Molecular Characterization and Assessment of Hematobiochemical and Oxidative Indices in Dog Naturally Infected with Babesia canis vogeli

2021 ◽  
Author(s):  
Prabhakar Shil ◽  
Jayesh B. Solanki ◽  
Niranjan Kumar ◽  
Dharmesh C. Patel ◽  
Nabanita Thakuria
Keyword(s):  
Plasma Cortisol ◽  
18S Rrna ◽  
Gene Sequence ◽  
Molecular Detection ◽  
18S Rrna Gene ◽  
Mean Value ◽  
Serum Glucose ◽  
Rrna Gene ◽  
Babesia Canis ◽  
The Mean

Background: The study was aimed at molecular detection and assessment of important biomarkers in the natural cases of canine babesiosis. Methods: Blood samples of 239 dogs were examined in PCR by targeting 18S rRNA gene. Hematobiochemical, oxidant-antioxidant and plasma cortisol parameters were estimated in the dogs on the day of presentation. Result: The 18S rRNA gene sequence showed 100% homology with Babesia canis vogeli and phylogram formed a tight cluster of B. canis vogeli originated from India/other countries. Higher prevalence rate (P less than 0.05) was noted in the PCR (7.95%) than the cytological technique (3.76%). Hemogram of infected dogs showed decrease (P less than 0.05) in the mean value of hemoglobin, RBC, WBC, HCT, whereas an increase in MCHC, lymphocytes, eosinophils, monocytes and thrombocytes. The ALT (49.29±1.53 U/L), AST (48.33±2.93 U/L), total protein (10.56±0.60 g/dL), creatinine (1.41±0.10 mg/dL) and urea (19.32±0.97 mg/dL) showed significant (P less than 0.005) increase, whereas decrease in the levels of serum glucose (82.76±2.78 mg/dL) in the infected dogs. Activity of MDA and SOD was significantly (P less than 0.01) increased (7.50±7.08 nmole/µL blood) and decreased (0.015±3.91 nmole/µL blood) in the diseased dogs, respectively. Plasma cortisol concentration was 11.10±7.84 nmol/L and 2.77±5.78 nmol/L (P less than 0.01) in the infected and uninfected dogs, respectively.

Leptochlorella corticola gen. et sp. nov. and Kalinella apyrenoidosa sp. nov.: two novel Chlorella-like green microalgae (Trebouxiophyceae, Chlorophyta) from subaerial habitats

2013 ◽  
Vol 63 (Pt_1) ◽  
pp. 377-387 ◽  
Author(s):  
Jiří Neustupa ◽  
Yvonne Němcová ◽  
Jana Veselá ◽  
Jana Steinová ◽  
Pavel Škaloud
Keyword(s):  
18S Rrna ◽  
New Genus ◽  
Gene Sequence ◽  
Novel Species ◽  
Algal Species ◽  
18S Rrna Gene ◽  
Tree Bark ◽  
Rrna Gene ◽  
Green Microalgae ◽  
Green Algal

The diversity of green microalgae in subaerial habitats remains largely unexplored and a number of new genus- and species-level lineages have been discovered recently. The traditional green algal genus, Chlorella, which accommodated coccoid unicellular green algal species with globular to oval cells, reproducing entirely by autospores, has been found to be polyphyletic. In this study, we provide a detailed characterization of two strains of microalgae isolated from tree bark in the Mediterranean. These algae share the general Chlorella-like morphology and their 18S rRNA and rbcL gene sequences place them in the Trebouxiophyceae. Strain CAUP H8401 forms an independent trebouxiophycean lineage, together with three previously published 18S rRNA gene environmental sequences of undescribed microalgae, which were retrieved from profoundly different habitats. In contrast, strain CAUP H7902 is related to Kalinella bambusicola in the Watanabea clade of the Trebouxiophyceae on the basis of its 18S rRNA gene sequence. This relationship is also supported by the rbcL gene sequence, acquired from the type strain of K. bambusicola. The investigated strains are described as representatives of a novel species in a new genus, Leptochlorella corticola gen. et sp. nov., and a novel species, Kalinella apyrenoidosa sp. nov., according to the International Code of Nomenclature for Algae, Fungi and Plants.

scholarly journals Correction to: A comprehensive fungi-specific 18S rRNA gene sequence primer toolkit suited for diverse research issues and sequencing platforms

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Stefanos Banos ◽  
Guillaume Lentendu ◽  
Anna Kopf ◽  
Tesfaye Wubet ◽  
Frank Oliver Glöckner ◽  
...  
Keyword(s):  
Fungal Community ◽  
18S Rrna ◽  
Gene Sequence ◽  
18S Rrna Gene ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Community Surveys ◽  
Research Issues ◽  
Nomenclature System ◽  
Sequencing Platforms

Following publication of the original article [1], we have been notified that three of the primer names identified as most promising candidates for fungal community surveys were incorrectly renamed following the primer nomenclature system proposed by Gargas & DePriest [2].

scholarly journals Phylogeny of Intestinal Ciliates, Including Charonina ventriculi, and Comparison of Microscopy and 18S rRNA Gene Pyrosequencing for Rumen Ciliate Community Structure Analysis

2015 ◽  
Vol 81 (7) ◽  
pp. 2433-2444 ◽  
Author(s):  
Sandra Kittelmann ◽  
Savannah R. Devente ◽  
Michelle R. Kirk ◽  
Henning Seedorf ◽  
Burk A. Dehority ◽  
...  
Keyword(s):  
Community Structure ◽  
High Throughput ◽  
18S Rrna ◽  
Gene Sequence ◽  
18S Rrna Gene ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Content Type ◽  
Advantages And Disadvantages ◽  
Ciliate Community

ABSTRACTThe development of high-throughput methods, such as the construction of 18S rRNA gene clone or pyrosequencing libraries, has allowed evaluation of ciliate community composition in hundreds of samples from the rumen and other intestinal habitats. However, several genera of mammalian intestinal ciliates have been described based only on morphological features and, to date, have not been identified using molecular methods. Here, we isolated single cells of one of the smallest but widely distributed intestinal ciliates,Charonina ventriculi, and sequenced its 18S rRNA gene. We verified the sequence in a full-cycle rRNA approach using fluorescencein situhybridization and thereby assigned an 18S rRNA gene sequence to this species previously known only by its morphology. Based on its full-length 18S rRNA gene sequence,Charonina ventriculiwas positioned within the phylogeny of intestinal ciliates in the subclass Trichostomatia. The taxonomic framework derived from this phylogeny was used for taxonomic assignment of trichostome ciliate 18S rRNA gene sequence data stemming from high-throughput amplicon pyrosequencing of rumen-derived DNA samples. The 18S rRNA gene-based ciliate community structure was compared to that obtained from microscopic counts using the same samples. Both methods allowed identification of dominant members of the ciliate communities and classification of the rumen ciliate community into one of the types first described by Eadie in 1962. Notably, each method is associated with advantages and disadvantages. Microscopy is a highly accurate method for evaluation of total numbers or relative abundances of different ciliate genera in a sample, while 18S rRNA gene pyrosequencing represents a valuable alternative for comparison of ciliate community structure in a large number of samples from different animals or treatment groups.

Reassessment of the classification of the Order Haplosclerida (Class Demospongiae, Phylum Porifera) using 18S rRNA gene sequence data

2007 ◽  
Vol 43 (1) ◽  
pp. 344-352 ◽  
Author(s):  
N.E. Redmond ◽  
R.W.M. van Soest ◽  
M. Kelly ◽  
J. Raleigh ◽  
S.A.A. Travers ◽  
...  
Keyword(s):  
18S Rrna ◽  
Gene Sequence ◽  
Sequence Data ◽  
18S Rrna Gene ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Gene Sequence Data

scholarly journals Identification of Theileria parva and Theileria sp. (buffalo) 18S rRNA gene sequence variants in the African Buffalo (Syncerus caffer) in southern Africa

2011 ◽  
Vol 182 (2-4) ◽  
pp. 150-162 ◽  
Author(s):  
Mamohale E. Chaisi ◽  
Kgomotso P. Sibeko ◽  
Nicola E. Collins ◽  
Fred T. Potgieter ◽  
Marinda C. Oosthuizen
Keyword(s):  
Southern Africa ◽  
18S Rrna ◽  
Gene Sequence ◽  
18S Rrna Gene ◽  
Rrna Gene ◽  
Sequence Variants ◽  
Theileria Parva ◽  
African Buffalo ◽  
Syncerus Caffer ◽  
Rrna Gene Sequence

Characterization of two novel yeast strains used in mediated biosensors for wastewater

2002 ◽  
Vol 48 (5) ◽  
pp. 418-426 ◽  
Author(s):  
Steve P Trosok ◽  
John H.T Luong ◽  
David F Juck ◽  
Brian T Driscoll
Keyword(s):  
18S Rrna ◽  
Gene Sequence ◽  
Phylogenetic Analyses ◽  
Pulp Mill ◽  
18S Rrna Gene ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Candida Spp ◽  
Yeast Strains ◽  
Physiological Tests

After isolation from a pulp mill wastewater treatment facility, two yeast strains, designated SPT1 and SPT2, were characterized and used in the development of mediated biochemical oxygen demand (BOD) biosensors for wastewater. 18S rRNA gene sequence analysis revealed a one nucleotide difference between the sequence of SPT1 and those of Candida sojae and Candida viswanthii. While SPT2 had the highest overall homology to Pichia norvegensis, at only 73.5%, it is clearly an ascomycete, based on BLAST comparisons and phylogenetic analyses. Neighbor-joining dendrograms indicated that SPT1 clustered with several Candida spp., and that SPT2 clustered with Starmera spp., albeit as a very deep branch. Physiological tests, microscopic observations, and fatty acid analysis confirmed that SPT1 and SPT2 are novel yeast strains. Physiological tests also indicated that both strains had potential for use in mediated biosensors for estimation of BOD in wastewater. The lower detection limits of SPT1- and SPT2-based K3Fe(CN)6-mediated biosensors for a pulp-mill effluent were 2 and 1 mg BOD/L, respectively. Biosensor-response times for effluents from eight different pulp mills were in the range of 5 min. Reliability and sensitivity of the SPT1- and SPT2-based biosensors were good, but varied with the wastewater.Key words: yeast characterization, 18S rRNA gene sequence, pulp-mill wastewater, BOD5, mediated BOD biosensor.

scholarly journals The Evolutionary History of the Genus Acanthamoeba and the Identification of Eight New 18S rRNA Gene Sequence Types

1998 ◽  
Vol 45 (1) ◽  
pp. 45-54 ◽  
Author(s):  
Diane R. Stothard ◽  
Jill M. Schroeder-Diedrich ◽  
Mohammad H. Awwad ◽  
Rebecca J. Gast ◽  
Dolena R. Ledee ◽  
...  
Keyword(s):  
Evolutionary History ◽  
18S Rrna ◽  
Gene Sequence ◽  
18S Rrna Gene ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
History Of ◽  
Sequence Types

scholarly journals First Molecular Detection of Babesia gibsoni in Stray Dogs from Thailand

Pathogens ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 639
Author(s):  
Thom Do ◽  
Ruttayaporn Ngasaman ◽  
Vannarat Saechan ◽  
Opal Pitaksakulrat ◽  
Mingming Liu ◽  
...  
Keyword(s):  
18S Rrna ◽  
Molecular Detection ◽  
Genetic Characterization ◽  
18S Rrna Gene ◽  
Rrna Gene ◽  
Babesia Gibsoni ◽  
Its1 Region ◽  
Stray Dogs ◽  
Southern Thailand ◽  
Vector Borne

In southern Thailand, the increasingly growing population of stray dogs is a concern to public health and environmental safety because of the lack of medical attention and control. More importantly, these animals are considered reservoirs for many zoonotic pathogens. The objective of this study was to molecularly detect canine vector-borne pathogens, and to perform genetic characterization of Babesia gibsoni present in stray dogs from southern Thailand. Blood samples were collected from 174 stray dogs in two provinces (Songkhla and Narathiwat) in southern Thailand. PCR analyses were executed using specific primers based on the Babesia spp. 18S rRNA gene, Babesia gibsoni Internal transcribed spacer 1 (ITS1) region, Ehrlichia canis citrate synthase (gltA) gene, Hepatozoon spp. 18S rRNA gene and Anaplasma platys heat shock protein (groEL) gene. The most common canine vector-borne pathogen found infecting stray dogs in this study was Hepatozoon canis (24.7%) followed by A. platys (14.9%), Babesia vogeli (8.0%), B. gibsoni (6.3%), and E. canis (1.72%). Concurrent infection with more than one pathogen occurred in 72 cases. Phylogenetic analysis based on the ITS1 region and 18S rRNA gene revealed that the B. gibsoni isolates from this study shared a large proportion of their identities with each other and with other reported B. gibsoni genotypes from Asia. This study highlights the molecular detection of B. gibsoni in dogs in Thailand for the first time and presents the genetic characterization by sequencing the ITS1 region and 18S rRNA gene of B. gibsoni from Thailand. Follow-up studies are needed to elucidate the origin, distribution, and vectors of B. gibsoni parasites circulating in dogs in Thailand, as well as to determine to what extent dogs are important reservoir hosts for zoonotic canine vector-borne disease infection in the studied area.

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