IMMUNOCHROMATOGRAPHIC TEST FOR DETERMINATION OF D-DIMER

2019 ◽  
Vol 64 (11) ◽  
pp. 654-658
Author(s):  
Yulia Aleksandrovna Akinshina ◽  
A. V. Nikitina ◽  
E. A. Amelina ◽  
S. G. Mardanly
Keyword(s):  
Venous Thromboembolism ◽  
Blood Plasma ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Diagnostic Strategy ◽  
Plasma Samples ◽  
Immunochromatographic Test ◽  
Qualitative Determination ◽  
D Dimer

The developing and the testing results of an immunochromatographic test for the D-dimer qualitative determination were presents in the article. The test was approved blood plasma samples in comparison with a quantitative enzyme-linked immunosorbent assay (ELISA). The results of assay with developed test were the same with ELISA results for 87,1% and 100% for samples with increased (more than 400 ng/ml FEU) and normal concentration of D-dimer, respectively. The immunochromatographic test for determination of D-dimer can be included in the diagnostic strategy as a cut test after the assessment of venous thromboembolism risk.

Determination of Human Thrombin-Antithrombin III Complex in Plasma with an Enzyme-Linked Immunosorbent Assay

1988 ◽  
Vol 59 (01) ◽  
pp. 101-106 ◽  
Author(s):  
Hermann Pelzer ◽  
Angela Schwarz ◽  
Norbert Heimburger
Keyword(s):  
Immunosorbent Assay ◽  
Antithrombin Iii ◽  
Test System ◽  
Mean Value ◽  
Enzyme Linked Immunosorbent Assay ◽  
Plasma Samples ◽  
Vein Thrombosis ◽  
Human Thrombin ◽  
Thrombin Antithrombin Iii Complex

SummaryAn enzyme-linked immunosorbent assay (ELISA) was developed for the determination of thrombin-antithrombin III complex (TAT) in human plasma. The test system follows the sandwich principle and uses two different antibodies directed against human thrombin and human antithrombin III, respectively. The antibodies bind selectively to the corresponding antigen moieties of TAT. The assay was calibrated with definite concentrations of preformed purified TAT added to TAT-poor plasma. The lower limit of sensitivity of the assay was 0.5 μg/1. Mean coefficients of variation of 4.2% (intraassay) and 3.5% (interassay) were found for TAT concentrations between 2 and 60 μg/1. A reference range from 0.85 to 3.2 μg/1 was calculated from TAT concentration in plasma samples from 88 healthy donors (mean value ± SD: 1.45 ± 0.4 μg/I). In plasma samples from patients with pulmonary embolism (n = 17), TAT concentrations between 3 and 25 μg/1 were measured. In 15 patients with deep vein thrombosis, TAT was found up to 3 to 25 μg/1. From these data we conclude that measurement of TAT can be a sensitive parameter for specific detection of a latent activation of the clotting pathway.

Simultaneous determination of CRP and D-dimer in human blood plasma samples with White Light Reflectance Spectroscopy

2016 ◽  
Vol 84 ◽  
pp. 89-96 ◽  
Author(s):  
Georgios Koukouvinos ◽  
Panagiota Petrou ◽  
Konstantinos Misiakos ◽  
Dimitris Drygiannakis ◽  
Ioannis Raptis ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Simultaneous Determination ◽  
Human Blood ◽  
White Light ◽  
Reflectance Spectroscopy ◽  
Human Blood Plasma ◽  
Plasma Samples ◽  
Light Reflectance ◽  
D Dimer

SENSITIVE AND SPECIFIC ENZYME-LINKED IMMUNOSORBENT ASSAY FOR UROKINASE-TYPE PLASMINOGEN ACTIVATOR IN HUMAN PLASMA

1987 ◽  
Author(s):  
J Grøndahl-HANSEN ◽  
N Agerlin ◽  
L S Nielsen ◽  
K Danø
Keyword(s):  
Plasminogen Activator ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Mean Values ◽  
Amino Terminal ◽  
Type Plasminogen Activator ◽  
Healthy Donors ◽  
Urokinase Type Plasminogen Activator ◽  
The Mean

An enzyme-linked immunosorbent assay (ELISA) was developed for the measurement of human urokinase-type plasminogen activator (u-PA) in plasma and serum. Microtiter plates were coated with a monoclonal antibody and incubated with standard or sample. Bound u-PA was quantitated with polyclonal antibodies conjugated with biotin, followed by avidin-peroxidase. The assay was 10-fold as sensitive as other previously reported ELISAs, the detection limit being approximately 1 pg of u-PA in a volume of 100 μl with a linear dose-response up to 15 pg of u-PA. The assay detected active u-PA and its inactive proenzyme form equally well and the recovery of both forms was higher than 90% in plasma. A variety of structurally related proteins, including t-PA, were tested, but no reaction with proteins other than u-PA and its amino-terminal degradation product were observed. The intra-assay and inter-assay coefficients of variation for determination of u-PA in plasma were 7.6% and 8.4%, respectively. The assay was equally applicable to serum. The values obtained with plasma and serum were similar, and the results were not affected by small variations in the preparation of the samples. The ELISA was used to measure the concentration of u-PA in plasma from 34 healthy donors. The mean values for u-PA in plasma from healthy donors was 1.1 ng/ml ± 0.3 ng/ml (SD) (range 0.6 - 1.5 ng/ml). No significant differences were found between men and women and no correlation between u-PA concentration and age could be demonstrated.The mean u-PA concentration in plasma from healthy donors obtained in this study is substantially lower than that reported by others. This might be due to different methods of determination of the protein content of the standard preparations or to differences in the specificity of the assays.

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