scholarly journals PHENOTYPIC COMPOSITION OF PERIPHERAL BLOOD LYMPHOCYTES AND THEIR COOPERATION IN PATIENTS WITH CHRONIC MERCURY INTOXICATION IN A POST-CONTACT PERIOD

2019 ◽  
Vol 98 (10) ◽  
pp. 1091-1095
Author(s):  
Elena V. Boklazhenko ◽  
G. M. Bodienkova
Keyword(s):  
Immune System ◽  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Contact Period ◽  
Blood Lymphocytes ◽  
Mercury Intoxication ◽  
System Functioning ◽  
Therapeutic Measures ◽  
Chronic Mercury Intoxication

Introduction. Based on the current understanding of the progression of professional chronic mercury intoxication, it is extremely important to study the regulatory activity of immunocompetent cells after the cessation contact with mercury to develop effective therapeutic measures. The purpose of the study was to study the population and subpopulation spectrum of peripheral blood lymphocytes and their cooperation in patients with chronic mercury intoxication in a distant postexposure period. Material and methods. Phenotyping of lymphocytes in the blood of the subjects was carried out by the method of indirect immunofluorescence using monoclonal antibodies to the molecules CD3+, CD4+, CD8+, CD9+, CD16+, CD20+, CD21+, CD23+, CD25+, CD95+. Results. In patients with chronic mercury intoxication in the long-term postexposure period, there were revealed features of the immune system functioning, indicating hyperactivation of both T- and B-components of the immune system. An increase in the total population of T-lymphocytes was established due to an increase in cells with receptors CD4+ (T-lymphocyte-helper cells) and CD16+ (killer cells), as well as an increase in the number of mature B-lymphocytes (CD20+) and pre-B-immature-lymphocytes (CD9+). Changes in the system of lymphocyte apoptosis, characterized by an increase in the number of cells expressing receptors for readiness for Fas-dependent apoptosis (CD95+), have been recorded. The established relationships between populations and subpopulations of lymphocytes indicate their importance in the implementation of the immune response, high activity and contingency between the components of the immune system in persons with chronic mercury intoxication after the termination of contact with the toxicant. Conclusion. The results obtained are the basis for long-term monitoring of the health status and improvement of the tactics of treating patients with neurointoxication with mercury in the postexposure period.

scholarly journals Assessing the level of chromosome aberrations in peripheral blood lymphocytes in long-term resident children under conditions of high exposure to radon and its decay products

Mutagenesis ◽  
2015 ◽  
Vol 30 (5) ◽  
pp. 677-683 ◽  
Author(s):  
Vladimir G. Druzhinin ◽  
Maxim Yu. Sinitsky ◽  
Aleksey V. Larionov ◽  
Valentin P. Volobaev ◽  
Varvara I. Minina ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Chromosome Aberrations ◽  
Peripheral Blood Lymphocytes ◽  
High Exposure ◽  
Blood Lymphocytes ◽  
Decay Products

scholarly journals Cytokine production by peripheral blood lymphocytes and their LAK cell activity in patients on short- or long-term hemodialysis.

1993 ◽  
Vol 26 (2) ◽  
pp. 183-189
Author(s):  
Masamichi Hayakawa ◽  
Tadashi Hatano ◽  
Masami Oda ◽  
Kunio Yoshihara ◽  
Shinichiro Yoshi ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Cytokine Production ◽  
Cell Activity ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes ◽  
Lak Cell ◽  
Lak Cell Activity

scholarly journals CD16- CD56+ natural killer cells after bone marrow transplantation

Blood ◽  
1992 ◽  
Vol 79 (12) ◽  
pp. 3239-3244 ◽  
Author(s):  
R Jacobs ◽  
M Stoll ◽  
G Stratmann ◽  
R Leo ◽  
H Link ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Immune System ◽  
Bone Marrow Transplantation ◽  
Nk Cells ◽  
Natural Killer ◽  
Peripheral Blood ◽  
Marrow Transplantation ◽  
Nk Cell ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes

Abstract Natural killer (NK) cells are phenotypically defined as lymphocytes expressing the antigens CD56 and mostly CD16 (Fc gamma RIII), but lacking CD3. A small CD3- CD16- CD56+ NK cell subset has been described in normal individuals representing less than 2% of peripheral blood lymphocytes. We analyzed here 70 patients for their reconstitution of the immune system during follow-up after autologous or allogeneic bone marrow transplantation. In 35% of these patients, two different NK cell subsets, namely CD56+dim and CD56+bright cells, were observed. The mean duration of these two subsets after transplant was 4 months. Sixty-five percent of the patients exhibited an increased number of NK cells, but only the typical CD16+ CD56+dim population. The CD56+bright subpopulation represented a particular CD3- CD16- NK subset, with posttransplant frequencies up to 70% of all NK cells and 40% of peripheral blood lymphocytes, respectively. In contrast to normal CD56+dim NK cells, CD56+bright cells coexpressed the activation antigens p75 beta-chain of interleukin-2 receptor (IL-2R), CD2R, and CD26, but were negative for CD16. NK and antibody-dependent cellular cytotoxicity activity of CD56+bright cells was low compared with CD56+dim NK cells. But using IL-2 and interferon gamma, their cytotoxicity could be enhanced even more than in CD56+dim lymphocytes. These different subsets may reflect distinct activation or differentiation steps of NK cells during reconstitution of the immune system. Their differential response to IL-2 may be of functional importance for posttransplant cytokine therapy.

Stress-Related Immune Activity in Tension-Type Headache: Analysis of Immune System-Associated Antigen Expressed on Peripheral Blood Lymphocytes

Cephalalgia ◽  
1991 ◽  
Vol 11 (11_suppl) ◽  
pp. 360-361
Author(s):  
Keiko Yamamoto ◽  
Toshimi Ikeda ◽  
Kiyoshi Yamamoto ◽  
Yoshitaka Takase ◽  
Toshihiro Fukusako ◽  
...  
Keyword(s):  
Immune System ◽  
Psychological Stress ◽  
Patient Group ◽  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Tension Type Headache ◽  
Immune Activity ◽  
Blood Lymphocytes ◽  
Type Headache

In order to evaluate the effect of psychological stress on immune activity, we examined immune system-associated antigen expressed on peripheral blood lymphocytes in tension-type headache. We found differences between the highly stressed patient group and the minimally stressed. These results support the concept that psychological stress my significantly alter the immune activity in patients suffering from tension-type headache depending upon whether they are highly stressed or not.

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