scholarly journals Pengujian Nomor-nomor Harapan Padi Tahan Al dan pH Rendah Hasil Seleksi In Vitro dengan Kultur Hara

2016 ◽  
Vol 4 (1) ◽  
pp. 18 ◽  
Author(s):  
Ragapadmi Purnamaningsih ◽  
Ika Mariska
Keyword(s):  
Root Development ◽  
Root Length ◽  
In Vitro Selection ◽  
Acid Soil ◽  
Screening Method ◽  
Culture Method ◽  
Solution Culture ◽  
Low Ph ◽  
Rapid Screening

<p>Rice productivity in acid soil is very low because of low pH,<br />low availability of N, P, K, Ca, Mg, Mo, toxicity of Al and Mn.<br />Development of Al tolerant varieties could increase rice<br />productivity in acid soil. Somaclonal variation and in vitro<br />selection method can be used to develop new Al tolerance<br />varieties. A rapid screening method is needed to select a<br />large number of new genotypes or new inbred lines in plant<br />breeding, such as solution culture methods to evalu-ate Altolerant<br />rice. This methods was used to know the response<br />to Al in the seedling stage, root development, and pH<br />changing. In this experiment solution culture method was<br />used to evaluate the new genotypes derived from somaclonal<br />variation and in vitro selection methods. These new<br />genotypes have been tested the tolerance characteristic by<br />using AlCl36H2O at 6 concentrations (0, 100, 200, 300, 400,<br />and 500 ppm). Yoshida solution with two Al concentration<br />were used to tested these genotypes. Measurement of Al<br />tolerance was based on root development by using Relative<br />Root Length (RRL), the relativity of root length at 45 ppm and<br />0 ppm. Almost all of the genotypes have RRLs higher than<br />0.7, which means that there was a positive correlation<br />between the in vitro method and solution culture method. In<br />this experiment pH changes were not applicable to measure<br />the tolerance of the rice genotypes to Al and low pH.</p>

scholarly journals Peningkatan Toleransi Alumunium pada Jeruk Batang Bawah dengan Teknik Seleksi In Vitro Berulang

2016 ◽  
Vol 6 (1) ◽  
pp. 33
Author(s):  
Mia Toruan Kosmiatin ◽  
Rosa Yunita ◽  
Ali Husni
Keyword(s):  
In Vitro Selection ◽  
Acid Soil ◽  
Phase 1 ◽  
Aluminum Tolerance ◽  
Basal Medium ◽  
Culture Technique ◽  
Low Ph ◽  
Embryogenic Calli ◽  
Marginal Soil

<p>Aluminum Tolerance Improvement of Rootstock Citrus<br />through Repeated In Vitro Selection. Mia Kosmiatin,<br />Rosa Yunita, and Ali Husni. National orange productivity<br />was trend to decrease because of pathogen attack and<br />reducing of planting area. One of alternative ways to<br />preserve and increase orange productivity was using<br />marginal soil mainly acid soil. This matter pushed the<br />breeder to prepare tolerant rootstock and stable in the acid<br />soil. In vitro culture technique was effective and efficient<br />methods to produce tolerant and stable rootstock in acid soil<br />through simulation of acid soil with addition of high<br />aluminum and low pH in the medium. By the simulation the<br />selection could be done in cell level, so cell was selected<br />after induction of variation. A rootstock which high<br />compatibility with scion, useful rooting, and aluminum<br />tolerance could be increased orange productivity through<br />acid soil development. The research was conducted in 3<br />phase: (1) induction of embryogenic calli, (2) improvement<br />of genetic variation through mutation, and (3) In vitro<br />selection with AlCl3.6H2O for aluminum and low pH tolerant.<br />Immature embryos of rootstock were use as explant. The<br />result showed that the best embryogenic calli were induced<br />on MS basal medium with MW vitamin + NAA 7,5 mg/l +<br />kinetin 0,5 mg/l. Before selection, 1.000 rad dosage was the<br />most tolerant dosage to growth embryogenic calli. After<br />selection, 2.000 rad dosage was the best dosage to produce<br />shoots which stable tolerant to aluminum. Selected 88<br />mutant shoots were produced after three times selection on<br />the same medium which AlCl3.6H2O added at low pH.</p>

scholarly journals Uji Pendahuluan Genotipe-genotipe Kedelai Hasil Seleksi In Vitro terhadap Cekaman Aluminium dan pH Rendah

2016 ◽  
Vol 1 (2) ◽  
pp. 73
Author(s):  
Arief Vivi Noviati ◽  
Sri Hutami ◽  
Ika Mariska ◽  
Endang Sjamsudin
Keyword(s):  
Yield Components ◽  
Aluminum Toxicity ◽  
Acid Soil ◽  
Acid Soils ◽  
Mutation Breeding ◽  
Low Ph ◽  
Al Tolerance ◽  
Major Constraint ◽  
Gamma Irradiated

<p class="p1">Aluminum toxicity is a major constraint to soybean production in acid soils. Since variabilities on Al tolerance in plants are very limited, mutation breeding, and <em>in vitro </em>selection were used to increase the variability. Three soyben genotypes were produced from cultivars Wilis and Sindoro that have been gamma irradiated and selected <em>in vitro </em>for their tolerance to Al on Al and low pH media. These genotypes and their original cultivars were then planted in a greenhouse in an acid soil on May 2001. The results showed that the plant performances were varied, some were shorter and more compact than the original. Based on the yield components, a number of plants from the genotypes showed higher than those of the control cultivars. These plants were considered more tolerant to Al than the original cultivars.</p>

scholarly journals UJI KETAHANAN TERHADAP ALUMINIUM DAN pH RENDAH PADA JAGUNG (Zea mays L) VARIETAS PIONEER DAN SRIKANDI SECARA IN VITRO

1970 ◽  
Vol 2 (3) ◽  
pp. 292-299
Author(s):  
Sri Romaito Dalimunthe ◽  
Abdullah Bin Arif ◽  
Irpan Badrul Jamal
Keyword(s):  
Acid Soil ◽  
Low Ph ◽  
Leaf Length ◽  
Al Toxicity ◽  
Marginal Land ◽  
Maize Productivity ◽  
Maize Varieties ◽  
Relative Root Length ◽  
Relative Root

One effort to increase maize productivity is by using marginal land for maize plantation. The marginal land that can be used is acid soil, but the problem are Al toxicity and low pH. To cope with these problems, cultivars having tolerance to Al toxicity and low pH are needed. Plant material used in this research were two maize varieties (Pioneer and Srikandi). Media selection is used there are 4 doses treatment aluminium (0, 250, 500 and 750 ppm AlCl3). All variables were observed except at the variable width of the leaf in this results showed that the treatment of aluminium (AlCl3) would not influence. Treatment combination Variety Srikandi and 250 ppm AlCl3 produces plant height, leaf length and leaf width is the shortest compared to other treatments. Variety Pioneer and Srikandi are suspected of varieties resistant aluminum and low pH values based on relative root length.

scholarly journals Phytochemical Profiles, Physicochemical Analysis, and Biological Activity of Three Colchicum Species

2021 ◽  
Vol 16 (2) ◽  
Author(s):  
Ali Davoodi ◽  
Mohammad Azadbakht ◽  
Seyed Jalal Hosseinimehr ◽  
Saeed Emami ◽  
Masoud Azadbakht
Keyword(s):  
Physicochemical Properties ◽  
Large Scale ◽  
Protein Denaturation ◽  
Potassium Dichromate ◽  
Biological Effects ◽  
Screening Method ◽  
Rapid Screening ◽  
Total Phenolic ◽  
Anti Inflammatory

Background: Colchicum is a genus of the Colchicaceae family with various isolated compounds, especially tropolone alkaloids and flavonoids, which are used for osteoarthritis, gout, cancer, inflammatory diseases, jaundice, and sexual impotence in different societies. Objectives: The current study aimed to evaluate the phytochemical and physicochemical properties and anti-inflammatory activities of three Colchicum species. Methods: Total tropolone alkaloid, total phenolic/total tannin, and total flavonoid contents were determined using acidic potassium dichromate, Folin-Ciocalteu, and aluminum chloride methods, respectively. Moreover, the HPLC method was used for identification and quantitation purposes of tropolone alkaloids. Physicochemical properties of three Colchicum species, including macroscopic and organoleptic properties, solubility, foreign matter, ash values, and heavy metal contents, were evaluated. Besides, in vitro anti-inflammatory activities of the corms also were determined using the protein denaturation technique as a rapid screening method. Results: The highest levels of tropolone alkaloid, phenolic compounds, tannins, and flavonoids were observed in C. autumnale, C. speciosum, and C. robustum, respectively. HPLC analysis indicated the presence of colchicine, demecolcine, 2-demethyl colchicine, 3-demethyl colchicine, colchicoside, colchifoline, cornigerine, and N-deacetyl-N-formyl colchicine in these Colchicum species. The physicochemical properties of C. speciosum and C. robustum corms are appropriate compared to the standard Colchicum autumnale corm. Moreover, all Colchicum species exhibited high anti-inflammatory activities compared to standard drugs. Conclusions: This study demonstrated that the corm of the Colchicum species contained similar main compounds with different amounts, as well as appropriate physicochemical properties. Moreover, the valuable biological effects of these plants stimulate the cultivations on a large scale.

scholarly journals Drought adaptive prediction in potato (Solanum tuberosum) using in vitro and in vivo approaches

2021 ◽  
Vol 22 (2) ◽  
Author(s):  
JANE KATHRYNE JOLANDA LAISINA ◽  
AWANG MAHARIJAYA ◽  
SOBIR SOBIR ◽  
AGUS PURWITO
Keyword(s):  
Solanum Tuberosum ◽  
Drought Stress ◽  
Plant Height ◽  
Root Length ◽  
In Vitro Selection ◽  
Fresh Weight ◽  
Adaptive Prediction ◽  
Tuber Fresh Weight

Abstract. Laisina JKJ, Maharijaya A, Sobir, Purwito A. 2021. Drought adaptive prediction in potato (Solanum tuberosum) using in vitro and in vivo approaches. Biodiversitas 22: 537-545. Prediction of drought adaptive potatoes requires selection indicators. This study aimed to determine in vitro selection indicators for obtaining drought adaptive potato genotypes in vitro and in vivo. The in vitro study was performed using single-node explants from seven genotypes cultured for six weeks on MS medium added with 0.2 M sorbitol to achieve the research aims. The cultures were partially observed and followed by watering the in vitro tubing medium. For the in vivo study, drought stress was applied for 21 days when the plants reached 25 days after planting (DAP) and for 14 days when the plants reached 50 DAP, then plants were irrigated again until harvesting. The results showed that plant height and root length can be used as selection indicators because they had a relative decrease below 50%, a close relationship and had the same variance with the in vitro number of tubers, in vitro tuber fresh weight and in vivo tuber fresh weight in drought stress at 25 DAP. These characters were determinant characters for the clustering pattern in the heat map cluster analysis. Based on these characters, SSI analysis was also performed and it was found that the PKHT4 was more adaptive than the PKHT6 in vitro, but the two genotypes were classified in the medium adaptive in vivo. In conclusion, plant height and root length were determined as the in vitro selection indicators in predicting drought adaptive genotypes in vivo at the plant age of 25 DAP.

Comparison between VIDAS Automatic Enzyme-Linked Fluorescent Immunoassay and Culture Method for Salmonella Recovery from Pork Carcass Sponge Samples

2002 ◽  
Vol 65 (10) ◽  
pp. 1656-1659 ◽  
Author(s):  
KUANG-SHENG YEH ◽  
CHIN-EN TSAI ◽  
SHIH-PING CHEN ◽  
CHAO-WEI LIAO
Keyword(s):  
False Negative ◽  
Screening Method ◽  
Culture Method ◽  
Automated System ◽  
Rapid Screening ◽  
Assay Method ◽  
Intensive Culture ◽  
Negative Results ◽  
False Negative Results ◽  
Potential Alternative

VIDAS Salmonella (VIDAS-SLM) is an automated system that uses the enzyme-linked fluorescent assay method to detect Salmonella species. This study evaluated the efficacy of the VIDAS-SLM method in detecting Salmonella species in pork carcass sponge samples gathered from 10 slaughter plants in Taiwan. Two hundred fifty-seven pork carcass sponge samples were screened by the VIDAS-SLM method and by the culture method in parallel. While 18 sponge samples were found to test positive by both methods, the VIDAS-SLM method detected four additional positive samples for which the culture method failed to recover Salmonella. The specificity of the VIDAS-SLM method was found to be 0.98, and its sensitivity was 1.0, since no false-negative results occurred. Artificially inoculated Salmonella at concentrations as low as 5.0 × 100 CFU/ml was detected in the heat-inactivated sponge sample in the presence or absence of 5.0 × 104 CFU of Citrobacter freundii per ml. Thus, the VIDAS-SLM method is a rapid screening method and a potential alternative to the time- and labor-intensive culture method.

Screening wheat genotypes for tolerance of soil acidity

2003 ◽  
Vol 54 (5) ◽  
pp. 445 ◽  
Author(s):  
C. Tang ◽  
M. Nuruzzaman ◽  
Z. Rengel
Keyword(s):  
Root Length ◽  
Growth Parameters ◽  
Acid Soil ◽  
Genotypic Variation ◽  
Screening Method ◽  
Acid Soils ◽  
Wheat Cultivars ◽  
Breeding Lines ◽  
Wheat Genotypes ◽  
Subsoil Acidity

A soil-based screening method was used to test tolerance of wheat genotypes to acidity. Plants were grown for 6 days in an acid soil with the pH adjusted to 3.9–5.8. The number and length of roots were measured. To validate the method, 12 wheat cultivars of known acidity tolerance and one acid-sensitive barley cultivar were grown on an acid soil in the field with or without amelioration of subsoil acidity. The relative yields of these wheat genotypes on the soil with subsoil acidity ranged from 50 to 89% of yields on soil without subsoil acidity, and were correlated with root growth parameters obtained in the glasshouse. The best correlation was obtained between relative yields in the field (y) and root length per plant (x) at pH 3.9 in the glasshouse (y = –43 + 52*log x, r = 0.95) or root length per plant at pH 3.9 as a percentage of root length at pH 4.8 (y = 1.2 + 46*log x, r = 0.94). Following validation of the glasshouse screening method in the field, 115 wheat genotypes, including cultivars and breeding lines, were screened in the glasshouse. A substantial genotypic variation in acidity tolerance was found, with root length per plant at pH 3.9 ranging from 66 to > 350 mm. Many Western Australian breeding lines displayed better tolerance than existing tolerant wheat cultivars. The screening system can be instrumental in breeding wheat for increased tolerance to acid soils.

scholarly journals Seed Treatment with 2,4-Diacetylphloroglucinol-Producing Pseudomonads Improves Crop Health in Low-pH Soils by Altering Patterns of Nutrient Uptake

2009 ◽  
Vol 99 (5) ◽  
pp. 506-511 ◽  
Author(s):  
Rosa E. Raudales ◽  
Erica Stone ◽  
Brian B. McSpadden Gardener
Keyword(s):  
Abiotic Stress ◽  
Nutrient Uptake ◽  
Seed Treatment ◽  
Acid Soil ◽  
Low Ph ◽  
Mineral Nutrient ◽  
Antifungal Metabolites ◽  
Growing Seasons ◽  
Stress Amelioration

Seed treatment with a 2,4-diacetylphloroglucinol (DAPG)-producing Pseudomonas strain ameliorated abiotic stress disorder in corn caused by growth in a low-pH soil. In two consecutive growing seasons, Wood1R-treated seed gave rise to plants that grew taller (P ≤ 0.05), had fewer foliar lesions (P ≤ 0.10), and provided greater yields (P ≤ 0.1) than the negative controls when grown in soil with a pH < 5.0. Under controlled conditions, seed treatment with Wood1R also reduced foliar lesion severity (P ≤ 0.05 in two of three experiments) but failed to increase shoot or root growth in young seedlings grown in acidic soil. Significant (P ≤ 0.05) patterns of altered mineral nutrient uptake (i.e., generally increasing P and Mg while reducing Al) were observed to occur as a result of Wood1R seed treatment under both sets of growing conditions. In contrast, suppression of seedling damping-off disease was not indicated in this low-pH soil, because no difference in crop stand was observed for any experiment. Additionally, Wood1R-mediated growth inhibition of seedling pathogens was reduced in vitro at pH < 5.0, indicating that secretion of antifungal metabolites may not occur in low-pH soils. This is the first report of an abiotic stress amelioration of acid soil stress-related symptoms by a DAPG-producing pseudomonad.

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