ASSESSMENT OF GENETIC VARIABILITY OF BAMBARA GROUNDNUT (VIGNA SUBTERRANEAN (L.) VERDC.) ACCESSIONS USING MORPHOLOGICAL TRAITS AND MOLECULAR MARKERS

2013 ◽  
pp. 779-790 ◽  
Author(s):  
O. Molosiwa ◽  
S.M. Basu ◽  
F. Stadler ◽  
S. Azam-Ali ◽  
S. Mayes
2021 ◽  
Vol 54 ◽  
pp. 37-46
Author(s):  
Hwan Woo Jung-Kim ◽  
Rosalba Mireya Hernández-Herrera ◽  
Ildefonso Enciso-Padilla ◽  
Martha Isabel Torres-Morán ◽  
Ana Paulina Velasco-Ramírez

Genetika ◽  
2005 ◽  
Vol 37 (2) ◽  
pp. 121-130 ◽  
Author(s):  
Dejana Saftic-Pankovic ◽  
Jovanka Atlagic ◽  
Tomka Miljanovic ◽  
Natasa Radovanovic

Genus Helianthus consists of 49 species. Two species H. giganteus L. and H. maximiliani Sch., distributed and collected in North America, were investigated. In order to determine morphological variability in/between these two species, fifteen populations of each species were used. Thirty traits were measured on five plants per species, grown in the same conditions in the wild species nursery at Rimski Sancevi. According to the investigated morphological traits, three species of H. giganteus were closer to H. maximiliani populations, which possibly indicates the existence of a new intraspecies taxon in H. giganteus. In order to test this hypothesis molecular variability of the same populations-species, was also investigated. The polymorphism of genomic DNA, that was isolated from frozen leaves, was investigated by microsatellites, recently shown to be the most powerful for the analysis of molecular genetic variability in genus Helianthus. Obtained results confirm the high variability between examined populations. Dendrograms constructed by cluster analysis of examined morphological traits and molecular markers are discussed.


2017 ◽  
Vol 23 (1) ◽  
Author(s):  
R.A. PATIL ◽  
S.G. BHARAD ◽  
S.N. SAWANT

Assessment of genetic diversity in the available germplasm is the prerequisite for development of improved genotypes through planned breeding programmes. In the view of this Forty-eight genotypes of seedling origin guava along with 1 check (L-49/Sardar) collected and conserved at germplasm block, Main Garden, Department of Horticulture, Dr. P. D. A. University, Akola were evaluated for genetic variability and diversity based on the qualitative characteristics. The genotypes were evaluated for sixteen morphological traitsviz. tree, leaf, floral and fruit traits. Results Show considerable extent of variability amongst the 49 genotypes in each traits. A sizeable amount of intrapopulation diversity recorded can be used to identify diverse parents which can be utilized in hybridization programmes.


2013 ◽  
Vol 56 (5) ◽  
pp. 408-420 ◽  
Author(s):  
Stefano Meneghetti ◽  
Angelo Costacurta ◽  
Luigi Bavaresco ◽  
Antonio Calo’

2017 ◽  
Vol 45 (2) ◽  
pp. 383-391 ◽  
Author(s):  
Constantinos SALIS ◽  
Ioannis E. PAPADAKIS ◽  
Spyridon KINTZIOS ◽  
Marianna HAGIDIMITRIOU

The behavior of six citrus rootstocks, Volkameriana, Citrumelo ‘Swingle’, Citrange ‘Carrizo’, Poncirus trifoliata ‘Serra’, Poncirus trifoliata ‘Rubidoux’ and Poncirus trifoliata ‘Flying Dragon’, in in vitro propagation was studied and compared for shoot proliferation and rooting. In addition, the genetic relationships among the rootstocks studied and other Citrus species, using the Inter-Simple Sequence Repeats (ISSR) molecular markers, were investigated. Nodal explants of three months old shoots were used in Murashige and Skoog medium supplemented with N6-benzyladenine (BA) for shoot proliferation and with naphthaleneacetic acid (NAA) for rooting. The rootstock Volkameriana showed a statistically significant higher number of shoots (1.81), shoot length (15.14 mm) and number of leaves per explant (5.81), while all three Poncirus trifoliata rootstocks showed the lowest numbers. The number of roots and root length per explant were evaluated at the end of the rooting phase. The rootstock ‘Swingle’ showed a higher number of roots per explant (4.2) followed by ‘Flying Dragon’ (3.93) and ‘Carrizo’ (3.23) rootstocks. The rootstocks ‘Swingle’ (140.8 mm), Volkameriana (148 mm) and ‘Flying Dragon’ (131.12 mm) had significantly higher root length per explant compared to ‘Carrizo’ (31 mm) and ‘Rubidoux’ (34.5 mm). The ISSR molecular marker technique used in the present study grouped successfully the different species, varieties and rootstocks studied, revealing their genetic variability. The genetic variability observed among the rootstocks ranged between 0.29 (Poncirus trifoliata ‘Serra’ and Citrumelo ‘Swingle’) and 0.60 (Volkameriana and Citrumelo ‘Swingle’). The response of the rootstocks studied in in vitro propagation however is not related to their genetic affinity.


2013 ◽  
Vol 43 (6) ◽  
pp. 978-984 ◽  
Author(s):  
Vanice Dias Oliveira ◽  
Allivia Rouse Carregosa Rabbani ◽  
Ana Veruska Cruz da Silva ◽  
Ana da Silva Lédo

This research had as objective to characterize genetically individuals of physic nut cultivated in experimental areas in Sergipe, Brazil by means of RAPD molecular markers. Leaves of 40 individuals were collected and DNA was isolated using CTAB 2% method. Were used 30 primers RAPD for DNA amplification, and this data was used to estimate the genetic similarity among the pairs of individuals, using Jaccard coefficient, and group them out for the UPGMA method. Also, the genetic structure and diversity of the populations were assessed using AMOVA. Of the 100 fragments generated, 29 of were polymorphic. A similarity average of 0.54 among the individuals was found and the amplitude similarities varied from 0.18 to 1.00. One of them (U5) was unit clusters and formed by the most divergent individuals. AMOVA indicated that there is more variation within (63%) the population. In conclusion, it was possible verify genetic variability in physic nut using RAPD markers at these experimental areas.


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