scholarly journals Study of proteolytic activity in rats kidney and liver during the development of chronic alcoholic intoxication

2021 ◽  
Vol 85 (2) ◽  
pp. 42-46
Author(s):  
A. Serbin ◽  
Y. Komar ◽  
T. Koval ◽  
O. Kharchenko ◽  
T. Andriychuk
Keyword(s):  
Proteolytic Activity ◽  
Alcohol Intoxication ◽  
Pathological Condition ◽  
Diagnostic Methods ◽  
Male Rats ◽  
Ethanol Administration ◽  
Intragastric Administration ◽  
Serine Proteinases ◽  
Chronic Alcohol ◽  
Chronic Alcohol Intoxication

The study of biochemical processes in chronic alcohol intoxication is a topical issue of our time. A deeper understanding of the mechanism of action of proteinases in this pathological condition will help in the development and search for non-invasive diagnostic methods, thereby minimizing the risk of harming human health during complex diagnostic procedures. In our experiment, we investigated the general proteolytic activity, the activity of metalloproteinases and serine proteinases in the liver and kidneys of rats on an experimental model of chronic alcohol intoxication on days 1, 3, 7, and 11 of the experiment, as well as on days 21 and 28 after the cessation of ethanol administration. Male rats weighing 180–200 g were modeled for chronic alcohol intoxication by intragastric administration of 30% ethyl alcohol solution for 10 days on an empty stomach, at the rate of 2 ml per 100 g of animal weight. Liver and kidney homogenate by well-known methods. The concentration was determined by the Bradford method. The total proteolytic activity, the activity of metalloproteinases and serine proteinases were determined by the caseinolytic method with modifications. The total proteolytic activity, the activity of metalloproteinases and serine proteinases were determined by the caseinolytic method with modifications. It was shown that on the 3rd, 7th and 11th days of the experiment in the liver there was an increase in the total proteolytic activity and the activity of metalloproteinases. The activity of serine proteinases significantly increased on days 3 and 7 of the study. In the kidneys, a significant increase in all studied activities was observed only on the 3rd day. Such differences in the activities of metalloproteinases and serine proteinases can be associated with the different roles of these enzymes in physiological processes. Thus, we observed an increase in the activity of serine proteinases in acute intoxication, and in metalloproteinases in chronic intoxication.

scholarly journals Proteolytic activity of rat blood plasma and liver under chronic alcohol intoxication

2020 ◽  
Vol 81 (2) ◽  
pp. 18-21
Author(s):  
A. Serbin ◽  
T. Koval ◽  
O. Kharchenko
Keyword(s):  
Alcohol Abuse ◽  
Blood Plasma ◽  
Proteolytic Activity ◽  
Alcohol Intoxication ◽  
Male Rats ◽  
World Health ◽  
Ethanol Administration ◽  
Chronic Alcohol ◽  
Chronic Alcohol Intoxication ◽  
The World

Today, the problem of alcohol abuse is quite relevant around the world. According to the World Health Organization, alcohol abuse is one of the three causes of premature death and is one of the main etiological factors that that cause the development of chronic diseases of the liver and other organs. The severity of clinical manifestations and the prognosis of further dynamics of ongoing alcohol intoxication can be investigated by studying the composition of proteins in tissues, as it is known that this pathology has a pronounced protein dystrophy and active proteolysis. Although much is known today about the effects of alcohol on proteolytic systems, many questions still need to be explored, as proteomic studies can be used to find biomarkers and therapeutic targets for ethanol abuse. The aim of the study was: to investigate the proteolytic activity of blood plasma and liver of rats in chronic alcohol intoxication at 11 and 21 days of the experiment. The model of chronic alcohol intoxication was modeled on male rats – weight 180-200g by intragastric administration of 30% ethyl alcohol solution for 10 days on an empty stomach, at the rate of 2 ml per 100 g of animal weight. Total proteolytic activity, activity of metalloproteinases and serine proteinases were determined by the method of determining caseinolytic activity. The protein concentration was determined by the Bradford method. Statistical processing of the study results was performed by conventional methods of variation statistics. It was found that on the 11th day from the beginning of ethanol administration to rats, there was an increase in total proteolytic activity and activity of metetalloproteinases in blood plasma and liver.

Behavior and the catecholamine system of the brain in two generations of the descendants of male rats with chronic alcohol intoxication

2013 ◽  
Vol 7 (2) ◽  
pp. 135-138 ◽  
Author(s):  
A. G. Veretinskaya ◽  
N. L. Vekshina ◽  
A. V. Stanishevskaya ◽  
I. Yu. Shamakina ◽  
V. A. Tomilin ◽  
...  
Keyword(s):  
Alcohol Intoxication ◽  
Male Rats ◽  
Chronic Alcohol ◽  
Chronic Alcohol Intoxication ◽  
Two Generations ◽  
The Brain

scholarly journals Impact of Craniocerebral Hypothermia and Cryopreserved Cord Blood on Reproductive Function of Male Rats with Chronic Alcohol Intoxication

2020 ◽  
Vol 30 (4) ◽  
pp. 369-379
Author(s):  
Іvan Lomakin ◽  
◽  
Olga Kudokotseva ◽  
Vladyslav Babiichuk ◽  
Yevhenii Kryshtal ◽  
...  
Keyword(s):  
Cord Blood ◽  
Testosterone Level ◽  
Alcohol Intoxication ◽  
Reproductive Function ◽  
Male Rats ◽  
Chronic Alcohol ◽  
Chronic Alcohol Intoxication ◽  
Sexual Activation ◽  
The Impact ◽  
Testosterone Synthesis

In this work, we have studied the impact of chronic alcohol intoxication (CAI) on reproductive function in male rats. Prolonged alcohol abuse has been shown to significantly reduce the manifestation rate of sexual activation and testosterone level in blood serum of male rats with CAI following exposure to a receptive female, as well as to change their seminal fl uid composition. The rhythmic craniocerebral hypothermia (rCCH), combined with the administration of cryopreserved cord blood leukoconcentrate (cCBL) ensured to a greater extent the dynamics of functional integrity recovery of the CAI-altered reproductive system in male rats, if compared to each of these methods used solely. The authors have hypothesized the rCCH and cCBL to activate the hypothalamicpituitary system, affecting thereby the testosterone synthesis and spermatogenesis stimulation.

scholarly journals Study of the absorptive intestinal function in an experimental model of chronic alcohol intoxication

2012 ◽  
Vol 93 (3) ◽  
pp. 499-502 ◽  
Author(s):  
L M Yakovleva ◽  
S V Lezhenina ◽  
Zh V Maslova ◽  
S V Kupriyanov
Keyword(s):  
Alcohol Intoxication ◽  
White Male ◽  
Ethanol Solution ◽  
Male Rats ◽  
Muscle Membrane ◽  
Epithelial Layer ◽  
Chronic Alcohol ◽  
Daily Consumption ◽  
Chronic Alcohol Intoxication ◽  
Rate Of Absorption

Aim. To study the absorptive function of the rat intestine on the background of prolonged alcohol intoxication. Methods. The study was performed on 24 nonlinear white male rats (12 animals in the control and experimental groups) weighing 220-240 g, which had a daily consumption of 20° ethanol solution (ethyl alcohol) for the duration of 180 days. After 6 months perfusion of the isolated segment of jejunum with a dextrose (glucose) solution 35 mmol/L was performed by in the rats the method A.M. Ugolev. The luminescence-histochemical method was used to examine the content of catecholamines and serotonin in the neuroamine containing structures. Results. In the control rats, the rate of absorption in the first 10 min of perfusion reached a maximum value with a further decrease in the intensity of absorption. The content of serotonin and catecholamines in the absorptive epithelial layer of the jejunal villi was lower than in the muscle membrane. In the experimental rats the content of biogenic amines in the wall of the jejunum increased: the amount of serotonin in the absorptive layer of the villi increased up to 122% and in the muscle membrane - up to 126%. The content of catecholamines also increased up to 154% in the muscle membrane and up to 124% in the epithelial layer in the area of the villi compared with controls. In conditions of chronic alcohol intoxication the rate of absorption of dextrose (glucose) during perfusion in the first 30 minutes was reduced by 33% compared with the control. During the next hour, the intensity of absorption reduced by 45% (55% of control). After 1.5 hours the absorption increased slightly, accounting for 64% of the control value and by the end of the 2nd hour the intensity of the absorption reached 91%. Conclusion. An increase in content of catecholamines and serotonin in the mucous and muscular layers of the intestine in chronic alcohol intake and a decrease in the intensity of the absorption of dextrose (glucose) was established; in the setting if modified combined effects of neurotransmitters on smooth muscle cells an increased motor effect is formed, which is accompanied by rapid evacuation of the intestinal chyme with a reduction in the rate of absorption

scholarly journals Pharmacological correction of metabolic disorders in experimental acute pancreatitis on the background of chronic alcohol intoxication

2018 ◽  
Vol 4 (4) ◽  
pp. 9-19
Author(s):  
Olga Bushmina ◽  
Svetlana Dolgareva ◽  
Alexander Konoplya ◽  
Aleksey Loktionov
Keyword(s):  
Acute Pancreatitis ◽  
Metabolic Disorders ◽  
Healthy Adult ◽  
Alcohol Intoxication ◽  
Ethanol Solution ◽  
Intragastric Administration ◽  
Chronic Alcohol ◽  
Ethanol Intoxication ◽  
Experimental Acute Pancreatitis ◽  
Chronic Alcohol Intoxication

Introduction. Acute pancreatitis and pre-existing diseases (chronic alcohol intoxication) are challenging issues of modern surgery in terms of frequency of deaths and the number of complications. Objective. To identify the best combination of immunomodulators, antioxidants and hepatoprotectors to correct immunometabolic disorders in acute destructive pancreatitis on the background of chronic alcohol intoxication (CAI). Materials and methods. Studies were conducted on 377 healthy adult Wistar rats weighing 150-200 g. Alcohol intoxication was modeled by forced intragastric administration of a 20% ethanol solution at a dose of 3 ml/kg or 2.92 g/kg at 24 hours for 5 days, 30 days (CAI-30) or 60 days (CAI-60). Acute destructive pancreatitis (ADP) was modeled after R.N. Wang et al. (1995), modified by S.A. Alekhin et al. (2006). The efficacy of combining Hepon (5 mg/kg, orally, at 24 hours, No. 14), Hypoxen (750 mg/kg, orally, at 24 hours, No. 14), Phosphogliv (800 mg/kg, orally, at 24 hours, No. 14), Glutoxim (20 mg/kg, intramuscularly, at 24 hours, No. 5), Mexidol (50 mg/kg, intraperitoneally, at 24 hours, No. 14) and Heptral (760 mg/kg, intraperitoneally, at 24 hours , No. 5) was examined in animals with ADP on the background of CAI-30 and CAI-60. Results and discussion. Different degrees of ethanol intoxication depending on time leads to the development of an impaired capacity of hepatocytes, as well as immune and metabolic disorders. The administration of Phosphogliv, Hypoxen and Hepon in case of CAI-30+ADP normalizes 12.1% and corrects 60.6% of the changed values. The combination of Heptral, Glutoxim and Mexidol normalizes 57.8% and corrects 42.4% of the values. In case of CAI-60+ADP, Phosphogliv, Hypoxen and Hepon normalizes 11.8% and corrects 38.2% of the values. The combination of Heptral, Glutoxim and Mexidol normalizes 17.6% and corrects 67.6% of the values, respectively. Conclusion. In case of ADP along with CAI-30 and CAI-60, the combination of Heptral, Glutoxim and Mexidol is more preferable than that of Phosphogliv, Hypoxen and Hepon.

Nitrosorbidi Stress and Apoptosis of Neurons in the CA1 Zone of the Hippocampus in the Conditions of Modeling Chronic Alcohol Intoxication: Neuroprotective Effects of Tietema

10.12737/5906 ◽  
2014 ◽  
Vol 21 (3) ◽  
pp. 85-91
Author(s):  
Кучер ◽  
T. Kucher ◽  
Кучеренко ◽  
L. Kucherenko ◽  
Моргунцова ◽  
...  
Keyword(s):  
Protective Effect ◽  
Alcohol Intoxication ◽  
Ethanol Solution ◽  
Ethanol Administration ◽  
Control Group ◽  
Chronic Alcohol ◽  
Experimental Animals ◽  
Chronic Alcohol Intoxication ◽  
Chronic Alcoholization ◽  
Hippocampal Ca1

Fulfilled researches showed that modeling of chronic alcohol intoxication by daily intragastric ethanol administration to non-pedigreed male white rats (first 10 days – 15% ethanol solution in dose 4 g/kg, next 10 days – 15% ethanol solution in dose 6 g/kg, then 10 days – 25% ethanol solution in dose 4 g/kg) leads to activation of nitrosorbidi stress reactions and initiation of neuro-apoptosis. Thus, as compared with healthy animals group, in rats’ brain after chronic ethanol administration it was revealed significant increase of nitro-tyrosine (nitrosorbidi stress marker) in cytosolic and mitochondrial fractions of brain homogenate against the background of revealing of apoptosis molecular markers in hippocampal CA1 zone neurons (fragmentation of neuron´s nuclei of hippocampal CA1 zone, increase of density and ratio of apoptotic- and destructive-changed cells) as well as deprivation of anti-apoptotic mechanisms (decrease of density of Bcl-2-positive neurons in hippocampal CA1 zone and decrease of Bcl-2 protein concentration in hippocampus). Subsequent 14-day intragastric administration of complex neuro-metabolic cerebro-protector Thiocetam in dose 100 mg/kg to the animals after chronic alcoholization showed significant protective effect on hippocampal CA1 zone neurons. Thus, Thiocetam administration resulted in decrease of nitrotyrosine levels in mitochondrion and in cytosol of brain of animals with chronic alcoholization as compared with untreated animals and with animals receiving Piracetam. Thiocetam administration to experimental animals led to significant decrease of density of apoptotic- and destructive-changed neurons of hippocampal CA1 zone and ratio of apoptotic-changed cells as compared with control group and animals receiving Piracetam. Course of treatment with Thiocetam increases significantly the density of Bcl-2-positive neurons in CA1 zone of hippocampus and significantly increases concentration of Bcl-2 in brain tissue of experimental animals as compared with control group and animals which received Piracetam according the same schedule. The increase of Bcl-2 protein expression in animals received Thiocetam testifies to the activation of anti-apoptotic defense of damaged neurons under the influence of this preparation. We suppose that one of the molecular mechanisms of neuro-protective effect of Thiocetam in chronic intoxication is the breaking of NO-depended mechanisms of neuro-apoptosis.

scholarly journals Influence of chronic ethanol intake on obesity, liver steatosis and hyperlipidaemia in the Zucker fa/fa rat

1985 ◽  
Vol 54 (1) ◽  
pp. 5-13 ◽  
Author(s):  
C. Karsenty ◽  
F. Chanussot ◽  
M. Ulmer ◽  
G. Debry
Keyword(s):  
Wistar Rats ◽  
Alcohol Intoxication ◽  
Fatty Infiltration ◽  
Liver Steatosis ◽  
Male Rats ◽  
Zucker Rats ◽  
Chronic Alcohol ◽  
Metabolic Disturbances ◽  
Chronic Alcohol Intoxication ◽  
Hepatic Triacylglycerol

1. The effect of chronic alcohol intoxication on metabolic disturbances and fatty infiltration and degeneration was studied in genetically obese, hyperlipoproteinaemic, fa/fa Zucker rats.2. Sixteen obese Zucker (fa/fa) rats, sixteen lean Zucker rats (Fa/-)and sixteen Wistar rats, all male rats aged 7–8 weeks, were given either a control (C)diet (13% of energy from protein, 37% from fat, 50% from carbohydrate) or an ethanol (E) diet (13% of energy from protein, 37% from fat, 14% from carbohydrate, 36% from ethanol) for 4 weeks.3. The fa/fa rats given diet E consumed more energy than those given diet C, but after 4 weeks the weight gains and degrees of obesity were similar for both groups. With both diets, the developed hyperlipidaemia could be explained by the hyperinsulinaemia. Both hypertriglyceridaemia and hypercholesterolaemia were lower in fa/fa rats eating diet E than in those given diet C. Fatty infiltration of the liver, as assessed by hepatic triacylglycerol and cholesterol contents, was observed with both diets, but for fa/fa rats it was less extreme in those given diet E.

Chronic alcohol intoxication is not accompanied by an increase in calpain proteolytic activity in cardiac muscle of rats

2017 ◽  
Vol 82 (2) ◽  
pp. 168-175 ◽  
Author(s):  
Yu. V. Gritsyna ◽  
N. N. Salmov ◽  
A. G. Bobylev ◽  
I. S. Fadeeva ◽  
N. I. Fesenko ◽  
...  
Keyword(s):  
Cardiac Muscle ◽  
Proteolytic Activity ◽  
Alcohol Intoxication ◽  
Chronic Alcohol ◽  
Chronic Alcohol Intoxication

scholarly journals Changes in the dopaminergic system in the hypothalamus and midbrain of rats at chronic complex alcohol-morphine intoxication

2021 ◽  
Vol 18 (2) ◽  
pp. 212-219
Author(s):  
I. M. Vialichko ◽  
S. V. Lelevich ◽  
V. V. Lelevich
Keyword(s):  
Dopaminergic System ◽  
Alcohol Intoxication ◽  
Hplc Method ◽  
Male Rats ◽  
Dopamine Turnover ◽  
Chronic Alcohol ◽  
Dopamine System ◽  
Chronic Alcohol Intoxication ◽  
The Brain

Recently, there have been more and more indications of alcoholic pathology burdened by drug use and vice versa. It is evident that the dopamine system plays an important role in the development of addiction when using drugs and alcohol. Experimentally, the long-term combined effect of psychoactive substances on neuromendatory changes in the dopaminergic system in the brain is poorly understood and requires more detailed consideration.The aim of the study was to examine the changes in the dopaminergic system in the hypothalamus and midbrain of rats during chronic alcohol intoxication, as well as a complex administration of morphine and ethanol.The experiments were carried out on white outbred male rats. Using the HPLC method, the levels of dopamine and its metabolites were determined in the regions of the brain during chronic alcohol intoxication, as well as with a combined administration of morphine and ethanol with various durations (7, 14 and 21 days).Chronic alcohol intoxication led to the signs of acceleration of the dopamine turnover only in the hypothalamus on the 7th and 14th days and its accumulation during three weeks of alcoholization. In the midbrain, a decrease in the concentration of the neurotransmitter was revealed when ethanol was injected for 7 days. Co-administration of surfactants was accompanied by slightly different changes: complex 7- and 21-day alcohol-morphine intoxication is accompanied by the dopamine accumulation in the hypothalamus and the decrease in the neurotransmitter turnover in the midbrain on the 14th and 21st days.

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