Effect of the ointment based on benzosulfonate 1-ethyl-3-methyl-4,5-bis(N-methylcarbomoyl) imidazolium on wound healing in thermal skin damage in rats

AIM: Of the investigation was to assess the therapeutic efficacy of ointment based on benzosulfonate 1-ethyl-3-methyl-4,5-bis(N-methylcarbomoyl) imidazolium, an imidazol derivative, on healing of a thermal skin damage (burn wound) in rats. MATERIALS AND METHODS: In experiments on 150 male rats weighing 180200 g, the wound-healing effect of an ointment based on benzosulfonate 1-ethyl-3-methyl-4,5-bis(N-methylcarbomoyl) imidazolium (IEM-1181) after experimental thermal skin damage was studied. The ointment containing 10% of the compound IEM-1181 was prepared by the pharmacopoeial method based on lanolin. The control was the placebo effect (the basis of the ointment). For comparison, we used Solcoseril (ointment, Solco, Switzerland). Thermal damage to the skin of rats under ether anesthesia was carried out with a special device consisting of a metal plate and a temperature controller. The surface of the plate with a diameter of 1,5 cm was heated for 15 minutes to a temperature of 80С, and then applied to the pre-cut skin of the rats back (from the scapula caudally) for 15 seconds. After 24 hours, the skin of the animals developed damage to the epidermis and partially underlying dermis, corresponding to a grade II burn. The dynamics of changes in the burn wound was evaluated by 5, 10, 15, 20, 30, 40, 45, 50, 60, 70 days along the length of the contour bounding the affected area, using a curvimeter. RESULTS: It was found that 1-ethyl-3-methyl-4,5-bis(N-methylcarbomoyl) imidazolium benzosulfonate has a wound-healing effect in thermal skin damage in rats. With topical application of the ointment containing the test compound, the time of complete healing of the wound formed at the site of the burn defect was reduced by 30%, healing took place without signs of inflammation, with the formation of an elastic scar. CONCLUSIONS: Based on the conducted studies, it can be concluded that the ointment based on benzosulfonate 1-ethyl-3-methyl-4,5-bis(N-methylcarbomoyl) it has a wound-healing effect in thermal damage to the skin comparable in terms of the duration and degree of healing of burn defects with Solcoseril and can be the drug of choice for the treatment of grade II burns.

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Burn Wound Healing Effect and Hair Growth Promoting Activity of Lawsonia inermis L. and Honey in Oryctolagus cuniculus Rabbits

OnLine Journal of Biological Sciences ◽

10.3844/ojbsci.2016.82.89 ◽

2016 ◽

Vol 16 (2) ◽

pp. 82-89

Author(s):

Zouhir Djerrou ◽

Imane Mokhbi ◽

Khadidja Saci Hadef ◽

Noudjoud Boutobza ◽

Saida Bouzeguine ◽

...

Keyword(s):

Wound Healing ◽

Hair Growth ◽

Oryctolagus Cuniculus ◽

Lawsonia Inermis ◽

Burn Wound ◽

Healing Effect ◽

Burn Wound Healing ◽

Wound Healing Effect ◽

Growth Promoting

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Creation of Polymer Hydrogel Dressings with Herbal Medicinal Substance "Alkhydin" and their Properties

Eurasian Chemico-Technological Journal ◽

10.18321/ectj503 ◽

2017 ◽

Vol 19 (1) ◽

pp. 57

Author(s):

G. Temirkhanova ◽

G. Burasheva ◽

Zh. Abilov ◽

G. Irmukhametova ◽

A. Mun ◽

...

Keyword(s):

Wound Healing ◽

Polymer Network ◽

Dimensional Structure ◽

Initial Reaction ◽

Medicinal Substance ◽

Burn Wound ◽

Healing Effect ◽

Wound Healing Effect ◽

Herbal Medicinal

New hydrogel dressings containing the herbal medicinal substance "Alkhydin", obtained from the Kirghiz (Alhagi kirghisorum Schrenk), grown in Kazakhstan, were obtained by radiation irradiation of the initial reaction mixture (IRM). Poly-<br />N-vinylpyrrolidone (PVP) with a molecular weight (MM) of 1 ppm was used to prepare hydrogel dressings as the main gelling polymer for IRM, agar-agar and lowmolecular polyethylene glycol MM = 600 were used as auxiliary substances. The main regularities of the formation of the three-dimensional structure of PVP under conditions of irradiation of IRM are investigated. It is shown that with increasing PVP in IRM, as well as irradiation dose, the yield of gel fraction increases and the degree of swelling decreases. Obviously, these effects are due to an increase in the degree of cross-linking of the polymer network formed. For the obtained hydrogel dressings containing the herbal medicinal substance Alkhydin, cytotoxic effects on the culture of mouse embryonic fibroblasts obtained by primary trypsinization were studied. The results obtained in this case testify to the absence of a cytotoxic effect on the cell culture. In conditions "in vivo" in rats using the model of thermal skin burn, the wound healing effect of hydrogel dressings with "Alkhydin" was studied. It is shown that such bandages show a pronounced wound-healing effect. This is evidenced by a higher rate of reduction in the area of the burn wound treated with a hydrogel dressing compared to the control.

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Wound healing effect benzosulfonate 1-ethyl-3-methyl-4,5-bis(n-methylcarbamoyl) imidazolium

Reviews on Clinical Pharmacology and Drug Therapy ◽

10.17816/rcf183229-235 ◽

2020 ◽

Vol 18 (3) ◽

pp. 229-235

Author(s):

Lydmila K. Khnychenko ◽

Elena N. Selina ◽

Olga M. Rodionova ◽

Levon B. Piotrovskiy ◽

Petr D. Shabanov

Keyword(s):

Wound Healing ◽

Healing Process ◽

Experimental Models ◽

Skin Wound ◽

Complete Healing ◽

Male Rats ◽

Skin Wounds ◽

Skin Wound Healing ◽

Healing Effect ◽

Wound Healing Effect

Materials and methods. In experiments on 180 male rats weighing 180200 g, the wound-healing effect of derivatives of imidazole (IEM-1181) was evaluated as a 10% ointment on models of aseptic full-layer linear and planar skin wounds. Results. It was found that the compound IEM-1181 in the form of 10% ointment has a pronounced on skin wound healing effect, manifested in the qualitative features of the regenerative healing process. With local application of the ointment containing the tested compound, the strength of the tissue formed on the site of a full-layer linear skin wound was increased when its edges were stretched, and the healing time of full-layer planar wounds was reduced. The morphological picture of the tissue formed at the site of the wound defect corresponded to complete healing by primary tension with complete epithelization of the surface and squamous cell differentiation of the epithelial regenerate without signs of inflammation and scar formation. Conclusion. The results of the study on experimental models of full-layer linear and planar skin wounds indicate that the wound healing effect of the tested ointment is due to the anti-inflammatory activity of the IEM-1181 compound

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Burn-Wound Healing Effect of Gelatin/Polyurethane Nanofiber Scaffold Containing Silver-Sulfadiazine

Journal of Biomedical Nanotechnology ◽

10.1166/jbn.2013.1509 ◽

2013 ◽

Vol 9 (3) ◽

pp. 511-515 ◽

Cited By ~ 57

Author(s):

Dong Nyoung Heo ◽

Dae Hyeok Yang ◽

Jung Bok Lee ◽

Min Soo Bae ◽

Jung Ho Kim ◽

...

Keyword(s):

Wound Healing ◽

Silver Sulfadiazine ◽

Burn Wound ◽

Healing Effect ◽

Nanofiber Scaffold ◽

Burn Wound Healing ◽

Wound Healing Effect

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Novel Sorghum bicolor (L.) seed polysaccharide structure, hemolytic and antioxidant activities, and laser burn wound healing effect

International Journal of Biological Macromolecules ◽

10.1016/j.ijbiomac.2019.03.192 ◽

2019 ◽

Vol 132 ◽

pp. 87-96 ◽

Cited By ~ 7

Author(s):

Sirine Ben Slima ◽

Imen Trabelsi ◽

Naourez Ktari ◽

Sana Bardaa ◽

Karim Elkaroui ◽

...

Keyword(s):

Wound Healing ◽

Sorghum Bicolor ◽

Antioxidant Activities ◽

Burn Wound ◽

Healing Effect ◽

Burn Wound Healing ◽

Wound Healing Effect ◽

Laser Burn ◽

Sorghum Bicolor L

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Morphological features of a cold skin wound under the influence of an extract of cryopreserved skin fragments of piglets (experimental study)

Wiadomości Lekarskie ◽

10.36740/wlek202002119 ◽

2020 ◽

Vol 73 (2) ◽

pp. 306-311

Author(s):

Oleksandr O. Vlasov ◽

Gennadiy O. Kovalov ◽

Mykhailo S. Myroshnychenko

Keyword(s):

Wound Healing ◽

Granulation Tissue ◽

Soft Tissues ◽

Skin Wound ◽

Male Rats ◽

Epithelial Layer ◽

Healing Effect ◽

Group I ◽

Wound Healing Effect ◽

Cryopreserved Skin

The aim of the study is to identify in an experiment the effect of an extract of cryopreserved fragments of piglets on the morphological state of a cold skin wound. Materials and methods: Hairless six-month-old male rats were used in the study. They were divided into III groups: group I included 10 rats that had not been manipulated; group II was represented by 10 rats with cold wounds on the lateral surface of the thigh; group III was represented by 10 rats that were with a cold wound, followed by the injection of an extract of cryopreserved skin fragments of piglets into the abdominal cavity at a dose of 50 μg per 100 g of animal body weight (peptide concentration 100 μg/ ml) once a day for 5 days from the time of wound modeling. Animals in groups I-III were withdrawn from the experiment on the 7th, 14th and 21st days. The material for the morphological study was the fragments of intact skin with underlying soft tissues from the thigh area in group I and the fragments of skin with underlying soft tissues from the thigh area directly from the zone of cryoexposure in groups II and III. Histological, histochemical and morphometric methods were used. Microspecimens were studied using an Olympus BX-41 microscope (Japan). Statistical processing was performed using the Statistica 6.0 and Microsoft Excel 2003 software package. Nonparametric methods were used to compare numerical values (Mann-Whitney U-test, Kruskal-Wallis test). The significance of differences between the average values of the indicators was taken at the level of p<0.05. Results: The extract of cryopreserved skin fragments of piglets has an effective wound healing effect compared to the healing processes in a cold wound, which was not subjected to any therapeutic effects. It was manifested in the improved process of cleansing the wound from necrotic tissues that entered the zone of primary necrosis, as evidenced by 1,2 times decrease of the zone of primary necrosis on the 7th, 14th and 21st days; a decrease of the zone of secondary necrosis on the 7th, 14th and 21st days, respectively, – 1.2, 1.3, 1.2 times; growth and maturation of granulation tissue activation, as evidenced by an increase in the thickness of a granulation tissue layer on 7, 14, 21 days, respectively, – 1.9, 1.8, 1.2 times; activation of proliferative processes in the epithelial layer located in the marginal sections of the wound defect or covering the regenerate surface, as evidenced by more pronounced acanthotic growths in the underlying tissue and an increase in the thickness of the epithelial layer on the 7th, 14th and 21st days, respectively, – 2.1, 2.0, 2.2 times. Conclusion: The extract of cryopreserved skin fragments of piglets has an effective wound healing effect and can be recommended for further research in order to study the possibility of its use in clinical practice.

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