scholarly journals Ploidia levels in pyrenic and apyrenic “pitangueira” accessions

2021 ◽  
pp. 89-92
Author(s):  
Kelli Pirola ◽  
Marcelo Dotto ◽  
Américo Wagner Júnior ◽  
Ana Maria Castillo ◽  
Maria Herrero
Keyword(s):  
Fluorescence Intensity ◽  
Ploidy Level ◽  
Chromosomal Abnormalities ◽  
Diploid Species ◽  
Peak Height ◽  
Relative Fluorescence Intensity ◽  
Meiotic Behavior ◽  
Uv Laser ◽  
Multiple Parameters ◽  
Mature Leaves

Pitangueira (E. uniflora) is considered a diploid species with n = 11 and 2n = 22 chromosomes, in genotypes with the presence of seeds in the fruits. With the pitangueira production of apyrenic fruits existence, such behavior may be related to the ploidy level. The objective of this study was to determine the ploidy level of the pitangueira accessions producing fruit with and without seed, as well as to observe the meiotic behavior and possible chromosomal abnormalities. To check the ploidy level of the pitangueira, freshly expanded mature leaves were collected from the pyrenic pitangueira and from two pyrenic accessions, with suspensions of intact nuclei being prepared. Samples were analyzed in a flow cytometer equipped with multiple parameters data acquisition and UV laser. All analyses were performed using peak-height detection (>6000 fluorescent events, for example, nuclei, were analyzed per sample) and logarithmic amplification. The data were presented as histograms of the number of nuclei along the y-axis and the relative fluorescence intensity on the x-axis. The pyrenean pitangueira and other two pyrenic accessions were characterized as diploid.

scholarly journals The Sentinel Margin: Intraoperative Ex Vivo Specimen Mapping Using Relative Fluorescence Intensity

2019 ◽  
Vol 25 (15) ◽  
pp. 4656-4662 ◽  
Author(s):  
Stan van Keulen ◽  
Naoki Nishio ◽  
Andrew Birkeland ◽  
Shayan Fakurnejad ◽  
Brock Martin ◽  
...  
Keyword(s):  
Fluorescence Intensity ◽  
Ex Vivo ◽  
Relative Fluorescence Intensity

Two closely related Tulipa species with different ploidy levels show distinct germination rates

2020 ◽  
Vol 30 (1) ◽  
pp. 45-48
Author(s):  
Giovanni Astuti ◽  
Sandro Pratesi ◽  
Lorenzo Peruzzi ◽  
Angelino Carta
Keyword(s):  
Ploidy Level ◽  
Comparative Studies ◽  
Diploid Species ◽  
Tetraploid Species ◽  
Natural Conditions ◽  
Ploidy Levels ◽  
Final Length ◽  
Diploid Ancestor

AbstractIn the tetraploid Tulipa sylvestris we found larger and heavier seeds that germinated around 28 days earlier than those of the diploid ancestor Tulipa pumila. The faster germination of the tetraploid species is linked to the faster growth of embryos, which reached their final length much earlier than the embryos of the diploid species. In conclusion, we argue a cautionary approach when dealing with comparative studies on ploidy level and germination to avoid misinterpretation of results when set against the natural conditions.

Preliminary Evaluation of the Effects of Quenching and Inner-Filter on the Ratio Deconvolution of Fluorescence Data

1982 ◽  
Vol 36 (4) ◽  
pp. 460-466 ◽  
Author(s):  
Michael P. Fogarty ◽  
Isiah M. Warner
Keyword(s):  
Sample Preparation ◽  
Fluorescence Intensity ◽  
Preliminary Evaluation ◽  
Relative Fluorescence Intensity ◽  
Excitation Beam ◽  
Inner Filter Effects ◽  
Filter Mechanism ◽  
Filter Effects

The theory for the use of quenching as an aid in the ratio deconvolution of multicomponent fluorescence data is discussed. The advantage of quenching is that apparent changes in the relative fluorescence intensity of the components can be accomplished without extensive sample preparation. The disadvantage is that most quenchers exhibit significant absorption in the range of excitation wavelengths used for studying many fluorescent analytes. This absorption produces an attenuation of the excitation beam through an “inner-filter” mechanism. The problems associated with inner-filter effects are discussed.

Determination of Thiabendazole Residues in Pears by Solid-Phase Spectrofluorimetry

1994 ◽  
Vol 77 (6) ◽  
pp. 1651-1653 ◽  
Author(s):  
Luis Fermin Cápitan-Vallvey ◽  
Ramiro Avidad ◽  
Jose Luis Vilchez
Keyword(s):  
Acetic Acid ◽  
Detection Limit ◽  
Fluorescence Intensity ◽  
Analytical Procedure ◽  
Solid Phase ◽  
Solid Support ◽  
Relative Fluorescence Intensity ◽  
Buffer Solution ◽  
Silica Cell

Abstract An analytical procedure for determination of thiabendazole (TBZ) residues in pears is described. The method involves extracting the chemical from the chopped fruit with buffer solution (acetic acid–acetate, pH 4.6), use of Sephadex G-15 dextrantype gel as a solid support, and determination of TBZ by solid-phase spectrof luorimetry (SPF). The relative fluorescence intensity of the Sephadex G- 15 gel–TBZ system, packed in a 1 mm-thickness silica cell, was measured directly at λex = 303 nm and λem = 350 nm with a solid-phase attachment. The applicable concentration range was 5.0–20.0 ppb with a detection limit of 0.5 ppb. Recoveries were from 98.7 to 102.0% when 15.0 ppb of TBZ was added to the fruit.

HYBRIDIZATION BETWEEN ANNUAL AND PERENNIAL DIPLOID SPECIES OF HELIANTHUS

1978 ◽  
Vol 20 (4) ◽  
pp. 523-530 ◽  
Author(s):  
Ernest D. P. Whelan
Keyword(s):  
Interspecific Hybrids ◽  
Diploid Species ◽  
Paracentric Inversion ◽  
Sterile Hybrid ◽  
Meiotic Behavior ◽  
Perennial Species ◽  
Annual Species ◽  
Normal Pollen

Hybrid characteristics, fertility and meiosis are described for the first interspecific hybrids to be obtained from crosses of the two perennial species (2n = 34) Helianthus giganteus and H. maximiliani with the annual species H. annuus (2n = 34). A single, highly pollen-sterile hybrid, H. giganteus × H. annuus cv. Krasnodarets, had abnormal meiosis with meiocytes containing univalents and multivalents. It was completely female sterile. The three H. giganteus × wild H. annus and four H. maximiliani × wild H. annuus hybrids had less than 5% normal pollen, and meiotic behavior suggested the presence of at least three interchanges and a paracentric inversion. Small quantities of seed were obtained by backcrossing with H. annuus cv. Saturn.

scholarly journals Determining Ploidy Level and Nuclear DNA Content in Rubus by Flow Cytometry

2002 ◽  
Vol 127 (5) ◽  
pp. 767-775 ◽  
Author(s):  
Rengong Meng ◽  
Chad Finn
Keyword(s):  
Flow Cytometry ◽  
Pacific Northwest ◽  
Ploidy Level ◽  
Dna Content ◽  
Chromosome Numbers ◽  
Nuclear Dna ◽  
Diploid Species ◽  
Nuclear Dna Content ◽  
Dna Flow Cytometry ◽  
Ploidy Levels

Nuclear DNA flow cytometry was used to differentiate ploidy level and determine nuclear DNA content in Rubus. Nuclei suspensions were prepared from leaf discs of young leaves following published protocols with modifications. DNA was stained with propidium iodide. Measurement of fluorescence of 40 genotypes, whose published ploidy ranged from diploid to dodecaploid, indicated that fluorescence increased with an increase in chromosome number. Ploidy level accounted for 99% of the variation in fluorescence intensity (r2 = 0.99) and variation among ploidy levels was much higher than within ploidy levels. This protocol was used successfully for genotypes representing eight different Rubus subgenera. Rubus ursinus Cham. and Schldl., a native blackberry species in the Pacific Northwest, which has been reported to have 6x, 8x, 9x, 10x, 11x, and 12x forms, was extensively tested. Genotypes of R. ursinus were predominantly 12x, but 6x, 7x, 8x, 9x, 11x, and 13x forms were found as well. Attempts to confirm the 13x estimates with manual counts were unsuccessful. Ploidy level of 103 genotypes in the USDA-ARS breeding program was determined by flow cytometry. Flow cytometry confirmed that genotypes from crosses among 7x and 4x parents had chromosome numbers that must be the result of nonreduced gametes. This technique was effective in differentiating chromosome numbers differing by 1x, but was not able to differentiate aneuploids. Nuclear DNA contents of 21 diploid Rubus species from five subgenera were determined by flow cytometry. Idaeobatus, Chamaebatus, and Anaplobatus were significantly lower in DNA content than those of Rubus and Cylactis. In the Rubus subgenus, R. hispidus and R. canadensis had the lowest DNA content and R. sanctus had the highest DNA content, 0.59 and 0.75 pg, respectively. Idaeobatus had greater variation in DNA content among diploid species than the Rubus subgenus, with the highest being from R. ellipticus (0.69 pg) and lowest from R. illecebrosus (0.47 pg).

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