scholarly journals Biofilm: An emergent form of bacterial life-a review

2021 ◽  
Vol 6 (2) ◽  
pp. 92-96
Author(s):  
Deepti Ancy Chacko ◽  
Neha Dhaded
Keyword(s):  
Root Canal ◽  
Biofilm Formation ◽  
Bacterial Eradication ◽  
Microbial Biofilms ◽  
Root Canal Irrigants ◽  
Adaptation Mechanisms

Biofilm formation is a method for bacteria to adapt for its survival, to put it another way, it act as a shield and prevents bacterial eradication. Microbial biofilms are one of the major reasons for progession of periradicular pathology. The article aims to concise and stratify the literature about, various factors that leads to biofilm formation their adaptation mechanisms, biofilms role in progression of peri-radicular infections, models developed to create biofilms, observation techniques of endodontic biofilms, and the effects of root canal irrigants and medicaments as well as lasers on endodontic biofilms.

Effect of root canal irrigants on push-out bond strength of endodontic sealers: a systematic review

2020 ◽  
pp. 1-21
Author(s):  
Lara Dotto ◽  
Gabriel Kalil Rocha Pereira ◽  
Alvin Tomm ◽  
Ataís Bacchi ◽  
Rafael Sarkis-Onofre
Keyword(s):  
Systematic Review ◽  
Bond Strength ◽  
Root Canal ◽  
Root Canal Irrigants ◽  
Endodontic Sealers ◽  
Push Out

scholarly journals Silver-Derived Antimicrobial Coatings for the Prevention of Microbial Biofilms in Metal Pipes

2021 ◽  
Vol 232 (8) ◽  
Author(s):  
M. Vela-Cano ◽  
C. Garcia-Fontana ◽  
F. Osorio ◽  
A. González-Martinez ◽  
J. González-López
Keyword(s):  
Biofilm Formation ◽  
Antimicrobial Properties ◽  
Pilot Scale ◽  
Biological Factors ◽  
Antimicrobial Coatings ◽  
Microbial Biofilms ◽  
Physical Chemical ◽  
Silver Zeolite ◽  
Metal Pipes ◽  
Pipeline Corrosion

AbstractBiodeterioration is one of the most important processes in metal pipeline corrosion, and it can be due to physical, chemical, and biological factors. Coatings rich in silver have been used to inhibit this undesirable phenomenon. In this study, the antimicrobial properties of several silver-containing products used as a coating in pipelines were determined on a pilot scale in order to evaluate the ability of silver to inhibit biofilm formation. The results showed that the coating with silver zeolite at a concentration of 2000 mg L–1 inhibited the formation of a microbial biofilm and prevented the biodeterioration process. Therefore, from our study, it can be concluded that silver zeolite shows greater protection capacity than other silver preparations and presents advantages in relation to other silver coatings that are currently available

Cytotoxic effects of different concentrations of neutral and alkaline EDTA solutions used as root canal irrigants

1999 ◽  
Vol 25 (1) ◽  
pp. 21-23 ◽  
Author(s):  
Elisabeth A. Koulaouzidou ◽  
John Margelos ◽  
Panagiotis Beltes ◽  
Alexander H. Kortsaris
Keyword(s):  
Root Canal ◽  
Cytotoxic Effects ◽  
Root Canal Irrigants

Optical properties of root canal irrigants in the 300–3,000-nm wavelength region

2013 ◽  
Vol 29 (5) ◽  
pp. 1557-1562 ◽  
Author(s):  
Maarten A. Meire ◽  
Dirk Poelman ◽  
Roeland J. De Moor
Keyword(s):  
Optical Properties ◽  
Root Canal ◽  
Wavelength Region ◽  
Root Canal Irrigants

Expression of Candidate Cold Stress and Metabolic Related Genes in Acidithiobacillus ferrivorans PQ33 Strain Using Ferrous Iron as Electron Donor

2017 ◽  
Vol 262 ◽  
pp. 368-371
Author(s):  
Gregory Guerra-Bieberach ◽  
Robert Ccorahua-Santo ◽  
Anika Eca ◽  
Jordan Bernaldo ◽  
Tito Sánchez ◽  
...  
Keyword(s):  
Candidate Genes ◽  
Low Temperatures ◽  
Biofilm Formation ◽  
Iron Oxidation ◽  
Trehalose Synthase ◽  
Adaptation Mechanisms ◽  
Diguanylate Cyclases ◽  
Cold Adaptations ◽  
Trehalose Synthesis ◽  
Andean Mining

The identification of genes involved in cold adaptations of psychrotolerant bacteria Acidithiobacillus ferrivorans is important for biomining processes that take place at low temperatures like Andean mining installations in Peru. We have performed relative quantification RT-qPCR on candidate genes to have a role in adaptations at low temperature (5°C). The candidate genes analyzed were six: Two trehalose synthesis pathway genes, trehalose synthase (treS) and malto-oligosiltrehalose trehalohydrolase (treZ) showing no overexpression at 5°C. Two diguanylate cyclases genes related to exopolymer synthesis and biofilm formation (designated as dgc-I and dgc-II in this paper) were overexpressed at 21°C. The rusA and rusB genes involved in iron oxidation showed no significant change for rusA and no expression for rusB gene in any of both conditions. Genes rpoC, gyrB and alaS were validated as reference genes. These results show congruency with trancriptomics studies about gene expression of A. ferrivorans. Furthermore, the trehalose synthesis genes show no overexpression at low temperatures suggesting that other cold adaptation mechanisms are involved.

scholarly journals UJI SITOTOKSISITAS EKSTRAK DAUN MIMBA (Azadirachta indica) TERHADAP SEL FIBROBLAS BHK 21

2017 ◽  
Vol 7 (1) ◽  
pp. 48
Author(s):  
Qurni Restiani ◽  
Mandojo Rukmo ◽  
Devi Eka Juniarti
Keyword(s):  
Antibacterial Activity ◽  
Optical Density ◽  
Azadirachta Indica ◽  
Root Canal ◽  
In Vitro Study ◽  
Cytotoxicity Test ◽  
Root Canal Irrigants ◽  
Neem Leaves ◽  
Alternative Material

Background: The leaves of neem (Azadirachta indicia) is one of herbal medicine that recommended as an alternative material of root canal irrigants. The active ingredients of neem leaves such as alkaloids, tannins, saponins and flavonoids has been proven to have antibacterial activity against E. faecalis. The ideal properties of an alternative material of root canal irrigants is not only have antibacterial activity but also is not toxic to the tissues, but the toxicity of neem leaves remains unclear until now. Objective: The aims of this study to determine the toxicity effect of neem leaves extract in specific concentration. Methods: This research was an experimental laboratory in vitro study of baby hamster kidney fibroblast (BHK-21). The neem leaves extract was made by maceration method using ethanol 96% and certain dilution performed to obtain various concentrations. Cytotoxicity test was conducted by MTT assay and the optical density was measured using ELISA reader at wavelength of 620 nm. Then, the optical density values were calculated using the formula for determining the number of survival fibroblasts after tested. Results: The percentage of survival fibroblast at concentration of 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95, and 100% in sequence were 71.3%, 64, 2%, 62%, 60.2%, 40.1%, 36.3%, 36.7%, 29%, 28.4%, 27.5%, and 25.6% . Conclusion: The extract of neem leaves (Azadirachta indica) has cytotoxic effects at concentration of 70% up to 100%.

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