Reassessing the suitability of a monoclonal antibody for the generic serological detection of potyviruses

2021 ◽  
pp. 121-126
Author(s):  
W. Menzel ◽  
S. Winter
Keyword(s):  
Monoclonal Antibody ◽  
Serological Detection

scholarly journals Monoclonal antibody-based serological detection of potato virus M in potato plants and tubers

2020 ◽  
Vol 19 (5) ◽  
pp. 1283-1291 ◽  
Author(s):  
Yu ZHANG ◽  
Yan-ling GAO ◽  
Wan-qin HE ◽  
Ya-qin WANG ◽  
Ya-juan QIAN ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Potato Virus ◽  
Serological Detection ◽  
Potato Plants ◽  
Potato Virus M

scholarly journals Monoclonal Antibody-Based Serological Detection Methods for Wheat Dwarf Virus

2018 ◽  
Vol 33 (2) ◽  
pp. 173-180 ◽  
Author(s):  
Minghao Zhang ◽  
Rui Chen ◽  
Xueping Zhou ◽  
Jianxiang Wu
Keyword(s):  
Monoclonal Antibody ◽  
Dwarf Virus ◽  
Detection Methods ◽  
Wheat Dwarf Virus ◽  
Serological Detection

Preparation of Monoclonal Antibody against Ema-1 and Development of Rapid Serological Detection Method for Theileria equi Infection, Xinjiang, China

10.1645/19-98 ◽  
2020 ◽  
Vol 106 (2) ◽  
pp. 283
Author(s):  
Jingjing Song ◽  
Ruiqi Song ◽  
Panju Wang ◽  
Yang Zhang ◽  
Yan Yan ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Detection Method ◽  
Serological Detection ◽  
Theileria Equi

scholarly journals Monoclonal Antibody-Based Serological Detection of Rice Stripe Mosaic Virus Infection in Rice Plants or Leafhoppers

2019 ◽  
Vol 35 (2) ◽  
pp. 227-234 ◽  
Author(s):  
Liqian Guo ◽  
Jiayu Wu ◽  
Rui Chen ◽  
Jian Hong ◽  
Xueping Zhou ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Virus Infection ◽  
Mosaic Virus ◽  
Serological Detection ◽  
Rice Plants ◽  
Mosaic Virus Infection

scholarly journals Development of a Human-Murine Chimeric Immunoglobulin M for Use in the Serological Detection of Human Alphavirus Antibodies

2011 ◽  
Vol 18 (12) ◽  
pp. 2181-2182 ◽  
Author(s):  
Brett A. Thibodeaux ◽  
Nathan M. Liss ◽  
Amanda N. Panella ◽  
John T. Roehrig
Keyword(s):  
Monoclonal Antibody ◽  
Immunosorbent Assay ◽  
Immunoglobulin M ◽  
Murine Monoclonal Antibody ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serological Detection ◽  
Antibody Capture

ABSTRACTDiagnosis of human alphaviral infections relies on serological techniques, such as the immunoglobulin M antibody capture–enzyme-linked immunosorbent assay (MAC-ELISA). We have humanized the alphavirus broadly cross-reactive murine monoclonal antibody 1A4B-6 to create a reagent capable of replacing human positive sera in the MAC-ELISA for diagnosis of human alphaviral infections.

scholarly journals Monoclonal antibody-based serological detection of Citrus yellow vein clearing virus in citrus groves

2017 ◽  
Vol 16 (4) ◽  
pp. 884-891 ◽  
Author(s):  
Zhen LIU ◽  
Yuan-ji SUNZHU ◽  
Xue-ping ZHOU ◽  
Jian HONG ◽  
Jian-xiang WU
Keyword(s):  
Monoclonal Antibody ◽  
Yellow Vein ◽  
Serological Detection ◽  
Vein Clearing ◽  
Citrus Groves

A blocking ELISA using a monoclonal antibody for the serological detection of Mycoplasma hyopneumoniae

1994 ◽  
Vol 56 (3) ◽  
pp. 338-345 ◽  
Author(s):  
M.F. Le Potier ◽  
P. Abiven ◽  
M. Kobisch ◽  
D. Crevat ◽  
P. Desmettre
Keyword(s):  
Monoclonal Antibody ◽  
Mycoplasma Hyopneumoniae ◽  
Serological Detection ◽  
Blocking Elisa

A web-like network of type vi collagen in human skin is revealed by immuno-electron microscopy

1988 ◽  
Vol 46 ◽  
pp. 382-383
Author(s):  
Douglas R. Keene ◽  
Robert W. Glanville ◽  
Eva Engvall
Keyword(s):  
Electron Microscopy ◽  
Monoclonal Antibody ◽  
Human Skin ◽  
Basement Membranes ◽  
Primary Antibody ◽  
Fat Cells ◽  
Secondary Antibody ◽  
Type Vi Collagen ◽  
Dermal Matrix ◽  
Immuno Electron Microscopy

A mouse monoclonal antibody (5C6) prepared against human type VI collagen (1) has been used in this study to immunolocalize type VI collagen in human skin. The enbloc method used involves exposing whole tissue pieces to primary antibody and 5 nm gold conjugated secondary antibody before fixation, and has been described in detail elsewhere (2).Biopsies were taken from individuals ranging in age from neonate to 65 years old. By immuno-electron microscopy, type VI collagen is found to be distributed as a fine branching network closely associated with (but not attached to) banded collagen fibrils containing types I and III collagen (Fig. 1). It appears to enwrap fibers, to weave between individual fibrils within a fiber, and to span the distance separating fibers, creating a “web-like network” which entraps fibers within deep papillary and reticular dermal layers (Fig. 2). Relative to that in the dermal matrix, the concentration of type VI collagen is higher around endothelial basement membranes limiting the outer boundaries of nerves, capillaries, and fat cells (Fig. 3).

High-voltage electron microscopy of subretinal scar formation

1992 ◽  
Vol 50 (1) ◽  
pp. 486-487
Author(s):  
G.E. Korte ◽  
M. Marko ◽  
G. Hageman
Keyword(s):  
Electron Microscopy ◽  
Monoclonal Antibody ◽  
Retinal Pigment Epithelium ◽  
Glial Cells ◽  
High Voltage ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Sodium Iodate ◽  
High Voltage Electron Microscopy ◽  
Voltage Electron

Sodium iodate iv. damages the retinal pigment epithelium (RPE) in rabbits. Where RPE does not regenerate (e.g., 1,2) Muller glial cells (MC) forma subretinal scar that replaces RPE. The MC response was studied by HVEM in 3D computer reconstructions of serial thick sections, made using the STEREC0N program (3), and the HVEM at the NYS Dept. of Health in Albany, NY. Tissue was processed for HVEM or immunofluorescence localization of a monoclonal antibody recognizing MG microvilli (4).

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