scholarly journals Study Effect of Vitamin D on the Immunopathology Responses of the Bronchi in Murine Model of Asthma

2021 ◽  
Author(s):  
Linlin Feng ◽  
Tingting Meng ◽  
Yunyun Qi ◽  
Seyyed Shamsadin Athari ◽  
Xiaoyun Chen
Keyword(s):  
Vitamin D ◽  
Allergic Asthma ◽  
Goblet Cell ◽  
Murine Model ◽  
Bronchoalveolar Lavage Fluid ◽  
Lavage Fluid ◽  
Cell Hyperplasia ◽  
Goblet Cell Hyperplasia ◽  
Asthmatic Patients ◽  
Genetic And Environmental Factors

  Allergic asthma is a complicated respiratory problem characterized by airway inflammation, airway hyperresponsiveness (AHR), breathlessness, mucus hyper-secretion, and goblet cell hyperplasia. Asthma is controlled by genetic and environmental factors. Allergy is the main trigger of asthma and is mediated by Th2 cytokines along with IgE production. Vitamin D (Vit D) is the main supplementary factor for the immune system. In the present study, we investigated the effect of Vit D on the exacerbation of allergic asthma. A murine model of allergic asthma was induced by ovalbumin (OVA) in four of five groups of studied female BALB/c mice (each group, n=20). One group was considered as control. Of OVA-induced mice, two groups received Vit D via oral (10,000 IU/kg diet) or intranasal (inhalation) forms (30 min on days 25, 27, and 29), and the third group received budesonide. At least, AHR, the levels of IL-4, IL-5, IL-13, and INF-g in bronchoalveolar lavage fluid (BALF), serum IgE and histamine, IL-25 and IL-33 gene expression, as well as histopathology study of the lung were done. The Penh values, type2 Cytokines in BALF (in both protein and molecular levels), total IgE and histamine, perivascular and peribronchial inflammation, goblet cell hyperplasia, and mucus hypersecretion decreased significantly in both oral and intranasal Vit D-treated asthmatic mice groups, especially on day 38 of orally treated mice. Here, we found Vit D as a promising agent in control of allergic asthma with a remarkable ability to decrease the severity of inflammation. Therefore, Vit D sufficiency is highly recommended in asthmatic patients.

scholarly journals A Synthetic Curcuminoid Analogue, 2,6-Bis-4-(Hydroxyl-3-Methoxybenzylidine)-Cyclohexanone (BHMC) Ameliorates Acute Airway Inflammation of Allergic Asthma in Ovalbumin-Sensitized Mice

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Chau Ling Tham ◽  
Sin Yee Yeoh ◽  
Chun Hao Ong ◽  
Hanis Hazeera Harith ◽  
Daud Ahmad Israf
Keyword(s):  
Bronchoalveolar Lavage ◽  
Airway Inflammation ◽  
Allergic Asthma ◽  
Goblet Cell ◽  
Bronchoalveolar Lavage Fluid ◽  
Intraperitoneal Administration ◽  
Lavage Fluid ◽  
Cell Hyperplasia ◽  
Goblet Cell Hyperplasia ◽  
Cellular Models

2,6-Bis-(4-hydroxyl-3-methoxybenzylidine) cyclohexanone (BHMC), a synthetic curcuminoid analogue, has been shown to exhibit anti-inflammatory properties in cellular models of inflammation and improve the survival of mice from lethal sepsis. We further evaluated the therapeutic effect of BHMC on acute airway inflammation in a mouse model of allergic asthma. Mice were sensitized and challenged with ovalbumin (OVA), followed by intraperitoneal administration of 0.1, 1, and 10 mg/kg of BHMC. Bronchoalveolar lavage fluid, blood, and lung samples were collected, and the respiratory function was measured. OVA sensitization and challenge increased airway hyperresponsiveness (AHR) and pulmonary inflammation. All three doses of BHMC (0.1-10 mg/kg) significantly reduced the number of eosinophils, lymphocytes, macrophages, and neutrophils, as well as the levels of Th2 cytokines (IL-4, IL-5 and IL-13) in bronchoalveolar lavage fluid (BALF) as compared to OVA­challenged mice. However, serum level of IgE was not affected. All three doses of BHMC (0.1-10 mg/kg) were effective in suppressing the infiltration of inflammatory cells at the peribronchial and perivascular regions, with the greatest effect observed at 1 mg/kg which was comparable to dexamethasone. Goblet cell hyperplasia was inhibited by 1 and 10 mg/kg of BHMC, while the lowest dose (0.1 mg/kg) had no significant inhibitory effect. These findings demonstrate that BHMC, a synthetic nonsteroidal small molecule, ameliorates acute airway inflammation associated with allergic asthma, primarily by suppressing the release of inflammatory mediators and goblet cell hyperplasia to a lesser extent in acute airway inflammation of allergic asthma.

Triptolide suppresses airway goblet cell hyperplasia and Muc5ac expression via NF-κB in a murine model of asthma

2015 ◽  
Vol 64 (1) ◽  
pp. 99-105 ◽  
Author(s):  
Ming Chen ◽  
Zhiqiang Lv ◽  
Wei Zhang ◽  
Linjie Huang ◽  
Xiaoling Lin ◽  
...  
Keyword(s):  
Goblet Cell ◽  
Murine Model ◽  
Cell Hyperplasia ◽  
Goblet Cell Hyperplasia

The comparison of histopathological characteristics of polyps in asthmatic and nonasthmatic patients

2009 ◽  
Vol 140 (5) ◽  
pp. 748-751 ◽  
Author(s):  
Mojtaba Mohammadi Ardehali ◽  
Amin Amali ◽  
Mehdi Bakhshaee ◽  
Ziaodin Madani ◽  
Mandana Amiri
Keyword(s):  
Basement Membrane ◽  
Chronic Rhinosinusitis ◽  
Goblet Cell ◽  
Lymphocytic Infiltration ◽  
Clinical Aspects ◽  
Membrane Thickness ◽  
Cell Hyperplasia ◽  
Basement Membrane Thickness ◽  
Goblet Cell Hyperplasia ◽  
Asthmatic Patients

Introduction: Considering the different clinical aspects of polyps in asthmatic and nonasthmatic patients, we aimed to explore their histopathological characteristics. Material and Methods: Twenty-five asthmatic patients and 25 nonasthmatic patients with polypoid chronic rhinosinusitis (29 male, 21 female; mean age 41.3 ± 13.27; range 15-78 years) were enrolled in the study to be compared on the basis of histopathological characteristics. They were compared according to the following seven light microscopic findings: basement membrane thickness, goblet cell hyperplasia, subepithelial edema, submucous gland formation, eosinophilic infiltration, lymphocytic infiltration, and polymorphonuclear infiltration. Results: Basement membrane thickening, goblet cell hyperplasia, and eosinophilic and lymphocytic infiltration were more prominent in the asthmatic compared with the nonasthmatic group ( P < 0.05), whereas polymorphonuclear infiltration was more prominent in nonasthmatics ( P < 0.05). No statistically significant differences were found between the two groups with regard to submucosal gland hyperplasia or subepithelial edema. Conclusion: Asthmatic patients present histopathological characteristics of a marked chronic inflammatory reaction, which might explain the negative effect on chronic rhinosinusitis outcome and the severity of the disease in this group.

scholarly journals Recurrent Milk Aspiration Produces Changes in Airway Mechanics, Lung Eosinophilia, and Goblet Cell Hyperplasia in a Murine Model

2000 ◽  
Vol 48 (6) ◽  
pp. 776-781 ◽  
Author(s):  
Ibrahim A Janahi ◽  
Okan Elidemir ◽  
Felix R Shardonofsky ◽  
Mutasim N Abu-Hassan ◽  
Leland L Fan ◽  
...  
Keyword(s):  
Goblet Cell ◽  
Murine Model ◽  
Cell Hyperplasia ◽  
Goblet Cell Hyperplasia ◽  
Airway Mechanics ◽  
Lung Eosinophilia

scholarly journals Simvastatin inhibits goblet cell hyperplasia and lung arginase in a mouse model of allergic asthma: a novel treatment for airway remodeling?

2010 ◽  
Vol 156 (6) ◽  
pp. 335-349 ◽  
Author(s):  
Amir A. Zeki ◽  
Jennifer M. Bratt ◽  
Michelle Rabowsky ◽  
Jerold A. Last ◽  
Nicholas J. Kenyon
Keyword(s):  
Mouse Model ◽  
Allergic Asthma ◽  
Goblet Cell ◽  
Airway Remodeling ◽  
Cell Hyperplasia ◽  
Goblet Cell Hyperplasia ◽  
Novel Treatment

Antiasthmatic Effects of Gleditsia sinensis in an Ovalbumin-Induced Murine Model of Asthma

2011 ◽  
Vol 30 (5) ◽  
pp. 528-537 ◽  
Author(s):  
Mee-Young Lee ◽  
In-Sik Shin ◽  
Chang-Seob Seo ◽  
Heykyung Ha ◽  
Hyeun-Kyoo Shin
Keyword(s):  
Allergic Asthma ◽  
Murine Model ◽  
Immunoglobulin E ◽  
Ethanolic Extract ◽  
Lavage Fluid ◽  
Dependent Manner ◽  
Cell Hyperplasia ◽  
Inflammatory Infiltration ◽  
Gleditsia Sinensis

This study evaluated the antiasthmatic effects of Gleditsia sinensis ethanolic extract (GSEE) and its underlying mechanisms, using an in vivo murine model of asthma. Female BALB/c mice were sensitized, challenged with ovalbumin, and then examined for asthmatic reactions. The results showed that GSEE exerted profound inhibitory effects on the accumulation of eosinophils in the airways and reduced the levels of interleukin (IL)-4 and IL-5 in bronchoalveolar lavage fluid (BALF) and immunoglobulin E (IgE) in BALF and plasma. Gleditsia sinensis ethanolic extract also suppressed the production of reactive oxygen species in BALF and inflammatory infiltration, in a dose-dependent manner, and it inhibited goblet-cell hyperplasia in lung tissue. Thus, GSEE shows antiasthmatic effects in a murine model of allergic asthma, which appeared to be mediated partially by the reduction of oxidative stress and airway inflammation. These results indicate that GSEE could be an effective novel therapeutic agent for the treatment of allergic asthma.

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