Alcohol delamination of the corneal epithelium for recurrent corneal erosion syndrome

Purpose: to develop the optimal (safe and effective) method of diamond burr polishing of Bowman’s membrane (DBPBM) for the possible use it in the treatment of the recurrent corneal erosion syndrome (RCES) in the experiment.Material and methods. DBPBM in different modifications of the method and the mechanical deepithelization were performed in experiment on 19 human cadaver eyes. We use system Ophtho-Burr for DBPBM that includes a tip and ophthalmic burrs with diameter 1mm, 2.5 mm, 5 mm, varying the speed of rotation of burr. For 3 eyes — polishing with the application of burr with a diameter of 5 mm, the rotation frequency of 4000 rpm using 2 passes over the surface of the cornea. For 3 eyes — burr with a diameter of 2.5 mm, rotation frequency of 4000 rpm in 2 perpendicular passes. For 3 eyes — diameter burr 1 mm at speed of 8000 rpm in 2 perpendicular passes. For 3 eyes — diameter burr 1 mm at speed 4000 rpm with 4 passes along the surface of the cornea. For 4 eyes — diameter was 1 mm at speed of 4000 rpm in 2 perpendicular passes. For 3 the eyes was conducted mechanical deepithelization. Subsequent histopathological investigation of the cornea was performed by method of semi-thin section, with polychrome staining.Results. The obtained results of histological studies of DBPBM in different versions on cadaver cornea is allowed to choose the optimal variant of surgical intervention for use in clinical conditions. It consisted in a selection of burr with a diameter of 1 mm, the rotation frequency of the 4000 rpm and 2 perpendicular passes with a moderate compression of the cornea. The abovementioned technique of intervention allows removing completely the corneal epithelium with a basal membrane and save uniform in thickness, fully deepitelizationed Bowman’s membrane.Conclusion.Careful and accurate removal of dysplastic corneal epithelium in cases of the recurrent corneal erosion syndrome using proposed method in clinical conditions should contribute to the creation optimal environments for adequate reepithelization with steady epithelial-stromal adhesion. The remaining nearly intact Bowman’s membrane after procedure can prevent some postoperative complications such as haze and induced refractive disorders.

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Alcohol delamination of the corneal epithelium for recalcitrant recurrent corneal erosion syndrome: a prospective study of efficacy and safety

British Journal of Ophthalmology ◽

10.1136/bjo.2006.112912 ◽

2007 ◽

Vol 91 (7) ◽

pp. 908-911 ◽

Cited By ~ 27

Author(s):

R. P Singh ◽

D. Raj ◽

A. Pherwani ◽

R. Lagnado ◽

A. Abedin ◽

...

Keyword(s):

Prospective Study ◽

Corneal Epithelium ◽

Efficacy And Safety ◽

Corneal Erosion ◽

Recurrent Corneal Erosion ◽

A Prospective Study

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Galectin-3, IL-1A, IL-6, and EGF Levels in Corneal Epithelium of Patients With Recurrent Corneal Erosion Syndrome

Cornea ◽

10.1097/ico.0000000000002422 ◽

2020 ◽

Vol 39 (11) ◽

pp. 1354-1358

Author(s):

Tuba Candar ◽

Leyla Asena ◽

Husamadden Alkayid ◽

Dilek D. Altınörs

Keyword(s):

Corneal Epithelium ◽

Corneal Erosion ◽

Recurrent Corneal Erosion ◽

Galectin 3

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Faculty of 1000 evaluation for A randomised controlled trial of alcohol delamination and phototherapeutic keratectomy for the treatment of recurrent corneal erosion syndrome.

F1000 - Post-publication peer review of the biomedical literature ◽

10.3410/f.718026301.793482366 ◽

2013 ◽

Author(s):

Shahzad Mian ◽

Christopher Hood

Keyword(s):

Randomised Controlled Trial ◽

Controlled Trial ◽

Phototherapeutic Keratectomy ◽

Corneal Erosion ◽

Recurrent Corneal Erosion ◽

Randomised Controlled

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Treatment of recurrent corneal erosion syndrome using the combination of oral doxycycline and topical corticosteroid

Clinical and Experimental Ophthalmology ◽

10.1111/j.1442-9071.2007.01648.x ◽

2008 ◽

Vol 36 (1) ◽

pp. 8-12 ◽

Cited By ~ 27

Author(s):

Louis Wang ◽

Hughie Tsang ◽

Minas Coroneo

Keyword(s):

Topical Corticosteroid ◽

Oral Doxycycline ◽

Corneal Erosion ◽

Recurrent Corneal Erosion

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Kynurenic Acid Accelerates Healing of Corneal Epithelium In Vitro and In Vivo

Pharmaceuticals ◽

10.3390/ph14080753 ◽

2021 ◽

Vol 14 (8) ◽

pp. 753

Author(s):

Anna Matysik-Woźniak ◽

Waldemar A. Turski ◽

Monika Turska ◽

Roman Paduch ◽

Mirosław Łańcut ◽

...

Keyword(s):

Epithelial Cell ◽

Corneal Epithelium ◽

Kynurenic Acid ◽

Pharmaceutical Products ◽

Corneal Epithelial Cell ◽

Eye Drops ◽

Conjunctival Epithelium ◽

Corneal Erosion

Kynurenic acid (KYNA) is an endogenous compound with a multidirectional effect. It possesses antiapoptotic, anti-inflammatory, and antioxidative properties that may be beneficial in the treatment of corneal injuries. Moreover, KYNA has been used successfully to improve the healing outcome of skin wounds. The aim of the present study is to evaluate the effects of KYNA on corneal and conjunctival cells in vitro and the re-epithelization of corneal erosion in rabbits in vivo. Normal human corneal epithelial cell (10.014 pRSV-T) and conjunctival epithelial cell (HC0597) lines were used. Cellular metabolism, cell viability, transwell migration, and the secretion of IL-1β, IL-6, and IL-10 were determined. In rabbits, after corneal de-epithelization, eye drops containing 0.002% and 1% KYNA were applied five times a day until full recovery. KYNA decreased metabolism but did not affect the proliferation of the corneal epithelium. It decreased both the metabolism and proliferation of conjunctival epithelium. KYNA enhanced the migration of corneal but not conjunctival epithelial cells. KYNA reduced the secretion of IL-1β and IL-6 from the corneal epithelium, leaving IL-10 secretion unaffected. The release of all studied cytokines from the conjunctival epithelium exposed to KYNA was unchanged. KYNA at higher concentration accelerated the healing of the corneal epithelium. These favorable properties of KYNA suggest that KYNA containing topical pharmaceutical products can be used in the treatment of ocular surface diseases.

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