scholarly journals Presence and molecular characterization of alfalfa mosaic virus on tobacco in Serbia

2011 ◽  
Vol 26 (3) ◽  
pp. 229-243 ◽  
Author(s):  
Ivana Stankovic ◽  
Ana Vucurovic ◽  
Aleksandra Bulajic ◽  
Danijela Ristic ◽  
Janos Berenji ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Coat Protein ◽  
Mosaic Virus ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Rna Extraction ◽  
Alfalfa Mosaic Virus ◽  
Lower Percentage ◽  
Rt Pcr ◽  
Plant Host

Three-year investigation of the presence and distribution of tobacco viruses in Serbia revealed that Alfalfa mosaic virus (AMV) appeared every year with different frequency in tobacco crops. During 2008, the presence of AMV was detected in most of the tested samples (58.82%) and it was the second most common compared to all other viruses which presence was confirmed in Serbia. In 2006 and 2007, AMV was detected in a significantly lower percentage (2.80% and 13.64%, respectively). This study showed that Alfalfa mosaic virus was more commonly found in multiple infections with two, three or even four detected viruses. Single infections were detected only in 2006, in one tobacco field in the locality of Futog. During this investigation, a rapid and simple protocol was optimized and developed for molecular detection of AMV in tobacco leaves, using primers CPAMV1/CPAMV2 and commercially available kits for total RNA extraction as well as for RT-PCR (reverse transcription - polymerase chain reaction). Using RT-PCR and these primers that flank the AMV coat protein gene, a DNA fragment of 751 bp was amplified, sequenced, and compared with the sequences available in GenBank database. The sequence of isolate 196-08 (GenBank Acc. No. FJ527749) proved to be identical at the nucleotide level of 99 to 93% with those from other parts of the world. Phylogenetic analysis of 27 isolates based on 528 bp sequences of the coat protein gene did not show correlation of the isolates with their geographic origin or plant host and showed that these isolates fall into four molecular groups of strains. Serbian AMV isolate from tobacco belongs to group IV, the group that includes most of the isolates selected for phylogenetic analysis.

scholarly journals Phylogenetic analysis of coat protein gene of tomato mosaic virus isolates circulating in Ukraine

2019 ◽  
Vol 77 (1) ◽  
pp. 44-50
Author(s):  
I. Pozhylov ◽  
T. Rudnieva ◽  
T. Shevchenko ◽  
O. Shevchenko ◽  
V. Tsvigun
Keyword(s):  
Phylogenetic Analysis ◽  
Coat Protein ◽  
Mosaic Virus ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Tomato Mosaic Virus ◽  
Chinese Isolate ◽  
Plant Host ◽  
High Level ◽  
Virus Isolates

Tomato mosaic virus (ToMV) induces highly infectious disease of vegetables, whereas use of virus-contaminated seed may lead to complete yield loss. This work was aimed at studying phylogenetic relationships of Ukrainian tomato isolates of ToMV with its known isolates by comparing nucleotide sequence of coat protein gene. ELISA, TEM, RT-PCR, sequence analysis using MEGA 5 software, and statistical methods. cDNAs of two novel Ukrainian isolates ToMV-ukr-5 and ToMV-ukr-10 corresponding to coat protein (CP) gene were sequenced and compared with other published ToMV sequences. On the constructed phylogenetic tree, ToMV isolates were grouped into two separate clusters. In addition to novel Ukrainian isolates ToMV-ukr-5 and ToMV-ukr-10, the first and larger cluster contained nearly all virus isolates used in this study with high (96-98,9 %) level of homology to Ukrainian isolates. The larger cluster was clearly separated into two subclusters: one grouping isolates with over 96,7 % identity with Ukranian isolates, and the other containing three strains and isolates with 96,1 % identity (tomato isolate SL-1, strain camellia isolated from a decorative plant, and isolate Dahlemense DSMZ PV-0135).Two novel Ukrainian isolates ToMV-ukr-5 and ToMV-ukr-10 have been isolated from tomato plants cultivated in open field conditions in different regions of Ukraine. Phylogenetic analysis confirmed high identity of Ukrainian isolates between themselves and with other published ToMV sequences. Ukrainian isolates were most homologous (>98 %) to Brazilian isolate Hemerocallis, to Chinese isolate G2, and to the following tomato isolates: AH4, Queensland, ToMV-tom and Ls-K, S14, and FERA_160205. The high level of homology was traced independently of the source of virus isolation, its plant host and their geography.

Role of alfalfa mosaic virus coat protein gene in symptom formation

Virology ◽  
1991 ◽  
Vol 181 (2) ◽  
pp. 687-693 ◽  
Author(s):  
Lyda Neeleman ◽  
Antoinette C. Van Der Kuyl ◽  
John F. Bol
Keyword(s):  
Coat Protein ◽  
Mosaic Virus ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Alfalfa Mosaic Virus ◽  
Virus Coat Protein ◽  
Virus Coat ◽  
Symptom Formation

The sense and antisense coat protein gene of alfalfa mosaic virus strain N20 confers protection in transgenic tobacco plants

1997 ◽  
Vol 48 (4) ◽  
pp. 503 ◽  
Author(s):  
K. W. Jayasena ◽  
B. J. Ingham ◽  
M. R. Hajimorad ◽  
J. W. Randles
Keyword(s):  
Coat Protein ◽  
Mosaic Virus ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Alfalfa Mosaic Virus ◽  
35S Promoter ◽  
Pasture Legumes ◽  
Nicotiana Tabacum L ◽  
Sense Orientation ◽  
A Minor

The coat protein gene of a South Australian strain of alfalfa mosaic virus (AMV-N20 [NcS]) has been cloned, sequenced, and transferred into Nicotiana tabacum L. cv. Xanthi via Agrobacterium tumefaciens under the control of the CaMV 35S promoter. A number of lines (T0 generation) were selected with the coat protein gene either in sense orientation (CP+) or in antisense orientation (CP–). The T0 plants were tested for their gene expression and susceptibility to the homologous AMV strain. A significant delay in the onset of symptoms and a reduction in virus accumulation was observed in CP+ plants mechanically inoculated with AMV. CP– plants were also significantly protected but less so than the CP+ plants. Plants transformed with the expression vector only (CP0) showed a minor resistance to local infection on inoculated leaves compared with untransformed plants. The strategy of coat protein mediated protection (CPMP) using the CP gene in either messenger sense or antisense would therefore be appropriate for testing on economically important pasture legumes.

Resistance to Alfalfa mosaic virus in transgenic barrel medic lines containing the virus coat protein gene

2001 ◽  
Vol 52 (1) ◽  
pp. 67 ◽  
Author(s):  
K. W. Jayasena ◽  
M. R. Hajimorad ◽  
E. G. Law ◽  
A-U. Rehman ◽  
K. E. Nolan ◽  
...  
Keyword(s):  
Coat Protein ◽  
Mosaic Virus ◽  
Medicago Truncatula ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Single Gene ◽  
Alfalfa Mosaic Virus ◽  
Systemic Infection ◽  
Gene Copy ◽  
Sense Orientation

Medicago truncatula is used as a pasture legume and a source of nitrogen for grain crops in southern Australia. Alfalfa mosaic virus (AMV) infection reduces herbage production and nodulation. The coat protein gene of a South Australian strain of AMV (AMV N20) has been transferred to Medicago truncatula cv. Jemalong 2HA using Agrobacterium-mediated transformation. The most detailed investigations were carried out with the coat protein gene in the sense orientation (CP+). Progeny (T1, T2, T3) raised from self-pollinated primary transformants (T0) containing the coat protein CP+ gene were resistant to AMV. Based on Southern analysis and segregation, the transformants contained a single gene copy. In the T3 generation, one line was immune and one line showed resistance to AMV N20. The immune line contained no detectable virus when plant sap from either inoculated or systemic leaves was bioassayed on Phaseolus vulgaris. This line was also immune to the heterologous AMV S40 isolate. A line with the coat protein gene in antisense orientation (CP–) showed delayed systemic infection but was not immune. We conclude that coat protein mediated protection (CPMP) is an effective strategy for controlling AMV infection and should be further evaluated in the field.

scholarly journals Expression of alfalfa mosaic virus coat protein gene confers cross-protection in transgenic tobacco and tomato plants

1987 ◽  
Vol 6 (5) ◽  
pp. 1181-1188 ◽  
Author(s):  
Nilgun E. Tumer ◽  
Keith M. O'Connell ◽  
Richard S. Nelson ◽  
Patricia R. Sanders ◽  
Roger N. Beachy ◽  
...  
Keyword(s):  
Coat Protein ◽  
Mosaic Virus ◽  
Transgenic Tobacco ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Alfalfa Mosaic Virus ◽  
Cross Protection ◽  
Tomato Plants ◽  
Virus Coat Protein ◽  
Virus Coat

Plants Transformed with a Mutant Alfalfa Mosaic Virus Coat Protein Gene Are Resistant to the Mutant but Not to Wild-Type Virus

Virology ◽  
1994 ◽  
Vol 203 (2) ◽  
pp. 269-276 ◽  
Author(s):  
Peter E.M. Taschner ◽  
Guido Van Marle ◽  
Frans Th. Brederode ◽  
Nilgun E. Tumer ◽  
John F. Bol
Keyword(s):  
Coat Protein ◽  
Mosaic Virus ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Alfalfa Mosaic Virus ◽  
Wild Type Virus ◽  
Type Virus ◽  
Wild Type ◽  
Virus Coat Protein ◽  
Virus Coat

Resistance to Alfalfa Mosaic Virus in Transgenic Burley Tobaccos Expressing the AMV Coat Protein Gene

Crop Science ◽  
1998 ◽  
Vol 38 (6) ◽  
pp. 1661-1668 ◽  
Author(s):  
Dongmei Xu ◽  
Glenn B. Collins ◽  
Arthur G. Hunt ◽  
Mark T. Nielsen
Keyword(s):  
Coat Protein ◽  
Mosaic Virus ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Alfalfa Mosaic Virus

scholarly journals IDENTIFIKASI TOMATO INFECTIOUS CHLOROSIS VIRUS PENYEBAB PENYAKIT KLOROSIS PADA TANAMAN TOMAT DI CIPANAS JAWA BARAT MELALUI PERUNUTAN NUKLEOTIDA GEN PROTEIN SELUBUNG UTAMA

2015 ◽  
Vol 15 (1) ◽  
pp. 33
Author(s):  
Fitrianingrum Kurniawati ◽  
Gede Suastika ◽  
Giyanto .
Keyword(s):  
Coat Protein ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Rna Extraction ◽  
Dna Amplification ◽  
Causal Agent ◽  
Rt Pcr ◽  
Cdna Synthesis ◽  
Polymerase Chain ◽  
Tomato Infectious Chlorosis Virus

Identification of tomato infectious chlorosis virus, the causal agent of chlorosis disease on tomato in Cipanas West Java by sequencing of main coat protein gene nucleotide. Tomato infectious chlorosis virus (TICV) causes chlorosis on tomato. Tomatoes infected by this virus shows interveinal yellowing, necrotic, bronzing, brittleness, and declining in productivity. This study aims to identify the causal agent of chlorotic disease on tomato by sequencing the coat protein gene. The methods involve collecting infected plants, total RNA extraction, cDNA synthesis, DNA amplification, visualization of the results of reverse transcription polymerase chain reaction (PCR), and phylogenetic analysis using BLAST, clustal w, Bioedit v 7.0.5.3, MEGA v 6:06. RT-PCR using spesific primers (CP-F TICV Bam and TICV R-Hind) amplified a DNA band of 792 bp, which has been successfully sequenced and identified as TICV. Nucleotide sequences homology analysis showed that TICV Indonesia_TWJ isolate Cipanas is the same strain as TICV from other countries (99.4 – 100%), such as Spain, Greece, USA, France, and Italy.

Sign in / Sign up

Export Citation Format

Share Document