scholarly journals BIORREMOÇÃO DE FENANTRENO POR BIOMASSA VIVA E INATIVADA CUNNINGHAMELLA ELEGANS UCP0542

e-xacta ◽  
2013 ◽  
Vol 6 (1) ◽  
pp. 1 ◽  
Author(s):  
Marta Cristina Freitas Silva ◽  
Adriana Almeida Antunes ◽  
Clarissa Isabel Matos Lins ◽  
Antonio Helder Parente ◽  
Sônia Valéria Pereira ◽  
...  
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Cunninghamella Elegans ◽  
Biotechnological Potential ◽  
Independent Variables ◽  
C Elegans ◽  
Live Biomass ◽  
Polycyclic Aromatic ◽  
Full Factorial ◽  
Inactivated Biomass

<p align="justify">Realizou-se estudos avaliando a ação da biomassa viva e inativada de Cunninghamella elegans crescida, visando à remoção do hidrocarboneto aromático policíclico fenantreno. Monitorou-se o processo de remoção do fenantreno a cada 24 h por espectrofotometria, de acordo com um planejamento fatorial completo de 22 e, como variáveis independentes 0,1 e 0,2 mM de fenantreno, na presença de 0,6, 3,3 e 6% de glicose e variável resposta o conteúdo de fenantreno removido. Os resultados obtidos indicaram que tanto a biomassa inativada como viva removeram, respectivamente, 90,0% e 82,0% a concentração de 0,2mM de fenantreno na presença da menor concentração de glicose (0,6%). A biomassa inativada de C. elegans demonstrou ser um sorbente com elevado potencial biotecnológico para a remediação de áreas poluídas com hidrocarbonetos aromáticos policíclicos.</p><p align="justify">Abstract</p><p align="justify">Studies were carried out evaluating the action of the life and inactivated biomass of Cunninghamella elegans grown to removal polycyclic aromatic hydrocarbon phenanthrene. The removal process was monitored each 24h by spectrophotometry, de acordo com according to full factorial design 2 2 , and independent variables the phenanthrene concentrations [0.1 and 0.2 mM], and the content of phenanthrene removed as variable response.The results showed that both inactivated and live biomass from C. elegans removed 85.0% and 90.0%, respectively, of phenantrene of 0.2 mM on glucose (0.6) concentration. The inactivated biomass from C. elegans shows a sorbent with high biotechnological potential for bioremediation of polluted area with polycyclic aromatic hydrocarbons.</p>

Transformation of chrysene and other polycyclic aromatic hydrocarbon mixtures by the fungus Cunninghamella elegans

1995 ◽  
Vol 73 (S1) ◽  
pp. 1025-1033 ◽  
Author(s):  
Jairaj V. Pothuluri ◽  
Allison Selby ◽  
Frederick E. Evans ◽  
James P. Freeman ◽  
Carl E. Cerniglia
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbon ◽  
Aromatic Hydrocarbons ◽  
High Performance ◽  
Parent Compound ◽  
Environmental Pollutants ◽  
Health Concern ◽  
Cunninghamella Elegans ◽  
C Elegans ◽  
Polycyclic Aromatic

Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous and persistent environmental pollutants; some are mutagenic, toxic, and carcinogenic and remain a public health concern. We investigated the metabolism of mixtures of PAHs and a tetracyclic aromatic hydrocarbon, chrysene, by the filamentous fungus, Cunninghamella elegans ATCC 36112. Cunninghamella elegans metabolized a mixture of PAHs including the carcinogen benzo[a]pyrene, phenanthrene, fluoranthene, pyrene, and acenaphthene completely to hydroxylated intermediates within 24 h. The metabolites from the PAH mixtures were similar to those formed in earlier studies of individual PAH compounds. In separate experiments with chrysene, C. elegans metabolized about 45% of the [5,6,11,12-14C]chrysene added to cultures during 144 h incubation. The two major metabolites of chrysene were separated by reverse-phase high performance liquid chromatography and identified by ultraviolet–visible, mass spectral, and 1H-nuclear magnetic resonance techniques as sulfate conjugates of 2,8-dihydroxychrysene and 2-hydroxychrysene. The two major metabolites accounted for about 33% of the total metabolism. The formation of sulfate conjugates of phenolic chrysene metabolites and glucoside conjugates and hydroxylated products of PAH mixtures by C. elegans may be a detoxification step, because these types of products are generally less toxic than the parent compound. Key words: polycyclic aromatic hydrocarbons, PAH mixtures, chrysene, Cunninghamella elegans, biotransformation, oxidation.

A green analytical method for the analysis of polycyclic aromatic hydrocarbons in oral fluids from crack smokers

Bioanalysis ◽  
2020 ◽  
Vol 12 (24) ◽  
pp. 1711-1724
Author(s):  
Pavla Lôbo Rossiter ◽  
Lucas José de Alencar Danda ◽  
Maria Fernanda Pimentel ◽  
Danielle Cristine Almeida Silva de Santana ◽  
Fernando José Malagueño de Santana
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Solid Phase Microextraction ◽  
Oral Fluid ◽  
Solid Phase ◽  
Oral Fluids ◽  
Relative Standard ◽  
Polycyclic Aromatic ◽  
Relative Errors ◽  
Full Factorial

Aim: Develop and validate a method of solid-phase microextraction (SPME) and liquid chromatography to investigate three major polycyclic aromatic hydrocarbons (PAHs) in oral fluid. Results/Methodology: The extraction phase was exposed to 1.5 ml of diluted oral fluid under stirring at 1000 rpm for 60 min, at 70°C. Then, it was immersed in 200 μl of acetonitrile for 10 min at 25°C for desorption of the analytes. Linearity, absolute recovery, and inter- and intra-assay relative standard deviations and relative errors were 50–300 ng.ml-1, ≥24% and ≤15% for all analytes, respectively. A full factorial design was used to SPME optimization. Discussion/Conclusion: The method is suitable for the exploratory analysis of some PAHs in the oral fluid of crack smokers.

The Assessment of Contamination and Source of Polycyclic Aromatic Hydrocarbons from the Sediments of the Someş River

2019 ◽  
Vol 64 (1) ◽  
pp. 55-67
Author(s):  
Vlad Pӑnescu ◽  
◽  
Mihaela Cӑtӑlina Herghelegiu ◽  
Sorin Pop ◽  
Mircea Anton ◽  
...  
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Polycyclic Aromatic

1,1’-Biaceanthrylene and 2,2'-Biaceanthrylene: Models for Linking Larger Polycyclic Aromatic Hydrocarbons via Five-Membered Rings

2019 ◽  
Author(s):  
Yachu Du ◽  
Kyle Plunkett
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Polycyclic Aromatic Hydrocarbon ◽  
Aromatic Hydrocarbon ◽  
Electronic Properties ◽  
Aromatic Hydrocarbons ◽  
Model Systems ◽  
Redox Potentials ◽  
Dimer Model ◽  
Optical And Electronic Properties ◽  
Polycyclic Aromatic

We show that polycyclic aromatic hydrocarbon (PAH) chromophores that are linked between two five-membered rings can access planarized structures with reduced optical gaps and redox potentials. Two aceanthrylene chromophores were connected into dimer model systems with the chromophores either projected outward (2,2’-biaceanthrylene) or inward (1,1’-biaceanthrylene) and the optical and electronic properties were compared. Only the planar 2,2’-biaceanthrylene system showed significant reductions of the optical gaps (1 eV) and redox potentials in relation to the aceanthrylene monomer.<br>

1,1’-Biaceanthrylene and 2,2'-Biaceanthrylene: Models for Linking Larger Polycyclic Aromatic Hydrocarbons via Five-Membered Rings

2019 ◽  
Author(s):  
Yachu Du ◽  
Kyle Plunkett
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Polycyclic Aromatic Hydrocarbon ◽  
Aromatic Hydrocarbon ◽  
Electronic Properties ◽  
Aromatic Hydrocarbons ◽  
Model Systems ◽  
Redox Potentials ◽  
Dimer Model ◽  
Optical And Electronic Properties ◽  
Polycyclic Aromatic

We show that polycyclic aromatic hydrocarbon (PAH) chromophores that are linked between two five-membered rings can access planarized structures with reduced optical gaps and redox potentials. Two aceanthrylene chromophores were connected into dimer model systems with the chromophores either projected outward (2,2’-biaceanthrylene) or inward (1,1’-biaceanthrylene) and the optical and electronic properties were compared. Only the planar 2,2’-biaceanthrylene system showed significant reductions of the optical gaps (1 eV) and redox potentials in relation to the aceanthrylene monomer.<br>

EMISSION OF POLYCYCLIC AROMATIC HYDROCARBONS CONTAINED IN COMBUSTION PRODUCTS OF GASOLINE ENGINES

2018 ◽  
Vol 62 (3) ◽  
pp. 341-346 ◽  
Author(s):  
M. Assad ◽  
V. V. Grushevski ◽  
O. G. Penyazkov ◽  
I. N. Tarasenko
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
High Speed ◽  
Internal Combustion Engines ◽  
Catalytic Converter ◽  
Combustion Products ◽  
Chromatography Method ◽  
Exhaust Gases ◽  
Polycyclic Aromatic ◽  
Load Mode

The concentration of 16 polycyclic aromatic hydrocarbons (PAHs) in the gasoline combustion products emitted into the atmosphere by internal combustion engines (ICE) has been measured using the gas chromatography method. The concentrations of PAHs in the exhaust gases sampled behind a catalytic converter has been determined when the ICE operates in five modes: idle mode, high speed mode, load mode, ICE cold start mode (engine warm-up) and transient mode. Using 92 RON, 95 RON and 98 RON gasoline the effect of the octane number of gasoline on the PAHs content in the exhaust gases has been revealed. The concentration of the most carcinogenic component (benzo(α)pyrene) in the exhaust gases behind a catalytic converter significantly exceeds a reference value of benzo(α)pyrene in the atmospheric air established by the WHO and the EU for ICE in the load mode.

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