scholarly journals Phylogenetic analysis of coat protein gene of tomato mosaic virus isolates circulating in Ukraine

2019 ◽  
Vol 77 (1) ◽  
pp. 44-50
Author(s):  
I. Pozhylov ◽  
T. Rudnieva ◽  
T. Shevchenko ◽  
O. Shevchenko ◽  
V. Tsvigun
Keyword(s):  
Phylogenetic Analysis ◽  
Coat Protein ◽  
Mosaic Virus ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Tomato Mosaic Virus ◽  
Chinese Isolate ◽  
Plant Host ◽  
High Level ◽  
Virus Isolates

Tomato mosaic virus (ToMV) induces highly infectious disease of vegetables, whereas use of virus-contaminated seed may lead to complete yield loss. This work was aimed at studying phylogenetic relationships of Ukrainian tomato isolates of ToMV with its known isolates by comparing nucleotide sequence of coat protein gene. ELISA, TEM, RT-PCR, sequence analysis using MEGA 5 software, and statistical methods. cDNAs of two novel Ukrainian isolates ToMV-ukr-5 and ToMV-ukr-10 corresponding to coat protein (CP) gene were sequenced and compared with other published ToMV sequences. On the constructed phylogenetic tree, ToMV isolates were grouped into two separate clusters. In addition to novel Ukrainian isolates ToMV-ukr-5 and ToMV-ukr-10, the first and larger cluster contained nearly all virus isolates used in this study with high (96-98,9 %) level of homology to Ukrainian isolates. The larger cluster was clearly separated into two subclusters: one grouping isolates with over 96,7 % identity with Ukranian isolates, and the other containing three strains and isolates with 96,1 % identity (tomato isolate SL-1, strain camellia isolated from a decorative plant, and isolate Dahlemense DSMZ PV-0135).Two novel Ukrainian isolates ToMV-ukr-5 and ToMV-ukr-10 have been isolated from tomato plants cultivated in open field conditions in different regions of Ukraine. Phylogenetic analysis confirmed high identity of Ukrainian isolates between themselves and with other published ToMV sequences. Ukrainian isolates were most homologous (>98 %) to Brazilian isolate Hemerocallis, to Chinese isolate G2, and to the following tomato isolates: AH4, Queensland, ToMV-tom and Ls-K, S14, and FERA_160205. The high level of homology was traced independently of the source of virus isolation, its plant host and their geography.

scholarly journals Presence and molecular characterization of alfalfa mosaic virus on tobacco in Serbia

2011 ◽  
Vol 26 (3) ◽  
pp. 229-243 ◽  
Author(s):  
Ivana Stankovic ◽  
Ana Vucurovic ◽  
Aleksandra Bulajic ◽  
Danijela Ristic ◽  
Janos Berenji ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Coat Protein ◽  
Mosaic Virus ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Rna Extraction ◽  
Alfalfa Mosaic Virus ◽  
Lower Percentage ◽  
Rt Pcr ◽  
Plant Host

Three-year investigation of the presence and distribution of tobacco viruses in Serbia revealed that Alfalfa mosaic virus (AMV) appeared every year with different frequency in tobacco crops. During 2008, the presence of AMV was detected in most of the tested samples (58.82%) and it was the second most common compared to all other viruses which presence was confirmed in Serbia. In 2006 and 2007, AMV was detected in a significantly lower percentage (2.80% and 13.64%, respectively). This study showed that Alfalfa mosaic virus was more commonly found in multiple infections with two, three or even four detected viruses. Single infections were detected only in 2006, in one tobacco field in the locality of Futog. During this investigation, a rapid and simple protocol was optimized and developed for molecular detection of AMV in tobacco leaves, using primers CPAMV1/CPAMV2 and commercially available kits for total RNA extraction as well as for RT-PCR (reverse transcription - polymerase chain reaction). Using RT-PCR and these primers that flank the AMV coat protein gene, a DNA fragment of 751 bp was amplified, sequenced, and compared with the sequences available in GenBank database. The sequence of isolate 196-08 (GenBank Acc. No. FJ527749) proved to be identical at the nucleotide level of 99 to 93% with those from other parts of the world. Phylogenetic analysis of 27 isolates based on 528 bp sequences of the coat protein gene did not show correlation of the isolates with their geographic origin or plant host and showed that these isolates fall into four molecular groups of strains. Serbian AMV isolate from tobacco belongs to group IV, the group that includes most of the isolates selected for phylogenetic analysis.

scholarly journals Sequence variability in the coat protein gene of Cowpea severe mosaic virus isolates from northeastern Brazil

2011 ◽  
Vol 36 (2) ◽  
pp. 121-124 ◽  
Author(s):  
José Evando A. Beserra Jr. ◽  
Eduardo C. Andrade ◽  
Rosa F.R. Araújo Camarço ◽  
Aline K.Q. Nascimento ◽  
José Albérsio A. Lima
Keyword(s):  
Coat Protein ◽  
Mosaic Virus ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Northeastern Brazil ◽  
Sequence Variability ◽  
Virus Isolates

scholarly journals Analysis of synonymous codon usage bias and phylogeny of coat protein gene in banana bract mosaic virus isolates

VirusDisease ◽  
2017 ◽  
Vol 28 (2) ◽  
pp. 156-163 ◽  
Author(s):  
Atul B. Patil ◽  
Vijayendra S. Dalvi ◽  
Akhilesh A. Mishra ◽  
Bal Krishna ◽  
Abdul Azeez
Keyword(s):  
Coat Protein ◽  
Codon Usage ◽  
Mosaic Virus ◽  
Coat Protein Gene ◽  
Codon Usage Bias ◽  
Protein Gene ◽  
Synonymous Codon ◽  
Synonymous Codon Usage ◽  
Virus Isolates ◽  
Synonymous Codon Usage Bias

scholarly journals Genetic variability and evolutionary analyses of the coat protein gene of Tomato mosaic virus

Virus Genes ◽  
2011 ◽  
Vol 43 (3) ◽  
pp. 435-438 ◽  
Author(s):  
E. A. Rangel ◽  
A. Alfaro-Fernández ◽  
M. I. Font-San-Ambrosio ◽  
M. Luis-Arteaga ◽  
L. Rubio
Keyword(s):  
Coat Protein ◽  
Genetic Variability ◽  
Mosaic Virus ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Tomato Mosaic Virus

scholarly journals Characterization of Coat Protein Gene of Cucumber Mosaic Virus Isolates in Iran

2013 ◽  
Vol 11 (2) ◽  
pp. x-x ◽  
Author(s):  
Nazanin Arafati ◽  
Shirin Farzadfar ◽  
Reza Pourrahim
Keyword(s):  
Coat Protein ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Virus Isolates

Characterization of the Coat Protein Gene of Cymbidium mosaic virus Isolates from India

2006 ◽  
Vol 154 (5) ◽  
pp. 275-280 ◽  
Author(s):  
A. R. Sherpa ◽  
V. Hallan ◽  
P. Pathak ◽  
A. A. Zaidi
Keyword(s):  
Coat Protein ◽  
Mosaic Virus ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Cymbidium Mosaic Virus ◽  
Virus Isolates

scholarly journals Inheritance of Resistance to Squash Mosaic Virus in a Squash Transformed with the Coat Protein Gene of Pathotype 1

HortScience ◽  
2002 ◽  
Vol 37 (3) ◽  
pp. 575-577 ◽  
Author(s):  
R. Provvidenti ◽  
David M. Tricoli
Keyword(s):  
New York ◽  
Coat Protein ◽  
Mosaic Virus ◽  
Coat Protein Gene ◽  
Cucurbita Pepo ◽  
Protein Gene ◽  
Recessive Gene ◽  
Summer Squash ◽  
American Strain ◽  
High Level

In a yellow summer squash (Cucurbita pepo L.) experimental line developed by Seminis Vegetable Seeds, the coat protein gene of an American strain of squash mosaic virus (SqMV-M88), conferred resistance to Arizona, California, New Jersey, and New York strains belonging to the two pathotypes of the virus. An analysis of genetic populations derived from crosses and reciprocal backcrosses of a homozygous SqMV-resistant line A127-1-2 with the susceptible cultivar Butterbar revealed that the high level of resistance mimics the response of a single recessive gene.

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