scholarly journals Lymphatic filariasis: the importance of screening all peripheral blood smears in low power for detection of asymptomatic cases

2016 ◽  
Vol 5 (1) ◽  
pp. 350
Author(s):  
Sneha P. Chavarkar
Keyword(s):  
Low Power ◽  
Lymphatic Filariasis ◽  
Peripheral Blood ◽  
Blood Smear ◽  
Gradient Centrifugation ◽  
Case Series ◽  
Peripheral Blood Smear ◽  
High Morbidity ◽  
Polycarbonate Membrane ◽  
Asymptomatic Patients

Lymphatic filariasis caused by the mosquitoborne, lymphatic-dwelling nematodes Wuchereria bancrofti and Brugia malayi is still a common tropical parasitic disease and 120 million people are affected in the world, of which two-third are in Asia. They cause high morbidity and mortality among humans. Irreversible “elephantiasis” is the major clinical manifestation for LF. Detection of microfilaria in peripheral blood is important. In addition to simple thick and thin blood smear tests, concentration techniques are used: mainly density gradient centrifugation, haemolysis and filtration of the blood through a polycarbonate membrane, which retains the parasite. Diagnosis has been revolutionized with the availability of circulating filarial antigen (CFA) tests which are easy to perform but are costly. Diethylcarbamazine (DEC) is the drug of choice for treating lymphatic filariasis. In the light of this information, hereby presenting a case series of 4 asymptomatic patients who were diagnosed with filariasis on peripheral blood smear examination. The article emphasizes the importance of low power scanning of every peripheral blood smear, especially when the laboratory is not facilitated with costly methods to detect microfilaria.

Screening peripheral blood smear: A tale of the “TAIL”

2021 ◽  
pp. 004947552098638
Author(s):  
Neha Garg ◽  
Shilpi More ◽  
Parul Chopra ◽  
Richa Gupta ◽  
Mrinalini Kotru
Keyword(s):  
Early Diagnosis ◽  
Low Power ◽  
High Power ◽  
Peripheral Blood ◽  
Blood Smear ◽  
Peripheral Blood Smear ◽  
The Body

Examining a well-stained slide in a systematic manner is the key to being a good pathologist. For a blood smear, it involves examining first at low power (40× or 100×) for broader details and then going on to high power (400×) for finer details, from the tail end to the body of the slide. The ‘tail end’ is the key to early diagnosis.

scholarly journals ASSOCIATION BETWEEN ATYPICAL DEPOLARIZATION IN CELLDYN 00 AND THE PRESENCE OF PLASMODIUM SPP IN BLOOD IN Dr. SOETOMO HOSPITAL SURABAYA

2015 ◽  
Vol 2 (1) ◽  
pp. 15
Author(s):  
Jusak Nugraha
Keyword(s):  
Peripheral Blood ◽  
Malaria Infection ◽  
Blood Smear ◽  
Screening Method ◽  
Peripheral Blood Smear ◽  
Accurate Method ◽  
High Morbidity ◽  
Parasitic Disease ◽  
Malaria Parasites ◽  
Cross Sectional

Background: Malaria is a parasitic disease worldwide with a high morbidity and mortality. A rapid and accurate method is needed to detect the presence of malaria parasites in blood. A flagging system atypical depolarization (atypdep) in CBC results from Cell-Dyn 3200 has been related with malaria infection. Materials and Methods: An observational cross sectional approach with 48 samples obtained from inpatients of the Dr.Soetomo Hospital, Surabaya. Samples were screened by Cell-Dyn 3200 analyzer for atypdep flagging in CBC. Positive samples were later confirmed by microscope to detect malaria parasites. results: From 48 samples with atypdep flagging, 7 samples were malaria positive on peripheral blood smear (13.1%). Most frequent atypdep flagging was seen in malignancy (18.7%), and approximately 54.6% of the samples were not accompanied by fever symptoms. Lekositosis and anemia each were found in 20 samples (41.6%) and thrombocytopenia in 33.3%. conclusion: The presence of atypdep flagging in Cell-Dyn 3200 does not necessarily indicate the existence of malaria or it could be said that atypdep flagging is not always associated with presence of malaria infection. The usage of an atypdep flagging in non-endemic areas such as Surabaya is just an alert sign to evaluate malaria infection rather than a screening method to detect malaria.

Microfilaria: not always associated with eosinophilia

2019 ◽  
Vol 49 (3) ◽  
pp. 210-212
Author(s):  
Neha Garg ◽  
Shilpi More ◽  
Monika Sharma ◽  
Mrinalini Kotru
Keyword(s):  
Low Power ◽  
Peripheral Blood ◽  
Eosinophil Count ◽  
Blood Smear ◽  
Parasitic Infection ◽  
Peripheral Blood Smear ◽  
Buffy Coat ◽  
Blood Eosinophilia ◽  
Wet Mount ◽  
Peripheral Blood Eosinophilia

Filariasis is a parasitic infection seen predominantly in tropical and subtropical countries including India. In clinically suspected cases, examining a thick wet mount smear or a buffy coat film is most informative. In unsuspected cases, however, eosinophilia in a peripheral blood smear (PBS) may be the sole indicator of parasitaemia. A few cases of tissue microfilaria with the absence of peripheral blood eosinophilia (PBE) have been reported. Here, we report two cases of microfilaria in PBS in the absence of PBE. A routine screening of the tail end of all PBS at low power magnification is also advised as it may facilitate the detection of asymptomatic cases when there is a normal eosinophil count.

scholarly journals HUBUNGAN ANTARA FLAGGING ATYPDEP DI ALAT CELL-DYN 3200 DAN KEBERADAAN PLASMODIUM Spp DI DALAM DARAH PENDERITA DI RSUD DR.SOETOMO SURABAYA

2018 ◽  
Vol 17 (2) ◽  
pp. 97
Author(s):  
Esti Rohani ◽  
J Nugraha
Keyword(s):  
Peripheral Blood ◽  
Malaria Infection ◽  
Blood Smear ◽  
Malignant Disease ◽  
Screening Method ◽  
Peripheral Blood Smear ◽  
High Morbidity ◽  
Parasitic Disease ◽  
Malaria Parasites ◽  
Cross Sectional

Malaria is a parasitic disease worldwide with a high morbidity and mortality. A rapid and accurate methods is needed to detectthe presence of malaria parasites in blood. A flagging system atypical depolarization (atypdep) on CBC result from Cell-Dyn 3200instrument has been related with malaria infection. An observational cross sectional approach with a total of 48 samples were obtainedfrom inpatients in the Dr. Soetomo Hospital Surabaya. Samples were screened with Cell-Dyn 3200 analyzer for CBC found atypdepflagging. The positive samples were later confirmed by microscopic to detect malaria parasites. From 48 samples with atypdep flagging,seven samples were positive of malaria in peripheral blood smear (13.1%). Most frequent atypdep flagging was seen in malignant disease(18.7), an approximately 54.6% of the sample is not accompanied by symptoms of fever. Lekositosis and anemia were found in each of20 samples (41.6%) and thrombocytopenia in 33.3% of the samples. The presence of atypdep flagging does not necessarily indicate theexistence of malaria infection or it could be said that atypdep flagging is not always associated with the presence of malaria infection.The usage of an atypdep flagging on Cell-Dyn instrument in non-endemic areas such as Surabaya is just an alert sign to evaluate themalaria infection rather than a screening method to detect malaria.

scholarly journals Detection of cryptococcosis in peripheral blood smear:

2011 ◽  
Vol 1 (2) ◽  
pp. 116 ◽  
Author(s):  
Bhavna Nayal ◽  
S Niveditha ◽  
Veena ◽  
M Chethan
Keyword(s):  
Peripheral Blood ◽  
Blood Smear ◽  
Peripheral Blood Smear
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