scholarly journals Induced Mutagenesis in Cicer arietinum

2014 ◽  
Vol 12 ◽  
pp. 1-4 ◽  
Author(s):  
L. Mullainathan ◽  
S. Umavathi
Keyword(s):  
Cicer Arietinum ◽  
Gamma Rays ◽  
Crop Improvement ◽  
Ethyl Methane Sulphonate ◽  
Chick Pea ◽  
Biological Damage ◽  
Methane Sulphonate ◽  
Dose Concentration ◽  
Induced Mutagenesis ◽  
Effectiveness And Efficiency

The traditional varieties of chick pea have low potentiality and restricted variability with respect to economic characters. Broadening the genetic base for crop improvement can be quickly achieved through induced mutagenesis. The present study was undertaken in order to comparing the effectiveness and efficiency of mutagens on Cicer arietinum. In this regard, Co – 4 variety of chick pea was subjected to different dose/concentration of Gamma rays (20, 30, 40, 50 and 60 kR) and Ethyl Methane Sulphonate (10, 20, 30, 40 and 50 mM) for inducing mutation. Mutagenic effectiveness and efficiency was calculated based on biological damage in M1 and chlorophyll mutations in M2. The results indicated that, mutagenic effectiveness increased with the increase in dose/concentration of mutagen. Intermediate treatments in general were found more efficient in causing less biological damage and inducing maximum amount of mutations. It shows that the chemical mutagens are more effective and efficient than physical mutagen for inducing mutation in Chick pea

scholarly journals Induced mutagenesis in Chickpea (Cicer arietinum (L.) with special reference to the frequency and spectrum of chlorophyll mutations

2016 ◽  
Vol 1 (1) ◽  
pp. 49 ◽  
Author(s):  
S. Umavathi ◽  
L. Mullainathan
Keyword(s):  
Cicer Arietinum ◽  
Gamma Rays ◽  
Ethyl Methane Sulphonate ◽  
Ethyl Methane ◽  
Cicer Arietinum L ◽  
Chlorophyll Mutations ◽  
Methane Sulphonate ◽  
Chlorophyll Mutants ◽  
Induced Mutagenesis ◽  
Different Doses

A relative study of frequency and spectrum of chlorophyll mutations induced by mutagens in M2 generation was made with chickpea (Cicer arietinum (L). Variety ‘CO-4’. The treatments include different doses/concentrations of Gamma rays (20, 30, 40, 50 and 60kR) and Ethyl Methane Sulphonate (10, 20, 30, 40 and 50 mM). From the study, the overall frequencies and spectrum of five types of induced chlorophyll mutants Viridis (0.55), Xantha (0.46), Chlorina (0.45), Albina (0.43) and Tigrina (0.35) were observed. The frequency of chlorophyll mutation was increased with increasing concentrations up to a level, beyond it declined in both the mutagens. And the chlorophyll frequency was found in the order of viridis > xantha >chlorina> Albina >tigrina. The chemical mutagen, EMS was found to be more effective in inducing chlorophyll mutations than gamma rays in Chick pea.

scholarly journals Studies on Mutagenic Effectiveness and Efficiency of Gamma Rays and Ethyl Methane Sulphonate in Jasmine

2019 ◽  
pp. 1-8
Author(s):  
Sanchita Ghosh ◽  
M. Ganga ◽  
K. Soorianathasundaram
Keyword(s):  
Gamma Rays ◽  
High Frequency ◽  
Wide Spectrum ◽  
Ethyl Methane Sulphonate ◽  
Biological Damage ◽  
Flower Yield ◽  
Methane Sulphonate ◽  
Chlorophyll Mutants ◽  
Effectiveness And Efficiency ◽  
Morphological Mutation

Background/Aim: The present investigation was undertaken to study the mutagenic effectiveness and efficiency in M1V1 generation and to study effect of gamma rays on spectrum of morphological mutation in Jasminum grandiflorum Linn. cv. White Pitchi. Methods: Terminal cuttings were treated with four doses of gamma rays viz., 10, 15, 20 and 25 Gy and four doses of EMS viz., 25, 30, 35 and 40 mM separately. Both mutagens created a high frequency as well as a wide spectrum of mutation. Results: Totally five types of chlorophyll mutants viz., xantha, viridis, yellow viridis, variegata and tigrina were observed. The mutagenic effectiveness and efficiency were calculated based on biological damage as well as chlorophyll mutation frequency on M1 plants. The mutagenic treatments were effective in inducing various types of morphological macro mutants, with few of them showing significant changes in plant height, flowering parameters and flower yield. The lower mutagen doses were associated with higher mutagenic effectiveness and efficiency. Conclusion: The present study indicated that the physical mutagen gamma rays were more effective and efficient in causing mutations as compared to the chemical mutagen EMS.

scholarly journals Mutagenic effectiveness and efficiency of gamma rays and sodium azide in M2 generation of Cowpea [Vigna unguiculata (L.) Walp.]

2020 ◽  
Author(s):  
Aamir Raina ◽  
Samiullah Khan
Keyword(s):  
Genetic Variability ◽  
Gamma Rays ◽  
Sodium Azide ◽  
Crop Improvement ◽  
Arable Land ◽  
Grain Legume ◽  
Nutrition Security ◽  
Cowpea Varieties ◽  
Induced Mutagenesis ◽  
Effectiveness And Efficiency

AbstractLegumes play a pivotal role in combating the chronic hunger and malnutrition in the developing nations and are also ideal crops to achieve global food and nutrition security. In the era of climate change, erratic rainfalls, depleting arable land and water resource, feeding the rapidly growing population is a challenging task. Among breeding programs for crop improvement, induced mutagenesis has proven to be the viable, effective, robust and coherent tool to facilitate the process of creating varieties with improved yield. Like most other pulses, cowpea is a self pollinated, nutritious, versatile and widely adapted grain legume, but harbour a little accessible genetic variability. Lack of sufficient genetic variability and small size of flowers, traditional plant breeding methods are not enough to meet the demands of improvement of cowpea. Hence, induced mutagenesis was employed to induce significant genetic variability across a range of agro-economical traits in two cowpea varieties Gomati VU-89 and Pusa-578 from M1 to M4 generations. The success of induced mutagenesis largely depends on the selection of appropriate mutagen, its dose, effectiveness and efficiency. Hence present study was conduct to assess the effectiveness and efficiency of single and combined doses of sodium azide and gamma rays to set an appropriate protocol for induced mutagenesis experimentation in cowpea.

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