Proinflammatory and Anti-Inflammatory Cytokines in Gingival Crevicular Fluid and Serum of Patients With Rheumatoid Arthritis and Patients With Chronic Periodontitis

In the recent years, statistically significant associations between rheumatoid arthritis (RA) and periodontal disease have been identified. Emerging as a chronic inflammatory joint disease, RA displays various features and pathogenetic events similar to chronic periodontitis (CP). The purpose of this study was to evaluate the utility of determining systemic and crevicular levels of metalloproteinase-9 (MMP-9) as potential biomarkers for association between RA and CP. A total of fifty-six patients were included in the study. The subjects were categorized into four groups as follows: healthy-control (n=21), active RA (n=16), CP (n=14), and RA-CP association (n=12). Assessment of serum and crevicular concentrations of total MMP-9 (active and pro-MMP-9) was based on ELISA technique. The results of this study showed statistically significant differences of serum MMP-9 between patients groups and control. Serum levels of MMP-9 were similar in RA and RA-CP associated patients. Gingival crevicular fluid (GCF) recorded increased MMP-9 levels in RA-CP association subjects as compared to CP. Considering that RA-CP association is characterized by a disregulation of the inflammatory response, MMP-9 may play a role in the pathogenesis of RA-CP association. MMP-9 is therefore a sensitive tool in the diagnosis and management of patients affected by this binomial association.

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Effect of laser-assisted scaling and root planing on the expression of pro-inflammatory cytokines in the gingival crevicular fluid of patients with chronic periodontitis: A systematic review

Photodiagnosis and Photodynamic Therapy ◽

10.1016/j.pdpdt.2017.02.010 ◽

2017 ◽

Vol 18 ◽

pp. 63-77 ◽

Cited By ~ 13

Author(s):

Sergio Varela Kellesarian ◽

Vanessa Ros Malignaggi ◽

Hasham Abdullah Majoka ◽

Abdulaziz A. Al-Kheraif ◽

Tammy Varela Kellesarian ◽

...

Keyword(s):

Systematic Review ◽

Inflammatory Cytokines ◽

Chronic Periodontitis ◽

Gingival Crevicular Fluid ◽

Scaling And Root Planing ◽

Root Planing ◽

Crevicular Fluid ◽

Pro Inflammatory Cytokines

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Decreased Interleukin-1β and Elastase in the Gingival Crevicular Fluid of Individuals Undergoing Anti-Inflammatory Treatment for Rheumatoid Arthritis

Journal of Periodontology ◽

10.1902/jop.2007.060520 ◽

2007 ◽

Vol 78 (8) ◽

pp. 1612-1619 ◽

Cited By ~ 22

Author(s):

Letícia A. Miranda ◽

Alexandre G. Islabão ◽

Ricardo G. Fischer ◽

Carlos M.S. Figueredo ◽

Rui V. Oppermann ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Gingival Crevicular Fluid ◽

Interleukin 1Β ◽

Anti Inflammatory ◽

Crevicular Fluid

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Cytokine profile in the gingival crevicular fluid of rheumatoid arthritis patients with chronic periodontitis

Journal of Investigative and Clinical Dentistry ◽

10.1111/jicd.12066 ◽

2013 ◽

Vol 5 (1) ◽

pp. 1-8 ◽

Cited By ~ 19

Author(s):

Fawad Javed ◽

Hameeda B. Ahmed ◽

Toshinari Mikami ◽

Khalid Almas ◽

Georgios E. Romanos ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Chronic Periodontitis ◽

Gingival Crevicular Fluid ◽

Cytokine Profile ◽

Crevicular Fluid

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Beneficial Effects of Glycyrrhizin in Chronic Periodontitis Through the Inhibition of Inflammatory Response

Dose-Response ◽

10.1177/1559325820952660 ◽

2020 ◽

Vol 18 (3) ◽

pp. 155932582095266

Author(s):

Yaru Sun ◽

Binghui Zhao ◽

Zhaobao Li ◽

Jianming Wei

Keyword(s):

Inflammatory Cytokines ◽

Chronic Periodontitis ◽

Gingival Crevicular Fluid ◽

High Mobility ◽

Major Constituent ◽

Beneficial Effects ◽

Tnf Α ◽

Periodontal Ligament Stem Cell ◽

Glycyrrhiza Glabra L ◽

Crevicular Fluid

Elevated inflammatory cytokines and high mobility group box 1 (HMGB1) production are associated with chronic periodontitis (CP). Glycyrrhizin is the major constituent of Glycyrrhiza glabra. L. (Fabaceae) root with anti-inflammation activities. This study evaluated the effects of glycyrrhizin on CP. TNF-α-treated human periodontal ligament stem cell (hPDLSC) model was established, and was administrated with 1, 2 or 5 mM glycyrrhizin for 24 h. After treatment, the expression of HMGB1and inflammatory cytokines was monitored. Significantly increased HMGB1 (median: 5646.4, range: 1918.2-8233.7 vs median: 204.5, range: 98.7-283.6, pg/mL), TNF-α (median: 345.5, range: 161.0-567.9 vs median: 93.5, range: 58.1-159.3, pg/mL), IL-1β (median: 2014.6, range: 209.5-4308.1 vs median: 224.5, range: 48.8-335.8, pg/mL) and IL-6 (median: 1223.6, range: 398.2-2183.8 vs median: 240.4, range: 105.2-400.5, pg/mL) were detected in gingival crevicular fluid from CP patients. Glycyrrhizin significantly prevented TNF-α-induced expression of HMGB1 (691.5 ± 136.4 vs 142.8 ± 57.3 pg/mL), IL-6 (388.1 ± 85.2 vs 189.4 ± 61.2 pg/mL) and IL-1β (176.3 ± 47.2 vs 53.9 ± 25.7 pg/mL) in hPDLSC. In CP rats, glycyrrhizin significantly decreased HMGB1 (5795.6 ± 1121.5 vs 586.4 ± 436.8 pg/mL), TNF-α (421.8 ± 93.7 vs 87.9 ± 21.6 pg/mL), IL-6 (1423.8 ± 235.2 vs 622.6 ± 176.1 pg/mL) and IL-1β (1562.8 ± 334.3 vs 733.5 ± 265.1 pg/mL) in gingival crevicular fluid. Glycyrrhizin suppresses inflammatory activities in CP rats and represents a promising molecule for controlling CP.

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