Análise da terapia a laser de baixa intensidade em culturas in vitro de bactérias e fungo

Introduction: Low intensity laser therapy has been used to accelerate the ulcers healing due to its healing, anti-inflammatory, analgesic and anti-edematous effects. Contaminations by bacteria or fungi on these ulcers commonly happen, constituting a major complication of healing, but there is no consensus about laser therapy bactericidal or fungicidal effect. Objective: To analyze the effects of different times of appliantion using the 808nm and 660nm lasers on cultures of different bacterial species and fungi in vitro. Method: Fifteen Petri plates were prepared, 12 plates containing Gram-positive (3 Staphylococcus aureus plates, 3 Enterococcus plates) and gram negative bacterias (3 Escherichia coli, 3 Klebsiella pneumonia plates) and 3 Petri plates with the fungi Candida albicans. Plates were randomly divided into 3 groups with 5 plates each group. Plates of Group 1 were irradiated with 660 nm laser at nine different points (A, A1, A2/B, B1, B2/C, C1, C2), and points A/B/C reciebed only one application, points A1,B1,C1 received 2 applications and points A2,B2,C2 received 3 applications. Plates from group 2 where irradiated with 660nm also in nine diferente points, following the same criterea of group 1, while plate of group 3 where used as control, without irradiation. Groups 1 and 2 were irradiated with 144 J/cm² dose at point A (irradiation time of 2 minutes and 15 seconds), point B (irradiation time of 1 minute 7 seconds) and at point C (40 seconds). After the irradiations the plates were incubated for 24 hours. The cultures were visually examined to check the presence or not of the inhibition zone. Results: In all the groups, no zone of inhibition nor growth were observed, indicating no bactericidal/fungicidal and/or bactericidal effect, in other words, in all groups and locations with different time exposures (A - 30 mW, B - and C 60 mW - 100 mW) the growth of bacteria and fungi were equal. Conclusion: Low intensity laser therapy (808nm and 660nm) did not produce bactericidal and/or bacteriostatic effects, and even no bio-stimulant effect on the bacteria.

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Correction of the body state with low-intensity laser irradiation in modeling adrenal toxemia in rats

ZHurnal «Patologicheskaia fiziologiia i eksperimental`naia terapiia» ◽

10.25557/0031-2991.2019.04.81-87 ◽

2019 ◽

pp. 81-87

Author(s):

А.В. Дерюгина ◽

Н.В. Жемарина ◽

М.Н. Иващенко ◽

А.Г. Самоделкин ◽

П.С. Игнатьев ◽

...

Keyword(s):

Laser Therapy ◽

Electrophoretic Mobility ◽

Adrenal Glands ◽

The Body ◽

Prolonged Release ◽

Low Intensity ◽

Group 2 ◽

Low Intensity Laser Therapy ◽

Intensity Laser ◽

Group 1

Срыв адаптационных возможностей организма может стать патогенетической основой различных заболеваний. Длительный выброс адреналина в кровь вызывает кататоксические эффекты, что делает актуальным поиск эффективных средств и методов коррекции гиперадреналинемии. Цель работы - изучение влияния низкоинтенсивного лазерного излучения (НИЛИ) на электрофоретическую подвижность эритроцитов, процессы липопероксидации и структуру надпочечников при адреналовой токсемии у крыс. Методика. Адреналовую токсемию моделировали внутрибрюшинным введением адреналина-гидрохлорида (0,1 мг/кг). Животным 1-й группы вводили только адреналин, животных 2-й - через 30 мин после введения адреналина облучали НИЛИ, крысы 3-й группы получали только воздействие НИЛИ, контролем служили интактные животные (4-я группа). Воздействие НИЛИ проводилось на затылочную область в течение 10 мин. В качестве источника излучения применяли аппарат лазерный терапевтический «Успех» (ГП «Восход»), основанный на низкоинтенсивном импульсном излучении (длина волны - 0,89±0,05 мкм, частота импульсов 415 Гц, плотность средней мощности излучения - 193 мкВт/см2, площадь облучаемой поверхности 20 см2). Забор крови производили через 1 ч, 24 ч и 1 нед после начала эксперимента. Определяли уровень малонового диальдегида по реакции с тиабарбитуровой кислотой; электрофоретическую подвижность эритроцитов изучали методом микроэлектрофореза. Структуру надпочечников изучали морфологическими методами. Результаты. Показано, что НИЛИ на фоне адреналина (2-я группа) через 1 нед нивелировало снижение электрофоретической подвижности эритроцитов, восстанавливало уровень малонового диальдегида до значений интактной группы. Морфологическая картина надпочечников в этой группе не отличалась от таковой у интактных животных, тогда как при действии адреналина (1-я группа) не происходило восстановления исследуемых показателей до физиологической нормы. Заключение. Полученные в ходе эксперимента данные свидетельствуют о корригирующем действии низкоинтенсивного лазерного излучения при адреналовой токсемии у животных. Механизм действия низкоинтенсивного лазерного излучения, вероятно, носит системный характер и связан с воздействием на стресс-реализующие системы организма. Maladaptation results in the third stage of stress, exhaustion, and, thus, may underlie many diseases. Prolonged release of epinephrine into blood exerts catatoxic effects; therefore, searching for effective methods for correction of hyperadrenalinemia is relevant. Presently, low-intensity laser therapy (LILT) is commonly used in medicine. However, the introduction of LILT to clinical practice is largely empirical. The aim of this study was to analyze the effect of LILT on electrokinetic potential of red blood cells (RBC), lipid peroxidation (LPO), and the morphology of adrenal glands in adrenal toxemia in rats. Methods. Adrenal toxemia was modeled by intraperitoneal administration of epinephrine hydrochloride (0.1 mg/kg) to rats. Animals of group 1 received epinephrine alone; animals of group 2 received LILT 30 min prior to epinephrine administration; rats of group 3 received LILT alone; and intact rats of group 4 were used as control. Blood was withdrawn at one hour, 24 h, and one week of the experiment onset. The RBC zeta potential, which characterizes the morpho-functional state of membranes, was determined by measuring the effect of low-intensity laser therapy on RBC electrophoretic mobility using microelectrophoresis. LPO as an indicator of cell membrane injury was determined by concentration of malonic dialdehyde (MDA). The structure of adrenal glands was studied histologically. Results. LILT combined with epinephrine (group 2) alleviated the decrease in RBC electrophoretic mobility and restored the MDA level to values of the intact group at one week. Morphological structure of adrenal glands in group 2 did not differ from that in control whereas epinephrine (group 1) did not restore the study parameters to physiologically normal values. Conclusion. The study showed that LILT exerted a correcting effect in experimental adrenal toxemia. The mechanism of this effect is likely systemic and related with the effect on stress systems of the body.

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Effects of low intensity laser in in vitro bacterial culture and in vivo infected wounds

Revista do Colégio Brasileiro de Cirurgiões ◽

10.1590/s0100-69912014000100010 ◽

2014 ◽

Vol 41 (1) ◽

pp. 49-55 ◽

Cited By ~ 14

Author(s):

Pâmela Rosa Pereira ◽

Josue Bruginski De Paula ◽

Juliette Cielinski ◽

Marcelo Pilonetto ◽

Luiz Carlos Von Bahten

Keyword(s):

Laser Therapy ◽

Control Group ◽

Type I ◽

Low Intensity ◽

Infected Wounds ◽

Male Wistar Rats ◽

Low Intensity Laser Therapy ◽

Intensity Laser

OBJECTIVE: to compare the effects of low intensity laser therapy on in vitro bacterial growth and in vivo in infected wounds, and to analyze the effectiveness of the AsGa Laser technology in in vivo wound infections. METHODS: in vitro: Staphylococcus aureus were incubated on blood agar plates, half of them being irradiated with 904 nm wavelength laser and dose of 3J/cm2 daily for seven days. In vivo: 32 male Wistar rats were divided into control group (uninfected) and Experimental Group (Infected). Half of the animals had their wounds irradiated. RESULTS: in vitro: there was no statistically significant variation between the experimental groups as for the source plates and the derived ones (p>0.05). In vivo: there was a significant increase in the deposition of type I and III collagen in the wounds of the infected and irradiated animals when assessed on the fourth day of the experiment (p=0.034). CONCLUSION: low-intensity Laser Therapy applied with a wavelength of 904nm and dose 3J/cm2 did not alter the in vitro growth of S. aureus in experimental groups; in vivo, however, it showed significant increase in the deposition of type I and III collagen in the wound of infected and irradiated animals on the fourth day of the experiment.

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Antimicrobial Photodinamic Therapy Combined with Laser Photobiomodulation in the Treatment of Skin Wounds in a Dog (Canis lupus familiaris)

Acta Scientiae Veterinariae ◽

10.22456/1679-9216.116169 ◽

2021 ◽

Vol 49 ◽

Author(s):

Fernando Alzamora Filho ◽

Manoel Luiz Ferreira ◽

Marcus Vinícius Alves Da Silva ◽

Anna Claudia Mombrini Silva Barbosa ◽

Bruna Guedes Carvalho ◽

...

Keyword(s):

Methylene Blue ◽

Laser Therapy ◽

Canis Lupus ◽

Conventional Therapy ◽

Irradiation Time ◽

Canis Lupus Familiaris ◽

Low Intensity ◽

Low Intensity Laser Therapy ◽

Intensity Laser ◽

Wounded Area

Background: Laser photobiomodulation has been used in the treatment of various injuries and diseases. This promotes modulation of the inflammatory process, edema reduction and devitalized tissue regeneration. The advantages of Antimicrobial photodynamic therapy are its easy application and the absence of side effects. Other advantages are the cost of the therapy, minimal damage to animal tissue, the broad spectrum of action, and efficiency against strains resistant to antimicrobials. The aim of this study was to report the clinical and their resolution in a female dog with a traumatic, infected wound treated with laser phototherapy as an alternative therapy method.Case: A 3-year-old bitch Border Collie, weighing 18 kg, from the municipality of Ilhéus, Bahia, Brazil, waspresented for examination with a history of traumatic laceration of the left thoracic member. On the anamnesis, it was reported that the patient presented with laceration of the left thoracic member. The wound was cleaned and an antibiotic [30 mg/kg of 12/12 h] and anti-inflammatory [0.1 mg/kg every 24 h were prescribed, both for 5 days]. Twenty-four h after the surgical procedure, there was dehiscence of the sutures, with daily topical therapy based on zinc oxide for secondary intention healing. Upon physical examination, the wound was found to be contaminated with swollen and erythematous edges, an ulcerated area with devitalized tissue, serous exudate, and 8.8 cm2 of wounded area. Given the macroscopic characteristics of the lesion, phototherapy was associated with conventional therapy until complete healing of the wound, with three weekly applications at 48 h intervals. Initially, the wound was cleaned with saline solution at 0.9% and a single treatment with aPDT was scheduled due to the high degree of contamination. The dosimetry parameters of irradiation were calculated according to the wounded area with a diode laser of 0.1W of power, continuous emission, spot area of 0.028 cm2, and energy of 9 J per application point. A gauze imbibed with 1 mL of methylene blue aqueous solution (300 μM), which was the photosensitizer was applied to the lesion, with a pre-irradiation time of 5 min, after which it was irradiated with red laser (RL) (λ = 660nm) for 90 s per point, using the sweeping technique. The edge of the lesion was irradiated with infrared laser (IRL) (λ = 808 nm), total energy of 5 J, using the technique of specific points and 1 J of energy/point. After aPDT, low-intensity laser therapy (LILT) sessions were set up with RL and IRL, with energies of 0.5 J/point and 1 J/point, respectively. The wound was cleaned daily, protected with a bandage, and clinically evaluated until complete regression.Discussion: In the present case, methylene blue was used as a photosensitizer, but it is noteworthy that, apparently, each microorganism responds differently to photosensitizers. Thus, the therapy becomes specific for each application, for example: the type of photosensitizer, its concentration, pre-irradiation time, type of light used in photosensitization, wavelength, energy, power, mode delivery of light. Thus, for the best result, the specific protocol in each application should be used Low-intensity laser therapy is an easily executed technique with effective results. The use of PDT associated with photobiomodulation therapy enabled rapid healing of the cutaneous wound, in addition to an improvement in clinical signs and pain caused by the lesion. The technique proved to be an efficient alternative in the treatment of wounds, whether used in isolation or associated with conventional therapy. Keywords: methylene blue, healing, wound, photobiomodulation, low-level laser.Título: Terapia fotodinâmica antimicrobiana combinada com a fotobiomodulação a laser no tratamento de ferida cutânea em cão (Canis lupus familiaris).

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"Low intensity laser therapy: possible strategy to restore fertility in premature ovarian failure induced by chemotherapy?"

10.26226/morressier.573c1514d462b80296c98bd9 ◽

2016 ◽

Author(s):

Ignacio de Zuñiga

Keyword(s):

Laser Therapy ◽

Premature Ovarian Failure ◽

Ovarian Failure ◽

Low Intensity ◽

Low Intensity Laser Therapy ◽

Intensity Laser

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Laser therapy of chronic inflammatory diseases of the uterine appendages

Journal of obstetrics and women s diseases ◽

10.17816/jowd88663 ◽

2004 ◽

Vol 53 (4) ◽

pp. 76-81

Author(s):

E. F. Condrina ◽

G. N. Ponomarenko

Keyword(s):

Laser Therapy ◽

Inflammatory Diseases ◽

Scientific Publications ◽

Chronic Inflammatory Diseases ◽

Advantages And Disadvantages ◽

Low Intensity ◽

The Past ◽

Low Intensity Laser Therapy ◽

Different Characteristics ◽

Intensity Laser

This article provides an overview of scientific publications by domestic and foreign authors over the past 10 years, devoted to the problem of using low-intensity laser therapy in the treatment of chronic inflammatory diseases of the uterine appendages; the analysis of indications and contraindications, advantages and disadvantages of using laser radiation with different characteristics in the treatment of chronic salpingo-oophoritis was carried out; the most significant aspects of the influence of laser therapy on the main pathogenetic mechanisms of the development of the inflammatory process of the uterine appendages are highlighted.

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