scholarly journals Phosphomannose isomerase gene for selection in lettuce (Lactuca sativa L.) transformation.

2010 ◽  
Vol 57 (1) ◽  
Author(s):  
Jindrich Bríza ◽  
Nina Růzicková ◽  
Hana Niedermeierová ◽  
Jana Dusbábková ◽  
Josef Vlasák
Keyword(s):  
Lactuca Sativa ◽  
Transcriptional Activity ◽  
Northern Analysis ◽  
Selection System ◽  
Phosphomannose Isomerase ◽  
Lactuca Sativa L ◽  
Clonal Origin ◽  
Selection Agent ◽  
Very High ◽  
Specific Mrna

A positive selection system using phosphomannose isomerase was employed for Agrobacterium tumefaciens mediated transformation of lettuce (Lactuca sativa L. var. 'Achát'). It was shown that the mannose-based selection system works very well with the lettuce genotype used, reaching up to 25% transformation efficiency on the medium with 20 g/L mannose and 20 g/L sucrose. The best transformation efficacy with the commonly-used kanamycin at 100 mg/L as a selection agent was 21%. Southern blot analyses of thirteen chosen mannose-resistant regenerants revealed that some of them have clonal origin, about one-half harbour a single T-DNA copy and one plant contains an incomplete T-DNA segment with only the left part of T-DNA with the pmi gene present in the genomic DNA. The following Northern analysis showed transcriptional activity of the introduced pmi gene in all plants analysed with very high differences in the level of pmi specific mRNA. The results demonstrate that both mannose and kanamycin provide comparable transformation efficiencies in our lettuce genotype. An alternative selection method with mannose as a selection agent is now available for lettuce transgenosis.

scholarly journals Effects of Ethylene Diamine Disuccinic Acid (EDDS) on lead (Pb2+) and cadmium (Cd2+) uptake by lettuce (Lactuca sativa L.) seedlings planted in hydroponic solutions

2020 ◽  
Vol 12 (1) ◽  
pp. 426-434
Author(s):  
M.S. Dagari ◽  
M.S. Musa ◽  
F.M. Maude
Keyword(s):  
Lactuca Sativa ◽  
Research Work ◽  
Ethylene Diamine ◽  
High Concentration ◽  
Constant Concentration ◽  
Lactuca Sativa L ◽  
Dry Ashing ◽  
Cadmium And Lead ◽  
Hydroponically Grown ◽  
Very High

This research work was carried out to investigate the effects of ethylene diamine disuccinate (EDDS) on the absorption of cadmium and lead by hydroponically grown lettuce (Lactuca sativa L.). Samples were prepared through digestion (dry ashing) method and the analytes quantitatively analyzed using Atomic Absorption Spectrophotometric (AAS) technique. The effects of EDDS on Pb2+ and Cd2+ uptake by lettuce (Lactuca sativa L.) seedlings replanted in hydroponic solutions for 72 hr in a greenhouse were investigated. Two months old seedlings were exposed to various doses of Pb2+ and Cd2+ at constant concentration of EDDS. For unchelated treatments, increase in concentrations of Pb2+ and Cd2+ in the solution increases the uptake by the plants relative to control.  Pb2+ contents in the roots showed more accumulation than in shoots (p<0.05) while Cd2+ content in the shoots was higher than in roots.  Addition of 0.0025M EDDS to the solutions of Pb2+ and Cd2+ inhibits the uptake of the metal ions at the following concentrations (0.0025, 0.005 and 0.0075 mg/L) when compared to unchelated treatments of same concentrations. At 0.025 mg/L of Pb2+ and Cd2+, chelation substantially enhances their uptakes in both shoot and root of the plant (p<0.05).  Compared to the unchelated treatments, chelation suppresses the translocation of Pb2+ and Cd2+ to the shoots of the plant (p<0.05), but there was a better translocation of Pb2+ (1.257) and Cd2+ (0.953) at 0.025 mg/L, thus indicating that, at very high concentration of the metals the plant was hyper-accumulator. For hydroponic solutions containing the combination of both metals; Pb2+ + Cd2+, Pb2+ uptake was more in the roots than in the shoots while Cd2+ content in the shoots showed more absorption than the roots for both chelated and unchelated treatments. Also, it was observed that chelation suppresses the uptake of Pb2+ and Cd2+ in the presence of one another by both root and shoot. Furthermore, increasing the concentrations of Pb2+ + Cd2+ in chelated treatments of hydroponic solutions results to a better translocation of Pb2+ while the translocation of Cd2+ to the shoot was enhanced. Generally, addition of EDDS to the hydroponic mixtures affected to a large extent the availability of Pb2+ and Cd2+ to the growing seedlings. Keywords: Ethylene diamine disuccinate, greenhouse, hydroponic solution, lettuce, metals.  

scholarly journals Evaluation of the Phosphomannose Isomerase-Based Selection System for Genetic Transformation of Sweetpotato

2015 ◽  
Vol 9 (3) ◽  
pp. 46-53 ◽  
Author(s):  
Wilton Mbinda ◽  
Sylvia Nawiri ◽  
Makenzi Nzaro ◽  
Benson Kinyagia ◽  
Allan Mgutu ◽  
...  
Keyword(s):  
Cell Growth ◽  
Genetic Transformation ◽  
Life Sciences ◽  
Stem Explants ◽  
Selection System ◽  
Phosphomannose Isomerase ◽  
Public Concern ◽  
Effective Selection ◽  
Minimal Concentration ◽  
Selection Agent

In response to increased public concern on antibiotic or herbicide resistance genes usage in genetically modified plants, mannose was evaluated as selectable agent for the genetic transformation of sweetpotato. Nontransformed sweetpotato stem explants of cv. KSP36 were cultured on media containing various combinations and concentrations sucrose and mannose ranging from 0 to 30 g/l. Likewise, efficacy of hygromycin and kanamycin as selection agents for transformation of sweetpotato was evaluated on media containing varying concentrations of antibiotics ranging 0 to 100 mg/l. Selection agent effectiveness was determined by detecting the minimal concentration of the selection agent required to fully inhibit sweetpotato calli growth. Hygromycin was the most effective selection agent as it inhibited cell growth at concentrations above 20 mg/l. Kanamycin was moderately effective as it inhibited cell growth at 60 mg/l. Sweetpotato calli were able to grow and even produce embryos even when mannose was the only source of carbohydrates.DOI: http://dx.doi.org/10.3126/ijls.v9i3.12466 International Journal of Life Sciences 9 (3): 2015; 46-53

Pengaruh Jenis dan Konsentrasi Pupuk Organik Cair terhadap Pertumbuhan dan Produksi Tanaman Selada (Lactuca Sativa l.) secara Hidroponik

2017 ◽  
Vol 2 (01) ◽  
pp. 115-127
Author(s):  
Siti Hilalliyah ◽  
Intan Sari ◽  
Zahlul Ikhsan
Keyword(s):  
Lactuca Sativa ◽  
Lactuca Sativa L

Sistem hidroponik memungkinkan sayuran ditanam di daerah yang kurang subur dan daerah sempit yang padat penduduknya. Alasan penerapan teknik hidroponik yang utama adalah karena terbatasnya lahan pertanian yang produktif untuk memenuhi kebutuhan manusia yang semakin banyak tiap tahunnya, sehingga dibutuhkan suatu terobosan baru untuk memecahkan masalah tersebut. Penelitian ini telah dilaksanakan pada bulan November 2015 sampai bulan Januari 2016 yang bertempat di kampus Fakultas Pertanian Jl. Propinsi Kecamatan Tembilahan Hulu Kabupaten Indragiri Hilir Propinsi Riau.Metode penelitian yang digunakan adalah Rancangan Acak Lengkap (RAL) pola faktorial yang terdiri dari 2 faktor. Faktor pertama adalah jenis POC (N) yang terdiri dari 3 taraf perlakuan yaitu POC Jus Bumi, POC Bonggol Pisang danPOC Limbah Sayuran. Faktor kedua adalah konsentrasi larutan (K) yang terdiri dari 3 taraf perlakuan yaitu 150 ppm, 200 ppm dan 250 ppm. Parameter pengamatan adalah Tinggi Tanaman , Jumlah Daun , Luas Daun , Diameter Batang, Panjang akar ,Volume Akar, Kadar Air, dan Bobot Hasil.Hasil pengamatan menunjukkan bahwa perlakuan jenis POC jus bumi dengan konsentrasi larutan 150 ppm memberikan pertumbuhan dan produksi terbaik untuk tanaman selada secara hidroponik sistem wick. Perlakuan jenis POC limbah sayuran dengan konsentrasi 250 ppm memberikan pertumbuhan dan produksi terbaik selain POC jus bumi sehingga dapat dijadikan sumber POC alternatif.

KHẢO SÁT DƯ LƯỢNG NITRAT VÀ KIM LOẠI NẶNG (Cu, Pb, Zn) TRONG CÂY RAU XÀ LÁCH (Lactuca sativa L.) TRỒNG Ở PHƯỜNG HƯƠNG LONG - THÀNH PHỐ HUẾ

2015 ◽  
Vol 100 (1) ◽  
Author(s):  
Nguyễn Minh Trí ◽  
Nguyễn Hạnh Trinh ◽  
Nguyễn Thị Hoàng Phương
Keyword(s):  
Lactuca Sativa ◽  
Viet Nam ◽  
Lactuca Sativa L ◽  
Tu 45

Xà lách (Lactuca sativa L.) là một loại rau ăn lá quan trọng có giá trị dinh dưỡng và kinh tế cao. Cây Xà lách có đặc điểm là loại rau ngắn ngày, có thời gian sinh trưởng ngắn khoảng từ 45 - 55 ngày, có thể phát triển tốt trên nhiều loại đất, là loại rau ăn sống được sử dụng rất phổ biến trong bữa ăn hàng ngày của người dân Việt Nam nên nó được trồng quanh năm, do vậy vấn đề về chất lượng lại càng phải được quan tâm nhiều hơn. Bài báo này giới thiệu kết quả phân tích về dư lượng nitrat và các kim loại nặng (Cu, Pb, Zn) trong rau Xà lách vụ Xuân - Hè 2012 - 2013 ở phường Hương Long - thành phố Huế. Kết quả nghiên cứu cho thấy: đất trồng rau Xà lách tại phường Hương Long – thành phố Huế đạt tiêu chuẩn về hàm lượng kim loại nặng (Pb, Zn) theo QCVN 03:2008/BTNMT, nhưng hàm lượng Cu và nitrat là khá cao. Rau Xà lách thành phẩm có dư lượng nitrat cao hơn 1,21% so với quy định và các kim loại nặng (Pb, Zn, Cu) tồn dư trong rau lại ở mức cao và vượt tiêu chuẩn cho phép nhiều lần sẽ ảnh hưởng đến sức khỏe của người sử dụng.

EFEITO DO LIXIVIADO DE VERMICOMPOSTO EM DIFERENTES DILUIÇÕES, COMO BIOFERTILIZANTE PARA A CULTURA DA ALFACE (LACTUCA SATIVA L.)

2018 ◽  
Author(s):  
Ferreira Gabriel Menezes ◽  
Souza Antonio Tassio de Oliveira ◽  
Souza Alisson Silva de ◽  
Gomes Igor Thiago dos Santos ◽  
Cunha Denise de Andrade
Keyword(s):  
Lactuca Sativa ◽  
Lactuca Sativa L

PROMOCIÓN DEL CRECIMIENTO DE PLANTAS DE LECHUGA (Lactuca sativa L.) CON DOSIS SUBLETALES DE OZONO APLICADAS AL MEDIO DE CULTIVO

2015 ◽  
Vol 38 (4) ◽  
pp. 405
Author(s):  
Jorge A. Vázquez-Ybarra ◽  
Cecilia B. Peña-Valdivia ◽  
Carlos Trejo ◽  
Albino Villegas-Bastida ◽  
Sergio Benedicto-Valdéz ◽  
...  
Keyword(s):  
Lactuca Sativa ◽  
Lactuca Sativa L

Las plantas han desarrollado un conjunto de mecanismos morfológicos, bioquímicos y fisiológicos de respuesta a los cambios ambientales de O3. El objetivo de este estudio fue evaluar el efecto de dosis subletales de O3, aplicadas al medio de cultivo, en el crecimiento de plantas de lechuga (Lactuca sativa L.). Con base en el fenómeno denominado hormesis, la hipótesis fue que existe una dosis umbral de O3 que modifica positivamente el metabolismo de las plantas e incrementan su crecimiento y productividad. En un sistema hidropónico de plantas en flotación se evaluaron nueve dosis entre 0.53 y 59.40 mg L-1, aplicadas semanalmente, y se compararon con un testigo sin O3. Las variables del crecimiento que se cuantificaron fueron: diámetro del tallo, altura del tallo, altura de hoja, número total de hojas, longitud de raíz, peso fresco de raíz, biomasa seca de raíz, peso fresco y biomasa del vástago. Las plantas completaron su ciclo de crecimiento sin algún daño en el crecimiento con concentraciones entre 0.53 y 5.94 mg de O3 L-1, pero 20, 40 y 60 mg L-1 fueron letales en plantas de cinco semanas de edad. Estas dosis causaron oscurecimiento y necrosis acelerados de los tejidos foliares y muerte, los cambios de color de las hojas fueron evidentes desde los 20 min posteriores a la única aplicación de O3. Además, las dosis de 2.66 y 3.96 mg L-1 incrementaron significativamente (P ≤ 0.05) el diámetro del tallo, la biomasa fresca en la raíz y el vástago y la biomasa seca en la raíz y el vástago, en promedio 16, 23, 15, 89 y 11 % con respecto al testigo, después de 10 semanas de crecimiento.

scholarly journals EC Sensitivity of Hydroponically-Grown Lettuce (Lactuca sativa L.) Types in Terms of Nitrate Accumulation

Agriculture ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 315
Author(s):  
Noémi Kappel ◽  
Ildikó Fruzsina Boros ◽  
Francia Seconde Ravelombola ◽  
László Sipos
Keyword(s):  
Lactuca Sativa ◽  
Nitrate Content ◽  
Saline Condition ◽  
Fresh Weight ◽  
Nitrate Level ◽  
Nitrate Accumulation ◽  
Strong Negative Correlation ◽  
Lactuca Sativa L ◽  
Hydroponically Grown ◽  
European Markets

The goal of this research was to investigate the effect of electrical conductivity (EC) levels of the nutrient solution on the fresh weight, chlorophyll, and nitrate content of hydroponic-system-grown lettuce. The selected cultivars are the most representative commercial varieties grown for European markets. Seven cultivars (‘Sintia,’ ‘Limeira,’ ‘Corentine,’ ‘Cencibel,’ ‘Kiber,’ ‘Attiraï,’ and ‘Rouxaï’) of three Lactuca sativa L. types’ (butterhead, loose leaf, and oak leaf) were grown in a phytotron in rockwool, meanwhile the EC level of the nutrient solutions were different: normal (<1.3 dS/m) and high (10 dS/m). The plants in the saline condition had a lower yield but elevated chlorophyll content and nitrate level, although the ‘Limeira’ and ‘Cencibel’ cultivars had reduced nitrate levels. The results and the special characteristic of the lollo-type cultivars showed that the nitrate level could be very different due to salinity (‘Limeira’ had the lowest (684 µg/g fresh weight (FW)) and ‘Cencibel’ had the highest (4396 µg/g FW)). There was a moderately strong negative correlation (−0.542) in the reverse ratio among the chlorophyll and nitrate contents in plants treated with a normal EC value, while this relationship was not shown in the saline condition. Under the saline condition, cultivars acted differently, and all examined cultivars stayed under the permitted total nitrate level (5000 µg/g FW).

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