PROTECTIVE EFFECT OF POLYPEPTIDE AND AMINO ACIDS ON THE DEVELOPMENT OF THE NERVOUS TISSUE CULTURE IN THE PRESENCE OF CYCLOPHOSPHAMIDE

2017 ◽  
pp. 22-26
Author(s):  
V. B. Dolgo-Saburov ◽  
N. I. Chalisova ◽  
L. V. Lyanginen ◽  
E. S. Zalomaeva
Keyword(s):  
Amino Acids ◽  
Cell Proliferation ◽  
Tissue Culture ◽  
Protective Effect ◽  
Organotypic Culture ◽  
Inhibitory Effect ◽  
Mustard Gas ◽  
Synthesis Inhibitor ◽  
Combined Administration ◽  
The Central Nervous System

In an organotypic culture, an investigation was conducted into combined effects of cyclophosphamide DNA as synthesis inhibitor used to model a resorptive action of mustard gas, and cortexin polypeptide or each of 20 encoded amino acids on the development of cell proliferation in cerebral cortex explants of the rat. The combined administration of cyclophosphamide together with cortexin or with each of the 20 encoded amino acids, except glycine, showed suppression of the cytostatic agent inhibitory effect. Thus, cortexin and amino acids have a protective effect on cell proliferation in the tissue culture of the central nervous system under the action of mustardlike substances.

Protective influence of coded amino acids on the development of liver tissue culture in the presence of cytostatic

2020 ◽  
pp. 48-53
Author(s):  
N. I. Chalisova ◽  
V. K. Kozlov ◽  
A. B. Mulik ◽  
E. P. Zatsepin ◽  
T. A. Kostrova
Keyword(s):  
Amino Acids ◽  
Tissue Culture ◽  
Side Effects ◽  
Liver Tissue ◽  
Organotypic Culture ◽  
Growth Zone ◽  
Inhibitory Effect ◽  
Cytostatic Drugs ◽  
Urgent Problem ◽  
Combined Exposure

An urgent problem is the search for substances that can provide a protective effect in cases of DNA synthesis and repair disorders that arise as a result of side effects of cytostatic drugs used in the treatment of cancer. The aim of this work was to study the effect of 20 encoded amino acids in the presence of Cyclophosphane on the development of organotypic culture of rat liver tissue. The results obtained indicate that Cyclophosphane; which simulates the action of such cytostatic substances; inhibits cell proliferation in the liver tissue. It was also found that the encoded amino acids: asparagine; arginine; and glutamic acid; eliminate the inhibitory effect of Cyclophosphane in liver tissue culture. The growth zone of explants after combined exposure to Cyclophosphane (whose isolated action suppressed the growth zone) and these amino acids increased significantly and reached control values. Thus; the experimental data create the basis for the development of methods for the therapeutic use of the three studied amino acids for the removal of side effects in the treatment with cytostatic drugs.

scholarly journals EFFECT OF AMINO ACID ON THE DEVELOPMENT SKIN TISSUE ORGANOTYPIC CULTURE

2017 ◽  
Vol 19 (3) ◽  
pp. 71-76
Author(s):  
Natal'ya I Chalisova ◽  
Aleksander E Korovin
Keyword(s):  
Adverse Effect ◽  
Amino Acids ◽  
Tissue Culture ◽  
Cellular Proliferation ◽  
Organotypic Culture ◽  
Growth Zone ◽  
Rat Skin ◽  
Inhibiting Effect ◽  
Combined Action ◽  
Organotypic Tissue

The effect of the 20 coded amino acids was investigated on the development of the processes of the proliferation in the organotypic tissue culture of rat skin. The amino acids lysine, arginine and glutamine acid at 0,05 ng/ml concentration stimulated the cellular proliferation in the growth zone of explants by 29-30%. The mustard-like agent cyclophosphane at 1 mg/ml concentration inhibited the cellular proliferation. The delay of this inhibiting effect of cyclophosphane was observed by the combined action of stimulating amino acids with the cyclophosphane. Thus, the amino acids with the charge radicals can be protectors of the cellular proliferation by the toxic effect of the cyclophosphane on the skin. This effect can be used for the treatment of the mustard injury of skin and for the delay of the adverse effect of cytostatic in oncology (1 figure, 1 table, bibliography: 28 refs).

scholarly journals Qualitative GC-MS Assessment of TCP and Tamorf Elimination in Rats

2010 ◽  
Vol 61 (1) ◽  
pp. 61-67
Author(s):  
Maja Petek ◽  
Ana Vrdoljak ◽  
Gordan Mršić
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Protective Effect ◽  
Standard Treatment ◽  
Adamantane Derivative ◽  
Cholinergic Receptors ◽  
Rat Urine ◽  
Combined Administration ◽  
The Central Nervous System ◽  
New Compounds

Qualitative GC-MS Assessment of TCP and Tamorf Elimination in RatsNerve agents are highly toxic organophosphorus (OP) compounds. They inhibit acetylcholinesterase (AChE), an enzyme that hydrolyses acetycholine (ACh) in the nervous system. Pathophysiological changes caused by OP poisonings are primarily the consequence of surplus ACh on cholinergic receptors and in the central nervous system. Standard treatment of OP poisoning includes combined administration of carbamates, atropine, oximes and anticonvulsants. In order to improve therapy, new compounds have been synthesised and tested. Tenocyclidine (TCP) and its adamantane derivative 1-[2-(2-thienyl)-2-adamantyl] morpholine (TAMORF) have shown interesting properties against soman poisoning. In this study, we developed a qualitative GC-MS method to measure elimination of TCP and TAMORF through rat urine in order to learn more about the mechanisms through which TCP protects an organism from OP poisoning and to determine the duration of this protective effect. GC-MS showed that six hours after treatment with TCP, rat urine contained only its metabolite 1-thienylcyclohexene, while urine of rats treated with TAMORF contained both TAMORF and its metabolites.

Protective effect of coded L-amino acids in cytostatic presence on the development of organotypic culture of tissues of different genesis

2021 ◽  
Vol 19 (2) ◽  
pp. 49-53
Author(s):  
Natalia Iosifovna Chalisova ◽  
Galina Anatolevna Ryzhak ◽  
Oleg Mikchailovich Ivko ◽  
Ecaterina Sergeevna Zalomaeva ◽  
Polina Nicolaevna Ivanova
Keyword(s):  
Amino Acids ◽  
Protective Effect ◽  
Organotypic Culture

scholarly journals Effect of Amino Acids on the Generation of Ginsenoside Rg3 Epimers by Heat Processing and the Anticancer Activities of Epimers in A2780 Human Ovarian Cancer Cells

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Jun Yeon Park ◽  
Pilju Choi ◽  
Dahae Lee ◽  
Taejung Kim ◽  
Eun Bee Jung ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Amino Acids ◽  
Cell Proliferation ◽  
Cancer Cells ◽  
Inhibitory Effect ◽  
Heat Processing ◽  
Ovarian Cancer Cells ◽  
Active Components ◽  
Anticancer Activities ◽  
Ginseng Extract

Ginsenosides are the active components ofPanax ginseng. Many research studies indicate that these deglycosylated, less-polar ginsenosides have better bioactivity than the major ginsenosides. In the present study, we sought to verify the enhanced anticancer effect ofP. ginsengextract after undergoing the Maillard reaction as well as elucidate the underlying mechanism of action. The effects of 9 amino acids were tested; among them, the content of 20(S)-Rg3 in the ginseng extract increased to more than 30, 20, and 20% when processed with valine, arginine, and alanine, respectively, compared with that after normal heat processing. The ginseng extract that was heat-processed with arginine exhibited the most potent inhibitory effect on A2780 ovarian cancer cell proliferation. Therefore, the generation of 20(S)-Rg3 was suggested to be involved in this effect. Moreover, the inhibitory effect of 20(S)-Rg3 on A2780 cell proliferation was significantly stronger than that of 20(R)-Rg3. Protein expression levels of cleaved caspase-3, caspase-8, caspase-9, and PARP in the A2780 ovarian cancer cells markedly increased, whereas the expression of BID decreased after 20(S)-Rg3 treatment. Therefore, we confirmed that the anticancer effects of the products of ginseng that was heat-processed with arginine are mediated mainly via the generation of the less-polar ginsenoside 20(S)-Rg3.

Effects of combinations of amino acids on cell proliferation in tissue culture of old rats

2013 ◽  
Vol 3 (1) ◽  
pp. 77-79 ◽  
Author(s):  
N. I. Chalisova ◽  
E. A. Kontsevaya ◽  
A. N. Zhekalov ◽  
D. A. Sinyachkin
Keyword(s):  
Amino Acids ◽  
Cell Proliferation ◽  
Tissue Culture ◽  
Old Rats
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