Isolation and Molecular Characterization of Turkey Pox Virus from Maharashtra, India: A Review

2021 ◽  
Author(s):  
M.M. Pawade ◽  
P.P. Mhase ◽  
D.M. Muglikar ◽  
V.D. Lonkar ◽  
P.V. Mehere ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Virus Isolation ◽  
Viral Infections ◽  
Genetic Characterization ◽  
Phylogenetic Analyses ◽  
Chain Reaction ◽  
Slow Spreading ◽  
Polymerase Chain ◽  
Avian Pox

Avian pox diseases are contagious and slow spreading viral infections in birds. The present study was aim to, isolate and molecular characterization of turkeypox virus from a clinical case. Ten out of the twelve scab lesions sample collected from clinically suspected cases were positive for avian pox viurs (APV) based on virus isolation and polymerase chain reaction. We conducted genetic characterization of the APV strain. The phylogenetic analyses of P4b gene APV genome indicated that, avian poxviruses fragments sequenced in this study clustered along the A clade of avipoxviruses, genetically related to Indian fowl pox virus isolated from chicken, showing 99% homology.

Molecular characterization of canine parvovirus in Brazil by polymerase chain reaction assay

2000 ◽  
Vol 75 (2) ◽  
pp. 127-133 ◽  
Author(s):  
Cesar A.D Pereira ◽  
Telma A Monezi ◽  
Dolores U Mehnert ◽  
Magali D’Angelo ◽  
Edison L Durigon
Keyword(s):  
Polymerase Chain Reaction ◽  
Molecular Characterization ◽  
Polymerase Chain Reaction Assay ◽  
Canine Parvovirus ◽  
Chain Reaction ◽  
Polymerase Chain

Rapid molecular characterization of Hb H disease in Chinese by polymerase chain reaction

1994 ◽  
Vol 68 (1) ◽  
pp. 33-37 ◽  
Author(s):  
J. G. Chang ◽  
T. C. Liu ◽  
L. I. Perng ◽  
S. S. Chiou ◽  
T. P. Chen ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Molecular Characterization ◽  
Chain Reaction ◽  
Hb H Disease ◽  
Polymerase Chain

scholarly journals The Molecular Characterization of Intestinal Explant HIV Infection Using Polymerase Chain Reaction-Based Techniques

2015 ◽  
Vol 31 (10) ◽  
pp. 981-991 ◽  
Author(s):  
Laura Janocko ◽  
Andrew D. Althouse ◽  
Rhonda M. Brand ◽  
Ross D. Cranston ◽  
Ian McGowan
Keyword(s):  
Polymerase Chain Reaction ◽  
Hiv Infection ◽  
Molecular Characterization ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Genetic diversity of Blastocystis in kindergarten children in southern Xinjiang, China

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Meng Qi ◽  
Zilin Wei ◽  
Ying Zhang ◽  
Qiyuan Zhang ◽  
Juanfeng Li ◽  
...  
Keyword(s):  
Ribosomal Rna ◽  
Genetic Characterization ◽  
Intestinal Parasites ◽  
Small Subunit ◽  
Kindergarten Children ◽  
Chain Reaction ◽  
Significant Difference ◽  
Polymerase Chain ◽  
Southern Xinjiang

Abstract Background Blastocystis is one of the most common intestinal parasites in humans and various animals worldwide. Few studies are available regarding the genetic characterization of Blastocystis infections in humans in China. Methods In the present study, 609 fecal samples were collected from two- to six-year-old kindergarten children in southern Xinjiang and were examined by polymerase chain reaction (PCR). Results The infection rate of Blastocystis was 14.3% (87/609); no significant difference was observed among counties and between sexes. Blastocystis subtypes ST1 (n = 38), ST2 (n = 8), and ST3 (n = 41) were identified by sequence analysis of the small subunit ribosomal RNA gene. Genetic polymorphisms were observed at the intra-subtype level, including seven variations for ST1 (ST1A to ST1G), four for ST2 (ST2A to ST2D), and two for ST3 (ST3A and ST3B); with ST1F and ST2B being new variations. Conclusions ST1 and ST3 are the two common Blastocystis subtypes in the study area. More extensive studies in both humans and animals in different regions are needed to better characterize the transmission of Blastocystis.

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