scholarly journals Prevalence of keratinophilic fungi in the garden soil samples in Western India and study of its clinical impact

2021 ◽  
Vol 8 (1) ◽  
pp. 15-19
Author(s):  
Neha Agrawal ◽  
Madhu Yadav ◽  
Mukul Chaurasia ◽  
Vijaylatha Rastogi ◽  
Pushpanjali Verma ◽  
...  
2019 ◽  
Vol 18 (9) ◽  
pp. 1135-1154 ◽  
Author(s):  
Alejandra Giraldo ◽  
Margarita Hernández-Restrepo ◽  
Pedro W. Crous

Abstract During 2017, the Westerdijk Fungal Biodiversity Institute (WI) and the Utrecht University Museum launched a Citizen Science project. Dutch school children collected soil samples from gardens at different localities in the Netherlands, and submitted them to the WI where they were analysed in order to find new fungal species. Around 3000 fungal isolates, including filamentous fungi and yeasts, were cultured, preserved and submitted for DNA sequencing. Through analysis of the ITS and LSU sequences from the obtained isolates, several plectosphaerellaceous fungi were identified for further study. Based on morphological characters and the combined analysis of the ITS and TEF1-α sequences, some isolates were found to represent new species in the genera Phialoparvum, i.e. Ph. maaspleinense and Ph. rietveltiae, and Plectosphaerella, i.e. Pl. hanneae and Pl. verschoorii, which are described and illustrated here.


2012 ◽  
Vol 3 (1) ◽  
pp. 6 ◽  
Author(s):  
Sunil Kumar Deshmukh ◽  
Shilpa Amit Verekar

The parks of Mumbai are frequently visited by local residents every morning and evening. However, there are no reports on the occurrence of keratinophilic fungi in these areas. The purpose of this research was to study the occurrence of keratinophilic fungi in the public parks of Mumbai. One hundred soil samples were collected from five public parks: Kamla Nehru Park, Powai Garden, CD Deshmukh Garden, Five Gardens and Chota Kashmir. Keratinophilic fungi were isolated by the hair baiting technique using human hair as keratin bait. The cultures were identified using macroand micro-morphological features. Identification was also confirmed by the BLAST search of sequences of the ITS1-5.8S-ITS2 rDNA region against the NCBI/Genbank data and compared with deposited sequences. The ability of these fungi to use human hair was also evaluated by release of protein in liquid media. A total of 75 strains of keratinophilic fungi were recovered from 100 (75.0%) soil samples. The isolated fungi were composed of eleven species of eight genera: <em>Arthrographis kalrae</em>, A<em>uxarthron conjugatum</em>, <em>Chrysosporium indicum</em>, <em>C. queenslandicum</em>, <em>C. zonatum, Gymnascella dankaliensis</em>, <em>G. hyalinospora</em>,<em> Microsporum gypseum</em> (15.0%), <em>Myriodontium keratinophilum</em>, <em>Trichophyton mentagrophytes</em> and <em>Uncinocarpus reesii</em>. These fungi can release 148.8-307.6 μg/mL protein in liquid media when grown on human hair in shake flask culture and also decompose 16.2-38.6% of human hair after four weeks of incubation. Our study indicates that keratinophilic fungi are to be found in the soils of various public parks in Mumbai and that human hair can be a source of pathogenic fungi.


2020 ◽  
Vol 8 (3) ◽  
pp. 239-246
Author(s):  
Mainak Mukherjee ◽  
Sourav Chakraborty ◽  
Sahadeb Sarkar ◽  
Sumedha Saha ◽  
Soumya Majumder ◽  
...  

Tea is the most consumed beverages after water. In Terai and Dooars region of sub Himalayan West Bengal, India, tea plantations are around 150 years. Exploitation of tea garden soil over centuries has led to decline in soil fertility and crop productivity after all initiatives of external inputs. As nutrients play a crucial role in quality and quantity of manufactured tea, the present initiative has been designed to quantify the soil nutrient status of tea growing Terai and Dooars region. 79.62% (Terai) and 84.72% (Dooars) of the soil samples were found to have normal pH. 47.21% (Terai) and 49.3% (Dooars) of soil samples was found to have normal organic carbon. 12.03% and 20.82% of soil from Terai and Dooars showed low total nitrogen. Both the organic carbon and nitrogen in soil are positively correlated. Phosphorus (as P2O5) content of soil is extremely site and depth specific. Almost all samples of the study area showed high potassium (as K2O) content. Monoculture, change in rainfall pattern and inorganic additives have contributed enormous stress to soil but despite of all these odds the overall nutritional richness of these tea growing regions seems to be satisfactory.


Author(s):  
Jitendra Kumar ◽  
R. K. S. Kushwaha

Kanpur is a city which has huge number of leather product units and leather processing plants. These units are one of major contributors of keratinous waste and produces keratinous material as waste in the form of hairs, hides, dermis. During the present study 83 keratinophilic fungi were isolated from 40 soil samples of urban waste and cattle field habitat of various localities. From 20 samples of urban waste, 44 keratinophilic fungi were isolated, 39 fungi recorded from Cattle field. The frequency of genera Chrysosporium was recorded in urban waste (29.54%) and cattle field soil (20.51%). Maximum (13.83%) frequency was recorded in the case of Chrysosporium indicum in urban waste.


2014 ◽  
Vol 16 (1) ◽  
pp. 105-113
Author(s):  
Aleksandra Ihnatowicz

From the soil samples collected from beneath various banana plant, <i>Musa paradisiaca</i> L., 96 different species of soil fungi were isolated on medium: Ohio-Agar, Littmans-Agar, Martins Rose Bengal-Agar and identified. Four species of keratinophilic fungi were isolated by means of To-Ka-Va trap-hair method.


2020 ◽  
Vol 5 ◽  
pp. 1
Author(s):  
Mahulkar Ankita Vidyadhar Vaijayanti

Amphiphilic biosurfactants are surface-active biological molecules secreted by hydrocarbanoclastic microorganisms. Biosurfactants are eco-friendly, less toxic, biodegradable, and low-cost material, so it has more advantages over chemical surfactants. In this research, Pseudomonas spp., biosurfactant producing microorganisms isolated from different sources of soil samples. IS1, IS2, IS3, IS4 isolates obtained from Garden soil sample; Metal contaminated soil sample; Petroleum contaminated soil sample; Oil contaminated soil sample; respectively. Each isolates identified as Pseudomonas spp. Furthermore, screened for biosurfactant producers. Each isolate showed positive results for the hemolysis test, drop collapse test, oil displacement test, and emulsification test. All isolate incubated in mineral salt medium for biosurfactant production. Biosurfactant extracted from IS1, IS2, IS3, IS4 showed 35%, 65%, 20%, 52% emulsification index respectively. Antimicrobial activity of extracted biosurfactants against pathogenic microorganisms checked by agar cup method. IS2 isolate shows the highest antimicrobial activity among all. All isolate showed a higher zone of inhibition against gram-positive microorganisms than gram-negative microbes. The purpose of this study involves the assessment of the antimicrobial activity of biosurfactant producers from the soil environment.


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