scholarly journals Prognostic value of the synovial fluid peroxidation indicators to determine the risk of implant instability in patients with gonarthrosis accompanied by articular surface defects

2017 ◽  
Vol 23 (4) ◽  
pp. 455-459
Author(s):  
D.Iu. Borzunov ◽  
◽  
E.L. Matveeva ◽  
E.S. Spirkina ◽  
O.K. Chegurov ◽  
...  
Keyword(s):  
Synovial Fluid ◽  
Prognostic Value ◽  
Surface Defects ◽  
Articular Surface

scholarly journals State of Synovial Medium in Patients with Degenerative Dystrophic Changes of Knee Joint Accompanied by Articular Surface Defects

2016 ◽  
Vol 23 (4) ◽  
pp. 40-43
Author(s):  
E. L Matveeva ◽  
A. G Gasanova ◽  
O. K Chegurov
Keyword(s):  
Synovial Fluid ◽  
Knee Joint ◽  
Subchondral Bone ◽  
Mineral Content ◽  
Surface Defects ◽  
Articular Surface ◽  
Calcium Content ◽  
Mineral Elements ◽  
Phosphate Ions

The purpose of the work was to determine the concentrations of mineral elements in subchondral bone and synovial fluid of patients with degenerative dystrophic knee joint diseases accompanied by articular surface defects. Subchondral bone and synovial fluid were used as a study material. Analysis of mineral content indices included the determination of calcium, phosphate, magnesium and chlorides concentrations by spectrophotometry. It was shown that the indices of basic electrolytes, i.e. of calcium and phosphate ions, in bone and synovial fluid did not correlate with each other and changed in different directions. Calcium content decreased in the bone while phosphate ions - in synovial fluid.

scholarly journals State of Synovial Medium in Patients with Degenerative Dystrophic Changes of Knee Joint Accompanied by Articular Surface Defects

2016 ◽  
pp. 40-43
Author(s):  
E. L. Matveeva ◽  
A. G. Gasanova ◽  
O. K. Chegurov
Keyword(s):  
Synovial Fluid ◽  
Knee Joint ◽  
Subchondral Bone ◽  
Mineral Content ◽  
Surface Defects ◽  
Articular Surface ◽  
Calcium Content ◽  
Mineral Elements ◽  
Phosphate Ions

The purpose of the work was to determine the concentrations of mineral elements in subchondral bone and synovial fluid of patients with degenerative dystrophic knee joint diseases accompanied by articular surface defects. Subchondral bone and synovial fluid were used as a study material. Analysis of mineral content indices included the determination of calcium, phosphate, magnesium and chlorides concentrations by spectrophotometry. It was shown that the indices of basic electrolytes, i.e. of calcium and phosphate ions, in bone and synovial fluid did not correlate with each other and changed in different directions. Calcium content decreased in the bone while phosphate ions - in synovial fluid.

The lubricating ability of biomembrane models with dipalmitoyl phosphatidylcholine and γ-globulin

1998 ◽  
Vol 212 (5) ◽  
pp. 337-346 ◽  
Author(s):  
H Higaki ◽  
T Murakami ◽  
Y Nakanishi ◽  
H Miura ◽  
T Mawatari ◽  
...  
Keyword(s):  
Friction Coefficient ◽  
Synovial Fluid ◽  
Articular Surface ◽  
Glass Plate ◽  
Sodium Hyaluronate ◽  
Load Condition ◽  
Dipalmitoyl Phosphatidylcholine ◽  
Adsorbed Films ◽  
Articular Surfaces ◽  
Frictional Behaviour

Two kinds of friction tests were conducted to investigate the lubricating effect of the injection of amphiphilies on the osteoarthritic joint. The effects of the addition of Lα-dipalmitoyl phosphatidylcholine (Lα-DPPC) riposomes and γ-globulin in a saline solution of sodium hyaluronate (HA) were evaluated through pendulum friction tests. The frictional characteristics of pig shoulder joints were confirmed to depend on the viscosity of the lubricants only in the physiologically low load condition and in the condition immediately after loading. Detergent (polyoxyethylene p-t-octylphenyl ether) was successfully used to remove adsorbed films from the articular surfaces. The friction coefficient of natural synovial joints was significantly increased in a mode of mixed lubrication with the HA solution of 0.2 g/dl by the treatment of the surface with the detergent. The addition of Lα-DPPC riposomes or y-globulin significantly improved the boundary lubricating ability of the articular surfaces treated with the detergent, depending on the quantity of those additives. It appears that the Lα-DPPC riposomes and γ-globulin can form protective films on the articular surfaces like a biomembrane. Moreover, the reciprocating frictional behaviour in sliding pairs of pig articular cartilages and glass plates was studied in order to elucidate the tribological role of those constituents in the boundary lubricating film on the articular surface. Pig synovial fluid and water solutions of HA were used as lubricants. The synovial fluid had superior lubricating ability compared to the HA solution of equivalent viscosity under a physiologically high load condition. This fact seems to be responsible for the boundary lubricating ability of constituents other than hyaluronic acid. Langmuir-Blodgett (LB) films of Lα-DPPC on the glass plate were kept at a low and stable friction coefficient, depending on the number of film layers. In conditions of mixed films with Lα-DPPC and γ-globulin, the frictional behaviour was improved by increasing the quantity of γ-globulin. A model is proposed in which the effective adsorbed films are composed of proteins, phospholipids and other conjugated constituents on the articular surfaces to be accurate in describing the boundary lubricating mechanism. The mechanism is controlled by hydrophobic groups in those amphiphilies.

Wear of Articular Cartilage: The Effect of Crystals

1993 ◽  
Vol 207 (1) ◽  
pp. 41-58 ◽  
Author(s):  
A Hayes ◽  
B Harris ◽  
P A Dieppe ◽  
S E Clift
Keyword(s):  
Articular Cartilage ◽  
Synovial Fluid ◽  
Crystal Size ◽  
Articular Surface ◽  
Normal Cartilage ◽  
Pin On Disc ◽  
Size And Morphology ◽  
Arthritic Joints

An investigation of the effect of crystals in a lubricant on the wear of articular cartilage in vitro was carried out in order to examine the hypothesis that crystals present in synovial fluid could cause abrasive damage of the articular surface. Plugs of cartilage were worn against a stainless steel counterface in a pin-on-disc wear rig. The concentration of cartilage debris present in the lubricant was assessed by measuring the bound sulphate originating from the glycosaminoglycans by ion chromatography. Results indicated that the presence of crystals in the lubricant significantly increased the concentration of wear debris and that the crystal size and morphology influenced the type of damage sustained by the cartilage. Other experimental evidence suggested that cartilage scratched in vivo was no more susceptible to further in vitro damage in this experimental model than normal cartilage. These results implied that crystals present in the synovial fluid of arthritic joints have the potential to cause excessive wear of the articular surface, but that if such crystals are removed the scratched cartilage may not be susceptible to any further damage by abrasive wear.

scholarly journals Induction of Host Matrix Metalloproteinases by Borrelia burgdorferi Differs in Human and Murine Lyme Arthritis

2005 ◽  
Vol 73 (1) ◽  
pp. 126-134 ◽  
Author(s):  
Aruna K. Behera ◽  
Ethan Hildebrand ◽  
Joanna Scagliotti ◽  
Allen C. Steere ◽  
Linden T. Hu
Keyword(s):  
Synovial Fluid ◽  
Matrix Metalloproteinases ◽  
Borrelia Burgdorferi ◽  
Articular Surface ◽  
Expression Patterns ◽  
Gene Array ◽  
Lyme Arthritis ◽  
Cartilage Tissue ◽  
Enzyme Linked Immunosorbent Assay ◽  
Tissue Inhibitor

ABSTRACT Matrix metalloproteinases (MMPs) are induced from host tissues in response to Borrelia burgdorferi. Upregulation of MMPs may play a role in the dissemination of the organism through extracellular matrix tissues, but it can also result in destructive pathology. Although mice are a well-accepted model for Lyme arthritis, there are significant differences compared to human disease. We sought to determine whether MMP expression could account for some of these differences. MMP expression patterns following B. burgdorferi infection were analyzed in primary human chondrocytes, synovial fluid samples from patients with Lyme arthritis, and cartilage tissue from Lyme arthritis-susceptible and -resistant mice by using a gene array, real-time PCR, an enzyme-linked immunosorbent assay, and immunohistochemistry. B. burgdorferi infection significantly induced transcription of MMP-1, -3, -13, and -19 from primary human chondrocyte cells. Transcription of MMP-10 and tissue inhibitor of metalloprotease 1 was increased with B. burgdorferi infection, but protein expression was only minimally increased. The synovial fluid levels of MMPs from patients with high and low spirochete burdens were consistent with results seen in the in vitro studies. B. burgdorferi-susceptible C3H/HeN mice infected with B. burgdorferi showed induction of MMP-3 and MMP-19 but no other MMP or tissue inhibitor of metalloprotease. As determined by immunohistochemistry, MMP-3 expression was increased only in chondrocytes near the articular surface. The levels of MMPs were significantly lower in the more Lyme arthritis-resistant BALB/c and C57BL/6 mice. Differences between human and murine Lyme arthritis may be related to the lack of induction of collagenases, such MMP-1 and MMP-13, in mouse joints.

Osteochondral graft from the pre-achilles area for replacement of articular surface defects

2017 ◽  
Author(s):  
V. V. Kuznetsov ◽  
I. A. Pahomov ◽  
V. M. Prohorenko ◽  
M. A. Sadovoy ◽  
A. V. Korel ◽  
...  
Keyword(s):  
Surface Defects ◽  
Articular Surface ◽  
Osteochondral Graft

A Mechanism to Explain Physiological Lubrication

2010 ◽  
Vol 132 (7) ◽  
Author(s):  
David F. James ◽  
Garret M. Fick ◽  
W. Douglas Baines
Keyword(s):  
Hyaluronic Acid ◽  
Synovial Fluid ◽  
Articular Surface ◽  
Constant Load ◽  
Squeeze Film ◽  
Hydrodynamic Resistance ◽  
Low Viscosity ◽  
Electrostatic Charges ◽  
Articular Surfaces

A new mechanism of physiological lubrication is proposed to explain how low-viscosity synovial fluid prevents articular surfaces from contacting and wearing. The new mechanism is based on the hypothesis that the hyaluronic acid chains in synovial fluid bind to the cartilage surfaces through electrostatic charges, with the phospholipid layer on an articular surface supplying the necessary attractive charges. The stationary hyaluronic acid network causes a large hydrodynamic resistance to outward flow from the gap. To determine the effectiveness of the network in preventing contact, squeeze-film flow between two incompressible, permeable disks is analyzed when a constant load is suddenly applied, and the solvent—synovial fluid minus the hyaluronic acid—escapes through the network and through the permeable disks. The analysis yields the approximate time for the gap distance to decrease to asperity size. For realistic physiological parameters, the time for the surfaces to contact is a minimum of several minutes and likely much longer. The role of albumin in the synovial fluid is included because the large protein molecules are trapped by the small openings in the hyaluronic acid network, which increases the flow resistance of the network and thereby delays contact of the surfaces.

scholarly journals Osteochondral graft from the pre-Achilles area for repair of ankle joint articular surface defects and lesions

2017 ◽  
Vol 23 (4) ◽  
pp. 405-410
Author(s):  
V.V. Kuznetsov ◽  
◽  
I.A. Pakhomov ◽  
A.M. Zaidman ◽  
A.V. Korel' ◽  
...  
Keyword(s):  
Ankle Joint ◽  
Surface Defects ◽  
Articular Surface ◽  
Osteochondral Graft

scholarly journals Core-2 O-glycans are required for galectin-3 interaction with the osteoarthritis related protein lubricin

2019 ◽  
Author(s):  
Sarah A. Flowers ◽  
Kristina A. Thomsson ◽  
Liaqat Ali ◽  
Shan Huang ◽  
Yolanda Mthembu ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Synovial Fluid ◽  
Surface Plasmon ◽  
Binding Capacity ◽  
Articular Surface ◽  
Cross Linking ◽  
Related Protein ◽  
Proteoglycan 4 ◽  
Galectin 3 ◽  
Surface Active

ABSTRACTSynovial fluid lubricin (proteoglycan 4) is a mucin-type O-linked glycosylated (60% of the mass) biological lubricant involved in osteoarthritis (OA) development. Lubricin has been reported to be cross-linked by synovial galectin-3 on the lubricating articular surface. Here, we confirm that binding to galectin-3 depended on core-2 O-linked glycans, where surface plasmon resonance of a recombinant lubricin (rhPRG4) devoid of core-2 structures lacked binding capacity to recombinant galectin-3. Both galectin-3 levels and interactions with synovial lubricin were found to be decreased in late-stage OA patients coinciding with an increase of truncated and less sialylated core 1 O-glycans. These data suggest a defect cross-linking of surface active molecules in OA and provides novel insights into OA molecular pathology.

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