Simultaneous determination of CRP and D-dimer in human blood plasma samples with White Light Reflectance Spectroscopy

A multiple reaction monitoring liquid chromatography – mass spectrometry method for the simultaneous determination of five antiretroviral drugs in human blood plasma.

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The results of testing the laboratory version of the testkit for determination of HBsAg subtypes and hepatitis B virus genotypes in human blood plasma samples using ELISA

Molecular Genetics Microbiology and Virology (Russian version) ◽

10.17116/molgen202038041188 ◽

2020 ◽

Vol 38 (4) ◽

pp. 188

Author(s):

L.V. Bezuglova ◽

V.A. Manuilov ◽

L.P. Osipova ◽

Ya.D. Mosina ◽

V.A. Poryvaeva ◽

...

Keyword(s):

Hepatitis B Virus ◽

Hepatitis B ◽

Blood Plasma ◽

Human Blood ◽

Human Blood Plasma ◽

Plasma Samples ◽

B Virus ◽

Virus Genotypes

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Simultaneous determination of aromatic amino acids in human blood plasma by capillary electrophoresis with UV-absorption detection

Journal of Separation Science ◽

10.1002/jssc.201500038 ◽

2015 ◽

Vol 38 (10) ◽

pp. 1794-1799 ◽

Cited By ~ 13

Author(s):

Mauro Forteschi ◽

Salvatore Sotgia ◽

Stefano Assaretti ◽

Dionigia Arru ◽

Debora Cambedda ◽

...

Keyword(s):

Amino Acids ◽

Capillary Electrophoresis ◽

Blood Plasma ◽

Simultaneous Determination ◽

Human Blood ◽

Aromatic Amino Acids ◽

Uv Absorption ◽

Human Blood Plasma

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Development and validation of an improved HPLC-UV method for simultaneous determination of lamotrigine and oxcarbazepine and its active metabolite 10,11-dihydro-10-hydroxycarbazepine in human blood plasma and comparison with an UHPLC-MS/MS method

Journal of Analytical Science & Technology ◽

10.1186/s40543-019-0198-9 ◽

2019 ◽

Vol 10 (1) ◽

Cited By ~ 1

Author(s):

Siyao Jin ◽

Qing Zhao ◽

Dongjie Zhang ◽

Zhigang Zhao ◽

Shenghui Mei

Keyword(s):

Liquid Chromatography ◽

Blood Plasma ◽

Simultaneous Determination ◽

Human Blood ◽

High Performance ◽

Internal Standard ◽

Ultra Performance Liquid Chromatography ◽

Human Blood Plasma ◽

Elution Time

AbstractLamotrigine (LTG) and oxcarbazepine (OXC) are first-line drugs for epilepsy treatment. Their large pharmacokinetics variabilities and relations between efficacy and toxicity and blood plasma concentration require routine monitoring for dose adjustment. In this study, we developed and validated a simple, accurate, and reliable method for simultaneous determination of LTG, OXC and 10,11-dihydro-10-hydroxycarbazepine (MHD) in human blood plasma by high-performance liquid chromatography-ultraviolet detection (HPLC-UV) with a simple one-step protein precipitation using methanol (1% acetic acid) and 15 min elution time under isocratic elution at 1 mL/min. Calibration range was 2.4 to 120 mg/L for LTG, OXC, and MHD. The intra-day and inter-day bias were − 8.84 to 4.18%, and the imprecision was less than 8.08% for all analytes. The internal standard (fluconazole) normalized recovery was 96.30 to 107.69% for LTG, 98.51 to 111.04% for MHD, and 95.04 to 109.86% for OXC. A total of 186 LTG samples and 25 MHD samples were used to evaluate the agreement between HPLC-UV and ultra-performance liquid chromatography-mass spectrometry (UHPLC-MS/MS) by Passing-Bablok regression and Bland-Altman plot. The mean bias and the 95% limits of agreement (95% LOA) of the two measurements were 0.575 mg/L and − 1.238 to 2.387 mg/L for LTG (n = 186) and − 1.222 mg/L and − 8.271 to 5.827 mg/L for MHD (n = 25), which indicated the UV method was comparable with the MS method for LTG and MHD analysis.

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