scholarly journals Functional properties of mesenchymal multipotent stromal stem cells obtained from bone marrow of patients with liver cirrhosis

2014 ◽  
Vol 95 (5) ◽  
pp. 669-674
Author(s):  
B A Agaev ◽  
R M Agaev ◽  
A G Popandopulo ◽  
R E Dzhafarli
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Liver Cirrhosis ◽  
Functional Properties ◽  
Primary Cultures ◽  
Small Cells ◽  
Viral Etiology ◽  
Phenotypic Properties ◽  
Autologous Mesenchymal Stem Cells

Aim. To study of the functional properties of autologous mesenchymal multipotent stem cells in patients with cirrhosis of different etiologies. Methods. Studied were the functional and phenotypic properties of autologous mesenchymal stem cells derived from the bone marrow in 35 patients (26 men and 9 women) with cirrhosis of the liver at the age of 19-53 years. Among the patients studied viral etiology was diagnosed in 18 (51.4%), alcohol - in 13 (37.1%), autoimmune - in 4 (11.4%) patients with liver cirrhosis. Results. Autologous mesenchymal stem cells from all of the observed patients were able to divide. The highest yield and the doubling rate of cell populations were observed in patients with viral cirrhosis. Comparative morphometric study of primary cultures derived stem cells in patients with autoimmune and alcoholic hepatitis, showed the presence of relatively small cells (less than 20 microns), which make up about 30% of the total number of cells. At the same time, in bone marrow derived cell cultures of patients with viral etiology of the disease the number of small cells was 1.5 times greater which makes approximately 49% of the total cell population. The vast majority of cultured stem cells, regardless of the etiology of cirrhosis express specific for these cells «stromal» markers - CD73, CD90 and CD105. Autologous mesenchymal stem cells isolated from bone marrow of patients with alcoholic liver cirrhosis characterized by the most pronounced pro-inflammatory, immunoregulatory potential. Conclusion. Functional and phenotypic properties of autologous mesenchymal stem cells in various forms of cirrhosis are different which requires adequate cultivation and correction before their transplantation.

Molecular and Cellular Functions Distinguish Superior Therapeutic Efficiency of Bone Marrow CD45 Cells Over Mesenchymal Stem Cells in Liver Cirrhosis

Stem Cells ◽  
2015 ◽  
Vol 34 (1) ◽  
pp. 135-147 ◽  
Author(s):  
Prakash Baligar ◽  
Snehasish Mukherjee ◽  
Veena Kochat ◽  
Archana Rastogi ◽  
Asok Mukhopadhyay
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Liver Cirrhosis ◽  
Cellular Functions ◽  
Therapeutic Efficiency

scholarly journals Autologous mesenchymal stem cells in treatment of liver cirrhosis: evaluation of effectiveness and visualization method

2020 ◽  
Vol 5 (3) ◽  
pp. 197-203
Author(s):  
Inna E. Kotkas ◽  
Natella I. Enukashvili ◽  
Shamil M. Asadulayev ◽  
Anna V. Chubar
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Liver Cirrhosis ◽  
Cell Therapy ◽  
Clinical Observation ◽  
Health Assessment ◽  
Subjective Health ◽  
Oxide Nanoparticles ◽  
Cell Structures ◽  
Autologous Mesenchymal Stem Cells

Through clinical observation, we present an assessment of the autologous mesenchymal stem cells effectiveness in treatment of liver cirrhosis of alimentary etiology. In order to determine the localization of the implanted cell structures, the stem cells were previously labeled with iron (II, III) oxide nanoparticles (IONPs). Further MRI visualization helped to detect the cell structures stained with iron oxide nanoparticles in the human body. In 6 months after the cell therapy, the patient underwent clinical and biochemical blood tests, MEGX test, elastography and subjective health assessment test. The tests data analysis revealed the improvement of the values of all examined parameters after the cell treatment. Also in 6 and 12 months after the treatment, a liver biopsy was performed from the area where the implanted stem cells were visualized. In histological examination of liver bioptates obtained from the area of MSC transplantation, the largest number of stained cells was observed in liver micronodes, as well as at the boundaries of micronodes and fibrous septa. A portion of the bioptate obtained in 12 months after transplantation was used to produce primary cell cultures. Before the first re-seeding of the cultures, cell colonies of both fibroblast-like morphology and epithelial were detected in them. Both types of colonies contained the particles. Conducting the cell therapy to a patient with liver cirrhosis of alimentary etiology contributed to improving the laboratory and instrumental examinations indicators. The patient had come through the treatment procedure satisfactorily, no complications were registered.

The effectiveness of autologous mesenchymal stem cells in the treatment of liver cirrhosis and the method of their visualization in the patient’s body

2020 ◽  
Vol 22 (3) ◽  
pp. 35-40
Author(s):  
I. E. Kotkas ◽  
N. I. Enukashvili ◽  
Sh. M. Asadulayev ◽  
A. V. Chubar’
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Liver Cirrhosis ◽  
Cell Therapy ◽  
Liver Tissue ◽  
Ultrasound Examination ◽  
Test Results ◽  
Resonance Imaging ◽  
Cell Structures ◽  
Autologous Mesenchymal Stem Cells

Abstract. The effectiveness of the influence of autologous mesenchymal stem cells on the function of liver tissue in liver cirrhosis of alimentary etiology is considered, as well as the possibility of visualization of the introduced cell structures with subsequent cytological confirmation. To be able to track autologous mesenchymal stem cells introduced in the patients body, they were labeled with iron oxide nanoparticles. Visualization of the introduced cell structures was performed using magnetic resonance imaging and ultrasound examination of the liver. 6 months after the cell therapy, clinical and biochemical blood tests, lidocaine test results, elastography indicators were evaluated, and the dynamics of hepatic encephalopathy was evaluated. The effectiveness of using autologous mesenchymal stem cells was evidenced by the improvement of the above indicators. Also, 6 months after treatment, a liver tissue biopsy was performed from the sites of fixation of previously introduced cell structures. Histological examination revealed the largest number of labeled cells in the liver micro-nodes, as well as at the borders of micro-nodes and fibrous septa. The use of cell therapy in a patient suffering from liver cirrhosis of alimentary etiology helped to improve the indicators of laboratory and instrumental research methods. No complications were detected during the procedure.

Resveratrol pretreatment enhanced homing of SDF-1α-preconditioned bone marrow-derived mesenchymal stem cells in a rat model of liver cirrhosis

2017 ◽  
Vol 119 (3) ◽  
pp. 2939-2950 ◽  
Author(s):  
Mehrdad Hajinejad ◽  
Parichehr Pasbakhsh ◽  
Ameneh Omidi ◽  
Keywan Mortezaee ◽  
Saied Nekoonam ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Liver Cirrhosis ◽  
Rat Model
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