Pengaruh Polimorfisme Gen Heat Shock Protein 70 (HSP70) SacII terhadap Toleransi Panas Itik Lokal Sumatera Barat

ABSTRAK. Tujuan dari penelitian ini adalah untuk mengidentifikasi pengaruh polimorfisme gen heat shock protein 70 (HSP70) terhadap sifat toleransi panas. Genotiping dilakukan pada 110 itik lokal dari 4 populasi itik Sumatera Barat (pitalah, bayang, kamang, dan payakumbuh). Enzim restriksi yang digunakan adalah SacII. Cekaman panas dilakukan selama 1 jam dengan sampel itik lokal sebanyak 24 ekor untuk menganalisa status hematologi. PCR-RFLP dan Sanger sequencing assay digunakan untuk mengidentifikasi polimorfisme. Analisis polimorfisme dilakukan dengan menggunakan Software MEGA 7 dan pengaruhnya dianalisis dengan ANOVA menggunakan software SAS 9.4. Produk amplifikasi yang dihasilkan yaitu 466 pasang basa. Lokus HSP70/SacII mengenali satu situs SNP (Single nucleotide Polymorphism) g.1702TC, menghasilkan dua alel (T dan C) dan tiga genotype (TT, TC, CC). Gen HSP70/SacII bersifat polimorfik pada semua populasi. Hasil penelitian menunjukkan bahwa alel T memiliki frekuensi yang lebih tinggi daripada alel C pada semua populasi. Analisis chi-kuadrat (χ2) menunjukkan bahwa semua populasi itik lokal memenuhi kaidah kesetimbangan Hardy-Weinberg. Pengaruh genotipe gen HSP70 terhadap status hematologi menunjukkan perbedaan (P0,05) terhadap nilai heterofil dan nilai H/L dengan genotipe CT menunjukkan toleransi panas yang lebih baik dibanding genotipe lainnya. Gen HSP70/SacII dapat dijadikan marka molekuler sifat toleransi panas pada itik lokal berdasarkan keragaman dan pengaruhnya terhadap status hematologi. (The effect of heat shock protein 70 (HSP70) SacII gene polymorphism on heat tolerance of West Sumatera local duck) ABSTRACT. The objective of this study was to identify the effect of HSP70/SacII gene polymorphism associated with the thermotolerance trait. Genotyping was performed on 110 local duck from the West Sumatera ducks population (pitalah, bayang, kamang, and payakumbuh). The restriction enzyme used was SacII. Heat stress was done for 1 hour with samples used as much as 24 birds to analyze hematological status. PCR-RFLP and Sanger sequencing assays were used to identify polymorphism. Analysis of polymorphism was conduct by MEGA 7 software and its effect is analyzed with ANOVA using Statistical SAS 9.4 software. The product of amplification was 466 bp. HSP70/SacII recognized one SNP g.1702TC, producing two alleles (T and C) and three genotypes (TT,TC,CC). The HSP70/SacII locus were polymorphic in all population. T allele had a higher frequency than C allele in all populations. The analysis of chi-square (χ2) showed that all local ducks population were fit with Hardy-Weinberg equilibrium. The effect of HSP70/SacII gene on hematological status showed differences (P0,05) of heterophil and H/L ratio with CT genotype show better heat tolerance than other genotypes. HSP70/SacII gene can be used as a marker of heat tolerance in local ducks based on polymorphism and its effect on hematological status.

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Identification of Leishmania (Viannia) species and clinical isolates of Leishmania (Leishmania) amazonensis from Brazil using PCR-RFLP of the heat-shock protein 70 gene reveals some unexpected observations

Diagnostic Microbiology and Infectious Disease ◽

10.1016/j.diagmicrobio.2018.03.004 ◽

2018 ◽

Vol 91 (4) ◽

pp. 312-318 ◽

Cited By ~ 14

Author(s):

Caroline R. Espada ◽

Paola A. Ortiz ◽

Jeffrey J. Shaw ◽

Aldina M.P. Barral ◽

Jackson M.L. Costa ◽

...

Keyword(s):

Heat Shock ◽

Heat Shock Protein ◽

Heat Shock Protein 70 ◽

Leishmania Amazonensis ◽

Clinical Isolates ◽

Pcr Rflp

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Study of the heat shock protein 70-1 gene polymorphism and the risk of nephropathy in type II diabetic patients

Menoufia Medical Journal ◽

10.4103/1110-2098.145519 ◽

2014 ◽

Vol 27 (3) ◽

pp. 582

Author(s):

EmanM Abd El Gayed ◽

NaglaaM Ghanayem ◽

MaathirK El-Shafie ◽

EmanA.F. Badr ◽

Elsayed Elnour ◽

...

Keyword(s):

Heat Shock ◽

Gene Polymorphism ◽

Heat Shock Protein ◽

Heat Shock Protein 70 ◽

Diabetic Patients ◽

Type Ii

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A Rapid Method to Study Heat Shock Protein 70-2 Gene Polymorphism in Insulin-Dependent Diabetes Mellitus

Pancreas ◽

10.1097/00006676-199610000-00009 ◽

1996 ◽

Vol 13 (3) ◽

pp. 268-272 ◽

Cited By ~ 8

Author(s):

Lee-Ming Chuang ◽

Tzuu-Shuh Jou ◽

Huey-Peir Wu ◽

Tong-Yuan Tai ◽

Boniface J. Lin

Keyword(s):

Diabetes Mellitus ◽

Heat Shock ◽

Gene Polymorphism ◽

Heat Shock Protein ◽

Rapid Method ◽

Heat Shock Protein 70 ◽

Insulin Dependent Diabetes Mellitus ◽

Insulin Dependent Diabetes ◽

Dependent Diabetes Mellitus ◽

Insulin Dependent

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Heat-shock protein 70 PCR-RFLP: a universal simple tool for Leishmania species discrimination in the New and Old World

Parasitology ◽

10.1017/s0031182010000089 ◽

2010 ◽

Vol 137 (8) ◽

pp. 1159-1168 ◽

Cited By ~ 75

Author(s):

A. M. MONTALVO ◽

J. FRAGA ◽

L. MONZOTE ◽

I. MONTANO ◽

S. DE DONCKER ◽

...

Keyword(s):

Heat Shock ◽

Heat Shock Protein ◽

Restriction Fragment ◽

Heat Shock Protein 70 ◽

New World ◽

Phylogenetic Study ◽

Species Discrimination ◽

Pcr Rflp ◽

Specific Restriction ◽

Species Specific

SUMMARYIntroduction. Species typing in leishmaniasis gains importance in diagnostics, epidemiology, and clinical studies. A restriction fragment length polymorphism (RFLP) assay of PCR amplicons from a partial heat-shock protein 70 gene (hsp70) had been described for the New World, allowing identification of some species. Methods. Based on an initial in silico analysis of 51 hsp70 sequences, most of which we recently determined in the frame of a phylogenetic study, species-specific restriction sites were identified. These were tested by PCR-RFLP on 139 strains from 14 species, thereby documenting both inter- and intra-species variability. Results. Our assay could identify Leishmania infantum, L. donovani, L. tropica, L. aethiopica, L. major, L. lainsoni, L. naiffi, L. braziliensis, L. peruviana, L. guyanensis, and L. panamensis by applying 2 subsequent digests. L. mexicana, L. amazonensis, and L. garnhami did not generate species-specific restriction fragment patterns. Conclusion. Currently no assay is available for global Leishmania species discrimination. We present a universal PCR-RFLP method allowing identification of most medically relevant Old and New World Leishmania species on the basis of a single PCR, obviating the need to perform separate PCRs. The technique is simple to perform and can be implemented in all settings where PCR is available.

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Expression of substance P, vasoactive intestinal peptide and heat shock protein 70 in nasal mucosal smears of patients with allergic rhinitis: investigation using a liquid-based method

The Journal of Laryngology & Otology ◽

10.1017/s0022215107001454 ◽

2008 ◽

Vol 122 (7) ◽

pp. 700-706 ◽

Cited By ~ 11

Author(s):

T Chalastras ◽

P Nicolopoulou-Stamati ◽

E Patsouris ◽

A Eleftheriadou ◽

D Kandiloros ◽

...

Keyword(s):

Allergic Rhinitis ◽

Heat Shock ◽

Substance P ◽

Heat Shock Protein ◽

Vasoactive Intestinal Peptide ◽

Positive Reaction ◽

Nasal Mucosa ◽

Heat Shock Protein 70 ◽

Seasonal Allergic Rhinitis ◽

Chi Square

AbstractObjective:The aim of this study was to investigate expression of the neuropeptides substance P, vasoactive intestinal peptide and heat shock protein 70 in the nasal mucosa cells of patients with seasonal allergic rhinitis, in order to obtain more information on the pathophysiological and immunological role of these markers in allergic rhinitis.Material and methods:Nasal epithelium specimens obtained from 42 patients with allergic rhinitis were studied, using Shandon's Papspin liquid-based cytology method. Smears were immunostained with antibodies against substance P, vasoactive intestinal peptide and heat shock protein 70, and the results were correlated with the clinical features of seasonal allergic rhinitis.Results:A positive reaction for substance P, vasoactive intestinal peptide and heat shock protein 70 was observed in 73.8, 66.7 and 69.0 per cent of the allergic rhinitis mucosal smears, respectively. The Pearson chi-square test showed that 40.5 per cent of the immunostained smears had a positive reaction for one or two of the markers studied (i.e. substance P, vasoactive intestinal peptide or heat shock protein 70), and that 47.6 per cent of the smears had a positive reaction for all the markers (p < 0.0001).Conclusions:We found a high level of expression of substance P and vasoactive intestinal peptide in the nasal mucosa smears of patients suffering from allergic rhinitis. This indicates a role for these neuropeptides in the neuroregulation of immunity and hypersensivity in this disease. Furthermore, expression of heat shock protein 70 may contribute to the development of allergic rhinitis.

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