scholarly journals Selective screening and characterization of plant growth promoting bacteria for growth enhancement of tomato, Lycopersicon esculentum

2021 ◽  
Author(s):  
Shiva Yavarian ◽  
Parvaneh Jafari ◽  
Neda Akbari ◽  
Mohammad Mehdi Feizabadi
Keyword(s):  
Lycopersicon Esculentum ◽  
Plant Growth ◽  
Tomato Plant ◽  
Phosphate Solubilization ◽  
Shoot Length ◽  
Plant Production ◽  
Plant Growth Promoting Bacteria ◽  
Dependent Manner ◽  
Plant Growth Promoting ◽  
Growth Promoting

Background and Objectives: Plant Growth-promoting Bacteria (PGPB) can replace the dangerous chemical fertilizers and pesticides. The aim of this study was to isolate the PGPBs for Lycopersicon esculentum plant and to determine the appropriate volume for inoculation. Materials and Methods: Plants samples were collected from tomato fields. Nitrogen fixing-PGPBs were isolated from rhizoplane and rhizosphere. Five isolates were screened based on their growth abilities and examined for PGPB traits including phosphate solubilization, and IAA, ammonia and HCN production. After high cell density cultivation, the cells were separated by centrifugation and freeze dried after resuspension in cryoprotectant. The powders were inoculated into sterile soil with a dose of 106 , 107 and 108 CFUs/g. Tomato (Lycopersicon esculentum) seeds were sown in soil and after 42 days the shoot length was measured. Results: Most of the potent PGPBs with high growth capacity were isolated from rhizoplane. Maximum phosphate solubilization was 289.7 µg/ml by NFB12 which isolated from rhizoplane. This strain produced the maximum level of IAA. NFB12 produced ammonia without the ability of production of HCN. This strain enhanced shoot length in dosed dependent manner. Surprisingly, inoculation of soil with 108 CFUs/g dramatically decreased the shoot length by 21%. Based on molecular approach NFB12 was identified as Bacillus megaterium. Conclusion: Isolation of specific PGPBS is recommended for sustainable plant production. Our results showed that NBF12 improves tomato plant growth and its effect on tomato plant growth is does dependent. Maximum growth rate of tomato was observed with 107 CFUs/g soil inoculation of NFB12 while higher inoculation showed negative effect.

scholarly journals Impact of Lysinibacillus macroides, a potential plant growth promoting rhizobacteria on growth, yield and nutritional value of tomato Plant (Solanum lycopersicum L. f1 hybrid Sachriya)

2021 ◽  
Vol 8 (2) ◽  
Author(s):  
K S Jyolsna ◽  
N Bharathi ◽  
L Riyaz Ali ◽  
K A Paari
Keyword(s):  
Plant Growth ◽  
Solanum Lycopersicum ◽  
Tomato Plant ◽  
Shoot Length ◽  
Plant Growth Promoting Bacteria ◽  
F1 Hybrid ◽  
Solanum Lycopersicum L ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Organic Compost

Plant growth promoting bacteria enhance the growth in plants by solubilizing insoluble minerals, producing phytohormones and by secreting enzymes that resist pathogen attack. The present study was aimed at identifying the potential of Lysinibacillus macroides isolated from pea plant possessing rich microbial rhizobiome diversity in promoting the growth of tomato plant (Solanum lycopersicum L). Potential of L. macroides in the promotion of S. lycopersicum L. growth by increased shoot length, terminal leaf length and breadth was assessed. Anatomical sectioning of stem and root revealed no varied cellular pattern indicating that the supplemented bioculture is not toxic to S. lycopersicum. Plantlets treated with L. macroides along with organic compost showed an increased total phenol content (17.58±0.4 mg/g) compared to control samples (12.44±0.41 mg/g). Carbohydrate content was noticed to be around 1.3 folds higher in the L. macroides plus compost mixture supplemented slots compared to control sample. Significant increase in shoot length was evident in the L. macroides plus compost supplied slots (23.4±2.7 cm). Plant growth promoting properties might be due to the nitrogen fixing activity of the bacteria which enrich the soil composition along with the nutrients supplied by the organic compost. Rich microbial rhizobiome diversity in pea plant and the usage of L. macroides from a non-conventional source improves the diversity of the available PGPR for agricultural practices. Further research is needed to detect the mechanism of growth promotion and to explore the plant microbe interaction pathway.

Isolation, identification and characterization of native plant growth promoting bacteria and their plant growth promotion in Zea mays

2021 ◽  
Vol 16 (8) ◽  
pp. 75-80
Author(s):  
Pitchaiah Pelapudi ◽  
Sasikala Ch ◽  
Swarnabala Ganti
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Root Length ◽  
Growth Promotion ◽  
Shoot Length ◽  
Root Weight ◽  
Plant Growth Promoting Bacteria ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Shoot Weight

In the present rapid growing world, need for a sustainable agricultural practice which helps in meeting the adequate food demand is much needed. In this context, plant growth promoting bacteria were brought into the spot light by the researchers. Though the plant growth promoting bacteria have several beneficial applications, due to some of the disadvantages in the field conditions, they lagged behind. In the current research work, native PGPR were isolated from the rhizosphere soil samples of maize with an aim to isolate the nitrogen fixing, phosphate solubilising and potash solubilising bacteria. Out of the several isolates, potent PGPR isolates viz., Paenibacillus durus PCPB067, Bacillus megaterium PCBMG041 and Paenibacillus glucanolyticus PCPG051 were isolated and identified by using the 16 S rRNA gene sequencing studies. Genomic DNA sequences obtained were deposited in the NCBI Genbank and accession numbers were assigned as MW793452, MW793456 and MW843633. In order to check the efficacy of the PGPR isolates, pot trials were conducted by taking maize as the host plant. Several parameters viz. shoot length, shoot weight, root length, root weight and weight of the seeds were tested in which PGP treatment showed good results (shoot length - 187±3.5 cm, shoot weight - 31±4 g, root length - 32±3.6 cm, root weight - 17±2 g, yield- 103.3±6.1 g) when compared to the chemical fertilizer treatment (shoot length - 177±3.5 cm, shoot weight - 25±3.6 g, root length - 24±3.5 cm, root weight - 14.6±1.52 g, yield- 85.6±7.6 g). Based on the results, it can be stated that these native PGPR isolates can be effectively used in the plant growth promotion of maize.

scholarly journals Preferential Promotion of Lycopersicon esculentum (Tomato) Growth by Plant Growth Promoting Bacteria Associated with Tomato

2014 ◽  
Vol 54 (4) ◽  
pp. 403-412 ◽  
Author(s):  
Papa Rao Vaikuntapu ◽  
Swarnalee Dutta ◽  
Ram Babu Samudrala ◽  
Vukanti R. V. N. Rao ◽  
Sadaf Kalam ◽  
...  
Keyword(s):  
Lycopersicon Esculentum ◽  
Plant Growth ◽  
Plant Growth Promoting Bacteria ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Tomato Growth

scholarly journals Plant Growth Promoting Ability of Soil Arsenite Resistant Bacteria

2016 ◽  
pp. 25-31
Author(s):  
Farzana Diba ◽  
Santonu Kumar Sannyal ◽  
SM Sabbir Alam ◽  
M Anwar Hossain ◽  
Munawar Sultana
Keyword(s):  
Plant Growth ◽  
Agricultural Land ◽  
Phosphate Solubilization ◽  
Resistant Bacteria ◽  
Soil Toxicity ◽  
Plant Growth Promoting Bacteria ◽  
Bacillus Sp ◽  
Iaa Production ◽  
Plant Growth Promoting ◽  
Growth Promoting

Arsenic (As) pollution in both soil and water is a global threat and challenge to us. Soil As contamination resulted in desolation of agricultural land and damaging human health via the food chain. Plant growth promoting bacteria (PGPB) enhance the ability of heavy metal/metalloid tolerance, increase biomass production in plant under adverse condition and also reduce the soil toxicity. This present study focuses on selection of the plant growth promoting trait within arsenite resistant bacteria, previously isolated from As contaminated soils of Faridpur district, Bangladesh. A total of 17 arsenite resistant bacteria belonging to 8 different genotypes comprising 6 different genera (Bacillus, Pseudomonas, Delftia, Wohlfahrtiimonas, Dietzia and Brevibacillus) were screened for their ability to produce plant growth promoting abilities such as indole acetic acid (IAA) production and phosphate solubilization (PS). Among 17 isolates, 15 93.75% (15/16) isolates produced IAA and 83.71% (12/14) isolates had the capacity to solubilize phosphate. Arsenite resistant Bacillus spp. were highly potent in both IAA production (33.33%) and phosphate solubilization (50%) followed by Pseudomonas spp. (40% IAA and 33.33% PS). High IAA production (91.5?g/ml) was exhibited by isolate Bacillus sp. A1b possessing a MICarsenite of 10 mM and maximum phosphate solubilization was observed by Bacillus sp. H2k. One of the arsenite resistant bacteria Detzia sp. H2f having 27 mM arsenite tolerance were able to produce IAA and showed maximum PS ability that was not previously reported. These isolates can be potential candidates for the enhancement of plant growth, provide protection of plants against As toxicity and be beneficial for sustainable agronomic production in As contaminated soils.Bangladesh J Microbiol, Volume 32, Number 1-2,June-Dec 2015, pp 25-31

Phosphate Solubilization and Plant Growth Promoting Actinobacteria from Rhizosphere Soil

2020 ◽  
Author(s):  
K. Balakrishnan ◽  
J. Thirumalairaj ◽  
M. Radhakrishnan ◽  
V. Gopikrishnan ◽  
R. Balagurunathan
Keyword(s):  
Plant Growth ◽  
Phosphatase Activity ◽  
Rhizosphere Soil ◽  
Phosphate Solubilization ◽  
Control Plant ◽  
Agar Plate ◽  
Plant Growth Promoting Bacteria ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Phosphate Solubilizing

Background: Phosphorus has been considered as the key element as it is directly involved in most of the life processes including in plants. Soil contains both organic and inorganic forms of phosphorus. Phosphate solubilization is the important property of soil bacteria to develop them as plant growth promoting bacteria in the agricultural field. The present study report the phosphate solubilizing and plant growth promoting properties of selected actinobacteria isolated from rhizosphere soil.Methods: Actinobacterial strains were isolated from rhizosphere soil and screened for in vitro phosphate solubilizing properties using agar plate method. The efficiency of phosphate solubilization and phosphatase activity of isolated actinobacterial strains were tested using Pikovskaya broth. Result: In the present study phosphate solubilization and phosphatase activity of isolated actinobacteria, Streptomyces sp. CTD-2 was comparatively higher in lab conditions. In pot trial experiment strain CTD-2 showed higher growth when compared to the control plant. The chlorophyll content of leaves in the experimental plants were found maximum with actinobacteria strain CTD-2 treated plant. Plant growth measurements such as root length, shoot length, leaf length, total plant growth measurements, quick yield production were also observed. 

Screening of Azotobacter strains from rice plant rhizosphere for phosphate solubilizing properties

2021 ◽  
Vol 27 (2) ◽  
Author(s):  
Niharika Dewangan ◽  
Shirin Anwar
Keyword(s):  
Plant Growth ◽  
Rice Plant ◽  
Phosphate Solubilization ◽  
Plant Growth Promoting Bacteria ◽  
Crop Fields ◽  
Rice Rhizosphere ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Phosphate Solubilizing ◽  
Phosphate Solubilizer

Azotobacter is a plant growth promoting bacteria which solubilize phosphorous and make it available for plants. In the present study the efforts have been taken to fulfill the aim to isolate, characterize and screen Azotobacter for phosphate solubilization from the rice rhizosphere of Durg district. Total 8 strains of Azotobacter were isolated from different rice fields. It was observed that all the strains were found to be phosphate solubilizing but in different quantity. RAZB6 is highest phosphate solubilizer (234.4±1mg/L) and RAZB4 shows minimum (82.09±1mg/L) quantity of phosphate solubilization. Since phosphorous is necessary for the plants proper development and it increases the growth of rice plant and make soil more fertile for other plants growth also so they must be commercialized and used more and more in the crop fields other than the agrochemicals.

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