The roles of genes of ksenobiotics biotransformation in the development of predisposition to the toxic heoatitis in workers workers exposed to hepthyle and ethylebenzene-styrene.

Introduction: The aim of this study was to estimate the predisposition of influencing possible factors causing chemical induced abnormal liver function on the basis of studying genes encoding xenobiotic metabolizing enzymes. Methods: Genotyping of CYP1A1, CYP2D6, CYP2E1, EPHX1, NAT2 was performed using polymerase chain reaction and restriction fragment length polymorphism on peripheral leucocyte DNA from 73 incident cases of toxic hepatitis, 163 «groups of risk» on development of a toxic hepatitis, 94 healthy workers and 335 controls.Results and conclusions: No significant association was found between a reference group and petrochemical workers when CYP1A1, CYP2D6, CYP2E1, EPHX1 genotypes were included in the analyses. Among workers was observed the increasing of frequency of a combination *4/*4 genes NAT2 compared with control group. Among the patients with a professional toxic hepatitis are established genetic markers of predisposition to development the disease: Ile/Val gene CYP1A1, Tyr/His gene EPHX1; combinations *4/*7 genes NAT2; and as slow phenotype microsomal epoxide hydrolase; combinations of genotypes IleVal/C1C1 of genes CYP1A1 and CYP2E1; combinations of slow phenotypes microsomal epoxide hydrolase and N-acetyltransferase-2. Our results suggest that genotype Ile/Ile of gene CYP1A1; genotype Tyr/Tyr of gene EPHX1; and as a normal phenotype microsomal epoxide hydrolase; a combination of genotypes IleIle/C1C1 of genes CYP1A1 and CYP2E1; a combination of genotypes IleIle/C1C1/CC/N of genes CYP1A1, CYP2E1, CYP2D6 and a normal phenotype microsomal epoxide hydrolase are protective variants. This study demonstrates a significant combined effect of phase I and phase II polymorphisms on the predisposition of professional pathology at workers exposed to hepthyle and ethylebenzene-styrene.

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Polymorphisms of Microsomal Epoxide Hydrolase and Glutathione S-transferase P1 in a Male Turkish Population

International Journal of Toxicology ◽

10.1080/10915810601118222 ◽

2007 ◽

Vol 26 (1) ◽

pp. 41-46 ◽

Cited By ~ 10

Author(s):

Ahmet O. Ada ◽

H. Sinan Suzen ◽

Mumtaz Iscan

Keyword(s):

Gene Polymorphisms ◽

Epoxide Hydrolase ◽

Turkish Population ◽

Microsomal Epoxide Hydrolase ◽

Glutathione S Transferase ◽

Metabolizing Enzymes ◽

Genotype Frequencies ◽

Pcr Rflp ◽

Genes Encoding ◽

Gstp1 Gene

Polymorphic genes encoding drug-metabolizing enzymes may account for interindividual differences in certain types of diseases especially cancer. In this study, microsomal epoxide hydrolase (EPHX1) and glutathione S-transferase P1 (GSTP1) gene polymorphisms were determined among 133 healthy males of a Turkish population. Frequencies of EPHX1 and GSTP1 gene polymorphisms were determined by using the polymerase chain reaction–restriction fragment length polymorphism (PCR/RFLP) method. The observed genotype frequencies of EPHX1 exon 3 were Tyr113Tyr:50.4%, Tyr113His: 42.1%, His113His: 7.5% and EPHX1 exon 4 were His139His: 69.2%, His139Arg: 28.6%, Arg133Arg: 2.2%. GSTP1 exon 5 genotype frequencies were Ile105Ile: 58.7%, Ile105Val: 35.3%, Val105Val: 6.0% and GSTP1 exon 6 genotype frequencies were Ala114Ala: 85.0%, Ala114Val: 14.3%, Val114Val: 0.7%. These results reveal that the frequencies of EPHX1 and GSTP1gene polymorphisms in a small sampling of males within a Turkish population are similar to European Caucasian populations.

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Interaction between Cytochrome P450 and Other Drug-Metabolizing Enzymes: Evidence for an Association of CYP1A1 with Microsomal Epoxide Hydrolase and UDP-Glucuronosyltransferase

Biochemical and Biophysical Research Communications ◽

10.1006/bbrc.2000.3076 ◽

2000 ◽

Vol 273 (3) ◽

pp. 1048-1052 ◽

Cited By ~ 40

Author(s):

Ken-ichiro Taura ◽

Hideyuki Yamada ◽

Yukiko Hagino ◽

Yuji Ishii ◽

Masa-aki Mori ◽

...

Keyword(s):

Cytochrome P450 ◽

Epoxide Hydrolase ◽

Microsomal Epoxide Hydrolase ◽

Drug Metabolizing Enzymes ◽

Metabolizing Enzymes ◽

Udp Glucuronosyltransferase

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The Effects of the "XGTQ" Medicine in Treating Cirrhosis in Wistar Rats

Current Traditional Medicine ◽

10.2174/2215083806999201113120427 ◽

2020 ◽

Vol 06 ◽

Author(s):

Ngan Nguyen Hoang ◽

Thang Duong Minh ◽

Tuan Anh Hoang ◽

Son Le Ngoc Bich ◽

Duong Nguyen Huu ◽

...

Keyword(s):

Wistar Rats ◽

Reference Group ◽

Control Group ◽

Plain Water ◽

Group 4 ◽

White Rats ◽

Collagen Accumulation ◽

Activity Of Enzymes ◽

Group 3 ◽

Group 1

Objectives: Evaluate the effects of "XGTQ" in the treatment of cirrhosis induced by Carbon tetrachloride (CCL4) in combination with alcohol and high-fat diet on Wistar rats. Materials and methods: Cirrhosis on white rats was induced by subcutaneously injecting CC14 at an initial dose of 5,0ml/kg, followed by 1,2ml/kg once a week in 10 weeks. Then, fed with synthetic food, added 20% fat, and 0.05% cholesterol and iron oxalate. Rats were administered every day with plain water and 1 day with water mixed with 30% ethanol. The rats were randomly divided into 5 groups and given distilled water (group 1 and 2 or control group), silymarin (group 3 or reference group) or the "XGTQ" drug extract (group 4, 5) for 4 weeks. Collected blood for biochemical test and liver were dissected to evaluate weight, morphology and quantified 4-hydroxyproline to evaluate fibrosis and collagen accumulation. Results: In cirrhotic wistar rats, "XGTQ" drug at 19.6 g/kg/24h and 58.8 g/kg/24h showed the ability of reducing the activity of enzymes AST, ALT in the blood (p<0.01), increasing plasma albumin and decreasing prothrobin time (p<.05); improving physical condition, macroscopic and microscopic images of H&E-stained liver; decreasing the concentration of hydroxyproline in the liver and reducing the level of cirrhosis on the masson-stained templates. The effects of "XGTQ" increased with the dose, and was equivalent to silymarin at the dose of 70 mg/kg/24h. Conclusion: The extract of "XGTQ" drug is effective in treating cirrhosis in Wistar rats.

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The covalent structure of hepatic microsomal epoxide hydrolase. I. Isolation and characterization of the cyanogen bromide fragments.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(17)43527-7 ◽

1984 ◽

Vol 259 (2) ◽

pp. 791-796 ◽

Cited By ~ 6

Author(s):

F S Heinemann ◽

J Ozols

Keyword(s):

Epoxide Hydrolase ◽

Cyanogen Bromide ◽

Microsomal Epoxide Hydrolase ◽

Isolation And Characterization ◽

Covalent Structure

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The Assessment of IL-21 and IL-22 at the mRNA Level in Tumor Tissue and Protein Concentration in Serum and Peritoneal Fluid in Patients with Ovarian Cancer

Journal of Clinical Medicine ◽

10.3390/jcm10143058 ◽

2021 ◽

Vol 10 (14) ◽

pp. 3058

Author(s):

Aleksandra Mielczarek-Palacz ◽

Celina Kruszniewska-Rajs ◽

Marta Smycz-Kubańska ◽

Jarosław Strzelczyk ◽

Wojciech Szanecki ◽

...

Keyword(s):

Ovarian Cancer ◽

Peritoneal Fluid ◽

Tumor Tissue ◽

Mrna Level ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Expression Of Genes ◽

Genes Encoding ◽

First Time ◽

Ovarian Serous Cancer

The aim of the analysis was for the first time to assess the expression of genes encoding IL-21 and IL-22 at the mRNA level in ovarian tumor specimens and the concentration of these parameters in serum and peritoneal fluid in patients with ovarian serous cancer. The levels of IL-21 and IL-22 transcripts were evaluated with the use of the real-time RT-qPCR. Enzyme-linked immunosorbent assay (ELISA) was used to determine the concentration of proteins. Quantitative analysis of IL-21 gene mRNA in the tumor tissue showed the highest activity in the G1 degree of histopathological differentiation and was higher in G1 compared to the control group. The concentration of IL-21 and IL-22 in the serum and in the peritoneal fluid of women with ovarian cancer varied depending on the degree of histopathological differentiation of the cancer and showed statistical variability compared to controls. The conducted studies have shown that the local and systemic changes in the immune system involving IL-21 and IL-22 indicate the participation of these parameters in the pathogenesis of ovarian cancer, and modulation in the IL-21/IL-22 system may prove useful in the development of new diagnostic and therapeutic strategies used in patients, which require further research.

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