Morphofunctional features of proliferating cells exposed to PSMA peptide

2021 ◽  
Author(s):  
N.A. Mikheeva ◽  
E.P. Drozhdina ◽  
N.A. Kurnosova
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Micronucleus Test ◽  
Laboratory Animals ◽  
Laboratory Mice ◽  
Proliferating Cells ◽  
Red Bone Marrow ◽  
Toxic Damage ◽  
Dividing Cells ◽  
Marrow Cells

The effect of the synthetic PSMA peptide on dividing cells of laboratory animals was studied. The experiment was carried out on male white laboratory mice of the BALB/c-line. The toxic effect of PSMA peptidi was evaluated at therapeutic (1.4 μg / kg of animal weight or 0.04 μg / animal) and subtoxic (140 μg / kg of animal weight or 4.0 μg / animal) doses. The cytotoxic effect of PSMA peptide on red bone marrow cells and cambial intestinal cells of the of laboratory mice was determined. A decrease in the proliferative activity of the colon crypt cells was revealed upon administration of a subtoxic dose of the PSMA peptide and there were no signs of toxic damage to the red bone marrow cells of animals. Key words: toxicity, proliferation, synthetic peptides, mitotic index, micronucleus test.

scholarly journals METHODS FOR STUDYING AND ASSESSING CYTOGENETIC CHANGES IN BONE MARROW CELLS OF LABORATORY ANIMALS RECEIVING GENE-MODIFIED

2021 ◽  
Vol 58 (2) ◽  
pp. 4988-4995
Author(s):  
N.A.Nuraliyev, A.Kh.Allanazarov, M.S.Gildiyeva, D.R.Sobirova
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Normal Karyotype ◽  
Laboratory Animals ◽  
Control Group ◽  
Red Bone Marrow ◽  
Comparative Aspect ◽  
Mitotic Pathology ◽  
Cytogenetic Changes ◽  
Marrow Cells

The aim was to study cytogenetic changes in bone marrow cells of laboratory animals that received and did not receive a genetically modified (GM) product (GM-soy) in a comparative aspect. It was found that in the group of white outbred rats that received GM-soy, cytogenetic changes were found in the cells of the red bone marrow, expressed in partial inhibition of cell proliferation due to the cytotoxic effect. They also had polyploidy (5.35%) and aneuploidy (5.35%) in 10.7% of cases. In addition, this group contained cells with mitotic pathology - chromosome scattering, chromosome pulverization, impaired spiralization and despiralization of chromosomes, premature spiralization of chromosomes, delayed mitosis at the prophase stage. In the group of animals that did not receive soy with and without GM in the cells, there were metaphase plates with a normal karyotype. The mitotic activity of bone marrow cells in the control group was higher (MI - 9%) than in the first (MI - 3%) and the second group (MI - 5%).  

scholarly journals Toxic effects of various zinc dosages on poultry hemopoiesis

2020 ◽  
Vol 164 ◽  
pp. 06011
Author(s):  
Aleksandr Vishnyakov
Keyword(s):  
Bone Marrow ◽  
Broiler Chickens ◽  
Bone Marrow Cells ◽  
Marrow Cell ◽  
Ultrastructural Level ◽  
Cell Nuclei ◽  
Red Bone Marrow ◽  
Zinc Salts ◽  
Destructive Processes ◽  
Marrow Cells

The aim of the study was to determine the main patterns of the manifestation of the toxic effect of zinc chloride on the cells of the red bone marrow in the first three days after exposure. Our experiment involved broiler chickens of the “Smena-7” cross. The work presents new data on the effects of zinc salts in doses of 40 mg/kg and 60 mg/kg on the hemopoietic cells of the red bone marrow of birds at the ultrastructural level. Thus, the results of the study showed zinc poisoning of chickens even within 1-3 days led to damage to bone marrow cells. The cytoplasm of blood-forming and stromal cells of the bone marrow detects mostly signs of destructive processes, which are amplified as the duration and the dose of exposure increase. At the same time, the structure of cell nuclei often gets changed in the bone marrow. Emerging morphological signs indicate a decrease in the transcription of ribosomal RNA genes of bone marrow cells. In the bone marrow, the number of basophil erythrokaryocytes increases and the number of hemoglobinized forms of red blood cells decreases. Zinc mainly causes disorders of morpho-functional structures in erythroblasts and mature cells of other bone marrow cell lines.

Pathways in the development of liver macrophages: alternative precursors contained in populations of lymphocytes and bone-marrow cells

1968 ◽  
Vol 169 (1016) ◽  
pp. 307-327 ◽  
Keyword(s):  
Bone Marrow ◽  
Thoracic Duct ◽  
Bone Marrow Cells ◽  
Marker Chromosome ◽  
Proliferating Cells ◽  
Liver Macrophages ◽  
Bone Marrow Precursor ◽  
Liver Macrophage ◽  
Donor Cells ◽  
Marrow Cells

The origin of dividing liver macrophages during states of intense reticulo-endothelial stimulation has been studied in mice by means of the T 6 marker chromosome. The cells were isolated for cytological analysis by means of Garvey’s technique of collagenase and trypsin digestion. During the proliferative phase of graft-versus-host ( GVH ) reaction in the strain combination C 57BL → (C57BL x CBA-T6T6)F 1 , practically all liver macrophages in mitosis were of donor karyotype, even when relatively pure suspensions of thoracic duct small lymphocytes were used as the donor cells. Several lines of evidence established that the dividing cells analysed were part of a macrophage response. The isolated cells in mitosis had macrophage characteristics which reflected the cell proliferation examined in histological sections. This proliferation was largely restricted to the liver sinusoids and to cells with phagocytic properties. The same proportion of these cells appeared to be actively phagoctyic before their arrest in metaphase by Colcemid during GVH reaction as was found in normal mice. Furthermore, more than 70% of the liver sinusoidal cells which incorporated 3 H -thymidine were demonstrably phagocytic before and/or after labelling. Liver macrophage proliferation was greatly depressed by splenectomy 24 h after injection of donor cells, although cells of donor karyotype were still predominant. Similar techniques have been applied to syngeneic radiation chimaeras—( CBA x CBA-T6T6 ) F 1 mice ‘repopulated’ with CBA- T6T6 lymphocytes and CBA bone marrow. When Corynebacterium parvum vaccine was applied as a stimulant, two-thirds of dividing liver macrophages were found to be of lymphocyte origin and one-third or less derived from a precursor in bone marrow cells. Using partial hepatectomy to stimulate macrophage proliferation in these chimaeras, however, it was found that the overwhelming majority were derived from the bone-marrow precursor. The phagocytic property of the majority of proliferating cells was established by combined colloid and 3 H-thymidine labelling. It is concluded that liver macrophages derived from either of two different precursors in populations of recirculating lymphocytes and bone marrow cells respectively can proliferate preferentially, according to the nature of the reticulo-endothelial stimulus. Evidence from a variety of sources supports the contention that the bone-marrow precursor cell represents the major source of ‘normal’ macrophages. Whether the precursor amongst thoracic duct cells is identifiable with any previously recognized category of lymphocyte is not yet known. Its utilization has only been detected so far during conditions of intense reticulo-endothelial stimulation.

scholarly journals Micronucleus Test of DHU001, a Polyherbal Formula, in Bone Marrow Cells of Male ICR Mice

2009 ◽  
Vol 25 (4) ◽  
pp. 225-230 ◽  
Author(s):  
Seong-Soo Roh ◽  
Hyeung-Sik Lee ◽  
Sae-Kwang Ku
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Micronucleus Test ◽  
Icr Mice ◽  
Marrow Cells

scholarly journals In vivo genotoxicity of treated water containing the cylindrospermopsin-producer Cylindrospermopsis raciborskii

2014 ◽  
Vol 12 (3) ◽  
pp. 474-483 ◽  
Author(s):  
A. L. Fonseca ◽  
J. Da Silva ◽  
E. A. Nunes ◽  
S. M. F. O. Azevedo ◽  
R. M. Soares
Keyword(s):  
Bone Marrow ◽  
Comet Assay ◽  
Bone Marrow Cells ◽  
Micronucleus Test ◽  
Liver Cells ◽  
Cylindrospermopsis Raciborskii ◽  
Producer Strain ◽  
Treated Water ◽  
Marrow Cells

Cylindrospermopsin (CYN) is an alkaloid commonly produced by some cyanobacteria that has been implicated in outbreaks of human illness. The aim of this study was to investigate the genotoxicity of Cylindrospermopsis raciborskii cellular content (including CYN) and its byproducts resulting from chlorination during water treatment. DNA damage in blood and liver cells was analysed by the comet assay and micronucleus test (MN). Mice were injected intraperitoneally with the following treatments: (a) physiological saline, (b) treated water, (c) treated water plus C. raciborskii extract (CYN producer strain, CYPO-011 K), (d) C. raciborskii extract (CYN producer strain, CYPO-011 K), (e) C. raciborskii extract (CYN non producer strain), and (f) treated water plus C. raciborskii extract (CYN non producer strain) extract. After 48 h, samples were taken to perform tests (blood and liver cells to the comet assay and bone marrow to MN test). The CYPO-011 K had a genotoxic and mutagenic effects on liver and bone marrow cells. The group that received chlorine-treated water plus CYPO-011 K also exhibited genotoxic effects in the liver, as well as in the blood, and a mutagenic effect in blood marrow cells. The results emphasise the need of improving CYN monitoring in waters bodies in order to reduce the risk of human exposure.

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