Screening of Azotobacter strains from rice plant rhizosphere for phosphate solubilizing properties

2021 ◽  
Vol 27 (2) ◽  
Author(s):  
Niharika Dewangan ◽  
Shirin Anwar
Keyword(s):  
Plant Growth ◽  
Rice Plant ◽  
Phosphate Solubilization ◽  
Plant Growth Promoting Bacteria ◽  
Crop Fields ◽  
Rice Rhizosphere ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Phosphate Solubilizing ◽  
Phosphate Solubilizer

Azotobacter is a plant growth promoting bacteria which solubilize phosphorous and make it available for plants. In the present study the efforts have been taken to fulfill the aim to isolate, characterize and screen Azotobacter for phosphate solubilization from the rice rhizosphere of Durg district. Total 8 strains of Azotobacter were isolated from different rice fields. It was observed that all the strains were found to be phosphate solubilizing but in different quantity. RAZB6 is highest phosphate solubilizer (234.4±1mg/L) and RAZB4 shows minimum (82.09±1mg/L) quantity of phosphate solubilization. Since phosphorous is necessary for the plants proper development and it increases the growth of rice plant and make soil more fertile for other plants growth also so they must be commercialized and used more and more in the crop fields other than the agrochemicals.

Phosphate Solubilization and Plant Growth Promoting Actinobacteria from Rhizosphere Soil

2020 ◽  
Author(s):  
K. Balakrishnan ◽  
J. Thirumalairaj ◽  
M. Radhakrishnan ◽  
V. Gopikrishnan ◽  
R. Balagurunathan
Keyword(s):  
Plant Growth ◽  
Phosphatase Activity ◽  
Rhizosphere Soil ◽  
Phosphate Solubilization ◽  
Control Plant ◽  
Agar Plate ◽  
Plant Growth Promoting Bacteria ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Phosphate Solubilizing

Background: Phosphorus has been considered as the key element as it is directly involved in most of the life processes including in plants. Soil contains both organic and inorganic forms of phosphorus. Phosphate solubilization is the important property of soil bacteria to develop them as plant growth promoting bacteria in the agricultural field. The present study report the phosphate solubilizing and plant growth promoting properties of selected actinobacteria isolated from rhizosphere soil.Methods: Actinobacterial strains were isolated from rhizosphere soil and screened for in vitro phosphate solubilizing properties using agar plate method. The efficiency of phosphate solubilization and phosphatase activity of isolated actinobacterial strains were tested using Pikovskaya broth. Result: In the present study phosphate solubilization and phosphatase activity of isolated actinobacteria, Streptomyces sp. CTD-2 was comparatively higher in lab conditions. In pot trial experiment strain CTD-2 showed higher growth when compared to the control plant. The chlorophyll content of leaves in the experimental plants were found maximum with actinobacteria strain CTD-2 treated plant. Plant growth measurements such as root length, shoot length, leaf length, total plant growth measurements, quick yield production were also observed. 

scholarly journals Selective screening and characterization of plant growth promoting bacteria for growth enhancement of tomato, Lycopersicon esculentum

2021 ◽  
Author(s):  
Shiva Yavarian ◽  
Parvaneh Jafari ◽  
Neda Akbari ◽  
Mohammad Mehdi Feizabadi
Keyword(s):  
Lycopersicon Esculentum ◽  
Plant Growth ◽  
Tomato Plant ◽  
Phosphate Solubilization ◽  
Shoot Length ◽  
Plant Production ◽  
Plant Growth Promoting Bacteria ◽  
Dependent Manner ◽  
Plant Growth Promoting ◽  
Growth Promoting

Background and Objectives: Plant Growth-promoting Bacteria (PGPB) can replace the dangerous chemical fertilizers and pesticides. The aim of this study was to isolate the PGPBs for Lycopersicon esculentum plant and to determine the appropriate volume for inoculation. Materials and Methods: Plants samples were collected from tomato fields. Nitrogen fixing-PGPBs were isolated from rhizoplane and rhizosphere. Five isolates were screened based on their growth abilities and examined for PGPB traits including phosphate solubilization, and IAA, ammonia and HCN production. After high cell density cultivation, the cells were separated by centrifugation and freeze dried after resuspension in cryoprotectant. The powders were inoculated into sterile soil with a dose of 106 , 107 and 108 CFUs/g. Tomato (Lycopersicon esculentum) seeds were sown in soil and after 42 days the shoot length was measured. Results: Most of the potent PGPBs with high growth capacity were isolated from rhizoplane. Maximum phosphate solubilization was 289.7 µg/ml by NFB12 which isolated from rhizoplane. This strain produced the maximum level of IAA. NFB12 produced ammonia without the ability of production of HCN. This strain enhanced shoot length in dosed dependent manner. Surprisingly, inoculation of soil with 108 CFUs/g dramatically decreased the shoot length by 21%. Based on molecular approach NFB12 was identified as Bacillus megaterium. Conclusion: Isolation of specific PGPBS is recommended for sustainable plant production. Our results showed that NBF12 improves tomato plant growth and its effect on tomato plant growth is does dependent. Maximum growth rate of tomato was observed with 107 CFUs/g soil inoculation of NFB12 while higher inoculation showed negative effect.

scholarly journals Plant Growth Promoting Ability of Soil Arsenite Resistant Bacteria

2016 ◽  
pp. 25-31
Author(s):  
Farzana Diba ◽  
Santonu Kumar Sannyal ◽  
SM Sabbir Alam ◽  
M Anwar Hossain ◽  
Munawar Sultana
Keyword(s):  
Plant Growth ◽  
Agricultural Land ◽  
Phosphate Solubilization ◽  
Resistant Bacteria ◽  
Soil Toxicity ◽  
Plant Growth Promoting Bacteria ◽  
Bacillus Sp ◽  
Iaa Production ◽  
Plant Growth Promoting ◽  
Growth Promoting

Arsenic (As) pollution in both soil and water is a global threat and challenge to us. Soil As contamination resulted in desolation of agricultural land and damaging human health via the food chain. Plant growth promoting bacteria (PGPB) enhance the ability of heavy metal/metalloid tolerance, increase biomass production in plant under adverse condition and also reduce the soil toxicity. This present study focuses on selection of the plant growth promoting trait within arsenite resistant bacteria, previously isolated from As contaminated soils of Faridpur district, Bangladesh. A total of 17 arsenite resistant bacteria belonging to 8 different genotypes comprising 6 different genera (Bacillus, Pseudomonas, Delftia, Wohlfahrtiimonas, Dietzia and Brevibacillus) were screened for their ability to produce plant growth promoting abilities such as indole acetic acid (IAA) production and phosphate solubilization (PS). Among 17 isolates, 15 93.75% (15/16) isolates produced IAA and 83.71% (12/14) isolates had the capacity to solubilize phosphate. Arsenite resistant Bacillus spp. were highly potent in both IAA production (33.33%) and phosphate solubilization (50%) followed by Pseudomonas spp. (40% IAA and 33.33% PS). High IAA production (91.5?g/ml) was exhibited by isolate Bacillus sp. A1b possessing a MICarsenite of 10 mM and maximum phosphate solubilization was observed by Bacillus sp. H2k. One of the arsenite resistant bacteria Detzia sp. H2f having 27 mM arsenite tolerance were able to produce IAA and showed maximum PS ability that was not previously reported. These isolates can be potential candidates for the enhancement of plant growth, provide protection of plants against As toxicity and be beneficial for sustainable agronomic production in As contaminated soils.Bangladesh J Microbiol, Volume 32, Number 1-2,June-Dec 2015, pp 25-31

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