scholarly journals State of Synovial Medium in Patients with Degenerative Dystrophic Changes of Knee Joint Accompanied by Articular Surface Defects

2016 ◽  
Vol 23 (4) ◽  
pp. 40-43
Author(s):  
E. L Matveeva ◽  
A. G Gasanova ◽  
O. K Chegurov

The purpose of the work was to determine the concentrations of mineral elements in subchondral bone and synovial fluid of patients with degenerative dystrophic knee joint diseases accompanied by articular surface defects. Subchondral bone and synovial fluid were used as a study material. Analysis of mineral content indices included the determination of calcium, phosphate, magnesium and chlorides concentrations by spectrophotometry. It was shown that the indices of basic electrolytes, i.e. of calcium and phosphate ions, in bone and synovial fluid did not correlate with each other and changed in different directions. Calcium content decreased in the bone while phosphate ions - in synovial fluid.

Author(s):  
E. L. Matveeva ◽  
A. G. Gasanova ◽  
O. K. Chegurov

The purpose of the work was to determine the concentrations of mineral elements in subchondral bone and synovial fluid of patients with degenerative dystrophic knee joint diseases accompanied by articular surface defects. Subchondral bone and synovial fluid were used as a study material. Analysis of mineral content indices included the determination of calcium, phosphate, magnesium and chlorides concentrations by spectrophotometry. It was shown that the indices of basic electrolytes, i.e. of calcium and phosphate ions, in bone and synovial fluid did not correlate with each other and changed in different directions. Calcium content decreased in the bone while phosphate ions - in synovial fluid.


2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Mikel Sánchez ◽  
Diego Delgado ◽  
Pello Sánchez ◽  
Emma Muiños-López ◽  
Bruno Paiva ◽  
...  

The aim of this study was to assess a novel approach to treating severe knee osteoarthritis by targeting synovial membrane, superficial articular cartilage, synovial fluid, and subchondral bone by combining intra-articular injections and intraosseous infiltrations of platelet rich plasma. We explored a new strategy consisting of intraosseous infiltrations of platelet rich plasma into the subchondral bone in combination with the conventional intra-articular injection in order to tackle several knee joint tissues simultaneously. We assessed the clinical outcomes through osteoarthritis outcome score (KOOS) and the inflammatory response by quantifying mesenchymal stem cells in synovial fluid. There was a significant pain reduction in the KOOS from baseline(61.55±14.11)to week 24(74.60±19.19), after treatment(p=0.008), in the secondary outcomes (symptoms,p=0.004; ADL,p=0.022; sport/rec.,p=0.017; QOL,p=0.012), as well as VAS score (p<0.001) and Lequesne Index(p=0.008). The presence of mesenchymal stem cells in synovial fluid and colony-forming cells one week after treatment decreased substantially from7.98±8.21 MSC/μL to4.04±5.36 MSC/μL(p=0.019)and from601.75±312.30to139.19±123.61  (p=0.012), respectively. Intra-articular injections combined with intraosseous infiltrations of platelet rich plasma reduce pain and mesenchymal stem cells in synovial fluid, besides significantly improving knee joint function in patients with severe knee osteoarthritis. This trial is registered on EudraCT with the number2013-003982-32.


Author(s):  
SANDRIANI DARINE CALDEIRA ◽  
PRISCILA AIKO HIANE ◽  
MARIA ISABEL LIMA RAMOS ◽  
MANOEL MENDES RAMOS FILHO

Realizou-se a determinação de nutrientes calóricos e oligoelementos macro e microminerais em dois frutos nativos do Estado de Mato Grosso do Sul (MS), o araçá ( Psidium guineense SW.) e o tarumã ( Vitex cymosa Bert). Os dados obtidos mostraram que tanto o araçá quanto o tarumã, pelo alto teor de umidade, apresentam baixo valor calórico (respectivamente, 44,5 e 39,6k cal/ 100 g). Foi verificado em ambos os frutos valores (g/ 100 g de amostra integral) de glicídios redutores e nãoredutores (respectivamente, 7,8 e 9,3) mais elevados do que os dos demais nutrientes energéticos encontrados. Em relação aos teores de minerais, o araçá apresentou maior quantidade de cálcio (26,8 mg/ 100 g), magnésio (17,9 mg/100 g), manganês (0,3 mg/ 100 g) e cobre (0,12 mg/100 g), comparativamente ao fruto tarumã. Entretanto o tarumã apresentou teores mais elevados de fósforo (21,1 mg/100 g), potássio (287,8 mg/100 g) e ferro (0,43 mg/100 g) do que o araçá. Os frutos estudados podem ser considerados, de maneira geral, boas fontes de minerais quando comparados com frutos mais comumente consumidos pela população, como a maçã, a pêra e o abacaxi. PHYSICAL-CHEMICAL CHARACTERIZATION OF ARAÇA (Psidium guineense SW.) AND TARUMÃ (Vitex cymosa Bert.) OF MATO GROSSO DO SUL STATE (BRAZIL) Abstract The determination of caloric nutrients, oligoelements and macro and microminerals in two native fruits of Mato Grosso do Sul state, the araça (Psidium guineense SW.) and the tarumã (Vitex cymosa Bert.) was realized. The obtained data showed that even for araça and for tarumã, by their high moisture content, they showed low caloric value (respectively 44.5 and 39.6 Kcal/100 g). In both fruits it was verified reducing and non-reducing sugars (g/100g sample), being respectively 7.8 and 9.3 for araça and tarumã, showing higher values than the other energetic nutrients found. In relation to the mineral content, the araça showed greater calcium content (26.8 mg/100 g), magnesium (17.9 mg/100 g), manganese (0.3 mg/100 g) and copper (0.12 mg/100 g), comparatively to tarumã fruit. Although, the tarumã showed greater content of phosphorus (21.1 mg/100 g), potassium (287.8 mg/ 100 g) and iron (0.43 mg/100 g), in comparison to the araça. The studied fruits can be considered, in a general way, good sources of minerals when compared to fruits more commonly consumed by the population as apple, pear and pineapple.


2021 ◽  
Vol 64 (3) ◽  
pp. 256-262
Author(s):  
Anila Bashir ◽  
Muhammad Sajid ◽  
Farrukh Siyar Hamid ◽  
Abdul Waheed ◽  
Madiha Bashir ◽  
...  

Camellia sinensis L. leaves composed of different concentrations of mineral contents play a vital role in human nutrition and health. In this study, locally processed three different green tea varieties i.e. P3, P5 and P9 were used for mineral content determination at National Tea and High Value Crops Research Institute (NTHRI), Shinkiari, Mansehra, Pakistan. Atomic absorption spectrophotometer, flame photometer and Kjehldal apparatus were used for the determination of mineral concentration in all the collected tea samples. Mineral composition of tea samples were identified in the following quantity order: high level of nitrogen (37300 to 41380 mg/L), calcium (515.6 to 522.1 mg/L) and phosphorus (742 to 1220 mg/L) were observed in all tea samples compared to other minerals. Cobalt (Co), molybdenum (Mo), sodium (Na), zinc (Zn), nickel (Ni) and copper (Cu) were highest in P3, while least amount was identified in P5, variety. On the contrary, calcium (Ca), potassium (K) and lead (Pb) contents were maximum in P5, while minimum were in P3. This study revealed that the levels of mineral elements in different varieties of green tea vary from the permissible level but the monitoring of their levels in tea plant is obligatory for future risk measurements.  


2004 ◽  
Vol 126 (4) ◽  
pp. 475-484 ◽  
Author(s):  
J. S. Binette ◽  
M. Garon ◽  
P. Savard ◽  
M. D. McKee ◽  
M. D. Buschmann

A tetrapolar method to measure electrical conductivity of cartilage and bone, and to estimate the thickness of articular cartilage attached to bone, was developed. We determined the electrical conductivity of humeral head bovine articular cartilage and subchondral bone from a 1- to 2-year-old steer to be 1.14±0.11S/m(mean±sd,n=11) and 0.306±0.034S/m,(mean±sd,n=3), respectively. For a 4-year-old cow, articular cartilage and subchondral bone electrical conductivity were 0.88±0.08S/m(mean±sd,n=9) and 0.179±0.046S/m(mean±sd,n=3), respectively. Measurements on slices of cartilage taken from different distances from the articular surface of the steer did not reveal significant depth-dependence of electrical conductivity. We were able to estimate the thickness of articular cartilage with reasonable precision (<20% error) by injecting current from multiple electrode pairs with different inter-electrode distances. Requirements for the precision of this method to measure cartilage thickness include the presence of a distinct layer of calcified cartilage or bone with a much lower electrical conductivity than that of uncalcified articular cartilage, and the use of inter-electrode distances of the current injecting electrodes that are on the order of the cartilage thickness. These or similar methods present an attractive approach to the non-destructive determination of cartilage thickness, a parameter that is required in order to estimate functional properties of cartilage attached to bone, and evaluate the need for therapeutic interventions in arthritis.


1999 ◽  
Vol 12 (03) ◽  
pp. 151-155 ◽  
Author(s):  
L. W. Valentino ◽  
E. M. Gaughan ◽  
D. R. Biller ◽  
R. H. Raub ◽  
J. D. Lillich

The purpose of the study is to document the prevalence of articular surface osteochondrosis lesions in feral horses. Eighty yearling feral horses were used. Radiographic images of the left stifle, both tarsocrural, metatarsophalangeal, metacarpophalangeal joints were taken. Radiographs were examined for the presence of osteochondral fragmentation and abnormal outline of subchondral bone suggestive of osteochondrosis. The prevalence of each lesion was calculated for each joint as well as for overall prevalence within the group, the latter being 6.25%. Typical osteochondrosis lesions were found within the tarsocrural and metatarsophalangeal joints. Based on the difference in prevalence of osteochondrosis between feral and certain domestic horses, management practices and perhaps genetic base may have a greater influence on the development of the disease in horses than trauma alone.


1961 ◽  
Vol 36 (2) ◽  
pp. 197-211 ◽  
Author(s):  
lb Lorenzen

ABSTRACT Biochemical and histological changes in the aortic wall of rabbits were demonstrated following injection of epinephrine and l-thyroxine during 2 weeks. The widespread gross and microscopic changes were accompanied by an increase in hexosamine content and uptake of 35S labeled sodium sulphate, and an increased calcium content, whereas the collagen content, assessed by determination of hydroxyproline, was reduced. Comparison with the effect of epinephrine injections alone showed that thyroxine intensified the damaging effect of epinephrine on the vessel wall and induced more pronounced mucopolysaccharide changes in the aortic wall, presumably acting as a link in the healing processes.


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