scholarly journals Response of immunocompetent cells of bone marrow and spleen of mouse males of several strains to stress and to pyrazine containing chemosignals

2012 ◽  
Vol 10 (2) ◽  
pp. 14-20 ◽  
Author(s):  
Eugene V Daev ◽  
Boris P Surinov ◽  
Anna V Dukelskaya
Keyword(s):  
Bone Marrow ◽  
Immune Suppression ◽  
Food Production ◽  
Bone Marrow Cells ◽  
Pyrazine Ring ◽  
Mouse Genotype ◽  
Dividing Cells ◽  
Different Strains ◽  
Mitotic Disturbances ◽  
Wide Usage

The quantity of antibody producing cells and mitotic disturbances in dividing bone marrow cells of mice were studied after exposure of animals to a physical stressor or various pyrazinecontaining chemosignals. Several different strains of mice were used. We demonstrate that immune suppression and destabilization of the chromosome apparatus in dividing cells depend on: а) mouse genotype and b) side chains position  in the pyrazine ring. Importance of this effects in the light of wide usage of pyrazine containing substances in perfume industry, food production and pharmacology is discussed.

scholarly journals Post-stress chemosignals affect cells from immunocompetent organs in laboratory mice of three inbred strains

2008 ◽  
Vol 6 (1) ◽  
pp. 27-33 ◽  
Author(s):  
Eugene V Daev ◽  
Boris P Surinov ◽  
Anna V Dukelskaya
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Physiological State ◽  
Inbred Strains ◽  
Laboratory Mice ◽  
Immunocompetent Organs ◽  
Dividing Cells ◽  
First Time ◽  
Mitotic Disturbances ◽  
Post Stress

Quantity of antibody producing cells and changes in bone marrow dividing cells of mouse males were studied after the exposure with chemosignals from intact or stressed donor mouse males. Inbred CBA, BALB/c and C57BL/6 strains were used. It is shown that excreted volatiles decrease quantity of antibody producing cells in spleen and at the same time raise the level of mitotic disturbances in bone marrow cells of recipient mice. Pheromone effect depends on genotype and physiological state of the recipients. For the first time we describe here the influence of mouse female pheromone 2,5-dimethylpyrazine on analyzed features. Biological meaning of the discovered effects is discussed.

Enhanced proliferation of functionally competent bone marrow cells in different strains of mice treated with swainsonine

2003 ◽  
Vol 3 (3) ◽  
pp. 445-455 ◽  
Author(s):  
Oladipo A Oredipe ◽  
Paulette M Furbert-Harris ◽  
Ibrahim Laniyan ◽  
William R Green ◽  
Sandra L White ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Different Strains ◽  
Marrow Cells

Biochemical changes and increased liver weight in bone marrow chimeras

1961 ◽  
Vol 200 (1) ◽  
pp. 102-106 ◽  
Author(s):  
A. L. Kretchmar ◽  
C. C. Congdon
Keyword(s):  
Bone Marrow ◽  
Weight Loss ◽  
Bone Marrow Cells ◽  
Liver Weight ◽  
Good Recovery ◽  
Female Donor ◽  
Total Body ◽  
Different Strains ◽  
Bone Marrow Chimeras ◽  
Marrow Cells

Female mice of two different strains were x-irradiated (950 or 900 r total-body) and then given 40 x 106 bone marrow cells from the same or a different strain of female donor mice. Irradiated mice lost weight during the 1st week after treatment. Mice given bone marrow cells showed good recovery except that when the cells were from a different strain there was a second weight loss persisting to the 35th day. The liver weight increased after treatment. The maximum was noted at 12 days. Glutamine and glutathione concentration in livers of treated mice was reduced at 12 days and the concentration of ß-aminoisobutyric acid and of ß-alanine was increased. The changes were greatest in animals given bone marrow cells of a different strain.

Adult stem cells from bone marrow (MSCs) isolated from different strains of inbred mice vary in surface epitopes, rates of proliferation, and differentiation potential

Blood ◽  
2004 ◽  
Vol 103 (5) ◽  
pp. 1662-1668 ◽  
Author(s):  
Alexandra Peister ◽  
Jason A. Mellad ◽  
Benjamin L. Larson ◽  
Brett M. Hall ◽  
Laura F. Gibson ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Adult Stem Cells ◽  
Bone Marrow Cells ◽  
Inbred Mice ◽  
Optimal Growth ◽  
Differentiation Potential ◽  
Murine Bone Marrow ◽  
Different Strains ◽  
Cell Adhesion Molecule 1

AbstractFor reasons that are not apparent, it has been difficult to isolate and expand the adult stem cells referred to as mesenchymal stem cells or marrow stromal cells (MSCs) from murine bone marrow. We developed a protocol that provides rapidly expanding MSCs from 5 strains of inbred mice. The MSCs obtained from 5 different strains of mice were similar to human and rat MSCs in that they expanded more rapidly if plated at very low density, formed single-cell–derived colonies, and readily differentiated into either adipocytes, chondrocytes, or mineralizing cells. However, the cells from the 5 strains differed in their media requirements for optimal growth, rates of propagation, and presence of the surface epitopes CD34, stem cell antigen-1 (Sca-1), and vascular cell adhesion molecule 1 (VCAM-1). The protocol should make it possible to undertake a large number of experiments with MSCs in transgenic mice that have previously not been possible. The differences among MSCs from different strains may explain some of the conflicting data recently published on the engraftment of mouse MSCs or other bone marrow cells into nonhematopoietic tissues.

scholarly journals Predator odor induces genome instability in the mouse bone marrow cells

2017 ◽  
Vol 15 (1) ◽  
pp. 4
Author(s):  
Timofey S Glinin ◽  
Polina A Starshova ◽  
Victoria A Shubina ◽  
Margarita V Anisimova ◽  
Anton A Bondarenko ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Genome Instability ◽  
Genetic Effect ◽  
Predator Odor ◽  
Mouse Bone Marrow ◽  
Predator Species ◽  
Genome Damage ◽  
Dividing Cells ◽  
Marrow Cells

Background. Long coevolution of prey and predator species of mammals creates specific mechanisms of their interaction, e. g. prey’s innate behavior aversive to the predator odor. However, little is known about genetic responses in the prey organism. We assessed genome instability of the bone marrow cells in mice affected by the cat’s odor influence, and proposed pathway of such action. Materials and methods. CBA mouse males were exposed to volatiles from adult cat urine for 2 or 24 hours. To estimate the genetic effect, ana-telophase method of chromosome aberration analysis and comet assay were used. The level of corticosterone was also measured after the exposure for 30 or 60 minutes. Results. The exposure to cat’s urine volatiles for 2 hours induced damage of DNA in bone marrow cells of the mouse males as was shown by the DNA comet analysis. The exposure for 24 hours elevated the frequency of chromosome aberrations in mitotically dividing cells at ana-telophase stage. No significant changes were found in the level of corticosterone in the peripheral blood. Conclusion. We have shown that volatile chemosignals from predator’s urine induce genomic instability in bone marrow cells of a prey. The hormonal pathway of such influence is still unknown. Intraorganismic paths leading to genome damage are discussed as well as far consequences of discovered effects.

Morphofunctional features of proliferating cells exposed to PSMA peptide

2021 ◽  
Author(s):  
N.A. Mikheeva ◽  
E.P. Drozhdina ◽  
N.A. Kurnosova
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Micronucleus Test ◽  
Laboratory Animals ◽  
Laboratory Mice ◽  
Proliferating Cells ◽  
Red Bone Marrow ◽  
Toxic Damage ◽  
Dividing Cells ◽  
Marrow Cells

The effect of the synthetic PSMA peptide on dividing cells of laboratory animals was studied. The experiment was carried out on male white laboratory mice of the BALB/c-line. The toxic effect of PSMA peptidi was evaluated at therapeutic (1.4 μg / kg of animal weight or 0.04 μg / animal) and subtoxic (140 μg / kg of animal weight or 4.0 μg / animal) doses. The cytotoxic effect of PSMA peptide on red bone marrow cells and cambial intestinal cells of the of laboratory mice was determined. A decrease in the proliferative activity of the colon crypt cells was revealed upon administration of a subtoxic dose of the PSMA peptide and there were no signs of toxic damage to the red bone marrow cells of animals. Key words: toxicity, proliferation, synthetic peptides, mitotic index, micronucleus test.

scholarly journals Development of hematopoietic spleen colonies in nonirradiated genetically normal mice

Blood ◽  
1977 ◽  
Vol 50 (6) ◽  
pp. 1121-1127 ◽  
Author(s):  
Y Kitamura ◽  
M Tamai ◽  
Y Miyano ◽  
M Shimada
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Erythroid Cell ◽  
Hematopoietic Stem ◽  
F1 Hybrid ◽  
C57bl Mice ◽  
Dividing Cells ◽  
Hybrid Mice ◽  
Suitable Environment ◽  
Marrow Cells

Abstract The question as to whether prior irradiation or injection of cytotoxic drugs is essential for the development of spleen colonies was examined in genetically normal mice. Mixtures of lymph node and bone marrow cells from C57BL mice were injected into (C57BL X CBA-T6T6) F1 hybrid mice without pretreatment. Hematopoietic nodules were observed in the spleens of F1 hybrid mice killed 18 days after injection. The average number of nodules increased linearly with increased numbers of injected bone marrow cells. Hematopoietic stem cells (CFU-S) and dividing cells in the nodules were shown to be of C57BL origin. Histologic examination showed that erythroid cell colonies predominated over granulocytic cell colonies. These results suggest that any kind of treatment that causes the depletion of CFU-S in the spleen of hosts would provide a suitable environment for the production of colonies by transplanted CFU-S.

scholarly journals PROLIFERATION OF DONOR MARROW AND THYMUS CELLS IN THE MYELOID AND LYMPHOID ORGANS OF IRRADIATED SYNGENEIC HOST MICE

1973 ◽  
Vol 137 (2) ◽  
pp. 543-546 ◽  
Author(s):  
Akikazu Takada ◽  
Yumiko Takada
Keyword(s):  
Bone Marrow ◽  
Lymph Nodes ◽  
Bone Marrow Cells ◽  
The Other ◽  
Host Bone ◽  
Donor Type ◽  
Dividing Cells ◽  
Spleen And Lymph Nodes ◽  
Thymus Cells ◽  
Marrow Cells

CBA/HT6T6 bone marrow cells (1 x 107) or CBA/H bone marrow cells (1 x 107) plus CBA/HT6T6 thymus cells (5 x 107) were injected intravenously into lethally (800 R) irradiated CBA/H mice. Chromosome analyses of dividing cells in the host lymphoid and myeloid organs were performed at intervals after irradiation. Donor marrow cells settled and proliferated in the host bone marrow, spleen, and lymph nodes soon after injection, but donor marrow cells did not proliferate in the host thymus until day 10; then host-type cells were quickly replaced by donor-type cells in the thymus by day 20. On the other hand, donor thymus cells settled and proliferated in the host thymus and lymph nodes soon after injection but they gradually disappeared from these organs. On day 20, a few donor-type dividing cells (of thymus origin) were found in the host lymphoid and myeloid organs.

scholarly journals Development of hematopoietic spleen colonies in nonirradiated genetically normal mice

Blood ◽  
1977 ◽  
Vol 50 (6) ◽  
pp. 1121-1127
Author(s):  
Y Kitamura ◽  
M Tamai ◽  
Y Miyano ◽  
M Shimada
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Erythroid Cell ◽  
Hematopoietic Stem ◽  
F1 Hybrid ◽  
C57bl Mice ◽  
Dividing Cells ◽  
Hybrid Mice ◽  
Suitable Environment ◽  
Marrow Cells

The question as to whether prior irradiation or injection of cytotoxic drugs is essential for the development of spleen colonies was examined in genetically normal mice. Mixtures of lymph node and bone marrow cells from C57BL mice were injected into (C57BL X CBA-T6T6) F1 hybrid mice without pretreatment. Hematopoietic nodules were observed in the spleens of F1 hybrid mice killed 18 days after injection. The average number of nodules increased linearly with increased numbers of injected bone marrow cells. Hematopoietic stem cells (CFU-S) and dividing cells in the nodules were shown to be of C57BL origin. Histologic examination showed that erythroid cell colonies predominated over granulocytic cell colonies. These results suggest that any kind of treatment that causes the depletion of CFU-S in the spleen of hosts would provide a suitable environment for the production of colonies by transplanted CFU-S.

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