scholarly journals Effects of Aluminum, Iron and/or Low pH on Rice Seedlings Grown in Solution Culture

2015 ◽  
Vol 17 (4) ◽  
pp. 702-710 ◽  
Author(s):  
Farhana Jamaludin Alia ◽  
Jusop Shamshuddin ◽  
Che Ishak Fauziah ◽  
Mohd Hanif Ahmad Husni ◽  
Qurban Ali Panhwar
Keyword(s):  
Solution Culture ◽  
Low Ph ◽  
Rice Seedlings ◽  
Aluminum Iron

scholarly journals Evaluation of genotypic variation for growth of rice seedlings under optimized hydroponics medium

2018 ◽  
Vol 78 (3) ◽  
Author(s):  
Renu Pandey ◽  
Sandeep Sharma ◽  
Priyanka Borah ◽  
Mukesh Kumar Meena ◽  
Prem Bindraban
Keyword(s):  
Oryza Sativa L ◽  
Genotypic Variation ◽  
Nitrogen Deficiency ◽  
Distance Matrix ◽  
Solution Culture ◽  
Rice Seedlings ◽  
Euclidean Distance Matrix ◽  
Hydroponic System ◽  
Rice Genotypes ◽  
Squared Euclidean Distance

Although rice (Oryza sativa L.) is a semi-aquatic plant, its growth in solution culture is often problematic. In commonly used rice hydroponics media, plants exhibited mild nitrogen deficiency, leaf tip burn, salt deposition, along with zinc and iron deficiency. Therefore, we aimed to optimize the nutrient media for growing rice plants taking into consideration the nutrient concentration, pH and ratio of NH4 + to NO3 – nitrogen (N) and named it as ‘Pusa Rice Hydroponics’ (PusaRicH). PusaRicH contains higher amounts of macronutrients, significantly lower B, Mn and Cl and higher amounts of Zn than two commonly used hydroponics media, ‘Yoshida’ and ‘Kumura B’. The optimal ratio of NH4 + to NO3 – – N in PusaRicH medium was 0.5 mM NH4 + and 7.0 mM NO3 – with pH 5.0. The PusaRicH medium was validated by growing 100 diverse rice genotypes and it significantly outperformed the widely cited ‘Yoshida’ and ‘Kimura B’. Cluster analysis carried out on the squared Euclidean distance matrix of biomass and leaf area values of genotypes revealed four major clusters in all hydroponics medium. However, only PusaRicH medium resulted in ten genotypes as good performers in comparison to other two widely cited media. Therefore, the optimized PusaRicH medium can be used successfully to grow rice seedlings in hydroponic system which will aid in screening large number of genotypes in breeding and other physiological experiments.

Increased lucerne nodulation in acid soils with Sinorhizobium meliloti and lucerne tolerant to low pH and high aluminium

2018 ◽  
Vol 69 (10) ◽  
pp. 1031 ◽  
Author(s):  
Kathryn Wigley ◽  
Hayley J. Ridgway ◽  
Alan W. Humphries ◽  
Ross A. Ballard ◽  
Derrick J. Moot
Keyword(s):  
Sinorhizobium Meliloti ◽  
Acid Soils ◽  
Solution Culture ◽  
Low Ph ◽  
Hydroponic System ◽  
Commercial Strain ◽  
Matter Production ◽  
Inoculant Strain ◽  
Acidic Conditions ◽  
High Aluminium

Acidic conditions with damaging levels of available aluminium (Al3+) currently limit lucerne (Medicago sativa) production on soils in the New Zealand high country and in large areas of Australia. Increased lucerne nodulation could be achieved by using an Al3+-tolerant strain of Sinorhizobium meliloti to inoculate an Al3+-tolerant lucerne line. The Al3+ tolerance of the current commercial Australasian inoculant strain for lucerne, S. meliloti RRI128, was compared with strain SRDI736, selected in Australia for tolerance to low pH. Four Al3+ levels (0, 2, 4 and 8 µm) were created at pH 5.1 in a hydroponic system. The rhizobial strains were evaluated on SARDI AT7, a lucerne line selected for improved growth and nodulation in acidic solution culture with Al3+, and on Stamina 5, a commercial cultivar commonly grown in Australasia. SARDI AT7 when inoculated with strain SRDI736 produced more nodules per plant (3.6 vs 2.4) and had higher nodulation percentage (>80% vs <50%) at all Al3+ levels than when inoculated with RRI128. The percentage of nodulated Stamina 5 plants after inoculation with the commercial strain was lower than after inoculation with strain SRDI736 (10–16% vs 25–70%) at all Al3+ levels. The potential of S. meliloti strains SRDI736, SRDI672 and RRI128 and rates of lime to increase lucerne nodulation and dry matter production in soils of low pH (<5.5, in water) and high Al3+ (>3 mg kg–1 soil) was also investigated in a pot trial. Lime had a more consistent effect than inoculant strain for increasing nodulation. At 0.5 and 2 t lime ha–1, plants inoculated with strains SRDI672 and SRDI736 had more nodules per plant than plants inoculated with the commercial strain. At 4 t lime ha–1, the number of nodules per plant was highest for all three inoculants, and there were no differences among them. This confirms the importance of lime to increase lucerne nodulation in low-pH, high-Al3+ soils. However, where liming is uneconomic or impractical, the results show that it was possible to select rhizobial strains to increase lucerne nodulation in acidic soils with damaging levels of available Al3+.

Effects of root anatomy and Fe plaque on arsenic uptake by rice seedlings grown in solution culture

2010 ◽  
Vol 158 (8) ◽  
pp. 2589-2595 ◽  
Author(s):  
Dan Deng ◽  
Sheng-Chun Wu ◽  
Fu-Yong Wu ◽  
Hong Deng ◽  
Ming-Hung Wong
Keyword(s):  
Solution Culture ◽  
Root Anatomy ◽  
Rice Seedlings ◽  
Arsenic Uptake ◽  
Fe Plaque

scholarly journals Pengujian Nomor-nomor Harapan Padi Tahan Al dan pH Rendah Hasil Seleksi In Vitro dengan Kultur Hara

2016 ◽  
Vol 4 (1) ◽  
pp. 18 ◽  
Author(s):  
Ragapadmi Purnamaningsih ◽  
Ika Mariska
Keyword(s):  
Root Development ◽  
Root Length ◽  
In Vitro Selection ◽  
Acid Soil ◽  
Screening Method ◽  
Culture Method ◽  
Solution Culture ◽  
Low Ph ◽  
Rapid Screening

<p>Rice productivity in acid soil is very low because of low pH,<br />low availability of N, P, K, Ca, Mg, Mo, toxicity of Al and Mn.<br />Development of Al tolerant varieties could increase rice<br />productivity in acid soil. Somaclonal variation and in vitro<br />selection method can be used to develop new Al tolerance<br />varieties. A rapid screening method is needed to select a<br />large number of new genotypes or new inbred lines in plant<br />breeding, such as solution culture methods to evalu-ate Altolerant<br />rice. This methods was used to know the response<br />to Al in the seedling stage, root development, and pH<br />changing. In this experiment solution culture method was<br />used to evaluate the new genotypes derived from somaclonal<br />variation and in vitro selection methods. These new<br />genotypes have been tested the tolerance characteristic by<br />using AlCl36H2O at 6 concentrations (0, 100, 200, 300, 400,<br />and 500 ppm). Yoshida solution with two Al concentration<br />were used to tested these genotypes. Measurement of Al<br />tolerance was based on root development by using Relative<br />Root Length (RRL), the relativity of root length at 45 ppm and<br />0 ppm. Almost all of the genotypes have RRLs higher than<br />0.7, which means that there was a positive correlation<br />between the in vitro method and solution culture method. In<br />this experiment pH changes were not applicable to measure<br />the tolerance of the rice genotypes to Al and low pH.</p>

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