species.

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