scholarly journals Isolation and characterization of plant growth promoting endophytic bacteria isolated from Vigna radiata

2018 ◽  
Author(s):  
Pooja Suneja ◽  
Rajat Maheshwari ◽  
Namita Bhutani
Keyword(s):  
Plant Growth ◽  
Vigna Radiata ◽  
Endophytic Bacteria ◽  
Fungal Pathogens ◽  
Growth Promotion ◽  
Restriction Analysis ◽  
Isolation And Characterization ◽  
Plant Growth Promoting ◽  
Growth Promoting

A total of 22 endophytic bacteria were isolated from roots and nodules of Vigna radiata (mungbean) obtained from Jind district, Haryana. These were characterized on the basis of plant growth promoting traits. Almost all the endophytic bacteria produced IAA with maximum production of 81.63µg/ml by isolate MJiR8. Among these, 100% root isolates and 84.6% nodule isolates resulted in in vitro root growth promotion of mungbean seedlings. All the isolates produced ammonia; eighteen (all root and nine nodule isolates) produced organic acid while only four root isolates were positive for siderophore production. The four isolates produced hydrogen cyanide and out of these only MJiR9 inhibited the growth of fungal pathogens Fusarium oxysporium and Aspergillus niger. All the endophytes were used to determine molecular diversity by ARDRA (Amplified Ribosomal DNA Restriction Analysis) Results revealed that the nodule isolates were more diverse, being present in separate clusters, in comparison to root isolates which were grouped together in cluster III.

scholarly journals Isolation and Characterization of Bacterial Endophytes from Lycopersicon esculentum Plant and their Plant Growth Promoting Characteristics

1970 ◽  
Vol 2 (1) ◽  
pp. 37-52 ◽  
Author(s):  
Hardik A Patel ◽  
Rajesh K Patel ◽  
Sunil M Khristi ◽  
Kruti Parikh ◽  
Geetha Rajendran
Keyword(s):  
Lycopersicon Esculentum ◽  
Plant Growth ◽  
Endophytic Bacteria ◽  
Growth Promotion ◽  
Bacterial Endophytes ◽  
Isolation And Characterization ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Different Characteristics

The study was designed to isolate and characterize bacterial endophytes from root and stem of Lycopersicon esculentum plant which was collected form different region of Gujarat. Total 18 isolates of endophytic bacteria were selected in which, all the endophytic bacteria produced one or the other different characteristics involved in plant growth promotion. They either produced phytohormones like indole acetic acid, siderophore, protease, pectinase, organic acid showed antifungal activity, chromium tolerance and solubilized phosphate. Four of the strains among the 18 showed maximum positive results of plant growth promoting regulators (PGPR) test and among them best probable isolate was selected and thus its 16SrDNA was amplified and sequenced. Only HR7 endophyte of tomato turned out to be Pseudomonas aeruginosa. It’s a gram negative coccobacili, sporeforming motile bacilli and show maximum PGPR activity. The results of our present studies indicated that above strains might be endophytic and therefore, were associated with the plant growth. Keywords: Lycopersicon esculentum; endophytic bacteria; PGPR; IAA; 16SrDNA DOI: http://dx.doi.org/10.3126/njb.v2i1.5679   Nepal Journal of Biotechnology Jan.2012, Vol.2(1): 37-52

scholarly journals Isolation and Characterization of Salt Tolerant Endophytic and Rhizospheric Plant Growth-Promoting Bacteria (PGPB) Associated with the Halophyte Plant (Sesuvium Verrucosum) Grown in KSA

2015 ◽  
Vol 3 (3) ◽  
pp. 552-560 ◽  
Author(s):  
Mohamed A.M. El-Awady ◽  
Mohamed M. Hassan ◽  
Yassin M. Al-Sodany
Keyword(s):  
Nitrogen Fixation ◽  
Acetic Acid ◽  
Plant Growth ◽  
Growth Promotion ◽  
Exponential Growth Phase ◽  
Plant Growth Promoting Bacteria ◽  
Salt Tolerant ◽  
Isolation And Characterization ◽  
Growth Promoting

This study was designed to isolate and characterize endophytic and rhizospheric bacteria associated with the halophyte plant Sesuvium verrucosum, grown under extreme salinity soil in Jeddah, Saudi Arabia. The plant growth promotion activities of isolated bacterial were evaluated in vitro. A total of 19 salt tolerant endophytic and rhizospheric bacterial isolates were obtained and grouped into six according to genetic similarity based on RAPD data. These six isolates were identified by amplification and partial sequences of 16S rDNA as Enterobacter cancerogenus,Vibrio cholerae, Bacillus subtilis, Escherichia coli and two Enterobacter sp. Isolates were then grown until exponential growth phase to evaluate the atmospheric nitrogen fixation, phosphate solubilization, and production of phytohormones such as indole-3-acetic acid, as well as 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity. While, All of the six strains were negative for ACC deaminaseactivity, two isolates showed Nitrogen fixation activity, three isolates produce the plant hormone (Indole acetic acid) and two isolates have the activity of solubiliztion of organic phosphate. Among the six isolates, the isolate (R3) from the soil around the roots is able to perform the three previous growth promoting possibilities together and it is ideal for use in promoting the growth of plants under the high salinity conditions. This isolate is candidate to prepare a friendly biofertelizer that can be used for the improvement of the crops performance under salinity conditions.Int J Appl Sci Biotechnol, Vol 3(3): 552-560

scholarly journals Isolation and characterization of plant growth-promoting endophytic bacteria Bacillus stratosphericus LW-03 from Lilium wardii

3 Biotech ◽  
2020 ◽  
Vol 10 (7) ◽  
Author(s):  
Mohammad Sayyar Khan ◽  
Junlian Gao ◽  
Mingfang Zhang ◽  
Xuqing Chen ◽  
The Su Moe ◽  
...  
Keyword(s):  
Plant Growth ◽  
Endophytic Bacteria ◽  
Isolation And Characterization ◽  
Plant Growth Promoting ◽  
Growth Promoting

scholarly journals Characterization of bacterial endophytes from the roots of native and cultivated Brazil nut trees (Bertholletia excelsa)

2019 ◽  
Vol 49 (4) ◽  
pp. 257-267
Author(s):  
Patrícia Bombonati CHALITA ◽  
Eliane do Nascimento Cunha FARIAS ◽  
Ismaele Breckenfeld da COSTA ◽  
Brenda Ferreira SOUSA ◽  
Marco Antônio Oliveira dos SANTOS ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Endophytic Bacteria ◽  
Growth Promotion ◽  
Nifh Gene ◽  
Aluminum Phosphate ◽  
Brazil Nut ◽  
Indole Compounds ◽  
Plant Growth Promoting ◽  
Growth Promoting

ABSTRACT Brazil nut is a very important nontimber forest product in the Amazon region. Propagation of this tree still represents a challenge due to slow and uneven seed germination. In this context, plant growth-promoting bacteria can facilitate the process of propagation. The aims of this study were to isolate and characterize endophytic bacteria from the roots of Brazil nut trees in native terra firme forest and cultivation areas in northern Brazil, and to identify mechanisms by which bacteria act in plant growth promotion. Overall, 90 bacterial isolates were obtained from the roots of Brazil nut trees in monoculture, agroforestry and native forest areas by using different semisolid media. The isolates were characterized by sequencing the 16S rRNA gene. Plant growth-promoting characteristics were evaluated by the presence of the nifH gene, aluminum phosphate solubilization and the production of indole compounds. The isolates were affiliated with 18 genera belonging to 5 different classes (α-Proteobacteria, β-Proteobacteria, γ-Proteobacteria, Bacilli and Actinobacteria). The genus Bacillus was predominant in the forest and monoculture areas. Fourteen isolates presented the nifH gene. Most of the bacteria were able to solubilize aluminum phosphate and synthetize indole compounds. The results indicated high diversity of endophytic bacteria present among the roots of Brazil nut trees, mainly in the agroforestry area, which could be related to soil attributes. Among the 90 isolates, the 22 that presented the best results regarding plant growth promotion traits were good candidates for testing in seedling production of Brazil nut trees.

scholarly journals Isolation and characterization of plant growth-promoting endophytic bacteria of wild legumes growing on sandy soils of Binh Thuan province, Vietnam

2021 ◽  
Vol 10 (3) ◽  
pp. 246-254
Author(s):  
Dang Thi Ngoc Thanh ◽  
Pham Thi Thu Ly ◽  
Pham Thi Nga ◽  
Pham Van Ngot
Keyword(s):  
Nitrogen Fixation ◽  
Plant Growth ◽  
Endophytic Bacteria ◽  
Phosphate Solubilization ◽  
Sandy Soils ◽  
Legume Species ◽  
Bacterial Isolates ◽  
Isolation And Characterization ◽  
Plant Growth Promoting ◽  
Growth Promoting

The roots of two legume species (Tephrosia purpurea and Tephrosia villosa) that grew wild on dry sandy soils of Binh Thuan province were sources for isolating plant growth-promoting endophytic bacteria. Semi-solid LGI medium was used for the isolation of nitrogen-fixing bacteria from root extracts. All bacterial isolates isolates were evaluated for their ability to solubilize calcium orthophosphate on solid NBRIP medium and their ability to produce IAA in Burk's liquid medium supplemented with 100 mg/L tryptophan. The possibilities of nitrogen fixation, phosphate solubilization and IAA synthesis were all quantitative examined by colorimetric method. Twenty-two bacterial isolates of T. purpurea and 18 isolates of T. villosa were capable of nitrogen fixation in the range of 1.94 to 2.81 mg/L NH4+, whereas only 18 isolates of T. purpurea and 16 isolates of T. villosa showed phosphate solubilization in the range of 12.30 – 48.90 mg/L P2O5, and IAA production in the range of 0.38 – 12.72 mg/L. Sixteen outstanding bacterial isolates of the two legume species were identified by MALDI-TOF technique. The results showed that 13 isolates had high similarity with five bacterial genera including Klebsiella, Cronobacter, Enterobacter, Burkholderia, and Bacillus with score values in the range of 2.070 – 2.411.

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