scholarly journals Silent point mutation in DsRed resulting in enhanced relative fluorescence intensity

BioTechniques ◽  
2004 ◽  
Vol 36 (2) ◽  
pp. 236-238 ◽  
Author(s):  
Maik Klasen ◽  
Matthias Wabl
Keyword(s):  
Point Mutation ◽  
Fluorescence Intensity ◽  
Relative Fluorescence Intensity

scholarly journals The Sentinel Margin: Intraoperative Ex Vivo Specimen Mapping Using Relative Fluorescence Intensity

2019 ◽  
Vol 25 (15) ◽  
pp. 4656-4662 ◽  
Author(s):  
Stan van Keulen ◽  
Naoki Nishio ◽  
Andrew Birkeland ◽  
Shayan Fakurnejad ◽  
Brock Martin ◽  
...  
Keyword(s):  
Fluorescence Intensity ◽  
Ex Vivo ◽  
Relative Fluorescence Intensity

Preliminary Evaluation of the Effects of Quenching and Inner-Filter on the Ratio Deconvolution of Fluorescence Data

1982 ◽  
Vol 36 (4) ◽  
pp. 460-466 ◽  
Author(s):  
Michael P. Fogarty ◽  
Isiah M. Warner
Keyword(s):  
Sample Preparation ◽  
Fluorescence Intensity ◽  
Preliminary Evaluation ◽  
Relative Fluorescence Intensity ◽  
Excitation Beam ◽  
Inner Filter Effects ◽  
Filter Mechanism ◽  
Filter Effects

The theory for the use of quenching as an aid in the ratio deconvolution of multicomponent fluorescence data is discussed. The advantage of quenching is that apparent changes in the relative fluorescence intensity of the components can be accomplished without extensive sample preparation. The disadvantage is that most quenchers exhibit significant absorption in the range of excitation wavelengths used for studying many fluorescent analytes. This absorption produces an attenuation of the excitation beam through an “inner-filter” mechanism. The problems associated with inner-filter effects are discussed.

Determination of Thiabendazole Residues in Pears by Solid-Phase Spectrofluorimetry

1994 ◽  
Vol 77 (6) ◽  
pp. 1651-1653 ◽  
Author(s):  
Luis Fermin Cápitan-Vallvey ◽  
Ramiro Avidad ◽  
Jose Luis Vilchez
Keyword(s):  
Acetic Acid ◽  
Detection Limit ◽  
Fluorescence Intensity ◽  
Analytical Procedure ◽  
Solid Phase ◽  
Solid Support ◽  
Relative Fluorescence Intensity ◽  
Buffer Solution ◽  
Silica Cell

Abstract An analytical procedure for determination of thiabendazole (TBZ) residues in pears is described. The method involves extracting the chemical from the chopped fruit with buffer solution (acetic acid–acetate, pH 4.6), use of Sephadex G-15 dextrantype gel as a solid support, and determination of TBZ by solid-phase spectrof luorimetry (SPF). The relative fluorescence intensity of the Sephadex G- 15 gel–TBZ system, packed in a 1 mm-thickness silica cell, was measured directly at λex = 303 nm and λem = 350 nm with a solid-phase attachment. The applicable concentration range was 5.0–20.0 ppb with a detection limit of 0.5 ppb. Recoveries were from 98.7 to 102.0% when 15.0 ppb of TBZ was added to the fruit.

scholarly journals Ploidia levels in pyrenic and apyrenic “pitangueira” accessions

2021 ◽  
pp. 89-92
Author(s):  
Kelli Pirola ◽  
Marcelo Dotto ◽  
Américo Wagner Júnior ◽  
Ana Maria Castillo ◽  
Maria Herrero
Keyword(s):  
Fluorescence Intensity ◽  
Ploidy Level ◽  
Chromosomal Abnormalities ◽  
Diploid Species ◽  
Peak Height ◽  
Relative Fluorescence Intensity ◽  
Meiotic Behavior ◽  
Uv Laser ◽  
Multiple Parameters ◽  
Mature Leaves

Pitangueira (E. uniflora) is considered a diploid species with n = 11 and 2n = 22 chromosomes, in genotypes with the presence of seeds in the fruits. With the pitangueira production of apyrenic fruits existence, such behavior may be related to the ploidy level. The objective of this study was to determine the ploidy level of the pitangueira accessions producing fruit with and without seed, as well as to observe the meiotic behavior and possible chromosomal abnormalities. To check the ploidy level of the pitangueira, freshly expanded mature leaves were collected from the pyrenic pitangueira and from two pyrenic accessions, with suspensions of intact nuclei being prepared. Samples were analyzed in a flow cytometer equipped with multiple parameters data acquisition and UV laser. All analyses were performed using peak-height detection (>6000 fluorescent events, for example, nuclei, were analyzed per sample) and logarithmic amplification. The data were presented as histograms of the number of nuclei along the y-axis and the relative fluorescence intensity on the x-axis. The pyrenean pitangueira and other two pyrenic accessions were characterized as diploid.

scholarly journals Flow Cytometry and Sperm Sexing in Animals

2018 ◽  
Vol 3 (3) ◽  
pp. 893-902
Author(s):  
Paulo Salinas
Keyword(s):  
Flow Cytometry ◽  
Light Beam ◽  
Fluorescence Intensity ◽  
Dna Content ◽  
Artificial Insemination ◽  
Stream Flow ◽  
Relative Fluorescence Intensity ◽  
Flow Through ◽  
Accuracy Speed ◽  
Current Procedure

Flow cytometry is a useful technology in the sexed sperm, which measures and analyzes simultaneously, multiple physical characteristics of the cell, as they flow in a stream flow, through a light beam. The measured properties are the size of a particle, relative internal granularity, relative complexity and relative fluorescence intensity. Currently, hundreds of calves have been gestated through artificial insemination with sexed sperm in animal production. Since 1992, flow cytometry has been used, a technique that allows spermatozoa X and Y differentiation by DNA content. There is no other practical technique for sperm sexing to keep sperm functionality. The objectives of this review are to explain: (1) why the sperm containing the X or Y chromosome are phenotypically similar, but differ among themselves, (2) the principles and procedures used for sexing sperm by flow cytometry and sorting ( 3) accuracy, speed and efficiency of current procedure sperm sexing, (4) sperm damage occurred during sperm sexing and consequently the effects on fertility.

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