Preparation of Monoclonal Antibody against Ema-1 and Development of Rapid Serological Detection Method for Theileria equi Infection, Xinjiang, China

10.1645/19-98 ◽  
2020 ◽  
Vol 106 (2) ◽  
pp. 283
Author(s):  
Jingjing Song ◽  
Ruiqi Song ◽  
Panju Wang ◽  
Yang Zhang ◽  
Yan Yan ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Detection Method ◽  
Serological Detection ◽  
Theileria Equi

scholarly journals Monoclonal antibody-based serological detection of potato virus M in potato plants and tubers

2020 ◽  
Vol 19 (5) ◽  
pp. 1283-1291 ◽  
Author(s):  
Yu ZHANG ◽  
Yan-ling GAO ◽  
Wan-qin HE ◽  
Ya-qin WANG ◽  
Ya-juan QIAN ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Potato Virus ◽  
Serological Detection ◽  
Potato Plants ◽  
Potato Virus M

scholarly journals Monoclonal Antibody-Based Serological Detection Methods for Wheat Dwarf Virus

2018 ◽  
Vol 33 (2) ◽  
pp. 173-180 ◽  
Author(s):  
Minghao Zhang ◽  
Rui Chen ◽  
Xueping Zhou ◽  
Jianxiang Wu
Keyword(s):  
Monoclonal Antibody ◽  
Dwarf Virus ◽  
Detection Methods ◽  
Wheat Dwarf Virus ◽  
Serological Detection

scholarly journals Detection of Antigenemia by Enzyme-Linked Immunosorbent Assay in Horses with Experimental Ehrlichia Risticii Infection

1993 ◽  
Vol 5 (1) ◽  
pp. 33-36 ◽  
Author(s):  
Richard E. Corstvet ◽  
Stephen D. Gaunt ◽  
Phillip A. Karns ◽  
David Burgermeister ◽  
Jere W. McBride ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Immunosorbent Assay ◽  
Detection Method ◽  
Serum Antibody ◽  
Clinical Signs ◽  
Clinical Findings ◽  
Antigen Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Infected Horse ◽  
Ehrlichia Risticii

Four horses were inoculated with Ehrlichia risticii contained in either infected murine P388 D1 cells or heparinized blood from an infected horse. All 4 horses produced serum antibody, plasma antigen, and clinical signs of the disease. An enzyme-linked immunosorbent assay was used to detect antibody in the serum and was also used in conjunction with an anti- E. risticii monoclonal antibody to detect antigenemia. These laboratory and clinical findings were correlated to determine the efficiency of the antigen detection method for discerning E. risticii infection.

A Rapid Serological Detection Method for Squamous Cell Carcinoma

1996 ◽  
Vol 22 (6) ◽  
pp. 575-583
Author(s):  
Hiroyuki Yoshizawa ◽  
Susumu Yamauchi ◽  
Takiko Kenjo ◽  
Yoshio Kuroshima ◽  
Tsunehisa Makino
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Detection Method ◽  
Serological Detection

scholarly journals Luminescent detection method for immunodot, Western, and Southern blots.

1986 ◽  
Vol 34 (12) ◽  
pp. 1645-1650 ◽  
Author(s):  
M M Leong ◽  
C Milstein ◽  
R Pannell
Keyword(s):  
Monoclonal Antibody ◽  
Cell Line ◽  
Indirect Method ◽  
Detection Method ◽  
Specific Activity ◽  
High Specific Activity ◽  
The Other ◽  
X Ray ◽  
Bispecific Monoclonal Antibody ◽  
Direct Binding

An anti-peroxidase-anti-biotin hybrid hybridoma rat cell line, capable of producing a bispecific monoclonal antibody, has been derived to explore its use in conjunction with a luminol immunodetection system. Luminescence was detected using x-ray film. The method was sufficiently sensitive and effective, but was less sensitive than autoradiographic methods using high-specific-activity 32P-labeled probes. Exposure times, on the other hand, were of the order of seconds rather than days. The direct binding of both peroxidase and biotin by the bispecific monoclonal antibody is simpler but less sensitive than the more conventional indirect method using a commercial peroxidase coupled with anti-rat antibody as a developing antibody.

scholarly journals Waveguide-Based Fluorescent Immunosensor for the Simultaneous Detection of Carbofuran and 3-Hydroxy-Carbofuran

Biosensors ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 191
Author(s):  
Weiming Sun ◽  
Lanhua Liu ◽  
Abdul Ghaffar Memon ◽  
Xiaohong Zhou ◽  
Hongwei Zhao
Keyword(s):  
Water Quality ◽  
Monoclonal Antibody ◽  
Human Health ◽  
Broad Spectrum ◽  
Rapid Detection ◽  
Detection Method ◽  
Simultaneous Detection ◽  
Agricultural Products ◽  
Linear Detection ◽  
High Binding Affinity

Carbofuran (CBF) is an efficient and broad-spectrum insecticide. As testing indicators for water quality and agricultural products, CBF and its metabolite 3-hydroxy-carbofuran (3-OH-CBF) are regulated by many countries. The detection of CBF and 3-OH-CBF is of great importance for the environment and human health. However, an immunosensor detection method for the simultaneous analysis of CBF and 3-OH-CBF remains unavailable. Herein, we report a waveguide-based fluorescent immunosensor for detecting CBF and 3-OH-CBF, synchronously. The immunosensor is based on a broad-spectrum monoclonal antibody with high binding affinity against CBF and 3-OH-CBF. The linear detection ranges for CBF and 3-OH-CBF are 0.29–2.69 and 0.12–4.59 μg/L, with limits of detection of 0.13 μg/L for CBF and 0.06 μg/L for 3-OH-CBF, respectively. The whole detection process for each cycle is approximately 30 min. The results show a good application prospect for the rapid detection of CBF and 3-OH-CBF in water or agricultural products.

scholarly journals Monoclonal Antibody-Based Serological Detection of Rice Stripe Mosaic Virus Infection in Rice Plants or Leafhoppers

2019 ◽  
Vol 35 (2) ◽  
pp. 227-234 ◽  
Author(s):  
Liqian Guo ◽  
Jiayu Wu ◽  
Rui Chen ◽  
Jian Hong ◽  
Xueping Zhou ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Virus Infection ◽  
Mosaic Virus ◽  
Serological Detection ◽  
Rice Plants ◽  
Mosaic Virus Infection

scholarly journals Development of a fluorescent immunochromatographic assay based on quantum dots for the detection of fleroxacin

RSC Advances ◽  
2021 ◽  
Vol 11 (36) ◽  
pp. 22005-22013
Author(s):  
Qingbao Yang ◽  
Yanhua Qi ◽  
Jingming Zhou ◽  
Yumei Chen ◽  
Chao Liang ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Quantum Dots ◽  
Fluorescent Probe ◽  
Rapid Detection ◽  
Detection Method ◽  
Water Soluble ◽  
Fluorescent Detection ◽  
Immunochromatographic Assay

(1) Water-soluble CdSe/ZnS QDs and an anti-FLE monoclonal antibody were used to prepare a fluorescent probe. (2) Primary rapid detection of FLE residues with visual fluorescent detection method.

scholarly journals Development of a Human-Murine Chimeric Immunoglobulin M for Use in the Serological Detection of Human Alphavirus Antibodies

2011 ◽  
Vol 18 (12) ◽  
pp. 2181-2182 ◽  
Author(s):  
Brett A. Thibodeaux ◽  
Nathan M. Liss ◽  
Amanda N. Panella ◽  
John T. Roehrig
Keyword(s):  
Monoclonal Antibody ◽  
Immunosorbent Assay ◽  
Immunoglobulin M ◽  
Murine Monoclonal Antibody ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serological Detection ◽  
Antibody Capture

ABSTRACTDiagnosis of human alphaviral infections relies on serological techniques, such as the immunoglobulin M antibody capture–enzyme-linked immunosorbent assay (MAC-ELISA). We have humanized the alphavirus broadly cross-reactive murine monoclonal antibody 1A4B-6 to create a reagent capable of replacing human positive sera in the MAC-ELISA for diagnosis of human alphaviral infections.

Sign in / Sign up

Export Citation Format

Share Document