Induction of Hairy roots of Pegagan (Centella Asiatica (l.) Urban using Several Explant Sources with Several Agrobacterium Rhizogenes Strains in Vitro

2014 ◽  
Vol 4 (4) ◽  
pp. 286
Author(s):  
Zahanis Zahanis ◽  
Mansyurdin Mansyurdin ◽  
Zozy Aneloi Noli ◽  
Amril Bachtiar
Keyword(s):  
Agrobacterium Rhizogenes ◽  
Hairy Roots ◽  
Centella Asiatica ◽  
Agrobacterium Rhizogenes Strains

scholarly journals TÉCNICAS DE TRANSFORMAÇÃO E LINHAGENS De Agrobacterium rhizogenes NA PRODUÇÃO DE HAIRY ROOTS DE Ocimum basilicum L.

Nativa ◽  
2018 ◽  
Vol 6 (3) ◽  
pp. 266
Author(s):  
José Nicomedes Júnior ◽  
Roberta Cristiane Ribeiro ◽  
Luis Louro Berbara ◽  
Elvia Mariam Lis Martinez Stark ◽  
Wagner Campos Otoni ◽  
...  
Keyword(s):  
Agrobacterium Rhizogenes ◽  
Hairy Roots ◽  
Leaf Explants ◽  
Laboratory Strain ◽  
Root Culture ◽  
Ocimum Basilicum ◽  
Best Response ◽  
Agrobacterium Rhizogenes Strains ◽  
Whole Plants

O manjericão é uma planta medicinal e seus metabólitos especiais são utilizados com fins terapêuticos e industriais. Este trabalho teve por objetivo avaliar técnicas e linhagens de Agrobacterium rhizogenes na produção de raízes transformadas de duas variedades de Ocimum basilicum L., Dark Opal (roxo) e Minete Anão (verde). Verificou-se o efeito da inoculação com linhagens de A. rhizogenes selvagens (A4, 8196, 9402, 2659, 2659 G, 17242, LBA, 15834) ou transformadas (R1601) em plantas inteiras ou explantes (folhas e segmentos de caule) de manjericão, cultivados em casa de vegetação ou in vitro. A inoculação com as linhagens LBA e 8196 possibilitou redução no número de explantes de folha de manjericão-verde oxidados, quando comparado ao controle. Dentre as linhagens selvagens, as que proporcionaram melhor indução na produção de raízes transformadas foram A4, LBA e 8196, respectivamente. Entretanto, a cepa R1601 apresentou a melhor resposta. Raízes foram eficientemente induzidas mediante a inoculação de explantes de manjericão-roxo e manjericão-verde e expressaram o fenótipo típico de raízes em cabeleira (hairy roots). As culturas clonais de manjericão-verde apresentaram rápido crescimento em meio de cultura livre de reguladores de crescimento.Palavras-chave: agrobactéria, cultura de raízes, manjericão, plantas medicinais. TRANSFORMATION TECHNIQUES AND Agrobacterium rhizogenes Strains In The Production Of Hairy Roots Of Ocimum basilicum L. ABSTRACT:Basil is a medicinal plant and its special metabolites are used for therapeutic or industrial purposes. The aim of this work was to evaluate Agrobacterium rhizogenes techniques and strains in the production of hairy roots of two varieties of Ocimum basilicum L., Dark Opal (purple) and Dwarf Racer (green). The effects of inoculation of A. rhizogene (A4, 8196, 9402, 2659, 2659, 2659 G, 17242, LBA, 15834) or (R1601) lines on whole plants or explants (leaves and stem segments) of basil, grown in a greenhouse or in vitro, in the production of hairy roots and calluses made by subcultures. Inoculation with the LBA and 8196 lines allowed a reduction in the number of oxidized basil-green leaf explants when compared to the control. Among the wild strains, those that had best ability to induce hairy root of hairy roots were A4, LBA and 8196, respectively. However, the R1601 laboratory strain had the best response. Roots were efficiently induced by the inoculation of explants basil-purple and basil-green and expressed the typical phenotype of hairy roots. Green-basal clonal cultures showed rapid growth in culture medium free of growth regulators.Keywords: agrobacterim, basil, medicinal plants, root culture. DOI:

scholarly journals Establishment of Highly Efficient Agrobacterium Rhizogenes-mediated Transformation for Stevia Rebaudiana Bertoni Explants

2016 ◽  
Vol 58 (1) ◽  
pp. 113-118 ◽  
Author(s):  
Żaneta Michalec-Warzecha ◽  
Laura Pistelli ◽  
Francesca D’Angiolillo ◽  
Marta Libik-Konieczny
Keyword(s):  
Agrobacterium Rhizogenes ◽  
Hairy Roots ◽  
Molecular Level ◽  
Stevia Rebaudiana ◽  
Culture Conditions ◽  
Light Conditions ◽  
Inoculum Concentration ◽  
Stevia Rebaudiana Bertoni ◽  
Pcr Method

Abstract Leaves and internodes from Stevia rebaudiana Bertoni plants growing in different conditions were used for transformation with two strains of Agrobacterium rhizogenes: ATCC 15384 and LBA 9402. Hairy roots formation was observed and the percentage of the transformed explants depended on the type of explant, time of inoculation and inoculum concentration. Inoculation of explants from ex vitro and in vitro plants with LBA 9402 strain led to higher efficiency of transformation than inoculation with ATCC 15384 strain. Growth rate of hairy roots in liquid culture was assessed under light and dark conditions. It was found that the growth of hairy roots decreased significantly under light conditions. Transformation of hairy roots growing in different culture conditions was confirmed at the molecular level using PCR method with primers constructed against rolB and rolC genes from A. rhizogenes.

Effects of Agrobacterium rhizogenes strains and other parameters on production of isoflavonoids in hairy roots of Pueraria candollei Grah. ex Benth. var. candollei

2012 ◽  
Vol 111 (3) ◽  
pp. 315-322 ◽  
Author(s):  
Pimsiri Danphitsanuparn ◽  
Panitch Boonsnongcheep ◽  
Thanaphol Boriboonkaset ◽  
Yupyn Chintapakorn ◽  
Sompop Prathanturarug
Keyword(s):  
Agrobacterium Rhizogenes ◽  
Hairy Roots ◽  
Pueraria Candollei ◽  
Agrobacterium Rhizogenes Strains

The Use of Hairy Roots Transformed by Agrobacterium Rhizogenes for the Production of Plant Secondary Products in Vitro

1988 ◽  
pp. 425-429
Author(s):  
John D. Hamill ◽  
Adrian J. Parr ◽  
Cathie Martin ◽  
Nick J. Walton ◽  
Richard Robins ◽  
...  
Keyword(s):  
Agrobacterium Rhizogenes ◽  
Hairy Roots ◽  
Secondary Products

scholarly journals Induction of transgenic hairy roots in soybean genotypes by Agrobacterium rhizogenes‑mediated transformation

2011 ◽  
Vol 46 (9) ◽  
pp. 1070-1075 ◽  
Author(s):  
Ricardo Luís Mayer Weber ◽  
Maria Helena Bodanese‑Zanettini
Keyword(s):  
Agrobacterium Rhizogenes ◽  
Hairy Root ◽  
Hairy Roots ◽  
Fluorescent Protein ◽  
Root Culture ◽  
Transformation Frequency ◽  
Plant Production ◽  
Uniform Size ◽  
Soybean Genotypes

The objective of this work was to perform the screening of soybean genotypes as to their ability to respond to the induction of hairy roots by Agrobacterium rhizogenes‑mediated transformation. Four Brazilian soybean cultivars (BRSMG 68 Vencedora, BRS 137, Embrapa 48, and MG/BR 46 Conquista) and two North American ones adapted to Brazilian cropping conditions (Bragg and IAS‑5) were screened for their capacity to respond to A. rhizogenes in protocols for in vitro hairy root culture and ex vitro composite plant production. Four‑day‑old seedlings with uniform size were injected with A. rhizogenes harboring the plasmid p35S‑GFP. Seedlings expressing green fluorescent protein (GFP) in at least one hairy root were used to determine the transformation frequency. Using an axenic in vitro protocol, excised cotyledons from four‑day‑old seedlings were infected with A. rhizogenes harboring the pCAMBIA1301 plasmid, containing the gusA reporter gene. The transformation frequency and the number of days for hairy root emergence after bacterial infection (DAI) were evaluated. The transformation frequency and DAI varied according to the genotype. Cultivars MG/BR 46 Conquista and BRSMG 68 Vencedora are more susceptible to A. rhizogenes and can be recommended for transformation experiments.

scholarly journals PRODUÇÃO DE RAÍZES TRANSFORMADAS DE PIMENTA-LONGA POR INFECÇÃO COM AGROBACTERIUM RHIZOGENES

2017 ◽  
Vol 18 (4) ◽  
pp. 179
Author(s):  
José Nicomedes Júnior ◽  
Roberta Cristiane Ribeiro ◽  
Ricardo Luis Louro Berbara ◽  
Elvia Mariam Lis Martinez Stark ◽  
Wagner Campos Otoni ◽  
...  
Keyword(s):  
Agrobacterium Rhizogenes ◽  
Hairy Roots

Este trabalho teve por objetivo a obtenção de raízes transgênicas (hairy roots) de Piper hispidinervium (pimenta-longa), espécie reconhecida pela produção de óleo essencial rico em Safrol, metabólito secundário utilizado com fins terapêuticos e industrial. As raízes foram produzidas a partir da infecção de explantes in vitro de pimenta-longa com Agrobacterium rhizogenes (Estirpe R1601). Os resultados mostraram que a estirpe R1601 de A. rhizogenes foi eficiente para inoculação baseado na imersão de explantes em meio de cultura liquido, com a bactéria em crescimento, o que representou maior praticidade, menor índice de contaminação, maior taxa de infecção e menores danos aos explantes. A resposta morfológica foi a mesma entre os clones, não permitindo a distinção dos mesmos. Explantes de folha de pimenta-longa, inoculados com a estirpe R1601, formaram raízes que apresentaram crescimento nos subcultivos subseqüentes. Foi possível realizar a extração de óleo essencial produzido pela cultura de raízes de forma semelhante a plantas sob cultivo, sendo o metanol, o melhor solvente extrator do óleo essencial produzido.

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