scholarly journals EFFECT OF AMINO ACID ON THE DEVELOPMENT SKIN TISSUE ORGANOTYPIC CULTURE

2017 ◽  
Vol 19 (3) ◽  
pp. 71-76
Author(s):  
Natal'ya I Chalisova ◽  
Aleksander E Korovin
Keyword(s):  
Adverse Effect ◽  
Amino Acids ◽  
Tissue Culture ◽  
Cellular Proliferation ◽  
Organotypic Culture ◽  
Growth Zone ◽  
Rat Skin ◽  
Inhibiting Effect ◽  
Combined Action ◽  
Organotypic Tissue

The effect of the 20 coded amino acids was investigated on the development of the processes of the proliferation in the organotypic tissue culture of rat skin. The amino acids lysine, arginine and glutamine acid at 0,05 ng/ml concentration stimulated the cellular proliferation in the growth zone of explants by 29-30%. The mustard-like agent cyclophosphane at 1 mg/ml concentration inhibited the cellular proliferation. The delay of this inhibiting effect of cyclophosphane was observed by the combined action of stimulating amino acids with the cyclophosphane. Thus, the amino acids with the charge radicals can be protectors of the cellular proliferation by the toxic effect of the cyclophosphane on the skin. This effect can be used for the treatment of the mustard injury of skin and for the delay of the adverse effect of cytostatic in oncology (1 figure, 1 table, bibliography: 28 refs).

scholarly journals PROTECTIVE EFFECT OF AMINO ACIDS IN THE ORGANOTYPIC CULTURE OF THE ECTODERMAL GENESIS TISSUES AT THE CYCLOPHOSPHANE PRESENCE

2017 ◽  
Vol 19 (4) ◽  
pp. 47-52
Author(s):  
N I Chalisova ◽  
A E Korovin
Keyword(s):  
Adverse Effect ◽  
Amino Acids ◽  
Brain Cortex ◽  
Cellular Proliferation ◽  
Organotypic Culture ◽  
Growth Zone ◽  
The Other ◽  
Rat Skin ◽  
Inhibiting Effect ◽  
Organotypic Tissue

The effect of the 20 coded amino acids was investigated on the development of the processes of the proliferation in the organotypic tissue culture of rat skin and brain cortex. Some amino acids at 0.05 ng/ml concentration stimulated the cellular proliferation in the growth zone of explants. The other inhibited it. The combination of the stimulating and inhibiting amino acids - Leucine with Proline or Tyrosine-, lead to the proliferation stimulation by 24-32%. The mustard-like agent cyclophosphane at 1 mg/ml concentration inhibited the cellular proliferation. However, the delay of this inhibiting effect of cyclophosphane was observed by the using of combination of amino acids with cyclophosphane. Thus, the amino acids can be protectors of the cellular proliferation by the toxic effect of the cyclophosphane on the tissues of the ectodermal genesis. This effect can be used for the treatment of the mustard injury of skin, brain cortex and for the delay of the adverse effect of cytostatic in oncology

Protective influence of coded amino acids on the development of liver tissue culture in the presence of cytostatic

2020 ◽  
pp. 48-53
Author(s):  
N. I. Chalisova ◽  
V. K. Kozlov ◽  
A. B. Mulik ◽  
E. P. Zatsepin ◽  
T. A. Kostrova
Keyword(s):  
Amino Acids ◽  
Tissue Culture ◽  
Side Effects ◽  
Liver Tissue ◽  
Organotypic Culture ◽  
Growth Zone ◽  
Inhibitory Effect ◽  
Cytostatic Drugs ◽  
Urgent Problem ◽  
Combined Exposure

An urgent problem is the search for substances that can provide a protective effect in cases of DNA synthesis and repair disorders that arise as a result of side effects of cytostatic drugs used in the treatment of cancer. The aim of this work was to study the effect of 20 encoded amino acids in the presence of Cyclophosphane on the development of organotypic culture of rat liver tissue. The results obtained indicate that Cyclophosphane; which simulates the action of such cytostatic substances; inhibits cell proliferation in the liver tissue. It was also found that the encoded amino acids: asparagine; arginine; and glutamic acid; eliminate the inhibitory effect of Cyclophosphane in liver tissue culture. The growth zone of explants after combined exposure to Cyclophosphane (whose isolated action suppressed the growth zone) and these amino acids increased significantly and reached control values. Thus; the experimental data create the basis for the development of methods for the therapeutic use of the three studied amino acids for the removal of side effects in the treatment with cytostatic drugs.

PROTECTIVE EFFECT OF POLYPEPTIDE AND AMINO ACIDS ON THE DEVELOPMENT OF THE NERVOUS TISSUE CULTURE IN THE PRESENCE OF CYCLOPHOSPHAMIDE

2017 ◽  
pp. 22-26
Author(s):  
V. B. Dolgo-Saburov ◽  
N. I. Chalisova ◽  
L. V. Lyanginen ◽  
E. S. Zalomaeva
Keyword(s):  
Amino Acids ◽  
Cell Proliferation ◽  
Tissue Culture ◽  
Protective Effect ◽  
Organotypic Culture ◽  
Inhibitory Effect ◽  
Mustard Gas ◽  
Synthesis Inhibitor ◽  
Combined Administration ◽  
The Central Nervous System

In an organotypic culture, an investigation was conducted into combined effects of cyclophosphamide DNA as synthesis inhibitor used to model a resorptive action of mustard gas, and cortexin polypeptide or each of 20 encoded amino acids on the development of cell proliferation in cerebral cortex explants of the rat. The combined administration of cyclophosphamide together with cortexin or with each of the 20 encoded amino acids, except glycine, showed suppression of the cytostatic agent inhibitory effect. Thus, cortexin and amino acids have a protective effect on cell proliferation in the tissue culture of the central nervous system under the action of mustardlike substances.

Mechanisms of cellular proliferation stimulation under the effect of L-amino acids in the tissue culture of young and old rats

2014 ◽  
Vol 4 (1) ◽  
pp. 28-36
Author(s):  
A. Yu. Soloviev ◽  
N. I. Chalisova ◽  
I. A. Chernova ◽  
L. K. Shataeva ◽  
D. A. Sinyachkin
Keyword(s):  
Amino Acids ◽  
Tissue Culture ◽  
Cellular Proliferation ◽  
Old Rats

Effects of atrazine and alachlor on self-purification processes in receiving streams

1996 ◽  
Vol 33 (6) ◽  
pp. 181-187 ◽  
Author(s):  
Jana Zagorc-Koncan
Keyword(s):  
Adverse Effect ◽  
Acute Toxicity ◽  
Toxicity Test ◽  
Chronic Toxicity ◽  
Acute Toxicity Test ◽  
Organic Substances ◽  
Inhibiting Effect ◽  
Scenedesmus Subspicatus ◽  
Net Assimilation ◽  
Chlorophyll A Content

In recent years many waterways in Slovenia have been subjected to an increased loading with pesticides due to intensification of agriculture. The most widely used herbicides are atrazine and alachlor and they were detected in some rivers and even in ground water. Therefore the effects of atrazine and alachlor on selfpurification processes were investigated. The basic selfpurification processes studied were biodegradation of organic substances and photosynthesis and growth of algae. The inhibiting effect of pesticides on the process of biodegradation of organic pollutants was evaluated by the use of laboratory river model and mathematical modelling. The harmful impacts of pesticides on aquatic autotrophic organisms were assessed by measurement of net assimilation inhibition (24-h acute toxicity test) as well as growth inhibition - chlorophyll- a content (72-h chronic toxicity test) of algae Scenedesmus subspicatus. The results obtained demonstrate that atrazine and alachlor in concentrations found in our rivers have practically no effect on biodegrading heterotrophic organisms, while their adverse effect on algae is quite considerable.

Effect of arginine and its metabolites on the rat myocardium in an organotypic tissue culture

2007 ◽  
Vol 415 (1) ◽  
pp. 257-260 ◽  
Author(s):  
N. I. Chalisova ◽  
A. N. Zakutskii ◽  
A. I. Aniskina ◽  
S. V. Filippov ◽  
P. N. Zezyulin ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Rat Myocardium ◽  
Organotypic Tissue Culture ◽  
Organotypic Tissue

FURTHER STUDIES ON THE DUAL REQUIREMENT FOR PHENYLALANINE AND TYROSINE IN TISSUE CULTURE

1961 ◽  
Vol 39 (5) ◽  
pp. 925-932 ◽  
Author(s):  
Helen J. Morton ◽  
Joseph F. Morgan
Keyword(s):  
Amino Acids ◽  
Tissue Culture ◽  
Chick Embryo ◽  
Heart Cells ◽  
Present System ◽  
Chick Heart ◽  
High Concentrations ◽  
Chick Embryo Heart ◽  
Embryo Heart ◽  
Related Compounds

Seventeen structurally related compounds were tested for their ability to substitute for phenylalanine or tyrosine in the nutrition of chick embryo heart fragments. DL-Alanyl-DL-phenylalanine replaced phenylalanine. All other compounds had negligible effects, and most were toxic at high concentrations. β-Phenylserine, a phenylalanine antagonist, actually prolonged the survival of chick heart cells but only if both phenylalanine and tyrosine were present. Similarly, optimal reversal of β-phenylserine toxicity was dependent on the presence of both amino acids. Although phenylalanine and tyrosine are not interconvertible in the present system, it has been shown that three phenylalanine antagonists, p-fluorophenylalanine, β-2-thienylalanine, and β-phenylserine, can be identified by their relationship to tyrosine, rather than to phenylalanine.

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