Evaluation of Residual Dentin after Conventional and Chemomechanical Caries Removal Using SEM

2007 ◽  
Vol 32 (2) ◽  
pp. 115-120 ◽  
Author(s):  
Fernanda Nahás Pires Corrêa ◽  
Leonardo Eloy Rodrigues Filho ◽  
Célia Regina Martins Delgado Rodrigues
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
In Vitro Study ◽  
Adhesive System ◽  
Smear Layer ◽  
Dentinal Tubules ◽  
Removal Method ◽  
Caries Removal ◽  
Scanning Electron

The purpose of this in vitro study was to analyze the residual dentinal surfaces following caries removal using rotatory instruments and two chemomechanical methods (Papacárie® and Carisolv®), by scanning electron microscopy (SEM). Thirty primary incisors were divided into three groups, according to the caries removal method used, and their residual dentin was examined under SEM (15). After caries removal, 15 of these teeth were restored with Single Bond (3M) adhesive system and Z100 Filtek composite resin (3M). The tags of the replicas were observed under SEM. The chemomechanical caries removal methods (Papacárie®and Carisolv®) formed an amorphous layer, similar to the smear layer and few exposed dentinal tubules;the conventional caries removal method produced a smooth and regular dentinal surface, with typical smear layer and exposed dentinal tubules. All groups showed abundant tag formation. Scanning electron microscopy analysis revealed a difference between dentin treated with rotatory instruments and that treated with chemomechanical methods in spite of the occurrence of a similar tag formation in both groups.

scholarly journals Remineralization Potential of Three Tooth Pastes on Enamel Caries

2017 ◽  
Vol 5 (5) ◽  
pp. 664-666 ◽  
Author(s):  
Rajnish K. Singhal ◽  
Balwant Rai
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
In Vitro Study ◽  
Fluoride Content ◽  
Significant Difference ◽  
Polarised Light ◽  
New Formulation ◽  
Scanning Electron

BACKGROUND: Different formulations of dentifrices exist in the market. Usually, single toothpaste is used by all family members including children. There is a big concern of fluoride ingestion with the toothpaste containing high fluoride content in children. Recently, new toothpaste (including toothpaste) with remineralization potential without fluoride content has been formulated.AIM: There is an urgent need to compare remineralization potential of this new formulation with the exiting dentifrices. Therefore, the present study has been undertaken to assess and compare the remineralization potential of three dentifrices with different compositions on artificially induced carious lesions in vitro by using scanning electron microscopy and polarised light microscopy.MATERIALS AND METHODS: The present in vitro study was conducted on 21 healthy extracted primary central incisor teeth surfaces, which were divided into three groups and were treated by three different dentifrices. Artificial demineralization was followed by remineralization using dentifrice slurry as per the group distribution. All the samples were studied for remineralization by using scanning electron microscopy and polarised light microscopy. Data were analysed using SPSS version 11 software.RESULTS: A significant difference was found between the remineralization potential of  incudent toothpaste and other toothpaste groups based on the analysis of polarised light microscopy and stereomicroscope. The remineralizing ability of  incudent toothpaste for artificial enamel lesions was found to be significantly higher than that of Colgate® and Crest toothpaste.CONCLUSIONS: The limitations of this study include, being a short term study, low sample size and in vitro experiment.  incudent toothpaste has exhibited a higher remineralizing potential as compared to fluoride based toothpaste in our study.

scholarly journals Particulated, Extracted Human Teeth Characterization by SEM–EDX Evaluation as a Biomaterial for Socket Preservation: An in vitro Study

Materials ◽  
2019 ◽  
Vol 12 (3) ◽  
pp. 380 ◽  
Author(s):  
José Luis Calvo-Guirado ◽  
Alvaro Ballester-Montilla ◽  
Piedad N De Aza ◽  
Manuel Fernández-Domínguez ◽  
Sergio Alexandre Gehrke ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Chemical Composition ◽  
In Vitro Study ◽  
Human Tooth ◽  
Human Teeth ◽  
Socket Preservation ◽  
Small Peak ◽  
Scanning Electron

The aim of the study was to evaluate the chemical composition of crushed, extracted human teeth and the quantity of biomaterial that can be obtained from this process. A total of 100 human teeth, extracted due to trauma, decay, or periodontal disease, were analyzed. After extraction, all the teeth were classified, measured, and weighed on a microscale. The human teeth were crushed immediately using the Smart Dentin Grinder machine (KometaBio Inc., Cresskill, NJ, USA), a device specially designed for this procedure. The human tooth particles obtained were of 300–1200 microns, obtained by sieving through a special sorting filter, which divided the material into two compartments. The crushed teeth were weighed on a microscale, and scanning electron microscopy (SEM) evaluation was performed. After processing, 0.25 gr of human teeth produced 1.0 cc of biomaterial. Significant differences in tooth weight were found between the first and second upper molars compared with the lower molars. The chemical composition of the particulate was clearly similar to natural bone. Scanning electron microscopy–energy dispersive X-ray (SEM–EDX) analysis of the tooth particles obtained mean results of Ca% 23.42 0.34 and P% 9.51 0.11. Pore size distribution curves expressed the interparticle pore range as one small peak at 0.0053 µm. This result is in accordance with helium gas pycnometer findings; the augmented porosity corresponded to interparticle spaces and only 2.533% corresponded to intraparticle porosity. Autogenous tooth particulate biomaterial made from human extracted teeth may be considered a potential material for bone regeneration due to its chemical composition and the quantity obtained. After grinding the teeth, the resulting material increases in quantity by up to three times its original volume, such that two extracted mandibular lateral incisors teeth will provide a sufficient amount of material to fill four empty mandibular alveoli. The tooth particles present intra and extra pores up to 44.48% after pycnometer evaluation in order to increase the blood supply and support slow resorption of the grafted material, which supports healing and replacement resorption to achieve lamellar bone. After SEM–EDX evaluation, it appears that calcium and phosphates are still present within the collagen components even after the particle cleaning procedures that are conducted before use.

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