scholarly journals Characterization of human oocyte development and the role of mitochondrial activity in in vitro germ cell differentiation

2019 ◽  
Author(s):  
Alisha M. Bothun
Keyword(s):  
Cell Differentiation ◽  
Germ Cell ◽  
Oocyte Development ◽  
Human Oocyte ◽  
Mitochondrial Activity ◽  
Germ Cell Differentiation

Role of Mael in early oogenesis and during germ-cell differentiation from embryonic stem cells in mice in vitro

Zygote ◽  
2013 ◽  
Vol 22 (4) ◽  
pp. 513-520 ◽  
Author(s):  
I. Bahena ◽  
E. Xu ◽  
M. Betancourt ◽  
E. Casas ◽  
Y. Ducolomb ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Differentiation ◽  
Embryonic Stem Cells ◽  
Germ Cell ◽  
Embryonic Stem ◽  
Oocyte Growth ◽  
Germ Cell Differentiation ◽  
Rnai Technology

SummaryIn a previous study, we have identified a set of conserved spermatogenic genes whose expression is restricted to testis and ovary and that are developmentally regulated. One of these genes, the transcription factor Mael, has been reported to play an essential role in mouse spermatogenesis. Nevertheless, the role of Mael in mouse oogenesis has not been defined. In order to analyse the role of Mael in mouse oogenesis, the expression of this gene was blocked during early oogenesis in mouse in vitro using RNAi technology. In addition, the role of Mael during differentiation of embryonic stem cells (ESC) into germ cells in vitro was analysed. Results show that downregulation of Mael by a specific short interfering RNA disrupted fetal oocyte growth and differentiation in fetal ovary explants in culture and the expression of several germ-cell markers in ESC during their differentiation. These results suggest that there is an important role for Mael in early oogenesis and during germ-cell differentiation from embryonic stem cells in mouse in vitro.

scholarly journals Critical role of Emx2 in the pluripotency – differentiation transition in male gonocytes via regulation of FGF9/NODAL pathway

Reproduction ◽  
2016 ◽  
Vol 151 (6) ◽  
pp. 673-681 ◽  
Author(s):  
Ma Tian-Zhong ◽  
Chen Bi ◽  
Zhang Ying ◽  
Jing Xia ◽  
Peng Cai-Ling ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Mouse Model ◽  
Germ Cell ◽  
Germ Cells ◽  
Critical Role ◽  
Genital Ridge ◽  
Germ Cell Differentiation ◽  
Pluripotency Markers

Abstract Emx2 deletion impairs the growth and maintenance of the genital ridge. However, its role in subsequent germ cell differentiation during embryonic stages is unknown. Using a tamoxifen-inducible Cre-loxP mouse model (Emx2flox/flox, Cre-ERTM, hereafter called as Emx2 knockdown), we showed that germ cell differentiation was impaired in Emx2-knockdown testes. Representative characteristics of male germ cell differentiation, including a reduced ability to form embryonic germ (EG) cell colonies in vitro, down-regulation of pluripotency markers and G1/G0 arrest, did not occur in Emx2-knockdown testes. Furthermore, FGF9 and NODAL signalling occurred at abnormally high levels in Emx2-knockdown testes. Both blocking FGF9 signalling with SU5402 and inhibiting NODAL signalling with SB431542 allowed germ cells from Emx2-knockdown testes to differentiate in vitro. Therefore, EMX2 in somatic cells is required to trigger germ cell differentiation in XY foetuses, posterior to its previously reported role in the growth and maintenance of the genital ridge.

Role of Sex Chromosomes in the Control of Male Germ-Cell Differentiation

1991 ◽  
Vol 637 (1 The Male Germ) ◽  
pp. 64-73 ◽  
Author(s):  
MARY HANDEL ◽  
PATRICIA A. HUNT ◽  
MARY C. KOT ◽  
CYNTHIA PARK ◽  
MARK SHANNON
Keyword(s):  
Cell Differentiation ◽  
Germ Cell ◽  
Sex Chromosomes ◽  
Male Germ Cell ◽  
Germ Cell Differentiation

Role of Beta-Catenin in Post-Meiotic Male Germ Cell Differentiation.

2011 ◽  
Vol 85 (Suppl_1) ◽  
pp. 563-563
Author(s):  
Manjeet K. Rao ◽  
Yao-Fu Chang ◽  
Jennifer Lee ◽  
Amiya P. Sinha-Hikim
Keyword(s):  
Cell Differentiation ◽  
Germ Cell ◽  
Male Germ Cell ◽  
Beta Catenin ◽  
Germ Cell Differentiation

scholarly journals In Vitro Germ Cell Differentiation from Cynomolgus Monkey Embryonic Stem Cells

PLoS ONE ◽  
2009 ◽  
Vol 4 (4) ◽  
pp. e5338 ◽  
Author(s):  
Kaori Yamauchi ◽  
Kouichi Hasegawa ◽  
Shinichiro Chuma ◽  
Norio Nakatsuji ◽  
Hirofumi Suemori
Keyword(s):  
Stem Cells ◽  
Cell Differentiation ◽  
Embryonic Stem Cells ◽  
Germ Cell ◽  
Cynomolgus Monkey ◽  
Embryonic Stem ◽  
Germ Cell Differentiation

A possible role of Reproductive homeobox 6 in primordial germ cell differentiation

2011 ◽  
Vol 55 (10-11-12) ◽  
pp. 909-916 ◽  
Author(s):  
Chang Liu ◽  
Paichi Tsai ◽  
Ana-Marie García ◽  
Brandon Logeman ◽  
Tetsuya S. Tanaka
Keyword(s):  
Cell Differentiation ◽  
Germ Cell ◽  
Primordial Germ Cell ◽  
Germ Cell Differentiation
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